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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0055 IGR0057 IGR0052 IGR0058 IGR0054 IGR0056 IGR0053 PG0057 lpxA, - PG0059 pyrF,ura3, - PG0062 czcB, - PG0056 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 czcA, - PG0055 PG0057 lpxA, - PG0059 pyrF,ura3, - PG0062 czcB, - PG0056 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 czcA, - PG0055 PG0057 lpxA, - PG0059 pyrF,ura3, - PG0062 czcB, - PG0056 PG0064 fabZ, - PG0060 PG0058 czcA, - PG0055 firA,lpxD, - PG0061 firA,lpxD, - PG0061 prfA, - PG0063


LANL Gene ID: PG0059

GenBank Locus Tag: PG0070

DNA Molecule Name:
1  

GenBank ID:
34539941

Gene Name:
lpxA  

Definition:
UDP-N-acetylglucosamine acyltransferase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
84824

Gene Stop:
84033

Gene Length:
792

Molecular Weight*:
28649

pI*:
6.20

Net Charge*:
-5.39

EC:
2.3.1.129  

Functional Class:
Cell envelope; Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides  

Gene Ontology:
Biological process
  GO:0008610    lipid biosynthetic process

Molecular function
  GO:0008415    acyltransferase activity
  GO:0008780    acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity


Pathway: pathway table
Nucleotide Metabolism; Aminosugarsmetabolism

Comment:
In E.coli, LPXA is involved in the first step
of lipid A biosynthesis; lipid A is an
endotoxin that anchors LPS to the outer
membrane. An Xray crystallographic structure
is known for this molecule.

TIGR ID: PG0070

Proteomic Data: Proteomic Data Search
TIGR Annotationacyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine acyltransferase, GroupC (cell envelope)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG00700.660.00037-0.120.210.721.4e-06-0.120.21
LANLPG00591.580.921.651.00
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Numerous hits in gapped BLAST to acyl carrier protiens, e.g. residues 6-260 are 60% similar to LPXA_AQUAE (AE000698).

This sequence is similar to CT531 of Chlamydia trachomatis.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539941|ref|NP_904420.1|  UDP-N-acetylglucosamine acyltr...   527   e-148
 gi|150008716|ref|YP_001303459.1|  acyl-(acyl-carrier-protein...   362   1e-98
 gi|154490825|ref|ZP_02030766.1|  hypothetical protein PARMER...   357   3e-97
 gi|150002707|ref|YP_001297451.1|  acyl-[acyl-carrier-protein...   285   1e-75
 gi|110637446|ref|YP_677653.1|  acyl-[acyl-carrier-protein]--...   284   3e-75
 gi|126647786|ref|ZP_01720291.1|  UDP-N-acetylglucosamine acy...   281   2e-74
 gi|53712198|ref|YP_098190.1|  UDP-N-acetylglucosamine acyltr...   276   8e-73
 gi|156107449|gb|EDO09194.1|  hypothetical protein BACOVA_050...   272   1e-71
 gi|29349613|ref|NP_813116.1|  UDP-N-acetylglucosamine acyltr...   272   2e-71
 gi|153807525|ref|ZP_01960193.1|  hypothetical protein BACCAC...   272   2e-71


InterPro Summary:  InterProScan

InterPro
IPR001451
Repeat
Bacterial transferase hexapeptide repeat
PF00132 [16-33]T 199.999965421631 PF00132 Hexapep[34-51]T 230.000061930563 PF00132 Hexapep[52-69]T 3.40000019100937 PF00132 Hexapep[82-99]T 53000.023946794696 PF00132 Hexapep[106-123]T 1299.9999583543402 PF00132 Hexapep[130-147]T 22999.9935665378 PF00132 Hexapep[148-165]T 1000.0 PF00132 Hexapep Hexapep
InterPro
IPR010137
Family
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
PIRSF000456 [4-261]T 9.30000000000139E-104 PIRSF000456 UDP-GlcNAc_acltr UDP-GlcNAc_acltr
TIGR01852 [7-259]T 0.0 TIGR01852 lipid_A_lpxA lipid_A_lpxA
InterPro
IPR011004
Domain
Trimeric LpxA-like
SSF51161 [5-260]T 2.6E-64 SSF51161 Trimer_LpxA_like Trimer_LpxA_like
noIPR
unintegrated
unintegrated
G3DSA:2.160.10.10 [4-195]T 5.30001985225048E-53 G3DSA:2.160.10.10 G3DSA:2.160.10.10 G3DSA:2.160.10.10


COGS Summary:  COGS Search
BeTs to 6 clades of COG1043
COG name: Acyl-[acyl carrier protein]--UDP-N-acetylglucosamine O-acyltransferase
Functional Class: M
The phylogenetic pattern of COG1043 is -----q-ce--huj----inx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 194-260 are 44% similar to a (ACYLTRANSFERASE TRANSFERASE) protein domain (PD007405 which is seen in LPXA_AQUAE.

Residues 5-91 are 41% similar to a (ACYLTRANSFERASE TRANSFERASE) protein domain (PD004893 which is seen in Q9ZJL7_BBBBB.

Residues 92-184 are 45% similar to a (TRANSFERASE ACYLTRANSFERASE PROTEIN REPEAT BIOSYNTHESIS) protein domain (PD000343 which is seen in LPXA_AQUAE.


Paralogs:  Local Blast Search
Residues 7-179 are 25% similar to PG0061, a predicted UDP-3-O-(R-3-hydoxymyristoyl)-glucosamine-N-acyltransferase.

Pfam Summary:  Pfam Search
No significant hits to the Pfam 11.0 database

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
-58% similar to PDB:1LXA UDP N-ACETYLGLUCOSAMINE ACYLTRANSFERASE (E_value = 3.3E_48);
-58% similar to PDB:2AQ9 Structure of E. coli LpxA with a bound peptide that is competitive with acyl-ACP (E_value = 3.3E_48);
-57% similar to PDB:1J2Z Crystal structure of UDP-N-acetylglucosamine acyltransferase (E_value = 7.2E_43);
-45% similar to PDB:2IU8 CHLAMYDIA TRACHOMATIS LPXD WITH 25MM UDPGLCNAC (COMPLEX I) (E_value = 4.1E_14);
-45% similar to PDB:2IU9 CHLAMYDIA TRACHOMATIS LPXD WITH 100MM UDPGLCNAC (COMPLEX II) (E_value = 4.1E_14);

Gene Protein Sequence:
MMSETKISPLAWVDPHAEIGVGVEIGPFAVVEAGAKIGDGSILHPHAVVR
YGSTLGKGCEIHPNAVIGGVPQDLKFQGEDTTAILGDYTIVRECATVNRG
TASRGTTVVGSHCLLMAYSHIAHDCVLGDHIIVGNASQIAGEVEIDDHAI
ISGGVLIHQFVRISQHVMIQGGSRLSKDIPPYVLVGRDPLVYCGINIVGL
RRRNFTNEQIFLINDIYRTLYQRGLNNSDAIDIIQQEYADCHEKELILDF
IKSSKRGIVRGTME

Gene Nucleotide Sequence:  Sequence Viewer
ATGATGTCAGAGACAAAAATCAGTCCGTTGGCATGGGTGGATCCGCATGC
CGAGATAGGAGTAGGTGTTGAGATAGGTCCATTTGCCGTCGTGGAAGCCG
GAGCTAAGATTGGTGATGGTTCCATTTTGCATCCTCATGCTGTGGTTCGT
TATGGATCGACGCTCGGTAAGGGTTGCGAAATCCATCCGAATGCAGTAAT
CGGAGGAGTACCGCAGGATCTGAAGTTCCAAGGTGAAGATACCACTGCTA
TCCTCGGCGATTATACCATCGTACGCGAATGTGCTACCGTCAATAGAGGC
ACCGCCTCTCGTGGCACTACCGTAGTGGGCAGTCATTGTCTGCTGATGGC
TTATAGTCATATAGCCCATGACTGCGTATTGGGCGATCATATCATTGTCG
GCAATGCTTCACAGATAGCCGGCGAAGTAGAAATAGACGACCATGCCATC
ATCAGCGGCGGAGTGTTGATACACCAGTTCGTACGCATCTCTCAGCATGT
GATGATCCAAGGTGGCTCCCGCCTCTCGAAGGACATCCCTCCGTATGTAC
TGGTGGGACGGGATCCTCTCGTTTATTGCGGAATCAACATCGTAGGTCTG
CGCCGCCGAAATTTCACCAACGAGCAGATTTTCCTGATCAATGATATTTA
CCGCACACTTTACCAAAGAGGACTGAATAATTCCGATGCGATCGATATTA
TCCAGCAGGAGTACGCCGATTGTCATGAGAAGGAACTTATATTAGACTTT
ATCAAGTCTTCCAAACGCGGCATTGTCCGCGGAACCATGGAG

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