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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0055 IGR0057 IGR0058 IGR0054 IGR0056 IGR0059 IGR0053 PG0057 PG0066 lpxA, - PG0059 pyrF,ura3, - PG0062 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 PG0057 PG0066 lpxA, - PG0059 pyrF,ura3, - PG0062 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 PG0057 lpxA, - PG0059 pyrF,ura3, - PG0062 prfA, - PG0063 PG0064 fabZ, - PG0060 PG0058 PG0065 firA,lpxD, - PG0061 firA,lpxD, - PG0061 PG0066 PG0065


LANL Gene ID: PG0061

GenBank Locus Tag: PG0072

DNA Molecule Name:
1  

GenBank ID:
34539943

Gene Name:
firA  lpxD  

Definition:
UDP-3-O-(R-3-hydoxymyristoyl)-glucosamine-N-acyltransferase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
87237

Gene Stop:
86191

Gene Length:
1047

Molecular Weight*:
37390

pI*:
6.20

Net Charge*:
-6.29

EC:
2.3.1.-  

Functional Class:
Cell envelope; Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides  

Gene Ontology:
Biological process
  GO:0009245    lipid A biosynthetic process

Molecular function
  GO:0008415    acyltransferase activity
  GO:0016747    transferase activity, transferring groups other than amino-acyl groups


Pathway: pathway table
Aminosugars metabolism
Butanoate metabolism
D-Arginine and D-ornithine metabolism
Glycerolipid metabolism
Histidine metabolism
Lipopolysaccharide biosynthesis
Lysine biosynthesis
Lysine degradation
Phenylalanine metabolism
Tyrosine metabolism
Valine, leucine and isoleucine degradation

Comment:
TIGR ID: PG0072

Proteomic Data: Proteomic Data Search
TIGR AnnotationUDP-3-O-3-hydroxymyristoyl glucosamine N-acyltransferase, GroupC (cell envelope)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG00720.750.0660.090.234.918.9e-090.090.23
LANLPG00611.681.0730.03664.82
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Numerous hits in gapped BLAST to UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferases, e.g. residues 3-342 are 54% similar to the enzyme from N.meningiditis (AL162752).

This sequence is orthologous to CT243 and BT4207.



 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539943|ref|NP_904422.1|  UDP-3-O-[3-hydroxymyristoyl] g...   709   0.0  
 gi|150008714|ref|YP_001303457.1|  UDP-3-O-[3-hydroxymyristoy...   442   e-122
 gi|153807527|ref|ZP_01960195.1|  hypothetical protein BACCAC...   422   e-116
 gi|154490827|ref|ZP_02030768.1|  hypothetical protein PARMER...   414   e-114
 gi|29349615|ref|NP_813118.1|  UDP-3-O-[3-hydroxymyristoyl] g...   412   e-113
 gi|150002705|ref|YP_001297449.1|  UDP-3-O-[3-hydroxymyristoy...   409   e-112
 gi|156107451|gb|EDO09196.1|  hypothetical protein BACOVA_050...   406   e-112
 gi|156859250|gb|EDO52681.1|  hypothetical protein BACUNI_034...   398   e-109
 gi|53712200|ref|YP_098192.1|  UDP-3-O-[3-hydroxymyristoyl] g...   396   e-108
 gi|60680380|ref|YP_210524.1|  putative UDP-3-O-[3-hydroxymyr...   395   e-108


InterPro Summary:  InterProScan

InterPro
IPR001451
Repeat
Bacterial transferase hexapeptide repeat
PF00132 [110-127]T 21999.990098070703 PF00132 Hexapep[128-145]T 1299.9999583543402 PF00132 Hexapep[146-163]T 129.999995835434 PF00132 Hexapep[164-181]T 3.90000022371571 PF00132 Hexapep[201-218]T 110.000029896149 PF00132 Hexapep[223-240]T 11000.0029896149 PF00132 Hexapep[241-258]T 38999.9861795218 PF00132 Hexapep[259-276]T 70999.9884211148 PF00132 Hexapep[277-294]T 36999.984480221705 PF00132 Hexapep Hexapep
InterPro
IPR007691
Family
UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, LpxD
PF04613 [20-91]T 3.8000069709354496E-20 PF04613 LpxD LpxD
TIGR01853 [7-330]T 3.89788636264699E-128 TIGR01853 lipid_A_lpxD lipid_A_lpxD
InterPro
IPR011004
Domain
Trimeric LpxA-like
SSF51161 [39-292]T 6.9E-54 SSF51161 Trimer_LpxA_like Trimer_LpxA_like
noIPR
unintegrated
unintegrated
G3DSA:2.160.10.10 [116-312]T 3.89999861795218E-51 G3DSA:2.160.10.10 G3DSA:2.160.10.10 G3DSA:2.160.10.10


COGS Summary:  COGS Search
BeTs to 6 clades of COG1044
COG name: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
Functional Class: M
The phylogenetic pattern of COG1044 is -----q-ce--huj----inx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** IPB001451 (Bacterial transferase hexapeptide repeat) with a combined E-value of 8.3e-07.
    IPB001451A    161-180
    IPB001451B    210-220


 ProDom Summary:  Protein Domain Search
Residues 183-218 are 66% similar to a (ACYLTRANSFERASE TRANSFERASE) protein domain (PD088500 which is seen in Q44630_BRUAB.

Residues 200-309 are 47% similar to a (TRANSFERASE ACYLTRANSFERASE PROTEIN REPEAT BIOSYNTHESIS) protein domain (PD000343 which is seen in Q9Z8N6_BBBBB.


Paralogs:  Local Blast Search
Residues 119-296 share 25% identity with PG0059, UDP-N-acetylglucosamine acyltransferase.

Pfam Summary:  Pfam Search
Residues 3 to 103 (E-value = 5.8e-07) place PG0061 in the LpxD family which is described as UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase, LpxD (PF04613)

 Structural Feature(s):
Feature Type  Start  Stop
non-globular  
100  
186
non-globular  
243  
349

Top PDB Hits:
-55% similar to PDB:2IU8 CHLAMYDIA TRACHOMATIS LPXD WITH 25MM UDPGLCNAC (COMPLEX I) (E_value = 1.7E_53);
-55% similar to PDB:2IU9 CHLAMYDIA TRACHOMATIS LPXD WITH 100MM UDPGLCNAC (COMPLEX II) (E_value = 1.7E_53);
-55% similar to PDB:2IUA C. TRACHOMATIS LPXD (E_value = 1.7E_53);
-42% similar to PDB:1LXA UDP N-ACETYLGLUCOSAMINE ACYLTRANSFERASE (E_value = 2.1E_11);
-42% similar to PDB:2AQ9 Structure of E. coli LpxA with a bound peptide that is competitive with acyl-ACP (E_value = 2.1E_11);

Gene Protein Sequence:
MEFTAQQIADYLHGSVEGNPKVRLHDFAKIEEGRSGCLSFLANAKYEHYL
YQTQSDAVLVNQDFEPRESVKTTLIRVPNAYTALAQLMQLVDSMKPQRKG
VDSTAFVHPSVILPDDCYVGAFAYVSEGASLGTGCSLYPHVYVGSGVSVG
EGTILYPHVTVYDGCSIGSRCVIHSGAVIGADGFGFAPNAEGYSKIPQLG
NVIIEDDVEIGANTCIDRAVMDSTIIHRGVKLDNLVQIAHNCSVGSHTVF
AAQVGMAGSSHVGEWCQFGGQVGLSGHIKVGDRVSLGGQTGLLSNVKSGS
TLLGSPGMPLRDMLRASVIFPKLPDMSLRIEQLEKEISELKEICKNNKH

Gene Nucleotide Sequence:  Sequence Viewer
ATGGAATTTACAGCCCAACAGATAGCTGACTACCTCCACGGTTCTGTGGA
GGGCAATCCCAAGGTGCGACTGCACGACTTTGCCAAGATAGAGGAAGGTC
GTAGTGGCTGTCTCTCGTTTTTAGCCAATGCTAAGTACGAACACTATTTG
TACCAAACACAATCCGATGCAGTACTCGTCAATCAGGACTTCGAACCGCG
CGAATCGGTGAAGACGACCCTGATACGAGTTCCCAATGCCTATACAGCAT
TGGCGCAACTAATGCAGTTGGTGGACAGCATGAAGCCCCAAAGGAAGGGA
GTCGATTCCACGGCTTTCGTTCATCCATCGGTGATACTGCCGGATGATTG
CTATGTAGGAGCTTTTGCCTACGTGTCGGAAGGGGCATCGCTGGGTACAG
GCTGTAGCTTGTATCCGCATGTTTATGTAGGTAGCGGTGTGTCTGTTGGA
GAGGGGACGATCCTCTACCCCCATGTGACGGTCTATGATGGCTGTTCCAT
CGGTTCGCGTTGCGTGATTCATTCGGGAGCCGTTATCGGAGCGGATGGAT
TCGGTTTTGCTCCGAATGCCGAAGGCTACAGCAAGATTCCGCAACTGGGG
AACGTTATCATTGAAGATGATGTCGAAATCGGTGCCAACACTTGTATCGA
CAGGGCTGTTATGGATTCAACGATCATACATCGCGGAGTAAAGCTAGACA
ACCTTGTACAGATTGCTCACAACTGTTCTGTCGGAAGTCACACGGTTTTT
GCTGCCCAAGTCGGCATGGCCGGTTCGTCCCATGTGGGCGAATGGTGCCA
GTTCGGAGGGCAGGTGGGTTTGTCCGGGCACATCAAAGTGGGAGACAGGG
TCAGTTTAGGAGGACAGACCGGCTTGTTGAGCAACGTCAAATCCGGTTCC
ACGCTTCTCGGTTCGCCCGGGATGCCGCTACGGGATATGCTGCGAGCATC
GGTCATCTTTCCCAAACTCCCGGATATGAGCCTTCGCATCGAACAGCTGG
AAAAAGAAATATCAGAACTAAAAGAAATATGCAAAAACAACAAACAC

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