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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0038 IGR0033 IGR0035 IGR0037 IGR0034 IGR0036 IGR0032 cdsA, - PG0040 ftsH, - PG0041 PG0037 ISPg5, - PG0033 ISPg5, - PG0034 glyA, - PG0035 htpG, - PG0038 PG0036 cdsA, - PG0040 ftsH, - PG0041 PG0037 ISPg5, - PG0033 ISPg5, - PG0034 glyA, - PG0035 htpG, - PG0038 PG0036 Type: IS element, Name: ISPg5 - 8 ftsH, - PG0041 cdsA, - PG0040 PG0037 ISPg5, - PG0033 ISPg5, - PG0034 glyA, - PG0035 htpG, - PG0038 PG0036


LANL Gene ID: PG0036

GenBank Locus Tag: PG0043

DNA Molecule Name:
1  

Genomic Island ID:
20

GenBank ID:
34539917

 Gene Name:


 Definition:
beta-N-acetylglucosaminidase

 Cellular Location:
Cytoplasm, Outer membrane, Periplasm, Extracellular [Evidence]

 Gene Start:
51206

Gene Stop:
53536

Gene Length:
2331

Molecular Weight*:
87656

pI*:
5.60

Net Charge*:
-14.67

EC:
3.2.1.96  

 Functional Class:
Energy metabolism; Biosynthesis and degradation of polysaccharides  

 Gene Ontology:
Biological process
  GO:0005975    carbohydrate metabolic process

Molecular function
  GO:0003824    catalytic activity
  GO:0004563    beta-N-acetylhexosaminidase activity
  GO:0043169    cation binding


 Pathway: pathway table
Glycoprotein degradation

 Comment:
TIGR ID: PG0043

 Proteomic Data: Proteomic Data Search
TIGR Annotationbeta-hexosaminidase, GroupC (cell envelope)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG00430.230.10.110.20.874.1e-060.110.2
LANLPG00361.171.081.831.17
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0036 is equivalent to the previously reported sp|P49008|HEXA_PORGI in GenBANK, a beta-N-acetylhexosaminidase.

Numerous significant hits in gapped BLAST to beta-N-acetylglucosaminidase protein; e.g. residues 37-531 are 39% similar to (AF072374) beta-N-acetylglucosaminidase of Pseudoalteromonas, residues 36-540 are 41% similar to (AL138851) putative sugar hydrolase of Streptomyces coelicolor, residues 27-499 are 35% similar to (U41417) beta-N-acetylhexosaminidase of Vibrio furnissii].

This sequence is similar to BT1051.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539917|ref|NP_904396.1|  beta-hexosaminidase [Porphyrom...  1635   0.0  
 gi|488526|emb|CAA55582.1|  beta-N-acetylhexosaminidase [Porp...  1624   0.0  
 gi|154492189|ref|ZP_02031815.1|  hypothetical protein PARMER...   872   0.0  
 gi|150007911|ref|YP_001302654.1|  glycoside hydrolase family...   858   0.0  
 gi|28274157|gb|AAO33832.1|  HexA [Tannerella forsythensis]        857   0.0  
 gi|53715534|ref|YP_101526.1|  beta-hexosaminidase precursor ...   748   0.0  
 gi|60683487|ref|YP_213631.1|  putative beta-hexosaminidase p...   748   0.0  
 gi|156859206|gb|EDO52637.1|  hypothetical protein BACUNI_034...   514   e-143
 gi|89891626|ref|ZP_01203130.1|  beta-acetylhexosaminidase/pr...   507   e-141
 gi|146298437|ref|YP_001193028.1|  Beta-N-acetylhexosaminidas...   503   e-140


InterPro Summary:  InterProScan

InterPro
IPR001540
Family
Glycoside hydrolase, family 20
PR00738 [122-142]T 2.5E-49 PR00738 GLHYDRLASE20[164-181]T 2.5E-49 PR00738 GLHYDRLASE20[193-214]T 2.5E-49 PR00738 GLHYDRLASE20[254-271]T 2.5E-49 PR00738 GLHYDRLASE20[302-320]T 2.5E-49 PR00738 GLHYDRLASE20[324-337]T 2.5E-49 PR00738 GLHYDRLASE20[469-485]T 2.5E-49 PR00738 GLHYDRLASE20[487-504]T 2.5E-49 PR00738 GLHYDRLASE20 GLHYDRLASE20
PF00728 [170-506]T 0.0 PF00728 Glyco_hydro_20 Glyco_hydro_20
InterPro
IPR011658
Domain
PA14
PF07691 [626-766]T 1.99999636034536E-28 PF07691 PA14 PA14
InterPro
IPR013145
Domain
PA14-related
SM00758 [626-765]T 5.49999338756182E-24 SM00758 PA14 PA14
InterPro
IPR013781
Domain
Glycoside hydrolase, catalytic core
G3DSA:3.20.20.80 [168-534]T 0.0 G3DSA:3.20.20.80 Glyco_hydro_cat Glyco_hydro_cat
noIPR
unintegrated
unintegrated
G3DSA:3.30.379.10 [33-166]T 1.2000011745813998E-31 G3DSA:3.30.379.10 G3DSA:3.30.379.10 G3DSA:3.30.379.10
PTHR22600 [112-724]T 0.0 PTHR22600 PTHR22600 PTHR22600
PS51257 [1-19]T 0.0 PS51257 PROKAR_LIPOPROTEIN PROKAR_LIPOPROTEIN
SSF51445 [170-520]T 2.5000000000000004E-125 SSF51445 SSF51445 SSF51445
SSF55545 [24-169]T 2.4E-27 SSF55545 SSF55545 SSF55545
SSF56988 [683-737]T 3.5999999999999997E-4 SSF56988 SSF56988 SSF56988


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PR00738 (Glycosyl hydrolase family 20 signature) with a combined E-value of 4.4e-81.
    PR00738A    122-142
    PR00738B    164-181
    PR00738C    193-214
    PR00738D    254-271
    PR00738E    302-320
    PR00738F    324-337
    PR00738G    469-485
    PR00738H    487-504


 ProDom Summary:  Protein Domain Search
Residues 1-135 are identical to a (BETA-HEXOSAMINIDASE PRECURSOR EC 3.2.1.52) protein domain (PD138810 which is seen in HEXA_PORGI.

Residues 307-503 are 27% similar to a (HYDROLASE GLYCOSIDASE CHITOBIASE) protein domain (PD018185 which is seen in HEX_VIBVU.

Residues 532-777 are 99% similar to a (BETA-HEXOSAMINIDASE PRECURSOR EC 3.2.1.52) protein domain (PD138814 which is seen in HEXA_PORGI.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
Residues 170 to 507 (E-value = 7.5e-63) place PG0036 in the Glyco_hydro_20 family which is described as Glycosyl hydrolase family 20, catalytic domain (PF00728)

 Structural Feature(s):
Feature Type  Start  Stop
may be a lipoprotein  
  
gram negative signal  
1  
23

Top PDB Hits:
-47% similar to PDB:1HP4 CRYSTAL STRUCTURE OF STREPTOMYCES PLICATUS BETA-N-ACETYLHEXOSAMINIDASE (E_value = 7.3E_64);
-47% similar to PDB:1HP5 STREPTOMYCES PLICATUS BETA-N-ACETYLHEXOSAMINIDASE COMPLEXED WITH INTERMEDIATE ANALOUGE NAG-THIAZOLINE (E_value = 7.3E_64);
-47% similar to PDB:1JAK Streptomyces plicatus beta-N-acetylhexosaminidase in Complex with (2R,3R,4S,5R)-2-acetamido-3,4-dihydroxy-5-hydroxymethyl-piperidinium chloride (IFG) (E_value = 7.3E_64);
-47% similar to PDB:1M01 Wildtype Streptomyces plicatus beta-hexosaminidase in complex with product (GlcNAc) (E_value = 7.3E_64);
-47% similar to PDB:1M04 Mutant Streptomyces plicatus beta-hexosaminidase (D313N) in complex with product (GlcNAc) (E_value = 2.8E_63);

Gene Protein Sequence:
MKRLTFGACICCLLSLMACSQKAKQVQIPEYDKGINIIPLPMQLTESDDS
FEVDDKTTICVSAEELKPIAKLLADKLRASADLSLQIEIGEEPSGNAIYI
GVDTALPLKEEGYMLRSDKRGVSIIGKSAHGAFYGMQTLLQLLPAEVESS
NEVLLPMTVPGVEIKDEPAFGYRGFMLDVCRHFLSVEDIKKHIDIMAMFK
INRFHWHLTEDQAWRIEIKKYPRLTEVGSTRTEGDGTQYSGFYTQEQVRD
IVQYASDRFITVIPEIEMPGHAMAALAAYPQLACFPREFKPRIIWGVEQD
VYCAGKDSVFRFISDVIDEVAPLFPGTYFHIGGDECPKDRWKACSLCQKR
MRDNGLKDEHELQSYFIKQAEKVLQKHGKRLIGWDEILEGGLAPSATVMS
WRGEDGGIAAANMNHDVIMTPGSGGLYLDHYQGDPTVEPVAIGGYAPLEQ
VYAYNPLPKELPADKHRYVLGAQANLWAEYLYTSERYDYQAYPRLLAVAE
LTWTPLAKKDFADFCRRLDNACVRLDMHGINYHIPLPEQPGGSSDFIAFT
DKAKLTFTTSRPMKMVYTLDETEPTLTSTPYTVPLEFAQTGLLKIRTVTA
GGKMSPVRRIRVEKQPFNMSMEVPAPKPGLTIRTAYGDLYDVPDLQQVAS
WEVGTVSSLEEIMHGKEKITSPEVLERRVVEATGYVLIPEDGVYEFSTEN
NEFWIDNVKLIDNVGEVKKFSRRNSSRALQKGYHPIKTIWVGAIQGGWPT
YWNYSRVMIRLKGEEKFKPISSDMLFQ

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAACGACTGACTTTCGGAGCATGCATTTGCTGCCTCCTGTCTCTTAT
GGCCTGCTCACAGAAAGCAAAGCAGGTGCAAATCCCCGAATACGACAAGG
GTATAAACATCATTCCCTTGCCGATGCAGCTGACCGAATCGGACGACAGC
TTTGAGGTCGATGATAAGACCACTATCTGCGTATCTGCCGAAGAGCTAAA
GCCTATCGCTAAACTTCTTGCCGACAAGCTAAGAGCATCAGCCGACCTCT
CTCTCCAGATAGAGATAGGCGAGGAGCCTTCGGGGAATGCTATTTACATC
GGTGTCGATACGGCTCTTCCTCTTAAAGAAGAGGGTTATATGCTCCGATC
CGATAAGCGTGGTGTCAGTATCATCGGCAAATCTGCCCATGGTGCTTTCT
ACGGTATGCAGACTTTGCTCCAGCTCCTTCCTGCCGAAGTGGAATCTTCG
AATGAGGTACTGCTCCCCATGACGGTGCCCGGCGTCGAGATCAAGGACGA
ACCGGCATTCGGCTATCGTGGCTTTATGCTGGATGTATGCCGTCATTTCC
TTTCGGTGGAGGACATCAAGAAGCATATCGACATCATGGCCATGTTCAAG
ATCAATCGTTTCCATTGGCACCTGACAGAGGATCAGGCATGGCGTATCGA
AATCAAGAAATACCCACGACTGACCGAAGTGGGGTCTACAAGGACGGAAG
GGGACGGTACGCAGTACTCCGGTTTCTACACGCAGGAGCAAGTACGGGAT
ATTGTACAATACGCATCGGATCGTTTCATTACGGTGATTCCCGAGATCGA
AATGCCCGGACATGCCATGGCTGCCCTCGCTGCTTATCCGCAGTTGGCTT
GCTTCCCACGCGAATTCAAGCCACGGATTATCTGGGGAGTGGAGCAGGAT
GTTTATTGTGCCGGTAAGGACAGCGTCTTCCGTTTTATCTCTGATGTTAT
CGACGAGGTAGCACCCCTTTTCCCCGGCACATACTTCCATATCGGAGGGG
ACGAATGCCCTAAAGATCGATGGAAGGCTTGTTCGCTTTGTCAGAAGCGT
ATGCGTGACAATGGGTTGAAAGACGAACACGAGCTGCAGAGTTATTTCAT
CAAACAAGCTGAAAAGGTCTTACAAAAGCACGGCAAGAGACTGATCGGTT
GGGATGAAATCCTCGAAGGCGGGCTTGCACCTTCTGCCACCGTTATGAGC
TGGCGTGGAGAGGATGGTGGCATCGCAGCGGCTAATATGAATCACGATGT
GATCATGACTCCGGGTAGCGGAGGTCTCTACTTGGATCATTATCAGGGAG
ATCCGACCGTCGAGCCTGTTGCCATCGGAGGTTATGCTCCATTGGAGCAA
GTGTATGCTTACAATCCTTTGCCGAAAGAATTGCCGGCCGATAAGCATCG
CTACGTGCTCGGAGCACAGGCCAATCTGTGGGCAGAATACCTCTATACTT
CCGAACGATACGACTATCAGGCCTATCCAAGGCTACTGGCTGTGGCAGAG
CTTACCTGGACACCGTTGGCCAAGAAAGATTTTGCCGATTTCTGTCGCCG
TTTGGATAATGCCTGCGTTCGTCTGGACATGCATGGTATCAATTACCACA
TTCCGCTGCCCGAACAACCGGGTGGCTCTTCCGACTTTATAGCCTTTACG
GACAAGGCTAAGCTGACCTTCACGACATCGCGTCCGATGAAAATGGTCTA
TACGCTGGACGAAACCGAACCGACCCTCACATCGACTCCTTACACGGTCC
CTCTTGAATTTGCACAAACGGGCCTTCTGAAGATTCGTACCGTCACGGCC
GGTGGGAAGATGAGTCCCGTACGCCGCATTCGTGTGGAGAAACAACCCTT
CAATATGTCAATGGAAGTACCGGCACCGAAACCCGGACTGACCATTCGTA
CGGCTTACGGTGACTTATATGATGTGCCTGATCTGCAGCAGGTAGCCTCA
TGGGAAGTAGGGACCGTTAGCTCTTTGGAGGAAATCATGCACGGGAAAGA
GAAGATAACTTCTCCTGAAGTACTGGAGCGCAGAGTTGTAGAGGCTACCG
GTTATGTGCTTATTCCGGAGGATGGGGTATATGAGTTCTCTACGGAAAAC
AACGAGTTTTGGATTGATAATGTGAAGCTGATCGACAATGTGGGCGAAGT
AAAGAAATTCTCCCGTCGCAATAGCAGTCGTGCCCTTCAGAAAGGCTACC
ATCCGATCAAGACGATATGGGTCGGAGCCATACAAGGTGGCTGGCCTACT
TATTGGAACTACAGCAGGGTAATGATACGGCTCAAGGGAGAAGAAAAGTT
CAAGCCGATCTCGTCCGATATGCTCTTTCAA

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