Basic Search | Intermediate Search | Advanced Search | Gene Image Map |  Sequence Search |  Protein Motif Search |  Repeat Search |  DNA/RNA Motif Search |  Home

Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0038 IGR0037 IGR0039 IGR0042 IGR0041 IGR0040 IGR0036 cdsA, - PG0040 ychF, - PG0042 ftsH, - PG0041 PG0037 ISPg1, - PG0044 ISPg4,tnp, - PG0043 htpG, - PG0038 PG0036 cdsA, - PG0040 ychF, - PG0042 ftsH, - PG0041 PG0037 ISPg1, - PG0044 ISPg4,tnp, - PG0043 htpG, - PG0038 PG0036 Type: IS element, Name: ISPg1 fragment - 3 Type: IS element, Name: ISPg4 - 7 cdsA, - PG0040 ychF, - PG0042 ftsH, - PG0041 ISPg1, - PG0044 ISPg4,tnp, - PG0043 htpG, - PG0038 PG0036 PG0037


LANL Gene ID: PG0041

GenBank Locus Tag: PG0047

DNA Molecule Name:
1  

Genomic Island ID:
20

GenBank ID:
34539920

 Gene Name:
ftsH  

 Definition:
ATPase/cell division protein/ATP-dependent zinc metallopeptidase

 Cellular Location:
Cytoplasm, Inner membrane, Extracellular [Evidence]

 Gene Start:
59340

Gene Stop:
57322

Gene Length:
2019

Molecular Weight*:
74780

pI*:
5.80

Net Charge*:
-8.41

EC:
3.4.24.-  

 Functional Class:
Cellular processes; Cell division  

 Gene Ontology:
Biological process
  GO:0006508    proteolysis
  GO:0030163    protein catabolic process

Cellular component
  GO:0016020    membrane
  GO:0016021    integral to membrane

Molecular function
  GO:0000166    nucleotide binding
  GO:0004222    metalloendopeptidase activity
  GO:0005524    ATP binding
  GO:0008270    zinc ion binding
  GO:0017111    nucleoside-triphosphatase activity


 Pathway: pathway table

 Secondary Evidence:
Akiyama, Y., T. Yoshihisa, K. Ito 1995. FtsH, a membrane-bound ATPase, forms a complex in the cytoplasmic membrane of Escherichia coli. J.Biol.Chem. 270:23485-23490. PubMed: 7559511.

Akiyama, Y., A. Kihara, H. Tokuda, K. Ito 1996. FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins. J.Biol.Chem. 271:31196-31201. PubMed: 8940120.

 Comment:
The FtsH homolog of B.subtilis apparently functions as an ATP-dependent zinc metalloprotease involved in the degradation of sigma-32. It is an integral membrane protein. FtsH is a member of the AAA family of ATPases and belongs to the M41 peptidase family (zinc metalloproteases).

This sequence contains the MAxxxST motif associated with antigenic mimicry to a heart muscle-specific alpha myosin heavy chain sequence, and implicated in autoimmune myocarditis. See Bachmaier et al., Science, vol 283: Chlamydia Infections and Heart Disease Linked Through Antigenic Mimicry.
NEW VALUE: The FtsH homolog of B.subtilis apparently functions as an ATP-dependent zinc metalloprotease involved in the degradation of sigma-32. It is an integral membrane protein. FtsH is a member of the AAA family of ATPases and belongs to the M41 peptidase family (zinc metalloproteases).


TIGR ID: PG0047

 Proteomic Data: Proteomic Data Search
TIGR Annotationcell division protein FtsH, putative, GroupD (cellular processes)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG00472.031.9e-072.152.6e-062.9502.152.6e-06
LANLPG00414.084.437.746.49
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
In gapped BLAST there are many significant hits to a variety of proteins: a putative AAA-type ATPase from Arabidopsis thaliana (3461848), an ATPase family gene from Homo sapiens (5802970), a FtsH2 homolog from Cyanidioschyzon merolae (6539504), FtsH from Neisseria meningitidis (7226031), B. subtilis (585159), H. influenzae (2492507).

This protein is similar to CT841, a predicted FtsH-related ATPase, and to BT4007.


 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539920|ref|NP_904399.1|  cell division protein FtsH, pu...  1338   0.0  
 gi|150008468|ref|YP_001303211.1|  AAA-metalloprotease FtsH, ...   862   0.0  
 gi|154494841|ref|ZP_02033846.1|  hypothetical protein PARMER...   856   0.0  
 gi|53712067|ref|YP_098059.1|  AAA-metalloprotease FtsH with ...   810   0.0  
 gi|150004472|ref|YP_001299216.1|  AAA-metalloprotease FtsH w...   805   0.0  
 gi|156107224|gb|EDO08969.1|  hypothetical protein BACOVA_048...   798   0.0  
 gi|156859430|gb|EDO52861.1|  hypothetical protein BACUNI_036...   796   0.0  
 gi|153807421|ref|ZP_01960089.1|  hypothetical protein BACCAC...   791   0.0  
 gi|29349415|ref|NP_812918.1|  AAA-metalloprotease FtsH, with...   789   0.0  
 gi|83857895|ref|ZP_00951422.1|  putative transmembrane AAA-m...   704   0.0


InterPro Summary:  InterProScan

InterPro
IPR000642
Domain
Peptidase M41
PF01434 [423-626]T 7.699984805337611E-91 PF01434 Peptidase_M41 Peptidase_M41
InterPro
IPR003593
Domain
AAA+ ATPase, core
SM00382 [225-365]T 1.8999999999998697E-24 SM00382 AAA AAA
InterPro
IPR003959
Domain
AAA ATPase, core
PF00004 [228-416]T 3.60002015030994E-95 PF00004 AAA AAA
InterPro
IPR003960
Domain
AAA ATPase, subdomain
PS00674 [333-351]T 8.0E-5 PS00674 AAA AAA
InterPro
IPR005936
Domain
Peptidase M41, FtsH
TIGR01241 [134-628]T 0.0 TIGR01241 FtsH_fam FtsH_fam
InterPro
IPR011546
Domain
Peptidase M41, FtsH extracellular
PF06480 [43-202]T 3.3000052680717897E-29 PF06480 FtsH_ext FtsH_ext
noIPR
unintegrated
unintegrated
G3DSA:1.10.8.60 [364-432]T 2.90000354677958E-20 G3DSA:1.10.8.60 G3DSA:1.10.8.60 G3DSA:1.10.8.60
G3DSA:3.40.50.300 [178-361]T 2.69998997787803E-60 G3DSA:3.40.50.300 G3DSA:3.40.50.300 G3DSA:3.40.50.300
PTHR23076 [14-617]T 0.0 PTHR23076 PTHR23076 PTHR23076
PTHR23076:SF11 [14-617]T 0.0 PTHR23076:SF11 PTHR23076:SF11 PTHR23076:SF11
SSF52540 [172-562]T 3.4999999999999994E-81 SSF52540 SSF52540 SSF52540


COGS Summary:  COGS Search
BeTs to 13 clades of COG0465
COG name: ATP-dependent Zn proteases
Functional Class: O
The phylogenetic pattern of COG0465 is ----YqvCeBrHUJgpoLinx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** IPB001939 (AAA-protein (ATPases associated with various cellular activities)) with a combined E-value of 8.3e-77.
    IPB001939A    192-212
    IPB001939B    226-247
    IPB001939C    268-301
    IPB001939D    319-365


 ProDom Summary:  Protein Domain Search
Residues 227-374 are 31% similar to a (F54B3.3 PROTEIN) protein domain (PD094582 which is seen in Q20748_CAEEL.

Residues 225-435 are 27% similar to a (CELL DIVISION PROTEIN, PUTATIVE) protein domain (PD185276 which is seen in O83350_TREPA.

Residues 427-602 are 49% similar to a (PROTEIN CELL DIVISION ZINC METALLOPROTEASE HOMOLOG) protein domain (PD002293 which is seen in O81076_ARATH.

Residues 297-346 are 66% similar to a (CELL DIVISION PROTEIN FTSH) protein domain (PD194210 which is seen in FTH2_HAEIN.

Residues 306-372 are 49% similar to a (PROTEIN CELL DIVISION ATP-BINDING) protein domain (PD094491 which is seen in FTSH_CHLVU.

Residues 172-408 are 64% similar to a (PROTEIN ATP-BINDING PROTEASE SUBUNIT REGULATORY HOMOLOG) protein domain (PD000092 which is seen in FTSH_HELFE.


Paralogs:  Local Blast Search
Residues 193-299 are 34% similar to PG0382, an ATP-dependent ClpX-related protease.

Pfam Summary:  Pfam Search
Residues 43 to 202 (E-value = 2.4e-32) place PG0041 in the FtsH_ext family which is described as FtsH Extracellular (PF06480)
Residues 228 to 416 (E-value = 7e-94) place PG0041 in the AAA family which is described as ATPase family associated with various cellular activities (AAA) (PF00004)
Residues 422 to 626 (E-value = 8e-95) place PG0041 in the Peptidase_M41 family which is described as Peptidase family M41 (PF01434)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
18  
35
transmembrane  
19  
35
transmembrane  
135  
151
transmembrane  
138  
155
coil-coil  
591  
621
non-globular  
636  
673

Top PDB Hits:
-72% similar to PDB:2CE7 EDTA TREATED (E_value = 2.8E_125);
-72% similar to PDB:2CEA WILDTYPE (E_value = 2.8E_125);
-68% similar to PDB:2DHR Whole cytosolic region of ATP-dependent metalloprotease FtsH (G399L) (E_value = 8.0E_120);
-79% similar to PDB:1LV7 Crystal Structure of the AAA domain of FtsH (E_value = 3.9E_90);
-75% similar to PDB:1IY2 Crystal structure of the FtsH ATPase domain from Thermus thermophilus (E_value = 1.3E_82);

Gene Protein Sequence:
MANNGNNKKPGKPMRFNPIWLYAPVFLILATLFFVDRDITSQKELSWNEF
QNIAKKQAFTDIVVNRKENTLKARVDPAKVDSVFKKGDIPSFQDRGNISD
YYINTQIPSVDKFSDFYDQNQITAKVKYEDSKFSFTSILITWGPLILLLV
FWFWMMRRMSGGGGGSGGGGGVFNVGKSKAKLYDKTNIHVTFSDVAGLHE
AKQEVEEIVHFLKNPSKYTELGGKIPKGALLVGPPGTGKTLLAKAVAGEA
HVPFFSLSGSDFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAVGRA
RGKGNNFSGNDERENTLNQLLTEMDGFGSNSGVIILAATNRADVLDSALL
RAGRFDRQIYVDLPDLNDRKEIFLVHLKPLKTDKSVDVEFLSRQTPGFSG
ADIANVCNEAALIAARSNKNFVDKEDFMNAVDRIVGGLEKKNKITTEEER
RSIAIHEAGHATISWMLRYANPLVKVTIVPRGKALGAAWYLPEERQITTT
EALQDQLCALLGGRAAEDLFLGRVSTGAANDLERVTKLAYAMVTYYGMSD
KLPNINYYEMQNDGWNLTKPYSDTTAEVIDAEVNRIISEQYERAKSILRE
HEAGHHELADLLLKREVILADDVERIFGKRPWASRTEELLGLNAPATATE
ETTADAEAPIPVEGEEKTEEHPE

Gene Nucleotide Sequence:  Sequence Viewer
ATGGCGAACAATGGGAATAACAAGAAACCCGGAAAACCGATGCGTTTCAA
CCCGATATGGCTCTATGCCCCTGTATTTCTGATATTGGCCACCCTCTTCT
TTGTCGATCGGGATATTACATCCCAGAAAGAACTCAGCTGGAATGAGTTT
CAGAATATCGCCAAAAAGCAAGCCTTTACGGACATCGTAGTCAATCGGAA
AGAGAACACTCTCAAGGCGAGGGTCGATCCGGCCAAGGTCGATTCGGTAT
TCAAGAAAGGCGACATCCCGTCCTTTCAGGACAGAGGAAATATCTCAGAC
TACTATATCAACACGCAAATTCCGTCGGTAGACAAATTCTCCGACTTCTA
CGACCAAAACCAAATTACGGCTAAGGTGAAGTATGAAGACAGCAAGTTCA
GCTTCACCTCTATCCTCATTACATGGGGCCCACTCATCCTTTTGCTCGTT
TTCTGGTTTTGGATGATGCGCCGAATGAGCGGCGGTGGAGGAGGAAGTGG
CGGTGGAGGCGGCGTCTTCAACGTAGGCAAATCCAAAGCCAAGCTCTATG
ACAAGACCAATATCCACGTTACATTCAGCGATGTAGCAGGTCTGCATGAA
GCCAAGCAGGAAGTGGAAGAGATCGTTCACTTCCTCAAGAATCCATCCAA
ATACACCGAACTGGGAGGAAAGATCCCTAAAGGAGCTTTGCTCGTAGGAC
CTCCGGGAACGGGTAAGACTCTTCTGGCCAAGGCAGTAGCCGGAGAGGCG
CACGTTCCTTTCTTCTCTTTGTCCGGTTCGGACTTCGTAGAGATGTTTGT
CGGCGTAGGAGCCTCTCGTGTTCGCGACCTTTTCAGACAAGCCAAGGAAA
AAGCCCCGTGTATCATCTTCATCGACGAGATCGACGCCGTAGGGCGTGCT
CGCGGCAAGGGCAACAACTTCTCCGGCAATGATGAGCGTGAGAATACACT
CAACCAGTTGCTTACCGAAATGGATGGCTTCGGCTCCAATAGCGGTGTGA
TCATTCTGGCTGCTACCAACCGTGCCGATGTGCTGGATAGTGCCTTGCTG
CGTGCCGGACGTTTCGACCGCCAGATCTATGTAGATCTTCCCGACCTGAA
TGACCGCAAAGAGATCTTCCTTGTACACCTTAAACCGTTGAAGACCGACA
AAAGTGTAGATGTGGAATTCCTGTCGCGCCAAACACCCGGCTTCTCCGGA
GCAGACATTGCCAACGTTTGCAACGAAGCGGCGTTGATCGCCGCGCGAAG
CAATAAAAACTTCGTAGACAAGGAAGACTTCATGAATGCCGTGGATCGGA
TCGTAGGCGGATTGGAGAAAAAGAACAAAATCACCACAGAGGAGGAGCGT
CGCAGCATCGCCATCCACGAAGCCGGACACGCTACTATCAGTTGGATGCT
ACGCTATGCCAATCCTCTCGTCAAAGTCACTATCGTACCGCGAGGCAAGG
CACTCGGTGCCGCCTGGTATCTGCCGGAAGAGCGTCAGATCACGACAACG
GAGGCTCTGCAGGATCAGCTTTGCGCCCTTTTGGGTGGACGTGCCGCCGA
AGATCTCTTCCTCGGACGTGTATCGACCGGTGCAGCCAATGACTTGGAGC
GCGTTACGAAGTTGGCTTACGCCATGGTTACCTATTATGGCATGAGCGAC
AAGCTGCCCAACATCAACTACTACGAAATGCAAAACGACGGCTGGAATCT
GACAAAGCCGTACAGTGATACTACCGCCGAGGTTATCGATGCCGAAGTAA
ACAGAATAATATCCGAACAATACGAGCGCGCCAAAAGCATTCTGCGCGAG
CATGAAGCCGGGCACCACGAACTGGCAGATCTGTTGCTCAAGCGCGAAGT
GATTCTGGCTGATGACGTAGAGCGTATATTCGGCAAACGCCCCTGGGCTT
CGCGTACAGAAGAATTGCTTGGGCTGAATGCACCGGCTACCGCCACAGAA
GAGACAACAGCGGATGCCGAAGCACCCATTCCCGTTGAGGGAGAAGAGAA
GACGGAAGAACATCCCGAA

Los Alamos National Laboratory • Est 1943

Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration
Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy