Gene ID:HHVTW008
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
2827770
Gene Name:
UL5
Definition:
component of DNA helicase-primase complex, cellular homolog (HELI_HSV2H)
Cellular Location:
[Evidence]
Gene Start:
15249
Gene Stop:
12604
Gene Length:
2646
Molecular Weight*:
98880
pI*:
6.70
Net Charge*:
-4.18
EC:
Functional Class:
nucleotide synthesis and metabolism; DNA replication
Gene Ontology:
Pathway: pathway table
Primary Evidence:
Holzerlandt R, Orengo C, Kellam P, Alba MM.
Identification of new herpesvirus gene homologs in the human genome,
Genome Res. 2002 Nov;12(11):1739-48,
PMID: 12421761.
McGeoch DJ, Cunningham C, McIntyre G, Dolan A,
Comparative sequence analysis of the long repeat regions and adjoining parts of the long unique regions in the genomes of herpes simplex viruses types 1 and 2,
J Gen Virol 1991 Dec;72 ( Pt 12): 3057-75,
Medline: 92113549.
Secondary Evidence:
Constantin N, Dodson MS,
Two-hybrid analysis of the interaction between the UL52 and UL8 subunits of the herpes simplex virus type 1 helicase-primase,
J Gen Virol 1999 Sep;80 ( Pt 9):2411-5,
Medline: 99429603.
Barnard EC, Brown G, Stow ND,
Deletion mutants of the herpes simplex virus type 1 UL8 protein: effect on DNA synthesis and ability to interact with and influence the intracellular localization of the UL5 and UL52 proteins,
Virology 1997 Oct 13;237(1):97-106,
Medline: 98008983.
Bloom DC, Stevens JG,
Neuron-specific restriction of a herpes simplex virus recombinant maps to the UL5 gene,
J Virol 1994 Jun;68(6):3761-72,
Medline: 94246721.
Zhu LA, Weller SK,
The six conserved helicase motifs of the UL5 gene product, a component of the herpes simplex virus type 1 helicase-primase, are essential for its function,
J Virol 1992 Jan;66(1):469-79,
Medline: 92085418.
Crute JJ, Tsurumi T, Zhu LA, Weller SK, Olivo PD, Challberg MD,
Mocarski ES, Lehman IR,
Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products,
Proc Natl Acad Sci U S A 1989 Apr;86(7):2186-9,
Medline: 89184593.
McGeoch,D.J., Dalrymple,M.A., Davison,A.J., Dolan,A., Frame,M.C., McNab,D., Perry,L.J., Scott,J.E. and Taylor,P.,
The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1,
J. Gen. Virol. 69 (Pt 7), 1531-1574 (1988),
Medline: 88274327.
McGeoch DJ, Dalrymple MA, Dolan A, McNab D, Perry LJ, Taylor P, Challberg MD,
Structures of herpes simplex virus type 1 genes required for replication of virus DNA,
J Virol 1988 Feb;62(2):444-53,
Medline: 88091053.
Comment:
Crute et.al.(1989) report that the UL5 protein of HHV1 complexes with the UL8 and UL52 gene products to form a DNA helicase-primase.
Holzerlandt et al. (2002) identify UL5 as a homolog of human DNA helicase (GI: 5523990).
The UL5 protein belongs to a family that includes HSV-1 UL5, EHV-1 57, EBV BBLF4, HCMV UL105, AND VZV 55.
UL5 has beta expression regulation (Roizman, PNAS 93:11307,1996, Medline: 97030190).
Blast Summary: PSI-Blast Search
Resdiues 1-881 of the UL5 protein are 89% similar using gapped BLAST to residues 1-882 of the UL5 protein in Human herpesvirus 1 (strain 17) and significantly similar, 61% over 855 aa, to Equine herpesvirus 1 (HELI_HSVEB),60% similar over 854 aa to Equine herpesvirus 4 (gene 57), 57% similar over 861 aa to Human herpesvirus 3 (HELI_VZVD), Bovine herpesvirus 1 (CAA88128), many other Alphaherpesvirinae and less similarity with Beta and Gammaherpesvirinae. Variants include WMBEHQ.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
No significant hits.
ProDom Summary: Protein Domain Search
Residues 1-29, 30-558, 559-639 and 640-881 constitute helicase-primase domains as seen in HELI_HSV2H. Residues 1-28 are 65% similar to a probable helicase domain as seen in HELI_HSV11.
Paralogs: Local Blast Search
None.
Pfam Summary: Pfam Search
Residues 92-109 (E value 0.017) may place UL5 in the viral_helicase1 family (PF01443), described as viral (Superfamily 1) RNA helicase.
Residues 122-136 (E value 0.007) may place UL5 in the TIM family (PF00121), described as triosephosphate isomerase. See Prosite PDOC00155.
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular | ||
| non-globular |
Top PDB Hits:
None.
Gene Protein Sequence:
MAASGGEGSRDVRAPGPPPQQPGARPAVRFRDEAFLNFTSMHGVQPIIAR
IRELSQQQLDVTQVPRLQWFRDVAALEVPTGLPLREFPFAAYLITGNAGS
GKSTCVQTLNEVLDCVVTGATRIAAQNMYVKLSGAFLSRPINTIFHEFGF
RGNHVQAQLGQHPYTLASSPASLEDLQRRDLTYYWEVILDITKRALAAHG
GEDARNEFHALTALEQTLGLGQGALTRLASVTHGALPAFTRSNIIVIDEA
GLLGRHLLTTVVYCWWMINALYHTPQYAGRLRPVLVCVGSPTQTASLEST
FEHQKLRCSVRQSENVLTYLICNRTLREYTRLSHSWAIFINNKRCVEHEF
GNLMKVLEYGLPITEEHMQFVDRFVVPESYITNPANLPGWTRLFSSHKEV
SAYMAKLHAYLKVTREGEFVVFTLPVLTFVSVKEFDEYRRLTQQPTLTME
KWITANASRITNYSQSQDQDAGHVRCEVHSKQQLVVARNDITYVLNSQVA
VTARLRKMVFGFDGTFRTFEAVLRDDSFVKTQGETSVEFAYRFLSRLMFG
GLIHFYNFLQRPGLDATQRTLAYGRLGELTAELLSLRRDAAGASATRAAD
TSDRSPGERAFNFKHLGPRDGGPDDFPDDDLDVIFAGLDEQQLDVFYCHY
ALEEPETTAAVHAQFGLLKRAFLGRYLILRELFGEVFESAPFSTYVDNVI
FRGCELLTGSPRGGLMSVALQTDNYTLMGYTYTRVFAFAEELRRRHATAG
VAEFLEESPLPYIVLRDQHGFMSVVNTNISEFVESIDSTELAMAINADYG
ISSKLAMTITRSQGLSLDKVAICFTPGNLRLNSAYVAMSRTTSSEFLHMN
LNPLRERHERDDVISEHILSALRDPNVVIVY$
Gene Nucleotide Sequence: Sequence Viewer
ATGGCGGCGTCCGGCGGGGAGGGCTCGCGGGACGTCCGGGCACCAGGTCC
GCCCCCACAGCAGCCCGGGGCCAGACCCGCCGTGCGGTTCAGGGACGAGG
CGTTTTTAAATTTTACGTCCATGCACGGGGTCCAACCAATCATCGCACGC
ATCCGAGAGCTCTCGCAGCAGCAGCTCGACGTCACGCAGGTGCCGCGCCT
GCAGTGGTTCCGGGACGTGGCGGCCTTGGAGGTCCCGACCGGCCTGCCGC
TCCGGGAGTTTCCGTTCGCGGCGTATCTCATCACCGGCAACGCCGGATCC
GGAAAGAGTACGTGCGTGCAGACCCTCAACGAGGTCCTGGACTGCGTGGT
CACGGGCGCCACGCGAATCGCGGCGCAGAACATGTACGTTAAGCTCTCGG
GGGCGTTTCTGAGTCGACCCATCAACACCATCTTTCACGAGTTCGGGTTT
CGCGGGAATCACGTCCAGGCCCAGCTGGGGCAGCACCCGTACACCCTGGC
CAGCAGCCCCGCCTCGCTGGAAGACCTGCAGCGGCGAGACCTGACGTACT
ACTGGGAGGTGATCCTCGACATCACCAAGCGGGCCCTGGCGGCGCACGGG
GGCGAAGACGCGCGAAACGAGTTCCACGCCCTCACCGCCCTAGAGCAGAC
TTTGGGGCTGGGCCAGGGTGCCCTCACGCGCCTGGCCTCGGTCACACACG
GGGCGCTGCCGGCTTTCACCCGCAGCAACATTATCGTCATCGACGAGGCC
GGGCTCCTGGGGCGGCACCTACTCACGACCGTGGTGTATTGCTGGTGGAT
GATTAACGCCCTGTACCACACCCCCCAGTACGCGGGCCGCCTGCGGCCGG
TGCTGGTGTGCGTGGGGTCGCCGACCCAGACGGCCTCGCTGGAGTCCACC
TTCGAACACCAAAAACTGCGATGCTCCGTCCGGCAGAGCGAAAACGTGCT
CACGTACCTCATCTGCAACCGCACCCTACGCGAGTACACGCGCCTCTCGC
ACAGCTGGGCCATTTTCATTAACAACAAGCGATGTGTGGAGCACGAGTTC
GGGAACCTCATGAAGGTGCTGGAGTACGGCCTTCCCATCACCGAGGAGCA
CATGCAGTTTGTGGACCGCTTTGTCGTCCCGGAAAGTTACATCACCAACC
CGGCCAACCTTCCGGGGTGGACGCGGCTGTTCTCGTCCCACAAGGAGGTC
AGCGCGTACATGGCCAAGCTCCACGCCTACCTAAAGGTGACTCGCGAGGG
GGAGTTTGTTGTGTTTACCCTCCCCGTGCTCACGTTTGTGTCGGTCAAAG
AGTTTGACGAGTATCGACGGCTCACGCAGCAACCCACGCTGACCATGGAA
AAGTGGATCACGGCCAACGCCAGTCGCATCACCAACTACTCCCAGAGTCA
GGACCAGGACGCGGGGCACGTGCGCTGTGAGGTGCACAGCAAGCAACAGC
TAGTCGTGGCCCGGAACGACATCACGTACGTCCTCAACAGCCAGGTCGCG
GTGACCGCGCGCCTCCGAAAGATGGTGTTTGGGTTCGACGGGACGTTTCG
GACCTTCGAGGCTGTGCTGCGCGACGACAGCTTCGTGAAGACCCAGGGGG
AGACCTCGGTGGAGTTCGCCTACCGGTTCCTGTCGCGGCTCATGTTCGGC
GGGCTGATTCACTTTTACAACTTTCTCCAGCGCCCCGGCCTGGACGCGAC
CCAGAGGACCCTGGCCTACGGCCGCCTAGGGGAGCTGACGGCAGAACTCC
TGTCGCTACGCCGGGACGCCGCCGGCGCATCGGCAACCAGGGCCGCCGAC
ACCAGCGACCGCTCTCCGGGGGAGCGTGCGTTCAATTTTAAGCACCTGGG
CCCGCGGGACGGGGGCCCGGACGACTTCCCCGACGACGACCTTGACGTTA
TCTTCGCCGGGCTGGACGAACAGCAGCTGGACGTGTTCTACTGCCACTAC
GCCCTCGAAGAGCCTGAGACCACCGCGGCCGTCCACGCCCAGTTTGGGCT
CCTGAAGAGGGCCTTTCTGGGGCGATACCTTATCCTACGGGAGCTCTTCG
GGGAGGTGTTTGAGAGCGCCCCCTTCAGCACCTACGTGGACAATGTCATT
TTCCGGGGCTGCGAGCTGCTGACCGGCTCGCCGCGCGGGGGGCTGATGTC
CGTGGCCCTGCAGACGGACAACTACACGCTGATGGGGTACACGTACACCC
GGGTGTTCGCGTTCGCGGAGGAGCTGCGGCGGCGGCACGCGACGGCCGGC
GTGGCCGAGTTCTTGGAGGAGTCCCCCCTGCCCTACATCGTCCTGCGGGA
CCAGCACGGCTTCATGTCTGTCGTCAATACCAACATCAGTGAGTTTGTCG
AGTCGATCGACTCCACGGAGCTGGCCATGGCCATCAACGCCGACTACGGC
ATCAGCTCCAAACTCGCGATGACCATCACGCGCTCCCAGGGGCTCAGTCT
GGACAAGGTCGCCATATGCTTCACGCCCGGAAACCTGCGCCTAAACAGCG
CGTACGTAGCCATGTCCCGCACCACCTCATCCGAGTTCCTGCACATGAAT
CTAAACCCGCTCCGGGAGCGCCACGAACGCGATGACGTCATTAGCGAGCA
CATACTATCTGCTCTACGCGATCCGAATGTGGTCATTGTCTATTAA