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Record: 1 of 1 MiniMap

LANL Gene ID: PG0031GenBank Locus Tag: PG0035DNA Molecule Name: 1 GenBank ID: 34539911Gene Name: dnaE Definition: DNA polymerase III alpha subunitCellular Location: Cytoplasm [Evidence]Gene Start: 46374Gene Stop: 42691Gene Length: 3684Molecular Weight*: 139199pI*: 5.20Net Charge*: -32.17EC: 2.7.7.7 Functional Class: DNA metabolism; DNA replication, recombination and repair Gene Ontology: Biological process GO:0006260 DNA replication GO:0006355 regulation of transcription, DNA-dependent GO:0007165 signal transduction Cellular component GO:0005737 cytoplasm Molecular function GO:0003676 nucleic acid binding GO:0003677 DNA binding GO:0003887 DNA-directed DNA polymerase activity GO:0003889 alpha DNA polymerase activity GO:0008408 3'-5' exonuclease activityPathway: pathway table Nucleotide Metabolism; Purine metabolism Nucleotide Metabolism; Pyrimidine metabolismComment: The alpha chain is responsible for the polymerase activity of the multichain enzyme. Other subunits -- PG1614, PG1615, PG1245 -- perform 3' to 5' exonucleolytic activity. For other pol III subunit sequences see PG0831, PG1614, PG1615, and PG1245. TIGR ID: PG0035Proteomic Data: Proteomic Data Search

TIGR Annotation		DNA polymerase III, alpha subunit, GroupF (DNA metabolism)
ORF #		PG-PP/PG-PPC1				PG-PP/PG-PPC2
		Spectral Count		Peptide Intensity		Spectral Count		Peptide Intensity
		Pro	qVal	Pro	qVal	Pro	qVal	Pro	qVal
TIGR	PG0035	-1.79	0.00014	-1.26	0.036	0.23	0.28	-1.26	0.036
LANL	PG0031	0.29		0.42		1.17		2.11
Consensus		SP1		IP1		SP2		IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer Blast Summary: PSI-Blast Search Numerous significant hits in gapped BLAST to DNA polymerase III; e.g. residues 4-1201 are 39% similar to DNA polymerase III, alpha chain of Borrelia burgdorferi (AE001159) , residues 4-1190 are 36% similar to the DNA polymerase III of Thermus aquaticus (AF062920), residues 4-1180 are 36% similar to the DNA polymerase III alpha subunit of Aquifex aeolicus (AE000718). This sequence is similar to CT545 and BT2230. Top Blast Hits: Updated monthly Click here to view the entire PsiBlast results. gi|34539911|ref|NP_904390.1| DNA polymerase III, alpha subu... 2488 0.0 gi|154491985|ref|ZP_02031611.1| hypothetical protein PARMER... 1598 0.0 gi|150010065|ref|YP_001304808.1| DNA polymerase III alpha s... 1575 0.0 gi|53712001|ref|YP_097993.1| DNA polymerase III alpha subun... 1534 0.0 gi|60680202|ref|YP_210346.1| putative DNA polymerase III al... 1530 0.0 gi|156859512|gb|EDO52943.1| hypothetical protein BACUNI_037... 1512 0.0 gi|29347640|ref|NP_811143.1| DNA polymerase III alpha subun... 1509 0.0 gi|156109469|gb|EDO11214.1| hypothetical protein BACOVA_031... 1496 0.0 gi|153808691|ref|ZP_01961359.1| hypothetical protein BACCAC... 1488 0.0 gi|150004438|ref|YP_001299182.1| DNA polymerase III alpha s... 1479 0.0InterPro Summary: InterProScan InterPro IPR001610 Repeat PAC motif SM00086 PAC InterPro IPR003141 Domain Polymerase and histidinol phosphatase, N-terminal SM00481 POLIIIAc InterPro IPR004013 Domain PHP, C-terminal PF02811 PHP InterPro IPR004365 Domain Nucleic acid binding, OB-fold, tRNA/helicase-type PF01336 tRNA_anti InterPro IPR004805 Family DNA polymerase III, alpha subunit TIGR00594 polc InterPro IPR011708 Domain Bacterial DNA polymerase III, alpha subunit PF07733 DNA_pol3_alpha noIPR unintegrated unintegrated SSF89550 SSF89550 COGS Summary: COGS Search BeTs to 12 clades of COG0587 COG name: DNA-dependent DNA polymerase III alpha chain Functional Class: L The phylogenetic pattern of COG0587 is -----qvCeBRhujgpolinx Number of proteins in this genome belonging to this COG is 1 Blocks Summary: Blocks Search ***** BP01693 (DNA POLYMERASE PROTEIN III ALP) with a combined E-value of 2.3e-19. BP01693 422-465 ProDom Summary: Protein Domain Search Residues 454-807 are 47% similar to a (DNA POLYMERASE III ALPHA) protein domain (PD004180 which is seen in DP3A_BORBU. Residues 813-1191 are 32% similar to a (DNA POLYMERASE III ALPHA) protein domain (PD004426 which is seen in DP3A_HAEIN. Residues 145-415 are 46% similar to a (DNA POLYMERASE ALPHA III) protein domain (PD003212 which is seen in DP3A_BORBU. Residues 423-453 are 83% similar to a (DNA III POLYMERASE ALPHA) protein domain (PD005473 which is seen in DP3A_BACSU. Residues 4-57 are 55% similar to a (DNA POLYMERASE ALPHA PROTEIN) protein domain (PD001693 which is seen in DP3A_BORBU. Paralogs: Local Blast Search No paralogs found in P. gingivalis. Pfam Summary: Pfam Search Residues 5 to 66 (E-value = 4.7e-22) place PG0031 in the PHP_N family which is described as PHP domain N-terminal region (PF02231) Residues 143 to 283 (E-value = 3.6e-31) place PG0031 in the PHP_C family which is described as PHP domain C-terminal region (PF02811) Structural Feature(s): Feature Type Start Stop coil-coil 66 106 Top PDB Hits: -56% similar to PDB:2HPI Eubacterial and Eukaryotic Replicative DNA Polymerases are not Homologous: X-ray Structure of DNA Polymerase III (E_value = ); -56% similar to PDB:2HPM Eubacterial and Eukaryotic Replicative DNA Polymerases are not Homologous: X-ray Structure of DNA Polymerase III (E_value = ); -60% similar to PDB:2HNH Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III (E_value = 1.1E_167); -60% similar to PDB:2HQA Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III (E_value = 1.1E_167); Gene Protein Sequence: MEPFVHLHVHSQFSLLDGQAAINDLVDKAIADGMPGIALTDHGAMFGIKE FYNYVEKKNSGHNATLKDCRRELDQLNDSKSADMLTDEERNRIVKLQQKI EETKKLLFKPILGCEAYCARRTRFDKDNQIPDPYHPRRSIDASGWHLILL AKNLRGYKNLIKMVSYSWTEGQYYRPRIDKELLQKYHEGIIVSSACLGGE IPQHIMAGEIAKAEEAILWFKELFGDDYYLEIQRHETHNPLGNQDVFPQQ QRVNRVILELGKKLGVKVIATNDVHFCNEEDAEAHDRLICLSTGKDLDDP NRMRYTKQEWMKTTAEMSAIFEDLPETLSNTLEILDKVELYSIDNKALMP DFPIPPEYKDDDDYLRFLTYEGARRKYGEDLSDEIKERIDFELETIKGMG FPGYFLIVQDFIAAARSMGVSVGPGRGSAAGSAVAYCLGITDIDPIKYHL LFERFLNPDRISMPDIDVDFDDDGRAEILRWVTEKYGKERVAHIITYGTM ATKSSIKDVARVQRLPLLESNRLAKLVPDKIPGEKKVNLKKAIEFVPELK QASLSSDKVMRDTLKYAQMLEGNVRNTGVHACGIIIGKTDISDVVPVSTA PDKDTKEELLVTQYEGSVIEQTGLIKMDFLGLKTLSIIKEALVNIKRRHG IDLNIDTIPLDDPLTYKLYSDGRTIGTFQFESGGMQKYLRELQPSAFEDL IAMNALYRPGPMDYIPSFIARKHGREPIDYDLPEMEEYLKETYGVTVYQE QVMLLSRKLAGFTRGQSDELRKAMGKKLIEKMNVLKVKFLEGGNKNGHPE EVLEKIWTDWEKFASYAFNKSHATCYSWVAYQTAYLKANYPAEYMAGALS RNLNNITEITKLMDECKSMKIGVLVPDVNESEMKFSVNANGDIRFGLSAV KGVGSGAVEQIIAEREANGLYKDIFDFVERINLSACNRKTMESLALAGAF DSFALSREEYMAPPLTGREESYIQALMRYGSVVQEEKHSQSNSLFGEEED LMIPRPQPSPTEPWNDLERLNKERELVGLYLSSNPMAQYQVILDHYCNTH AADLTDPDSLVGKNLVLAGIVTKAFQGISRSNNPYSKITLEDLSGTGEIP LFGQAHVNFGNYCKEGLYLLIRASVQPHKWKEGEMELVVTSIELLPQVAD TLIKKMTILLPASKIDNELIEMLSDELTNNSGKTMLFIKVYDHTETFDVE LAQQKSLIKVSPELVNRLKTYEINFALE Gene Nucleotide Sequence: Sequence Viewer ATGGAACCCTTCGTACACCTGCACGTACACTCCCAATTCTCTCTGCTTGA CGGACAGGCCGCTATCAATGACTTGGTGGACAAAGCCATTGCCGACGGTA TGCCGGGTATTGCTCTGACCGATCATGGAGCCATGTTCGGCATCAAGGAG TTTTACAACTATGTGGAGAAGAAGAACAGCGGGCACAATGCGACATTGAA GGACTGTAGGCGCGAACTGGATCAACTGAACGACTCGAAGAGTGCCGACA TGCTGACCGATGAAGAGCGCAACCGCATTGTCAAGTTGCAGCAGAAGATA GAGGAGACCAAAAAACTCCTCTTCAAACCTATCCTTGGGTGTGAGGCATA TTGTGCTCGACGTACGCGATTCGACAAGGATAACCAGATACCGGATCCGT ATCATCCCAGACGCTCCATCGATGCCAGCGGTTGGCACTTGATCCTGCTG GCAAAAAACTTGCGAGGGTACAAGAATCTGATCAAAATGGTCAGCTATTC ATGGACAGAAGGACAGTACTACCGTCCGCGTATCGACAAGGAGCTTTTGC AGAAGTATCATGAAGGTATCATTGTCTCTTCGGCCTGCCTGGGAGGAGAG ATACCACAGCATATCATGGCAGGAGAGATTGCCAAGGCTGAGGAGGCCAT CCTCTGGTTCAAAGAGCTTTTTGGGGACGATTACTATTTGGAGATTCAGC GACACGAGACGCATAATCCACTCGGCAATCAGGATGTTTTCCCCCAGCAA CAGCGCGTAAATCGCGTCATACTGGAGCTTGGCAAGAAGTTGGGCGTCAA AGTTATTGCGACGAACGATGTCCATTTCTGCAATGAAGAAGATGCTGAGG CGCACGACCGTCTGATCTGTCTGAGCACAGGGAAGGATCTGGACGATCCG AACAGGATGCGCTATACCAAGCAGGAATGGATGAAGACTACGGCAGAGAT GAGCGCCATATTCGAGGATCTACCTGAGACTCTTTCCAACACATTAGAAA TACTTGACAAGGTAGAGTTATACAGCATCGACAACAAAGCCCTGATGCCG GACTTTCCTATCCCCCCCGAATATAAAGACGATGATGACTACCTGCGTTT CCTTACCTACGAAGGGGCCAGGCGCAAGTATGGAGAGGATCTTTCCGATG AGATAAAAGAGCGTATCGACTTTGAACTGGAAACCATCAAAGGAATGGGG TTCCCCGGCTACTTCCTTATTGTACAGGATTTCATTGCAGCCGCACGAAG TATGGGCGTCTCTGTCGGACCCGGCCGAGGATCGGCAGCCGGTTCGGCGG TAGCTTACTGTTTGGGGATCACGGATATCGATCCGATCAAGTACCATTTG CTTTTCGAGCGTTTTCTCAATCCGGATCGTATCTCCATGCCCGATATAGA CGTCGACTTCGATGATGACGGGCGTGCCGAAATACTCCGCTGGGTGACGG AAAAATACGGCAAGGAGAGAGTGGCCCACATCATCACCTACGGTACGATG GCCACAAAGAGTTCTATTAAGGATGTGGCTCGCGTACAGAGGCTGCCCTT ACTCGAAAGCAATCGTTTAGCCAAGCTCGTACCAGACAAAATTCCCGGAG AGAAGAAAGTCAATCTGAAGAAAGCAATCGAATTTGTTCCAGAGCTGAAG CAAGCATCGCTAAGCTCCGATAAGGTAATGCGCGATACCCTGAAATACGC CCAAATGCTGGAGGGGAATGTGCGCAATACAGGTGTGCATGCCTGCGGTA TCATCATTGGGAAAACGGATATCAGCGATGTCGTTCCGGTCAGTACGGCT CCCGACAAAGACACGAAGGAGGAGCTGCTGGTCACACAATACGAAGGCTC TGTCATCGAACAGACGGGACTGATCAAAATGGACTTCCTCGGGCTGAAAA CGCTCTCGATCATCAAAGAAGCTTTGGTCAACATAAAGCGGCGCCATGGC ATTGACTTGAATATAGACACGATCCCTCTCGACGATCCTCTTACATATAA GCTATACAGCGATGGACGTACCATAGGTACTTTCCAATTCGAATCCGGTG GGATGCAAAAGTATCTTAGGGAGCTACAGCCCTCTGCTTTCGAGGACTTG ATAGCGATGAATGCTCTCTATCGTCCCGGTCCGATGGACTATATCCCTTC TTTTATCGCACGCAAGCATGGGCGTGAGCCTATCGATTACGACCTGCCTG AGATGGAAGAATATCTCAAAGAAACTTATGGTGTGACGGTGTATCAGGAG CAAGTCATGCTTCTGAGTCGCAAGCTTGCCGGTTTTACCCGAGGCCAGAG CGATGAGCTTCGTAAGGCTATGGGCAAAAAACTCATCGAAAAGATGAATG TTCTGAAGGTGAAGTTTCTCGAGGGGGGGAATAAGAATGGACATCCGGAA GAAGTTCTGGAAAAGATCTGGACAGACTGGGAGAAGTTCGCCAGCTATGC TTTCAACAAGAGTCATGCCACCTGCTACAGTTGGGTAGCCTACCAAACGG CTTACCTGAAAGCAAACTATCCTGCTGAATATATGGCAGGAGCATTGAGC CGAAACCTGAATAACATCACGGAAATAACCAAGCTCATGGATGAGTGCAA GTCCATGAAGATCGGCGTATTGGTACCGGATGTGAACGAGTCCGAAATGA AATTCTCAGTAAATGCCAATGGGGATATTCGGTTCGGTCTCAGCGCTGTA AAGGGTGTAGGATCGGGAGCCGTGGAGCAGATCATTGCCGAACGCGAAGC CAACGGGCTATATAAGGACATCTTCGACTTTGTGGAGCGGATCAATCTCT CTGCCTGCAATCGCAAGACGATGGAGAGTCTGGCCTTGGCCGGTGCTTTC GACAGCTTCGCTCTATCCCGGGAGGAATATATGGCTCCTCCTCTGACAGG AAGAGAGGAGTCATACATACAAGCACTTATGCGCTATGGGTCCGTAGTAC AAGAGGAGAAGCACAGCCAGAGCAATTCCCTCTTCGGAGAGGAAGAAGAT CTGATGATTCCTCGTCCGCAGCCATCTCCCACCGAGCCATGGAACGACTT GGAAAGGCTCAATAAAGAACGTGAACTCGTAGGGCTCTACCTTAGTTCCA ATCCAATGGCTCAGTACCAAGTCATTCTCGATCACTATTGCAATACCCAT GCAGCCGATTTGACCGATCCGGATTCCTTGGTCGGGAAAAATTTGGTTTT GGCCGGTATTGTTACCAAGGCTTTTCAGGGAATCTCTCGCTCGAATAATC CGTACTCAAAGATTACGTTAGAGGATCTGTCCGGCACGGGCGAAATACCT CTTTTCGGACAGGCTCATGTAAACTTCGGCAATTATTGCAAGGAGGGGCT GTATCTCCTCATTCGCGCATCCGTTCAGCCTCATAAGTGGAAAGAGGGTG AAATGGAATTGGTCGTGACCTCAATCGAACTGCTACCTCAAGTGGCCGAT ACATTGATCAAAAAGATGACGATCCTACTGCCGGCATCGAAGATCGACAA TGAGCTGATCGAAATGCTGTCTGACGAATTGACAAACAATTCGGGGAAAA CGATGTTATTTATCAAAGTGTATGACCATACGGAGACTTTCGATGTCGAA CTGGCACAGCAGAAATCGCTGATCAAAGTGTCCCCCGAACTGGTGAATAG ATTGAAGACTTATGAGATTAACTTTGCACTTGAA Los Alamos National Laboratory • Est 1943 Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy

`Feature Type`	`Start`	`Stop`
`coil-coil`	`66`	`106`