Gene ID:HHVTW029
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
1869849
Gene Name:
UL26.5
Definition:
capsid scaffolding protein, ICP35, VP22a
Cellular Location:
[Evidence]
Gene Start:
51953
Gene Stop:
52942
Gene Length:
990
Molecular Weight*:
33823
pI*:
7.20
Net Charge*:
1.23
EC:
Functional Class:
virion production; capsid scaffold
Gene Ontology:
Pathway: pathway table
Secondary Evidence:
Oien NL, Thomsen DR, Wathen MW, Newcomb WW, Brown JC, Homa FL, Assembly of herpes simplex virus capsids using the human cytomegalovirus scaffold protein: critical role of the C terminus,
J Virol 1997 Feb;71(2):1281-91, Medline: 97151116.
Thomsen DR, Newcomb WW, Brown JC, Homa FL, Assembly of the herpes simplex virus capsid: requiremet or the carboxyl-terminal twenty-five amino acids of the proteins encoded by the UL26 and UL26.5 genes, J Virol 1995 Jun;69(6):3690-703, Medline: 95264461.
Thomsen DR, Roof LL, Homa FL, Assembly of herpes simplex virus (HSV) intermediate capsids in insect cells infected with recombinant baculoviruses expressing HSV capsid proteins, J Virol 1994 Apr;68(4):2442-57, Medline: 94187086.
Matusick-Kumar L, Hurlburt W, Weinheimer SP, Newcomb WW, Brown JC, Gao M, Phenotype of the herpes simplex virus type 1 protease substrate ICP35 mutant virus, J Virol 1994 Sep;68(9):5384-94, Medline: 94335050.
Liu F, Roizman B, Characterization of the protease and other products of amino-terminus-proximal cleavage of the herpes simplex virus 1 UL26 protein, J Virol 1993 Mar;67(3):1300-9Medline: 93172346.
Liu FY, Roizman B, The herpes simplex virus 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate, J Virol 1991 Oct;65(10):5149-56, Medline: 91374562.
Liu FY, Roizman B, The promoter, transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the UL26 open reading frame, J Virol 1991 Jan;65(1):206-12, J Virol 1991 Jan;65(1):206-12, Medline: 91087283.
Comment:
In both the HSV-1 and HSV-2 genomes the UL26.5 gene promoter, transcriptional unit and CDS are within, in-frame and 3' coterminal with UL26. The serine protease of UL26 cleaves UL26.5 between Ala 304 and Ser 305 processing ICP35b,c to ICP35e,f. UL26.5 forms an inner core or capsid scaffolding. Upon packaging of the DNA it is removed from the capsid shell. In HSV-1 the UL26.5 gene is essential and has gamma expression regulation (Roizman, PNAS 93: 11307, 1996, Medline: 97030190).
Blast Summary: PSI-Blast Search
Residues 1-329 of the UL26.5 protein are 67% similar using gapped BLAST to residues 1-329 of UL 26.5 and residues 307-635 of UL26 in Human herpesvirus 1 (strain 17) and weakly similar (29% over 186 aa)to Equine herpesvirus 1 & 4 (AAB02471 & AAC59552) and 27% similar over 180 aa to Bovine herpesvirus 1 (AAA92000) and less similar to other Alphaherpesvirinae.
Variants include: AAA92139 and AAA67703.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
Twelve small (33aa) segments of UL26.5 match the block DM00215, described as proline-rich protein 3 with a combined E value of 4.3e-08.
ProDom Summary: Protein Domain Search
Residues 1-42, 43-82, 83-256 and 288-329 constitute UL26 domains as seen in P90341_VVVVV and P89449_VVVVV. Residues 38-290 are 29% similar to a proline-rich hypothetical protein region as seen in O41935_VVVVV.
Paralogs: Local Blast Search
The last 987 bp of UL26 are the gene UL26.5. The two are 3' coterminal at the stop codon at bp 52940.
Pfam Summary: Pfam Search
None.
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular |
Top PDB Hits:
None.
Gene Protein Sequence:
MNPVSASGAPAPPPPGDGSYLWIPASHYNQLVTGQSAPRHPPLTACGLPA
AGTVAYGHPGAGPSPHYPPPPAHPYPGMLFAGPSPLEAQIAALVGAIAAD
RQAGGLPAAAGDHGIRGSAKRRRHEVEQPEYDCGRDEPDRDFPYYPGEAR
PEPRPVDSRRAARQASGPHETITALVGAVTSLQQELAHMRARTHAPYGPY
PPVGPYHHPHADTETPAQPPRYPAKAVYLPPPHIAPPGPPLSGAVPPPSY
PPVAVTPGPAPPLHQPSPAHAHPPPPPPGPTPPPAASLPQPEAPGAEAGA
LVNASSAAHVNVDTARAADLFVSQMMGSR$
Gene Nucleotide Sequence: Sequence Viewer
ATGAACCCCGTTTCGGCATCGGGCGCCCCGGCCCCTCCGCCGCCCGGCGA
CGGGAGTTATTTGTGGATCCCCGCCTCTCATTACAATCAGCTCGTCACCG
GGCAATCCGCGCCCCGCCACCCGCCGCTGACCGCGTGCGGCCTGCCGGCC
GCGGGGACGGTGGCCTACGGACACCCCGGCGCCGGCCCGTCCCCGCACTA
CCCGCCTCCTCCCGCCCACCCGTACCCGGGTATGCTGTTCGCGGGCCCCA
GTCCCCTGGAGGCCCAGATCGCCGCGCTGGTGGGGGCCATCGCCGCCGAC
CGCCAGGCGGGTGGGCTTCCGGCGGCCGCCGGAGACCACGGGATCCGGGG
GTCGGCGAAGCGCCGCCGACACGAGGTGGAGCAGCCGGAGTACGACTGCG
GCCGTGACGAGCCGGACCGGGACTTCCCGTATTACCCGGGCGAGGCCCGC
CCCGAGCCGCGCCCGGTCGACTCCCGGCGCGCCGCGCGCCAGGCTTCCGG
GCCCCACGAAACCATCACGGCGCTGGTGGGGGCGGTGACGTCCCTGCAGC
AGGAACTGGCGCACATGCGCGCGCGTACCCACGCCCCCTACGGGCCGTAT
CCGCCGGTGGGGCCCTACCACCACCCCCACGCAGACACGGAGACCCCCGC
CCAACCACCCCGCTACCCCGCCAAGGCCGTCTATCTGCCGCCGCCGCACA
TCGCCCCCCCGGGGCCTCCTCTATCCGGGGCGGTCCCCCCACCCTCGTAT
CCCCCAGTTGCGGTTACCCCCGGTCCCGCTCCCCCGCTACATCAGCCCTC
CCCCGCACACGCCCACCCCCCTCCGCCGCCGCCGGGACCCACGCCTCCCC
CCGCCGCGAGCTTACCCCAACCCGAGGCGCCCGGCGCGGAGGCCGGCGCC
TTAGTTAACGCCAGCAGCGCGGCCCACGTGAACGTGGACACGGCCCGGGC
CGCCGATCTGTTTGTGTCACAGATGATGGGGTCCCGCTAA