Gene ID:HHVTW001.1
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
Gene Name:
Orf-P
Definition:
unknown
Cellular Location:
[Evidence]
Gene Start:
1231
Gene Stop:
839
Gene Length:
393
Molecular Weight*:
13595
pI*:
12.80
Net Charge*:
26.13
EC:
Functional Class:
Gene Ontology:
Pathway: pathway table
Secondary Evidence:
Randall G, Lagunoff M, Roizman B, Herpes simplex virus 1 ORF O and ORF P are not necessary for the establishment of latent imfection in mice, J Virol 2000 Oct;74(19):9019-27., Medline: 10982346.
Roizman B, HSV gene functions: what have we learned that could be
generally applicable to its near and distant cousins? Acta Virol 1999 Apr-Jun;43(2-3):75-80, Medline: 20161414.
Randall G, Roizman B, Transcription of the derepressed open reading frame P of herpes simplex virus 1 precludes the expression of the antisense gamma(1)34.5 gene and may account for the attenuation of the mutant virus, J Virol 1997 Oct;71(10):7750-7, Medline: 97456546.
Lagunoff M, Randall G, Roizman B, Phenotypic properties of herpes simplex virus 1 containing a derepressed open reading frame P gene, J Virol 1996 Mar;70(3):1810-7, Medline: 96190583.
Lagunoff M, Roizman B, The regulation of synthesis and properties of the protein product of open reading frame P of the herpes simplex virus 1 genome, J Virol 1995 Jun;69(6):3615-23, Medline:
95264452.
Lagunoff M, Roizman B, Expression of a herpes simplex virus 1
open reading frame antisense to the gamma(1)34.5 gene and transcribed by an RNA 3' coterminal with the unspliced latency-associated transcript, J Virol 1994 Sep;68(9):6021-8, Medline: 94335119.
Comment:
This is a hypothetical protein, defined by nucleotide similarity to it's HSV-1 counterpart. It has 75-85% C-G content over it's length.
Orf-P is antisense to the gamma34.5 gene and part of the 8.5 kb latency transcribed transcript (LAT). Lagunoff and Roizman(1995) report that in HSV-1 it is repressed during productive infection by binding of ICP4 to it's cap site. The Orf P protein is localized in splicesomes and binds p32, a component of the ASF/SF2 alternate splicing factors. This binding blocks synthesis of viral proteins from sliced mRNA, which includes ICP0 (alpha0) and UL15. By blocking synthesis of ICP0, a powerful viral gene transactivator, Orf-P may play a role in establishing or maintaining latency.
Orf-P is dispensible for growth in cell culture, see Roizman, PNAS 93:11307 (1996).
The HSV-2 genome contains two copies of Orf-P one wholly within the long terminal repeat, RL region, (this location) and one in the inverted repeat region separating the long and short unique regions.
Blast Summary: PSI-Blast Search
No similarity to HSV-2 proteins. The first 144 nucleotides of Orf-P show 84% similarity to nucleotides in Human herpesvirus 1 at genomic coordinates 125185-125328 and 1186-1043.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
Residues 7-33 may represent block BL00048, Protamine P1 proteins, with E value 0.0018.
ProDom Summary: Protein Domain Search
Residues 50-120 are 41% similar to a putative nuclear antigen domain, PD043353, as seen in Q69340_VVVVV (Pseudorabies virus).Residues 44-97 are 49% similar to a collagen domain, PD000007, as seen in O44367_MYTED.
Paralogs: Local Blast Search
None.
Pfam Summary: Pfam Search
No significant results.
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular |
Top PDB Hits:
None.
Gene Protein Sequence:
MTASAAATRRRNRSRSARSRAQDPRRARRAAVSQATRWRTCTRGEKHTCG
RGDTGVGGASGGRRGVRGSEGRQGVGGASGGRRGVRGSEGRQGVGGEAYL
PARRVRGRGRGGPPPAQAQARQVLRRGAQP$
Gene Nucleotide Sequence: Sequence Viewer
ATGACCGCCTCGGCCGCCGCCACGCGGCGCCGGAACCGGTCGCGGTCGGC
CCGCTCGCGCGCCCAGGACCCCCGTCGGGCCAGGCGCGCGGCCGTCTCCC
AGGCCACCAGATGGCGCACCTGCACGCGCGGCGAGAAGCACACCTGCGGG
CGGGGAGACACGGGGGTCGGAGGGGCGTCAGGGGGTCGGAGGGGCGTCAG
GGGGTCGGAGGGGCGTCAGGGGGTCGGAGGGGCGTCAGGGGGTCGGAGGG
GCGTCAGGGGGTCGGAGGGGCGTCAGGGGGTCGGAGGGGAGGCGTACCTT
CCCGCGCGGCGCGTCCGCGGGCGGGGACGCGGGGGGCCGCCGCCGGCGCA
GGCTCAGGCGCGCCAGGTACTCCGTCGTGGTGCGCAGCCGTAG