Gene ID:HHVTW012
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
6572415
Gene Name:
UL9
Definition:
ori-binding protein
Cellular Location:
[Evidence]
Gene Start:
23318
Gene Stop:
20715
Gene Length:
2604
Molecular Weight*:
215436
pI*:
8.30
Net Charge*:
16.94
EC:
Functional Class:
nucleotide synthesis and metabolism; DNA binding
Gene Ontology:
Pathway: pathway table
Secondary Evidence:
Monahan SJ, Grinstead LA, Olivieri W, Parris DS, Interaction between the herpes simplex virus type 1 origin-binding and DNA polymerase accessory proteins, Virology 1998 Feb 1;241(1):122-30,
Medline: 98122997.
Boehmer PE, The herpes simplex virus type-1 single-strand DNA-binding protein, ICP8, increases the processivity of the UL9 protein DNA helicase, J Biol Chem 1998 Jan 30;273(5):2676-83, Medline: 98112811.
Stow ND, Brown G, Cross AM, Abbotts AP, Identification of residues within the herpes simplex virus type 1 origin-binding protein that contribute to sequence-specific DNA binding, Virology 1998 Jan 20;240(2):183-92, Medline: 98118443.
Malik AK, Shao L, Shanley JD, Weller SK, Intracellular localization of the herpes simplex virus type-1 origin binding protein, UL9, Virology 1996 Oct 15;224(2):380-9 (erratum appears in Virology 1997 Jan 6;227(1):268), Medline: 97028486.
Baradaran K, Dabrowski CE, Schaffer PA, Transcriptional analysis of the region of the herpes simplex virus type 1 genome containing the UL8, UL9, and UL10 genes and identification of a novel delayed-early gene product, OBPC, J Virol 1994 Jul;68(7):4251-61, Medline: 94267884.
Martinez R, Edwards CA, Expression, purification, and functional characterization of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein, Protein Expr Purif 1993 Feb;4(1):32-7,
Medline: 93144956.
Boehmer PE, Dodson MS, Lehman IR, The herpes simplex virus type-1 origin binding protein. DNA, helicase activity, J Biol Chem 1993 Jan 15;268(2):1220-5, Medline: 93123237.
Olivo PD, Nelson NJ, Challberg MD, Herpes simplex virus DNA replication: the UL9 gene encodes an origin-binding protein, Proc Natl Acad Sci U S A 1988 Aug;85(15):5414-8, Medline: 88289732.
McGeoch,D.J., Dalrymple,M.A., Dolan,A., McNab,D., Perry,L.J.,
Taylor,P. and Challberg,M.D, Structures of herpes simplex virus type 1 genes required for replication of virus DNA, J. Virol. 62 (2), 444-453 (1988), Medline: 88091053.
Comment:
The UL9 protein binds to the origin of DNA synthesis in sequence specific fashion and carries out helicase and ATPase activities.
UL9 contains a tandem nucleotide repeat at genome coordinates 22419-22444 bp, with period/unit size 12, copy # 2.2, 100% matches and no insert/deletes.
The HSV-2 complete genome sequence was updated in Genbank Dec. 12, 1999, affecting 7 nt (4 aa) in UL9, located at genome coordinates 22739-22748 (UL9 coordinates 571-580).
In HSV-1 it is essential for growth in cell culture and has beta expression regulation (Roizman, PNAS 93:11307-12,1996, Medline: 97030190).
Blast Summary: PSI-Blast Search
Residues 24-867 of UL9 are 89% similar using gapped BLAST to residues 6-851 of UL9 in Human herpesvirus 1 (strain 17) and 52% similar over 839 aa to Equine herpesvirus 1 (gene 53,P28947), 50% similar over 837 aa to Equine herpesvirus 4 (gene 53,AAC59571), 48% similar over 859 aa to Pseudorabies virus (UL9,CAA65906) and significantly similar to many other Alphaherpesvirinae and less similar to several Betaherpesvirinae.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
Residues 97-109 may represent the block PF01443, (Viral (Superfamily 1) RNA helicase) with an E value of 0.7 (8 of 13 matches).
ProDom Summary: Protein Domain Search
Six segments constitute origin binding DNA domains as seen in P89432_VVVVV. Residues 14-64 are 50% similar to the replication origin binding protein domain as seen in OBP_HSVEB.
Paralogs: Local Blast Search
None.
Pfam Summary: Pfam Search
No significant hits.
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular |
Top PDB Hits:
None.
Gene Protein Sequence:
MNVATCTHQTHHAARAPGATSAPGAASGDPLGARRPIGDDECEQYTSSVS
LARMLYGGDLAEWVPRVHPKTTIERQQHGPVTFPDASAPTARCVTVVRAP
MGSGKTTALIRWLGEAIHSPDTSVLVVSCRRSFTQTLATRFAESGLPDFV
TYFSSTNYIMNDRPFHRLIVQVESLHRVGPNLLNNYDVLVTKSLMSTLGQ
LYSPTMQQLGRVDALMLRLLRTCPRIIAMDATANAQLVDFLCSLRGEKNV
HVVIGEYAMPGFSARRCLFLPRLGPEVLQAALRRRGPAGGAPPPDAPPDA
TFFGELEARLAGGDNVCIFSSTVSFAEVVARFCRQFTDRVLLLHSLTPPG
DVTTWGRYRVVIYTTVVTVGLSFDPPHFDSMFAYVKPMNYGPDMVSVYQS
LGRVRTLRKGELLIYMDGSGARSEPVFTPMLLNHVVSASGQWPAQFSQVT
NLLCRRFKGRCDASHADAAQARGSRIYSKFRYKHYFERCTLACLADSLNI
LHMLLTLNCMHVRFWGHDAALTPRNFCLFLRGIHFDALRAQRDLRELRCQ
DPDTSLSAQAAETEEVGLFVEKYLRPDVAPAEVVALMRGLNSLVGRTRFI
YLVLLEACLRVPMAAHSSAIFRRLYDHYATGVIPTINAAGELELVALHPT
LNVAPVWELFRLCSTMAACLQWDSMAGGSGRTFSPEDVLELLNPHYDRYM
QLVFELGHCNVTDGPLLSEDAVKRVADALSGCPPRGSVSETEHALSLFKI
IWGELFGVQLAKSTQTFPGAGRVKNLTKRAIVELLDAHRIDHSACRTHRQ
LYALLMAHKREFAGARFKLRAPAWGRCLRTHASGAQPNTDIILEAALSEL
PTEAWPMMQGAVNFSTL$
Gene Nucleotide Sequence: Sequence Viewer
ATGAACGTCGCCACCTGCACACACCAGACCCACCACGCCGCCCGCGCCCC
GGGCGCGACGTCGGCGCCCGGCGCGGCCTCGGGGGATCCCCTGGGGGCCC
GGCGTCCCATCGGGGATGACGAGTGCGAGCAGTACACGTCGAGTGTATCG
CTGGCGCGGATGCTTTACGGGGGGGATTTGGCCGAGTGGGTTCCCCGGGT
CCACCCGAAAACAACGATCGAGCGGCAGCAACACGGACCCGTCACCTTCC
CGGACGCGAGCGCCCCGACGGCCCGGTGCGTAACTGTGGTCCGCGCGCCA
ATGGGCTCGGGAAAAACTACCGCGCTGATCCGCTGGTTGGGGGAAGCGAT
CCACTCTCCGGACACGAGTGTGCTGGTCGTCTCCTGTCGTCGGAGTTTTA
CTCAGACCCTGGCGACGCGGTTCGCTGAGTCAGGGTTGCCCGACTTCGTC
ACCTACTTCTCATCCACCAATTACATCATGAACGACCGCCCCTTCCACCG
TCTCATCGTGCAGGTGGAAAGTCTTCATCGCGTGGGCCCGAACCTGTTGA
ACAACTACGACGTGCTCGTCCTGGACGAAGTCATGTCGACGTTGGGCCAA
CTGTACTCGCCGACGATGCAGCAGCTGGGCCGCGTCGACGCGTTGATGCT
GCGCCTGCTGCGCACGTGCCCGCGGATCATCGCCATGGACGCCACCGCCA
ACGCGCAGCTGGTGGACTTTCTGTGCAGCCTCCGGGGCGAAAAGAACGTT
CACGTGGTCATCGGGGAGTACGCCATGCCCGGATTTTCGGCGCGCCGTTG
TCTGTTTCTCCCGCGCCTGGGGCCCGAGGTCCTGCAGGCGGCCCTGCGCC
GCCGGGGGCCGGCGGGCGGGGCGCCCCCCCCGGACGCCCCCCCGGACGCC
ACCTTCTTCGGGGAGCTGGAGGCGCGCCTGGCCGGCGGAGATAACGTCTG
CATCTTCTCGTCGACGGTCTCCTTCGCGGAGGTCGTTGCCAGGTTCTGCC
GGCAGTTTACGGACCGCGTGCTGCTGCTCCACTCGCTCACCCCGCCCGGC
GACGTGACCACATGGGGCCGGTACCGGGTGGTCATCTACACAACGGTCGT
GACGGTGGGCCTTAGCTTCGATCCGCCGCACTTTGACAGCATGTTCGCCT
ACGTGAAACCCATGAACTACGGGCCGGACATGGTGTCCGTGTACCAGTCG
CTGGGGCGGGTACGGACTCTCCGCAAGGGGGAGCTGCTGATCTACATGGA
CGGGTCCGGGGCGCGCTCGGAGCCCGTCTTTACGCCCATGCTGCTCAACC
ACGTGGTGAGCGCCAGCGGGCAGTGGCCGGCACAGTTTTCCCAGGTGACG
AACCTGTTGTGCCGCCGGTTCAAAGGGCGCTGCGACGCGTCGCACGCCGA
CGCGGCGCAGGCGCGGGGGTCGCGCATCTACAGCAAATTCCGGTACAAGC
ACTACTTCGAGAGGTGCACGCTGGCGTGCCTCGCGGACAGTCTTAACATC
CTCCACATGCTTCTGACCCTCAACTGCATGCACGTGCGGTTCTGGGGCCA
CGACGCCGCGCTGACCCCGAGGAACTTTTGTCTGTTTTTGCGGGGGATAC
ATTTTGATGCCCTGAGGGCCCAGCGGGATCTGCGGGAGCTGCGCTGCCAG
GACCCCGACACGTCCCTGTCGGCCCAGGCCGCCGAGACGGAGGAGGTGGG
CCTTTTCGTCGAAAAGTACCTCCGGCCGGACGTCGCGCCGGCCGAGGTGG
TCGCGCTCATGCGCGGCCTCAACAGCCTGGTCGGCCGCACGCGGTTCATC
TACCTGGTGCTGCTGGAGGCCTGTCTTCGCGTCCCCATGGCCGCCCATAG
CAGCGCCATCTTCCGGCGGCTTTACGACCACTACGCCACGGGCGTCATCC
CCACGATCAACGCCGCCGGAGAGCTGGAGCTTGTGGCCCTACACCCCACC
CTAAACGTCGCCCCCGTCTGGGAGCTGTTCCGTCTGTGCAGCACCATGGC
CGCGTGCCTGCAGTGGGACTCGATGGCCGGGGGGTCGGGGCGAACCTTTA
GCCCCGAGGACGTGCTGGAGCTGCTGAACCCCCACTACGACCGCTACATG
CAGCTGGTGTTCGAACTGGGCCACTGTAACGTGACCGACGGCCCCTTGCT
GTCGGAGGACGCGGTTAAGCGCGTGGCCGACGCCCTGAGCGGCTGCCCCC
CGCGCGGGTCCGTGAGCGAGACGGAGCACGCGCTGTCGCTGTTCAAGATC
ATCTGGGGCGAACTGTTCGGGGTGCAGCTGGCCAAGAGCACGCAGACGTT
TCCCGGGGCGGGGCGCGTTAAAAACCTCACCAAGCGAGCCATCGTGGAGC
TGCTGGACGCCCACCGCATCGACCACAGCGCCTGCCGGACGCACAGACAG
CTGTACGCGCTGCTGATGGCCCATAAGCGGGAGTTTGCGGGCGCGCGCTT
CAAGCTGCGCGCGCCCGCGTGGGGGCGCTGCTTGCGCACGCACGCCTCCG
GCGCCCAGCCCAACACTGACATCATTCTCGAGGCGGCTCTGTCGGAGCTT
CCCACCGAGGCCTGGCCCATGATGCAGGGGGCGGTGAACTTTAGCACCCT
ATAA