Gene ID:HHVTW028
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
1869848
Gene Name:
UL26
Definition:
serine protease, self-cleaves to form VP21 and VP24
Cellular Location:
[Evidence]
Gene Start:
51029
Gene Stop:
52942
Gene Length:
1914
Molecular Weight*:
66932
pI*:
7.10
Net Charge*:
1.34
EC:
Functional Class:
protein processing; protein cleavage
virion production; capsid scaffold
Gene Ontology:
Pathway: pathway table
Primary Evidence:
Steffy KR, Schoen S, Chen CM , Nucleotide sequence of the herpes simplex virus type 2 gene encoding the protease and capsid protein ICP35, J Gen Virol 1995 Apr;76 ( Pt 4):1069-72, Medline: 97201574.
Secondary Evidence:
Person S, Desai P , Capsids are formed in a mutant virus blocked at the maturation site of the UL26 and UL26.5 open reading frames of herpes simplex virus type 1 but are not formed in a null mutant of UL38 (VP19C), Virology 1998 Mar 1;242(1):193-203, Medline: 98162581.
Hann LE, Cook WJ, Uprichard SL, Knipe DM, Coen DM , The role of herpes simplex virus ICP27 in the regulation of UL24 gene expression by differential polyadenylation, J Virol 1998 Oct;72(10):7709-14, Medline: 98406170.
Preston VG, al-Kobaisi MF, McDougall IM, Rixon FJ, The herpes simplex virus gene UL26 proteinase in the presence of the UL26.5 gene product promotes the formation of scaffold-like structures, J Gen Virol 1994 Sep;75 ( Pt 9):2355-66, Medline: 94358735.
Shieh HS, Kurumbail RG, Stevens AM, Stegeman RA, Sturman EJ, Pak JY, Wittwer AJ, Palmier MO, Wiegand RC, Holwerda BC, Stallings WC, Three-dimensional structure of human cytomegalovirus protease, Nature 1996;383:279-282, Medline: 96399137.
Davison MD, Rixon FJ, Davison AJ , Identification of genes encoding two capsid proteins (VP24 and VP26) of herpes simplex virus type 1, J Gen Virol 1992 Oct;73 ( Pt 10):2709-13, Medline: 93019027.
Person S, Laquerre S, Desai P, Hempel J , Herpes simplex virus type 1 capsid protein, VP21,originates within the UL26 open reading frame, J Gen Virol 1993 Oct;74 ( Pt 10):2269-73, Medline: 94015013.
Liu F, Roizman B, Differentiation of multiple domains in the herpes simplex virus 1 protease encoded by the UL26 gene, Proc Natl Acad Sci U S A 1992 Mar 15;89(6):2076-80, Medline: 92196060.
Preston VG, Rixon FJ, McDougall IM, McGregor M, al Kobaisi MF, Processing of the herpes simplex virus assembly protein ICP35 near its carboxy terminal end requires the product of the whole of the UL26 reading frame, Virology 1992 Jan;186(1):87-98, Medline: 92087489.
Liu FY, Roizman B, The herpes simplex virus 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate, J Virol 1991 Oct;65(10):5149-56, Medline: 91374562.
Liu FY, Roizman B, The promoter, transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame, J Virol 1991 Jan;65(1):206-12, Medline: 91087283.
Comment:
The UL26 protein is a serine protease, see Liu and Roizmann (1992) and it's substrates are itself and the UL26.5 protein. UL26 cleaves itself between Ala 247 and Ser 248 and also between Ala 612 and Ser 613. Likewise it cleaves off the last 25 residues of the UL26.5 protein. Person et.al.(1993) report that VP21 (aa 248 to 637) and VP24 (aa 1-247) are products of the self cleavage of UL26.
Both the HSV-1 and HSV-2 UL26.5 gene promoter, transcriptional unit and CDS are within, in-frame and 3' coterminal with UL26. In HSV-1 Roizman assigns the UL26 gene gamma expression regulation, (PNAS 93:11307, 1996, Medline: 97030190.
Blast Summary: PSI-Blast Search
Residues 1-637 of the UL26 protein are 73% similar using gapped BLAST to residues 1-635 of the UL26 protein in Human herpesvirus 1 (strain 17) and residues 309-637 are 67% similar to residues 1-329 of the UL26.5 protein in Human herpesvirus 1. UL26 is significantly similar to the UL26 homologs in other Alphaherpesvirinae.
Variants include AAA92139 and AAA67703.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
Residues 20-31, 35-43, 60-69, 70-86, 117-132, 147-156, 162-179, and 237-248 are members of blocks PR00236A-H, described as Herpesvirus capsid protein P40 signature.
ProDom Summary: Protein Domain Search
Residues 1-36, 37-350, 351-390, 391-564 and 596-637 constitute UL26 protease domains as seen in P89449_VVVVV. Residues 502-608 are 39% similar to a high proline domain as seen in SP62_MOUSE.
Paralogs: Local Blast Search
The last 987 bp of UL26 are the gene UL26.5. The two are 3' coterminal at bp 52940.
Pfam Summary: Pfam Search
Residues 37-371 make up the Peptidase_S21 model (PF00716), described as Assemblin (Peptidase family S21).
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular |
Top PDB Hits:
Round 1 hit to pdb|1AT3|1AT3-A Herpes simplex virus type II protease, score 450.0, E value 0e+00.
Round 1 hit to pdb|1VZV|1VZV Structure of Varicella-Zoster virus protease, Score 211.0, E value 1e-53.
Gene Protein Sequence:
MASAEMRERLEAPLPDRAVPIYVAGFLALYDSGDPGELALDPDTVRAALP
PENPLPINVDHRARCEVGRVLAVVNDPRGPFFVGLIACVQLERVLETAAS
AAIFERRGPALSREERLLYLITNYLPSVSLSTKRRGDEVPPDRTLFAHVA
LCAIGRRLGTIVTYDTSLDAAIAPFRHLDPATREGVRREAAEAELALAGR
TWAPGVEALTHTLLSTAVNNMMLRDRWSLVAERRRQAGIAGHTYLQASEK
FKIWGAESAPAPERGYKTGAPGAMDTSPAASVPAPQVAVRARQVASSSSS
SSFPAPADMNPVSASGAPAPPPPGDGSYLWIPASHYNQLVTGQSAPRHPP
LTACGLPAAGTVAYGHPGAGPSPHYPPPPAHPYPGMLFAGPSPLEAQIAA
LVGAIAADRQAGGLPAAAGDHGIRGSAKRRRHEVEQPEYDCGRDEPDRDF
PYYPGEARPEPRPVDSRRAARQASGPHETITALVGAVTSLQQELAHMRAR
THAPYGPYPPVGPYHHPHADTETPAQPPRYPAKAVYLPPPHIAPPGPPLS
GAVPPPSYPPVAVTPGPAPPLHQPSPAHAHPPPPPPGPTPPPAASLPQPE
APGAEAGALVNASSAAHVNVDTARAADLFVSQMMGSR$
Gene Nucleotide Sequence: Sequence Viewer
ATGGCGTCGGCGGAAATGCGCGAGCGGTTGGAGGCGCCTCTGCCCGACCG
GGCGGTGCCCATCTACGTGGCCGGGTTTTTGGCCCTGTACGACAGCGGGG
ACCCGGGCGAGCTGGCCCTGGACCCAGACACGGTGCGTGCGGCCCTGCCT
CCGGAGAACCCCCTGCCGATCAACGTAGACCACCGCGCTCGGTGCGAGGT
GGGCCGGGTGCTCGCCGTGGTCAACGACCCTCGGGGGCCGTTTTTTGTGG
GGCTGATCGCGTGCGTGCAGCTGGAGCGCGTCCTCGAGACGGCCGCCAGC
GCCGCTATTTTTGAGCGCCGCGGACCCGCGCTCTCCCGGGAGGAGCGTCT
GCTGTACCTGATCACCAACTACCTGCCATCGGTCTCGCTGTCCACAAAAC
GCCGGGGGGACGAGGTTCCGCCCGACCGCACCCTGTTTGCGCACGTGGCC
CTGTGCGCCATCGGGCGGCGCCTTGGAACCATCGTCACCTACGACACCAG
CCTAGACGCGGCCATCGCTCCGTTTCGCCACCTGGACCCGGCGACGCGCG
AGGGGGTGCGACGCGAGGCCGCCGAGGCCGAGCTCGCGCTGGCCGGGCGC
ACCTGGGCCCCCGGCGTGGAGGCGCTCACACACACGCTGCTCTCCACCGC
CGTCAACAACATGATGCTGCGTGACCGCTGGAGCCTTGTGGCCGAGCGGC
GGCGGCAGGCCGGGATCGCCGGACACACGTACCTTCAGGCGAGCGAAAAA
TTTAAAATATGGGGGGCGGAGTCTGCCCCTGCGCCGGAGCGCGGGTATAA
AACCGGCGCCCCGGGTGCCATGGACACATCCCCCGCCGCGAGCGTTCCCG
CGCCGCAGGTCGCCGTCCGTGCGCGTCAAGTCGCGTCGTCGTCTTCTTCT
TCTTCTTTTCCGGCACCGGCCGATATGAACCCCGTTTCGGCATCGGGCGC
CCCGGCCCCTCCGCCGCCCGGCGACGGGAGTTATTTGTGGATCCCCGCCT
CTCATTACAATCAGCTCGTCACCGGGCAATCCGCGCCCCGCCACCCGCCG
CTGACCGCGTGCGGCCTGCCGGCCGCGGGGACGGTGGCCTACGGACACCC
CGGCGCCGGCCCGTCCCCGCACTACCCGCCTCCTCCCGCCCACCCGTACC
CGGGTATGCTGTTCGCGGGCCCCAGTCCCCTGGAGGCCCAGATCGCCGCG
CTGGTGGGGGCCATCGCCGCCGACCGCCAGGCGGGTGGGCTTCCGGCGGC
CGCCGGAGACCACGGGATCCGGGGGTCGGCGAAGCGCCGCCGACACGAGG
TGGAGCAGCCGGAGTACGACTGCGGCCGTGACGAGCCGGACCGGGACTTC
CCGTATTACCCGGGCGAGGCCCGCCCCGAGCCGCGCCCGGTCGACTCCCG
GCGCGCCGCGCGCCAGGCTTCCGGGCCCCACGAAACCATCACGGCGCTGG
TGGGGGCGGTGACGTCCCTGCAGCAGGAACTGGCGCACATGCGCGCGCGT
ACCCACGCCCCCTACGGGCCGTATCCGCCGGTGGGGCCCTACCACCACCC
CCACGCAGACACGGAGACCCCCGCCCAACCACCCCGCTACCCCGCCAAGG
CCGTCTATCTGCCGCCGCCGCACATCGCCCCCCCGGGGCCTCCTCTATCC
GGGGCGGTCCCCCCACCCTCGTATCCCCCAGTTGCGGTTACCCCCGGTCC
CGCTCCCCCGCTACATCAGCCCTCCCCCGCACACGCCCACCCCCCTCCGC
CGCCGCCGGGACCCACGCCTCCCCCCGCCGCGAGCTTACCCCAACCCGAG
GCGCCCGGCGCGGAGGCCGGCGCCTTAGTTAACGCCAGCAGCGCGGCCCA
CGTGAACGTGGACACGGCCCGGGCCGCCGATCTGTTTGTGTCACAGATGA
TGGGGTCCCGCTAA