Basic Search | Intermediate Search | Advanced Search | Gene Image Map |  Sequence Search |  Protein Motif Search |  Repeat Search |  DNA/RNA Motif Search |  Home

Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0055 IGR0057 IGR0058 IGR0054 IGR0056 IGR0053 PG0057 lpxA, - PG0059 pyrF,ura3, - PG0062 czcB, - PG0056 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 PG0057 lpxA, - PG0059 pyrF,ura3, - PG0062 czcB, - PG0056 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 PG0057 lpxA, - PG0059 pyrF,ura3, - PG0062 czcB, - PG0056 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 PG0065 firA,lpxD, - PG0061 firA,lpxD, - PG0061 PG0065


LANL Gene ID: PG0060

GenBank Locus Tag: PG0071

DNA Molecule Name:
1  

GenBank ID:
34539942

Gene Name:
fabZ  

Definition:
UDP-3-O-acyl-GlcNAc deacetylase/(3R)-hydroxymyristoyl-acp-dehydratase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
86209

Gene Stop:
84824

Gene Length:
1386

Molecular Weight*:
51680

pI*:
5.40

Net Charge*:
-10.02

EC:
3.5.1.-  

Functional Class:
Fatty acid and phospholipid metabolism  

Gene Ontology:
Biological process
  GO:0006633    fatty acid biosynthetic process
  GO:0009245    lipid A biosynthetic process

Cellular component
  GO:0005737    cytoplasm

Molecular function
  GO:0008759    UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity
  GO:0016836    hydro-lyase activity


Pathway: pathway table
cell envelope; surface polysaccharides,lipopolysaccharides and antigens fatty acid and phospholipid metabolism

Comment:
Results of gapped BLAST showed similarity to the first 300 amino acids or to the last 140 amino acids. There was no complete match to the whole protein sequence. This may be a fused protein in comparison with other genomes, or a stop/start codon may have been missed.

In E.coli, the (3R)-hydroxymyristoyl-ACP
dehydratase is involved in lipid A
biosynthesis. See Mohan et al., J.Biol.Chem.
269:32896 (1994).

TIGR ID: PG0071

Proteomic Data: Proteomic Data Search
TIGR AnnotationUDP-3-O-acyl-GlcNAc deacetylase, GroupC (cell envelope)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG00710.330.010.100.180.953.1e-140.100.18
LANLPG00601.261.071.941.30
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Numerous hits in gapped BLAST to UDP-3-O-acyl-N-acetylglucosamine deacetylase sequences (N-terminal) and to beta-hydroxyacyl-ACP dehydratases (C-terminal, see comments), e.g. residues 1-297 are 36% similar to the enzyme from Pseudomonas aeruginosa (U67855), residues 1-297 are 32% similar to (AE003920) of Xylella fastidiosa. Residues 319-454 are 52% similar to (AC007168) putative beta-hydroxyacyl-ACP dehydratase of Arabidopsis thaliana.

This sequence is similar to CT532, CT533 and BT4206.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539942|ref|NP_904421.1|  UDP-3-O-acyl-GlcNAc deacetylas...   924   0.0  
 gi|154490826|ref|ZP_02030767.1|  hypothetical protein PARMER...   665   0.0  
 gi|150008715|ref|YP_001303458.1|  UDP-3-O-[3-hydroxymyristoy...   665   0.0  
 gi|156107450|gb|EDO09195.1|  hypothetical protein BACOVA_050...   639   0.0  
 gi|29349614|ref|NP_813117.1|  UDP-3-O-[3-hydroxymyristoyl] N...   636   0.0  
 gi|153807526|ref|ZP_01960194.1|  hypothetical protein BACCAC...   635   e-180
 gi|156859251|gb|EDO52682.1|  hypothetical protein BACUNI_034...   635   e-180
 gi|150002706|ref|YP_001297450.1|  UDP-3-O-[3-hydroxymyristoy...   630   e-179
 gi|53712199|ref|YP_098191.1|  UDP-3-O-[3-hydroxymyristoyl] N...   627   e-178
 gi|88805698|ref|ZP_01121217.1|  UDP-3-O-[3-hydroxymyristoyl]...   462   e-128


InterPro Summary:  InterProScan

InterPro
IPR004463
Family
UDP-3-0-acyl N-acetylglucosamine deacetylase
PD011499 [3-132]T 0.0 PD011499 Lipid_A_LpxC Lipid_A_LpxC
PF03331 [1-302]T 1.2000011745814001E-60 PF03331 LpxC LpxC
TIGR00325 [1-328]T 2.17059078725666E-53 TIGR00325 lpxC lpxC
InterPro
IPR010084
Domain
Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ
TIGR01750 [319-457]T 3.54326183527155E-67 TIGR01750 fabZ fabZ
InterPro
IPR013114
Domain
Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase, FabA/FabZ
PF07977 [328-452]T 4.30000170645879E-46 PF07977 FabA FabA
noIPR
unintegrated
unintegrated
G3DSA:3.10.129.10 [320-462]T 4.1999977133960203E-44 G3DSA:3.10.129.10 G3DSA:3.10.129.10 G3DSA:3.10.129.10
G3DSA:3.30.1700.10 [128-305]T 1.99999636034536E-37 G3DSA:3.30.1700.10 G3DSA:3.30.1700.10 G3DSA:3.30.1700.10
G3DSA:3.30.230.20 [2-126]T 4.30000170645879E-37 G3DSA:3.30.230.20 G3DSA:3.30.230.20 G3DSA:3.30.230.20
SSF54211 [2-125]T 1.0999999999999999E-38 SSF54211 SSF54211[135-316]T 3.0999999999999997E-40 SSF54211 SSF54211 SSF54211
SSF54637 [318-462]T 6.599999999999999E-41 SSF54637 SSF54637 SSF54637


COGS Summary:  COGS Search
BeTs to 6 clades of COG0774
COG name: UDP-3-0-acyl-N-acetylglucosamine deacetylase
Functional Class: M
The phylogenetic pattern of COG0774 is -----q-ce--huj----inx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** PF01377 (Thioester dehydrase) with a combined E-value of 9.3e-43.
    PF01377A    329-340
    PF01377B    348-387
    PF01377C    404-446


 ProDom Summary:  Protein Domain Search
Residues 6-297 are 35% similar to a (DEACETYLASE UDP-3-O-3-HYDROXYMYRISTOYL) protein domain (PD011499 which is seen in P97050_PSEAE.

Residues 318-443 are 40% similar to a (PROTEIN DEHYDRATASE CARRIER) protein domain (PD005671 which is seen in O97376_TOXGO.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
Residues 1 to 302 (E-value = 1.1e-63) place PG0060 in the LpxC family which is described as UDP-3-O-acyl N-acetylglycosamine deacetylase (PF03331)
Residues 362 to 451 (E-value = 3.7e-16) place PG0060 in the 4HBT family which is described as Thioesterase superfamily (PF03061)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
-49% similar to PDB:2IER Crystal Structure of Aquifex aeolicus LpxC Complexed with Uridine 5'-Diphosphate (E_value = 1.4E_27);
-49% similar to PDB:2IES Crystal Structure of Aquifex aeolicus LpxC Complexed with Pyrophosphate (E_value = 1.4E_27);
-49% similar to PDB:2J65 STRUCTURE OF LPXC FROM AQUIFEX AEOLICUS IN COMPLEX WITH UDP (E_value = 1.4E_27);
-49% similar to PDB:2O3Z X-ray crystal structure of LpxC complexed with 3-heptyloxybenzoate (E_value = 1.4E_27);
-49% similar to PDB:1P42 Crystal structure of Aquifex aeolicus LpxC Deacetylase (Zinc-Inhibited Form) (E_value = 1.8E_27);

Gene Protein Sequence:
MQKQQTLKDKFCLQGKGLHTGLDIHITFCPAPEESGYKIKRVDLEGQPVI
DAIADNVHSTRRGTVLKKGEVSVSTIEHAMAALYALGVDNCLIEVDAPEF
PILDGSAEPYVSEIKRVGLVEQETPRDYYIIKKRMEVSDPESNSKLILLP
DDEFTVDVHIAFPSKVLSNQFASLETLSDFEEQIAGARTFVFVREVQMLL
EANLIKGGDLDNALVIYDEPLAQDRLDALSDMMGVERKQVNELGYINNKP
LIYDNEPARHKLLDVLGDLALIGKYIRGRIIATCPGHSINNKMARLIRKE
IKQNEAQAPVYNPNKEPIMDINRIKELLPHRYPFLLVDKIIEVGPDYIVG
VKSVSGNEPFFPGHFPGEPVMPGVLQVEAMAQVGGLLVLNTLTEPSSYST
YFLMIDKVKFRRKVVPGDTLVFKLRMISEIRRGVANMRGLAFVGEQLACE
AEFMAQIIQNKE

Gene Nucleotide Sequence:  Sequence Viewer
ATGCAAAAACAACAAACACTGAAAGATAAGTTCTGTCTGCAAGGCAAAGG
CCTACACACCGGACTGGATATTCATATTACTTTCTGTCCTGCTCCGGAGG
AATCCGGATATAAAATCAAGCGTGTCGATCTGGAGGGACAACCCGTTATA
GACGCCATCGCAGACAATGTGCACAGTACTCGTCGCGGTACGGTGCTGAA
AAAAGGCGAAGTTTCCGTCAGCACGATAGAACACGCCATGGCAGCTCTCT
ATGCCCTTGGCGTAGACAACTGCTTGATCGAGGTGGATGCACCCGAATTT
CCCATCTTGGATGGTAGCGCGGAACCCTATGTGAGCGAAATCAAGCGCGT
CGGCTTGGTGGAACAGGAAACACCACGCGATTATTATATAATAAAAAAAC
GCATGGAGGTGTCCGATCCGGAAAGCAATTCGAAGTTGATCCTGCTGCCG
GATGACGAATTTACAGTAGATGTACACATCGCTTTCCCCAGCAAGGTGCT
TAGCAATCAATTCGCTTCTTTGGAAACATTGTCGGATTTCGAAGAGCAGA
TTGCAGGTGCTCGCACCTTTGTTTTCGTACGGGAGGTGCAGATGCTTCTG
GAGGCCAATCTGATCAAAGGCGGCGATTTGGACAATGCTTTGGTGATCTA
CGACGAGCCATTGGCACAAGATCGACTCGATGCCCTTAGTGATATGATGG
GAGTGGAGCGGAAGCAGGTCAATGAATTGGGGTACATCAACAACAAGCCT
CTAATCTACGACAATGAACCGGCACGCCACAAGCTCCTCGACGTATTGGG
CGATCTGGCTTTGATCGGAAAATATATTCGCGGACGCATTATTGCCACCT
GTCCGGGGCACAGCATCAACAACAAGATGGCGCGCCTGATTCGCAAAGAA
ATCAAACAGAACGAAGCACAGGCCCCTGTCTACAATCCGAACAAAGAGCC
GATCATGGACATCAATCGGATCAAAGAGCTTCTACCCCACCGCTATCCGT
TCCTATTGGTCGATAAGATCATCGAAGTAGGGCCGGACTATATAGTCGGA
GTAAAGAGCGTTTCCGGCAACGAACCATTTTTCCCCGGTCACTTCCCTGG
TGAGCCTGTGATGCCGGGAGTCCTGCAAGTAGAGGCAATGGCGCAGGTGG
GAGGGCTCTTGGTACTCAATACCCTGACCGAGCCTTCGAGCTACTCCACA
TACTTCCTTATGATCGACAAAGTGAAATTCCGCCGTAAAGTGGTACCCGG
CGACACTTTGGTGTTCAAGCTCAGAATGATTTCCGAGATACGCAGAGGAG
TCGCTAATATGCGCGGCTTAGCTTTCGTAGGAGAGCAGTTAGCCTGTGAA
GCTGAATTTATGGCACAGATAATCCAGAATAAAGAA

Los Alamos National Laboratory • Est 1943

Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration
Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy