Gene ID:HHVTW003
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
136776
Gene Name:
UL1
Definition:
virion glycoprotein L, gL (VGLL_HSV2H)
Cellular Location:
[Evidence]
Gene Start:
9427
Gene Stop:
10101
Gene Length:
675
Molecular Weight*:
25178
pI*:
10.60
Net Charge*:
10.96
EC:
Functional Class:
protein transport
virion entry and egress; cell membrane penetration
Gene Ontology:
Pathway: pathway table
Primary Evidence:
McGeoch,D.J., Cunningham,C., McIntyre,G. and Dolan, Comparative sequence analysis of the long repeat regions and adjoining parts of the long unique regions in the genomes of herpes simplex viruses types 1 and 2, J. Gen. Virol. 72 (Pt 12), 3057-3075 (1991), Medline: 92113549.
Secondary Evidence:
Westra DF, Kuiperij HB, Welling GW, Scheffer AJ, The TH Welling-Wester S, Domains of glycoprotein H of herpes simplex virus type 1 involved in complex formation with glycoprotein L, Virology 1999 Aug 15;261(1):96-105, Medline: 99373446.
Turner A, Bruun B, Minson T, Browne H, Glycoproteins gB, gD, and gHgL of herpes simplex virus type 1 are necessary and sufficient to mediate membrane fusion in a Cos cell transfection system, J Virol 1998 Jan;72(1):873-5, Medline: 98080492.
Peng T, Ponce-de-Leon M, Jiang H, Dubin G, Lubinski JM, Eisenberg RJ, Cohen GH, The gH-gL complex of herpes simplex virus (HSV) stimulates neutralizing antibody and protects mice against HSV type 1 challenge, J Virol 1998 Jan;72(1):65-72, Medline: 98080390.
Westra DF, Glazenburg KL, Harmsen MC, Tiran A, Jan Scheffer A, Welling GW, Hauw The T, Welling-Wester S, Glycoprotein H of herpes simplex virus type 1 requires glycoprotein L for transport to the surfaces of insect cells, J Virol 1997 Mar;71(3):2285-91,
Medline: 97184562.
Novotny MJ, Parish ML, Spear PG, Variability of herpes simplex virus 1 gL and anti-gL antibodies that inhibit cell fusion but not viral infectivity, Virology 1996 Jul 1;221(1):1-13, Medline: 96266410.
Dubin G, Jiang H, Expression of herpes simplex virus type 1 glycoprotein L (gL) in transfected mammalian cells: evidence that gL is not independently anchored to cell membranes, J Virol 1995 Jul;69(7):4564-8, Medline: 95287521.
Ghiasi H, Kaiwar R, Slanina S, Nesburn AB, Wechsler SL, Expression and characterization of baculovirus expressed herpes simplex virus type 1 glycoprotein L, Arch Virol 1994;138(3-4):199-212, Medline: 95091515.
Hutchinson L, Browne H, Wargent V, Davis-Poynter N, Primorac S,
Goldsmith K, Minson AC, Johnson DC, A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH, J Virol 1992 Apr;66(4):2240-50, Medline: 92194460.
McGeoch DJ, Dalrymple MA, Davison AJ, Dolan A, Frame MC, McNab D
Perry LJ, Scott JE, Taylor P, The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1, J Gen Virol 1988 Jul;69 ( Pt 7):1531-74, Medline: 88274327.
Comment:
Dubin & Jiang(1995) report that gL associates with glycoprotein H (gH) to form a complex which is required for transport of both proteins to the plasma membrane of HSV-1.
Hutchinson et.al. (1992) first suggest that a hetero-oligomer of gH and gL of HSV-1 is incorporated into virions and transported to the cell surface and acts during entry of virus into cells.
UL1 has gamma expression regulation, Roizman, PNAS 93:11307,1996, Medline: 97030190.
Blast Summary: PSI-Blast Search
Residues 1-223 of the UL1 protein are 65% similar using gapped BLAST to residues 1-223 of the UL1 protein in Human herpesvirus 1 (strain 17) and weakly similar (29% over 124 aa) to Cercopithecine herpesvirus 9 (AAD41747), 25% similar over 85 aa to Human herpesvirus 3 (P09308) and smaller segments of other Alphaherpesvirinae.
Variants include AAA45858 (strain 333).
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
None.
ProDom Summary: Protein Domain Search
Residues 1-212 constitute a glycoprotein L precursor domain as seen in VGLL_HSV2H. Residues 66-149 are 27% similar to a glycoprotein L precursor domain as seen in VGLL_HSVEB.
Paralogs: Local Blast Search
None.
Pfam Summary: Pfam Search
None.
Structural Feature(s):
| Feature Type | Start | Stop |
| eukaryotic signal | ||
| non-globular |
Top PDB Hits:
None.
Gene Protein Sequence:
MGFVCLFGLVVMGAWGAWGGSQATEYVLRSVIAKEVGDILRVPCMRTPAD
DVSWRYEAPSVIDYARIDGIFLRYHCPGLDTFLWDRHAQRAYLVNPFLFA
AGFLEDLSHSVFPADTQETTTRRALYKEIRDALGSRKQAVSHAPVRAGCV
NFDYSRTRRCVGRRDLRPANTTSTWEPPVSSDDEASSQSKPLATQPPVLA
LSNAPPRRVSPTRGRRRHTRLRRN$
Gene Nucleotide Sequence: Sequence Viewer
ATGGGGTTCGTCTGTCTGTTTGGGCTTGTCGTTATGGGAGCCTGGGGGGC
GTGGGGTGGGTCACAGGCAACCGAATATGTTCTTCGTAGTGTTATTGCCA
AAGAGGTGGGGGACATACTAAGAGTGCCTTGCATGCGGACCCCCGCGGAC
GATGTTTCTTGGCGCTACGAGGCCCCGTCCGTTATTGACTATGCCCGCAT
AGACGGAATATTTCTTCGCTATCACTGCCCGGGGTTGGACACGTTTTTGT
GGGATAGGCACGCCCAGAGGGCGTATCTGGTTAACCCCTTTCTCTTTGCG
GCGGGATTTTTGGAGGACTTGAGTCACTCTGTGTTTCCGGCCGACACCCA
GGAAACAACGACGCGCCGGGCCCTTTATAAAGAGATACGCGATGCGTTGG
GCAGTCGAAAACAGGCCGTCAGCCACGCACCCGTCAGGGCCGGGTGTGTA
AACTTTGACTACTCACGCACTCGCCGCTGCGTCGGGCGACGCGATTTACG
GCCTGCCAACACCACGTCAACGTGGGAACCGCCTGTGTCGTCGGACGATG
AAGCGAGCTCGCAGTCGAAGCCCCTCGCCACCCAGCCGCCCGTCCTCGCC
CTTTCGAACGCCCCCCCACGGCGGGTCTCCCCGACGCGAGGTCGGCGCCG
GCATACTCGCCTCCGACGCAACTAG