Gene ID:HHVTW017
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
1869837
Gene Name:
UL15
Definition:
DNA cleavage/packaging protein
Cellular Location:
[Evidence]
Gene Start:
28969
Gene Stop:
34772
Gene Length:
5804
Molecular Weight*:
80966
pI*:
8.30
Net Charge*:
10.10
EC:
Functional Class:
virion production; DNA packaging
Gene Ontology:
Pathway: pathway table
Secondary Evidence:
Salmon B, Nalwanga D, Fan Y, Baines JD, Proteolytic cleavage of the amino terminus of the U(L)15 gene product of herpes simplex virus type 1 is coupled with maturation of viral DNA into unit-length genomes, J Virol 1999 Oct;73(10):8338-48, Medline: 99412349.
Koslowski KM, Shaver PR, Casey JT 2nd, Wilson T, Yamanaka G, Sheaffer AK, Tenney DJ, Pederson NE, Physical and functional interactions between the herpes simplex virus UL15 and UL28 DNA cleavage and packaging proteins, J Virol 1999 Feb;73(2):1704-7, Medline: 99099070 .
Yu D, Weller SK, Genetic analysis of the UL 15 gene locus for the putative terminase of herpes simplex virus type 1, Virology 1998 Mar 30;243(1):32, Medline: 98201643.
Baines JD, Cunningham C, Nalwanga D, Davison A, The U(L)15 gene of herpes simplex virus type 1 contains within its second exon a novel open reading frame that is translated in frame with the U(L)15 gene product, J Virol 1997 Apr;71(4):2666-73, Medline: 97213930.
Yu D, Sheaffer AK, Tenney DJ, Weller SK, Characterization of ICP6::lacZ insertion mutants of the UL15 ene of herpes simplex virus type 1 reveals the translation of two proteins, J Virol 1997 Apr;71(4):2656-65, Medline: 97213929.
Baines JD, Poon AP, Rovnak J, Roizman B, The herpes simplex virus 1 UL15 gene encodes two proteins and is required for cleavage of genomic viral DNA, J Virol 1994 Dec;68(12):8118-24. Medline: 95056039.
Poon AP, Roizman B, Characterization of a temperature-sensitive mutant of the UL15 open reading frame of herpes simplex virus 1,
J Virol 1993 Aug;67(8):4497-503, Medline: 3323189.
Dolan A, Arbuckle M, McGeoch DJ, Sequence analysis of the splice junction in the transcript of herpes simplex virus type 1 gene UL15, Virus Res 1991 Jun;20(1):97-104, Medline: 92024477.
McGeoch,D.J., Dalrymple,M.A., Davison,A.J., Dolan,A., Frame,M.C. McNab,D., Perry,L.J., Scott,J.E. and Taylor,P., The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1, J. Gen. Virol. 69 (Pt 7), 1531-1574 (1988), Medline: 88274327.
McGeoch,D.J., Dolan,A. and Frame,M.C., DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes, Nucleic Acids Res. 14 (8), 3435-3448 (1986), Medline: 86205244.
Comment:
UL15 is highly conserved among the Herpesviridae and reported research is with HSV-1. UL15 has two exons with a 3.6 kbase intron. Genes UL16 and UL17 are coded on the opposite strand of the UL15 intron. Also UL15.5 begins at UL15 Met443 and is 3' coterminal and in frame with UL15.
Poon and Roizman (1993) report that the UL15 protein is required for assembly of full DNA-containing capsids and Roisman (PNAS vol. 93, 11307-12, 1996, Medline: 97030190) assigns it gamma expression regulation in HSV-1.
UL15 functions in DNA cleaving and packaging and belongs to a family that includes HSV-1 UL15, HSV-6 ORF12L, EHV-1 44, HCMV UL89, EBV BGRF1/BDRF1, and VZV 42/45.
Blast Summary: PSI-Blast Search
Residues 1-734 of the UL15 protein are 94% similar using gapped BLAST to residues 1-735 of the UL15 protein in Human herpesvirus 1 (strain 17) and 64% similar over 733 aa to Equine herpesvirus 4 (gene 47/44,AAC59564) and 64% similar over 727 aa to Equine herpesvirus 1 and also significantly similar to human herpesvirus 3 (gene 42), bovine herpesvirus 1 (UL15) other Alphaherpesvirinae and less similar to Beta and Gammaherpesvirinae.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
None.
ProDom Summary: Protein Domain Search
Residues 1-60, 61-343, 346-372 and 375-730 constitute DNA packaging protein domains as seen in P89438_VVVVV. Residues 134-193 are 50% similar to a probable packaging protein domain as seen in VTER_HSVEB. Residues 61-130 are 95% similar to a probable packaging protein domain as seen in VTER_HSV11.
Paralogs: Local Blast Search
None.
Pfam Summary: Pfam Search
None.
Structural Feature(s):
| Feature Type | Start | Stop |
| coil-coil |
Top PDB Hits:
None.
Gene Protein Sequence:
MFGQQLASDVQQYLERLEKQRQQKVGVDEASAGLTLGGDALRVPFLDFAT
ATPKRHQTVVPGVGTLHDCCEHSPLFSAVARRLLFNSLVPAQLRGRDFGG
DHTAKLEFLAPELVRAVARLRFRECAPEDAVPQRNAYYSVLNTFQALHRS
EAFRQLVHFVRDFAQLLKTSFRASSLAETTGPPKKRAKVDVATHGQTYGT
LELFQKMILMHATYFLAAVLLGDHAEQVNTFLRLVFEIPLFSDTAVRHFR
QRATVFLVPRRHGKTWFLVPLIALSLASFRGIKIGYTAHIRKATEPVFDE
IDACLRGWFGSSRVDHVKGETISFSFPDGSRSTIVFASSHNTNGIRGQDF
NLLFVDEANFIRPDAVQTIMGFLNQANCKIIFVSSTNTGKASTSFLYNLR
GAADELLNVVTYICDDHMPRVVTHTNATACSCYILNKPVFITMDGAVRRT
ADLFLPDSFMQEIIGGQARETGDDRPVLTKSAGERFLLYRPSTTTNSGLM
APELYVYVDPAFTANTRASGTGIAVVGRYRDDFIIFALEHFFLRALTGSA
PADIARCVVHSLAQVLALHPGAFRSVRVAVEGNSSQDSAVAIATHVHTEM
HRILASAGANGPGPELLFYHCEPPGGAVLYPFFLLNKQKTPAFEYFIKKF
NSGGVMASQELVSVTVRLQTDPVEYLSEQLNNLIETVSPNTDVRMYSGKR
NGAADDLMVAVIMAIYLAAPTGIPPAFFPITRTS
Gene Nucleotide Sequence: Sequence Viewer
ATGTTTGGCCAGCAGCTGGCGTCCGACGTGCAGCAGTACCTGGAGCGCCT
GGAGAAACAGAGGCAACAGAAGGTGGGCGTCGACGAGGCGTCGGCGGGCC
TGACGCTCGGCGGCGATGCGCTGCGCGTCCCTTTTTTGGATTTTGCCACC
GCGACGCCCAAGCGCCACCAGACCGTGGTCCCGGGCGTCGGGACGCTCCA
CGACTGCTGCGAGCACTCGCCGCTCTTCTCGGCCGTCGCGCGGCGGTTGC
TGTTTAATAGCCTGGTGCCGGCGCAACTCAGGGGGCGTGACTTTGGGGGC
GACCACACGGCCAAGCTGGAGTTCCTGGCCCCCGAGCTGGTGCGGGCGGT
GGCGCGCCTGCGGTTTCGGGAGTGCGCGCCGGAGGACGCCGTGCCCCAAC
GCAACGCCTACTACAGCGTCCTGAACACGTTTCAGGCCCTGCACCGCTCC
GAAGCCTTTCGGCAGTTGGTTCACTTCGTGCGGGACTTCGCCCAGTTGTT
GAAAACCTCGTTCCGGGCCTCTAGTCTCGCGGAGACTACGGGCCCCCCGA
AGAAACGGGCCAAGGTGGACGTGGCCACCCACGGGCAGACGTACGGCACC
TTGGAGCTCTTCCAGAAAATGATACTAATGCACGCGACCTACTTTCTGGC
CGCCGTGCTGCTCGGGGACCACGCGGAGCAGGTCAACACGTTCCTGCGGC
TCGTGTTCGAGATCCCCCTGTTTAGCGACACGGCCGTGCGGCACTTCCGC
CAGCGCGCCACCGTGTTTCTAGTCCCCAGGCGCCACGGAAAGACCTGGTT
TTTGGTGCCCCTCATCGCGCTGTCGCTCGCGTCCTTCCGGGGGATCAAGA
TAGGCTACACGGCCCACATCCGCAAGGCGACCGAGCCCGTGTTTGATGAG
ATCGACGCCTGCCTGCGGGGCTGGTTTGGCTCGTCCCGGGTGGACCACGT
CAAGGGGGAAACCATCTCGTTCTCGTTCCCGGACGGCTCGCGCAGCACGA
TCGTGTTTGCCTCCAGCCACAACACGAACGTAAGTACGCCTTCCTCCCGC
GGTGCCTGTTTCCCCGGTGCCGCCCTCCCCGAGATCGACCGACAGACAAA
CACAGCCAGACGCGAGTGTGGGACGACACGCCCGCAGCCCCCCCCCCGCC
ATGGCGGGGGGGAAGCCTTACTGTTTATTTGTAATCGGACGATGAGGCTC
TGGCCACGGCCCGCGCGACCGCGGGGCAGCTCGTTGCAAACAGGCGGCTG
GTATACGATGACAGAACGCAGAGGCGCCACCCGGCGCTGGTCGGGCGGAT
GACGCTTTCCGCGCCGTCCCGGCCCACGACGACCTCGTGCAGGTGGGCCG
TGATGCGCGGGCGGCGGGTCGCCTGCCGCAGGATAACCGCGTCCACGGGG
TGCCCGAAGAGGAGCTGACACAGGCTCGCGTCCCCCCGGACGGCCAGGGT
GCGCTGGGCCATATTGGACCACATGCACGGGGCGACGCAGGGACAGGCCT
CCGCCACGGCGGGGGCGCGCCACAGCGCGTTGGCGGAATCGATGTGGGCC
GTCGGGGCGCAGGCGCCGCCTCCTCCCGGGGGGTCGGTAATCCTGGATAG
CAGCCATCCTAAATGGCGGGCCCGGCTGCCCGGGGGACAGAGCGACCCCA
GGTCATCATCCATGGCCCAGCAGTATATGCGGCCGCCGGGGAGGTGCCAC
CAGGCCCCCGGACCCAGGGCACAGCACGCCCCGGATTCGGGGGCCGTGTC
CGTGGGTACCAGGTAGGCGCCGTCGAGCTCGTGGGCCACGGGCTCGTCCG
CGAGCTGTTCGGCGGCGGGGTCGGGGGTTTCCTCCGGGGGGGAGGCAGCT
TCCAGGTGGCCGAAGGCTAGGGTGCACAGCAGCGGGGTCCGGGGGTGCGT
TACGCTGCGGAGGTGGACGGTGGCGCAGTAGCGGCGCTCGCGGTTAAAGA
AGAAAATGGCAAAGAACGTGTTCGAAGGCAGGCGCAGCGCCTTGGGCCGC
GTCAGGTACAGGAAGATCTCGCAGAAAAGGGCACGCTCGGGGTCGGGGTC
CGGAAGGGCCACCTGGCACAGCGGCTCGGTGAGGACCGTGAGGCACCGAA
AAATCTTAAGCCGCTCGTCCCCCCGAACGACGCGCCACACGAAGACAGAG
TTGGCGATGCGCGCGACGAGGTCGGCTTCGGGCCCCGGGTCGGGGGCGCG
CGCGTCGGGGGGGGCGCCCCGGTGACCCGGCGGGGCCGCGGCTCCCGGGG
GGCCTGGCGTCGCCTGGGGACGCCAGAGTGCCCGCTGTGCCAGGTTGGTG
GTGGGGAAGGGACCGGAGACGCACCAAAAGCAGAGGGGCCAGCGCGTGTA
TGAGTTGGGGGGGGGGTGGGTGAGCGGTGGAACAAAAGCACGCGTCAGCG
GACAAGGCCGGGTCCCGTAGCCGCCCCGCGACAGAACCGGAGTCCGACGG
CACGCGCGACGGGGTCTGCGAGGCTGAGGTACGCCGCGGTGTTAATGGTA
AACGCAAAGCCTCCCGGAAAGACCACTAGCCCGCAGAGGCGGCGATTGAA
CCCAAGGCAGAGGTACGCGTAGCTCTCTCCCGGAAGGTATTGCTCGCAGA
CCCTGTGTGGGGCAGTGGAGGGGCTGCCCTCCATGAAGCGACATTTACTC
TGCTCGCGTCCATTGACGTCACCGTCAATCACCACTGCGATTGGACGGTT
GGTGAGGCGCAGCGTGTCTCCGCTGGTGCTGTAGTAGTCAAACGCGTAGT
GGGCGTCGGAGTCGGCGAAGCGGGCGGGGATGTCGTCGCTGAGAGGGACG
AGCCGCCGCCGCCGCCCCCGACCGCCCTGGCCGCCCAGATGCGCCAGCAC
GGCCAGGGCGTACGCGGTGTGAAAGAACGCGTCGGGGGCGGTCCCCTCGA
GGGCGCGCATCAGGTTCTCCAGGAGCACGGGGAAGCGCCGCGTCACCTCC
CCTAGCCACTCGCTCTGGTGGGGGCCAAAGTCGTAGCGCAGGCGCTGGAA
GATGCGCGGGCCGCCTTGGAGCGCGGCCCGGATAGAGTGGCCCAGGGCCC
GCAGACACGCGATCTGGATGCGCGCGACGAAGGCCACCTCGGCCGCGATG
TCAAAGGGCTGCAGCACGGGGCGCGGGTGGCGCAGGGGTCCCTCGAGCGC
GGGAAAGCGACGCAGCAGCGCCGTCTGGGCCGCGGGGGACAGCTGGTGGG
GGCGCACGACGCGCTCGGCGGCACAGGCCTCCGTCAGGGCCGTGGCCAGC
TCGGAGGACAGCCGCGGGGGGCGGGCGCGTCGCCCGCCCCACGCCACCGA
ATTCTCGTAGGAGACGACGACGAAGCGCTGCTTGGTCCCGTAGTGATGGC
GCAGGACCACGGAGATGGAGCGACGGCTCCACAGCCAGTCGGGCCGGTCG
CCGCCGGCCAGAGCTTCCCACCCGCGGTCCAGCCACTCGACCAGCGATCG
CGGCTTGGCGGTCCCCGGCACGAGGGTGAGCACGTCGTTGAGGACGTCCT
CGCCCGCGGCCCGGGGGCCCCCCCGGCTGGCAAAGCGCCCCCCGCCGGGC
GGCTCCAGGCCCGCCAGCACCGCCTCCGCGTCCGACGCGCCCAGGGCTCC
CCCGCTGACGGCCTGGTGGACCAGGGCGCCCTGGCGGAGCCCCGAGGCGA
CGCCGGAGGCCGCGTGCTTGGGGCGCGCGCGGACCGGGTGGCGGCGGGTG
ACGTCCTGCACGGCCCGCTGGACCAGCGCGAGGATCTCCTCGTTCTCTTG
CGTGATGGACACGTCCTCCGCGGTGGCCGTGTCGCCTCCCGGGGCCGTGA
GCTGCTCCTCCGGGGAGATGGGGGGGTCTGGGGTGCCGACAACGGCCGGC
CCGGCCCCGCCCGAGACCGAGGACGCCTGGGGAGTGGGGGTGCCGCTTTC
CCCCATCCCCAGGGACAGGTGGGCCGCCGCCTCCGTCGCGGCGGCGGGAG
CCGCGGCCCCCAGCCGCGCGACGTAGCGACAAAAGTGGCGACAGAGGCGC
ATGAGGCGCGCGCCGTCGGCCGCGTATCGCGTGTTTGGCGGGACGAGCTC
GTCGTAACTGAACAGGAGCACGCGGGCACAGGTCGCCCACGGGCCCCACG
CCAGGCGCAGCGCCGCGACCGTGTACGGGTCGTACACGCCTTGGGCGTCG
CACGCGACCGGCAGGGAGACGAACAGCCCGCCCGCGCTGGGGACGCGCGG
CAGGAGGTCCGGGTGCGCCGGGATGACGGGGGCTAGGATCGCCCCCACCG
CATCCGCCGGCACGTAGGCGGCAAACGCCGAACGCCACGGGGTGCAGTCG
CCGGTCGCGTGGGCCCGGGTCTGGGTTTCGACCCGGAAGTTCGCGGCCGC
CCCACCGTCGGGGCGGCCGCGCACGAGGGCGGACAGCGGGACCCCCGCCG
CCGCCAGGCACTCGCTGGAGATGATGACGTGAATCAGCGAGGCGGGGCTG
CTCGGGTCCCGGGTGAGATCGTATTGGACCTCGTTGGCAAAGTGCGCGTT
CATGGCCCGGCCGGCGGTGCGAGCCCTTCCCGGTGCCGGAAGGGGCGTGG
GTGGGGGGTGCGTGTGCGCGTCCTCGGGGCCCGCGGGCGCACGTGCGCTT
ATACGCTGTGTGTTTCGTCTGTCCCCAGGGAATCCGGGGCCAGGACTTTA
ACCTGCTTTTCGTCGACGAGGCCAACTTTATTCGCCCGGATGCGGTCCAG
ACGATTATGGGCTTTCTCAATCAGGCCAACTGCAAGATCATCTTCGTCTC
GTCGACCAACACCGGGAAGGCCAGCACGAGCTTTTTGTACAACCTCCGCG
GGGCCGCCGACGAGCTGCTCAACGTGGTCACCTATATATGCGACGACCAC
ATGCCGCGGGTGGTGACGCACACCAACGCCACGGCCTGTTCCTGCTATAT
CCTGAACAAACCCGTGTTTATCACGATGGACGGCGCCGTTCGCCGGACGG
CCGATCTGTTTCTGCCCGACTCCTTCATGCAGGAGATCATCGGGGGGCAG
GCCCGCGAGACCGGCGACGACCGGCCCGTCCTAACAAAGTCGGCGGGGGA
GCGGTTTCTGCTGTACCGCCCCTCCACCACCACCAACAGCGGCCTGATGG
CCCCCGAGCTGTACGTGTACGTGGACCCGGCGTTCACGGCCAACACGCGC
GCCTCCGGCACCGGCATCGCGGTCGTCGGGAGGTACCGCGACGATTTCAT
TATCTTCGCCCTGGAGCACTTTTTCCTCCGCGCGCTCACGGGATCGGCCC
CCGCGGACATCGCCCGCTGCGTCGTGCACAGCCTCGCCCAGGTGCTGGCG
CTGCACCCCGGGGCGTTTCGCAGCGTTCGCGTGGCGGTCGAGGGCAACAG
CAGCCAGGACTCGGCCGTGGCCATCGCCACACACGTGCATACCGAGATGC
ACCGCATCCTGGCCTCGGCGGGGGCCAACGGCCCGGGGCCCGAGCTCCTC
TTCTATCACTGCGAGCCGCCCGGCGGCGCGGTATTGTACCCCTTCTTTCT
GCTCAACAAACAGAAGACGCCCGCCTTCGAATACTTTATCAAAAAGTTCA
ACTCCGGGGGCGTCATGGCGTCCCAGGAGCTCGTCTCCGTGACGGTGCGC
CTGCAGACCGACCCGGTCGAGTATCTGTCCGAGCAGCTCAACAACCTCAT
CGAAACCGTCTCTCCCAACACCGACGTCCGCATGTACTCCGGAAAACGCA
ACGGTGCCGCGGACGACCTCATGGTCGCGGTCATCATGGCCATTTACCTG
GCGGCCCCGACCGGGATCCCCCCGGCCTTTTTTCCGATCACGCGCACGTC
TTGA