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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0033 IGR0035 IGR0031 IGR0034 IGR0036 IGR0032 dnaE, - PG0031 PG0037 rl19, - PG0032 ISPg5, - PG0033 ISPg5, - PG0034 glyA, - PG0035 PG0036 dnaE, - PG0031 PG0037 rl19, - PG0032 ISPg5, - PG0033 ISPg5, - PG0034 glyA, - PG0035 PG0036 Type: IS element, Name: ISPg5 - 8 dnaE, - PG0031 PG0037 rl19, - PG0032 ISPg5, - PG0033 ISPg5, - PG0034 glyA, - PG0035 PG0036


LANL Gene ID: PG0034

GenBank Locus Tag: PG0041

DNA Molecule Name:
1  

Genomic Island ID:
20

GenBank ID:
34539915

 Gene Name:
ISPg5  

 Definition:
ISPg5 transposase

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
48517

Gene Stop:
49452

Gene Length:
936

Molecular Weight*:
36274

pI*:
10.20

Net Charge*:
15.52

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0015074    DNA integration

Molecular function
  GO:0003677    DNA binding


 Pathway: pathway table

 Primary Evidence:
Califano,J.V., Kitten,T., Lewis,J.P., Macrina,F.L., Fleischmann,R.D., Fraser,C.M.,Duncan,M.J. and Dewhirst,F.E. Characterization of Porphyromonas gingivalis insertion
sequence-like element.
ISPg5 Infect. Immun. 68 (9), 5247-5253 (2000)

 Secondary Evidence:
Xu,J., Bjursell,M.K., Himrod,J., Deng,S., Carmichael,L.K.,
Chiang,H.C., Hooper,L.V. and Gordon,J.I.
A Genomic View of the Human-Bacteroides thetaiotaomicron Symbiosis
Science 299 (5615), 2074-2076 (2003)
PUBMED: 12663928

 Comment:
One of eleven ISPg5 elements in the genome (see Paralogs). Califano et al. report considerable strain diversity of these (Infect. Immun. 68 (9), 5247-5253 (2000)).

Member of the IS3 family of insertion elements. See PG0033 for associated ORF.

TIGR ID: PG0041

 Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0034 is equivalent to the previously reported gb|AAF69128.1| in GenBANK.

Numerous significant hits in gapped BLAST to transposase protein; e.g. residues 91-213 are 56% similar to (U67062) transposase homolog of Bacteroides thetaiotaomicron, residues 64-287 are 32% similar to (AF153317) InsB of Shigella dysenteriae, residues 64-268 are 33% similar to (AF081284) Hp1 of Escherichia coli.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539915|ref|NP_904394.1|  ISPg5 transposase Orf2 [Porphy...   630   e-179
 gi|34540277|ref|NP_904756.1|  ISPg5, transposase Orf2 [Porph...   627   e-178
 gi|34540399|ref|NP_904878.1|  ISPg5, transposase Orf2 [Porph...   624   e-177
 gi|34541341|ref|NP_905820.1|  ISPg5, transposase Orf2 [Porph...   622   e-177
 gi|34540248|ref|NP_904727.1|  ISPg5, transposase Orf2 [Porph...   621   e-176
 gi|118200270|dbj|BAF37077.1|  transposase Orf2 [Porphyromona...   620   e-176
 gi|34541287|ref|NP_905766.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34540696|ref|NP_905175.1|  ISPg5, transposase Orf2 [Porph...   617   e-175
 gi|34541641|ref|NP_906120.1|  ISPg5, transposase Orf2 [Porph...   617   e-175
 gi|34539888|ref|NP_904367.1|  ISPg5 transposase Orf2 [Porphy...   612   e-173


InterPro Summary:  InterProScan

InterPro
IPR001584
Domain
Integrase, catalytic core
PF00665 [110-273]T 8.1e-31 PF00665 rve rve
PS50994 [110-276]T 18.357 PS50994 INTEGRASE INTEGRASE
noIPR
unintegrated
unintegrated
G3DSA:3.30.420.10 [113-281]T 2.9e-06 G3DSA:3.30.420.10 no description no description


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01836 (Transposase) with a combined E-value of 4.3e-23.
    PF01836B    103-154
    PF01836C    181-190
    PF01836D    215-222
    PF01836E    253-272


 ProDom Summary:  Protein Domain Search
No significant hit to the ProDom database.

Paralogs:  Local Blast Search
PG0034 is paralogously related to PG1861, PG1491, PG0391, PG0418, PG1436, PG1796, PG0007, PG0533, PG1247 and PG0841, all ORFs associated with ISPg5 transposases.

Pfam Summary:  Pfam Search
Residues 110 to 273 (E-value = 8.3e-31) place PG0034 in the rve family which is described as Integrase core domain (PF00665)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
LLGFSRQAFYKRHLNDLARHEEDVLCSSIIQYCWHLRQAEHLPQAGFREL
MVLCQQYFGPKFTLGRDRFCALLRRHGMMLRKRSVRPRTTNSRHRLYKYE
DLLNTEPKFVPQRPGELLVADITYVAYQDGFAYLSLLTDAYSRCIVGYCL
HPTLEVEGCLNALHQAFAFYDQHQIDTSNMIHHSDRGIQYAGKSYTDLLH
GRGWRISMTQTGDPLHNALAERMNNTLKNSWHISSSKQSFDQALLSVEQA
VRMYNEARPHQALGAKTPMQVIAPESKNPLLTRREHGPEIAPELYRRMNV
RQRANFARVNRN

Gene Nucleotide Sequence:  Sequence Viewer
CTGCTTGGCTTTAGCCGTCAGGCCTTTTATAAACGTCATCTCAACGATTT
AGCTAGGCACGAGGAGGATGTCCTATGCAGTAGTATCATCCAATATTGTT
GGCACTTAAGACAAGCAGAGCACCTACCTCAAGCCGGCTTCCGAGAGCTC
ATGGTGCTTTGTCAGCAATACTTCGGCCCCAAGTTCACGCTTGGGCGCGA
TCGCTTTTGCGCCCTACTCAGACGCCATGGTATGATGCTGCGCAAACGGT
CTGTGCGCCCTCGGACAACCAACTCTCGGCATAGGCTCTACAAGTATGAA
GACCTGCTCAATACAGAGCCTAAGTTTGTGCCTCAAAGACCTGGGGAACT
CCTTGTAGCAGACATCACCTACGTGGCTTACCAAGATGGGTTTGCCTATC
TCTCCCTACTGACCGATGCCTATAGTCGCTGTATCGTAGGGTATTGCTTA
CATCCTACCCTAGAGGTTGAAGGCTGTTTGAATGCTCTACATCAAGCCTT
TGCTTTCTACGATCAGCATCAAATTGATACGAGCAATATGATTCATCATA
GCGACCGAGGCATTCAGTATGCCGGTAAGAGCTACACCGATCTGTTGCAT
GGGCGAGGCTGGCGCATCAGCATGACTCAGACGGGAGATCCTCTGCATAA
TGCATTGGCTGAGCGGATGAACAATACGCTCAAGAACTCGTGGCACATCT
CCTCTTCGAAGCAGTCTTTTGATCAAGCACTCCTTTCTGTAGAACAAGCC
GTGCGCATGTACAACGAGGCGCGTCCACATCAAGCTCTAGGGGCGAAAAC
GCCTATGCAAGTCATTGCGCCAGAGTCTAAAAATCCGTTACTAACGAGGA
GGGAGCATGGGCCAGAGATTGCCCCCGAGCTATACCGAAGGATGAATGTC
AGACAAAGAGCTAACTTTGCTCGTGTCAACCGAAAT

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