Gene ID:HHVTW046
GenBank Locus Tag:
DNA Molecule Name:
1
GenBank ID:
1869866
Gene Name:
UL43
Definition:
unknown
Cellular Location:
[Evidence]
Gene Start:
95433
Gene Stop:
96677
Gene Length:
1245
Molecular Weight*:
42461
pI*:
8.40
Net Charge*:
7.93
EC:
Functional Class:
Gene Ontology:
Pathway: pathway table
Primary Evidence:
DiPaolo JA, Woodworth CD, Coutlee F, Zimonic DB, Bryant J, Kessous A, Relationship of stable integration of herpes simplex virus-2 Bg/II N subfragment Xho2 to malignant transformation of human papillomavirus-immortalized cervical keratinocytes, Int J Cancer 1998 Jun 10;76(6):865-71, Medline: 98289656.
Secondary Evidence:
Carter KL, Ward PL, Roizman B, Characterization of the products of the U(L)43 gene of herpes simplex virus 1: potential implications for regulation of gene expression by antisense transcription, J Virol 1996 Nov;70(11):7663-8, Medline: 97048045.
Ward PL, Barker DE, Roizman B, A novel herpes simplex virus 1 gene, UL43.5, maps antisense to the UL43 gene and encodes a protein hich colocalizes in nuclear structures with capsid proteins, J Virol 1996 May;70(5):2684-90, Medline: 96186692.
MacLean CA, Efstathiou S, Elliott ML, Jamieson FE, McGeoch D, nvestigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins, J Gen Virol 1991 Apr;72 ( Pt 4):897-906, Medline: 91202126.
McGeoch DJ, Dalrymple MA, Davison AJ, Dolan A, Frame MC, McNab D, Perry LJ, Scott JE, Taylor P, The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1, J Gen Virol 1988 Jul;69 ( Pt 7):1531-74, Medline: 88274327.
Comment:
Ward, Barker and Roizman (1996) locate a gene which codes antisense to UL43 and label it UL43.5 and Carter, Ward and Roizman (1996) hypothesize a mechanism of gene regulation based on antisense transcripts. The amino acid sequence predicts the HSV-1 UL43 protein to be a membrane-associated protein, however the HSV-2 protein has a lower net charge, isoelectric point and no predicted transmembrane segments. In HSV-1 UL43 is dispensable for growth in cell culture (MacLean et al. 1991) and Roizman assigns it gamma expression regulation (Roizman, personal communication).
Blast Summary: PSI-Blast Search
Residues 8-414 of the UL43 protein are 64% similar using gapped BLAST to residues 17-433 of UL43 in Human herpesvirus 1 (strain 17). There are no other similarities to other Alphaherpesvirinae or any other organism. Variants include AAA66441.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
No significant hits.
ProDom Summary: Protein Domain Search
Residues 24-414 constitute the membrane protein domain as seen in P89464_VVVVV. Residues 105-362 are 26% similar to a integral membrane protein domain as seen in O86746_STRCO and residues 26-120 are 38% similar to a TAP2 ABC-transporter domain as seen in Q28435_GORGO.
Paralogs: Local Blast Search
None.
Pfam Summary: Pfam Search
None.
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular |
Top PDB Hits:
None.
Gene Protein Sequence:
MCRGDSPGVAGGSGEHCLGGDDGDDGRPRLACVGAIARGFAHLWLQAATL
GFVGSVVLSRGPYADAMSGAFVIGSTGLGFLRAPPAFARPPTRVCAWLRL
VGGGAAVALWSLGEAGAPPGVPGPATQCLALGAAYAALLVLADDVHPLFL
LAPRPLFVGTLGVVVGGLTIGGSARYWWIDPRAAAALTAAVVAGLGTTAA
GDSFSKACPRHRRFCVVSAVESPPPRYAPEDAERPTDHGPLLPSTHHQRS
PRVCGDGAARPENIWVPVVTFAGALALAACAARGSDAAPSGPVLPLWPQV
FVGGHAAAGLTELCQTLAPRDLTDPLLFAYVGFQVVNHGLMFVVPDIAVY
AMLGGAVWISLTQVLGLRRRLHKDPDAGPWAAATLRGLFFSVYALGFAAG
VLVRPRMAASRRSG$
Gene Nucleotide Sequence: Sequence Viewer
ATGTGTCGCGGGGACAGCCCCGGGGTCGCGGGCGGGAGCGGCGAACACTG
CCTCGGAGGGGATGATGGGGACGACGGGCGCCCCCGCCTCGCCTGCGTGG
GTGCCATCGCTCGGGGGTTCGCGCATCTCTGGCTCCAGGCCGCCACGCTG
GGCTTCGTGGGGTCTGTCGTTCTGTCGCGCGGCCCGTATGCGGACGCCAT
GTCGGGGGCGTTCGTGATCGGGAGCACCGGCCTGGGGTTCCTCCGCGCCC
CCCCCGCGTTCGCCCGGCCGCCGACGCGTGTGTGCGCGTGGCTGAGGCTG
GTCGGCGGGGGAGCGGCCGTGGCCCTGTGGAGCCTCGGGGAGGCCGGCGC
GCCTCCGGGGGTTCCGGGCCCGGCGACCCAGTGCCTGGCGCTCGGGGCCG
CCTACGCGGCGCTGCTGGTGCTGGCCGACGACGTCCATCCCCTTTTCCTC
CTCGCCCCGCGGCCCCTGTTTGTCGGCACCCTGGGGGTTGTCGTCGGCGG
GCTGACGATAGGCGGCAGTGCGCGCTACTGGTGGATCGACCCCCGCGCCG
CCGCGGCCCTGACGGCGGCGGTGGTGGCGGGCCTCGGGACAACCGCCGCC
GGGGACAGCTTTTCCAAGGCCTGTCCCCGCCACCGCCGCTTTTGCGTCGT
CTCCGCGGTCGAGTCTCCCCCGCCCCGATACGCCCCGGAGGACGCCGAGC
GGCCAACAGACCACGGACCCCTGTTACCGTCGACGCACCACCAGCGATCT
CCGCGGGTCTGCGGCGACGGGGCCGCACGGCCCGAAAACATCTGGGTTCC
CGTGGTGACCTTTGCGGGCGCGCTCGCGCTGGCCGCCTGCGCCGCGCGAG
GGTCTGACGCGGCTCCGTCAGGCCCGGTCCTGCCGCTGTGGCCCCAGGTG
TTTGTCGGGGGCCACGCGGCGGCGGGCCTGACGGAGCTGTGTCAGACCCT
CGCGCCCCGGGACCTCACGGACCCGCTGCTGTTTGCGTACGTCGGATTCC
AGGTCGTGAACCACGGGCTGATGTTTGTGGTCCCCGACATCGCCGTATAC
GCGATGCTGGGGGGCGCCGTGTGGATCTCGCTGACGCAGGTGCTTGGGCT
CCGGCGCCGCCTTCACAAGGACCCAGACGCCGGGCCCTGGGCGGCCGCGA
CCCTGCGGGGCCTCTTTTTCTCCGTCTACGCATTGGGGTTTGCGGCGGGG
GTGCTGGTGCGGCCGCGGATGGCGGCGAGCCGGCGGTCGGGGTGA