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Record: 1 of 1 MiniMap

LANL Gene ID: PG0074GenBank Locus Tag: PG0086DNA Molecule Name: 1 GenBank ID: 34539956Gene Name:Definition: DEAD BOX-related helicaseCellular Location: Cytoplasm [Evidence]Gene Start: 102449Gene Stop: 100638Gene Length: 1812Molecular Weight*: 68984pI*: 8.40Net Charge*: 6.44EC: Functional Class: Transcription; Transcription factors Gene Ontology: Molecular function GO:0003676 nucleic acid binding GO:0004386 helicase activity GO:0005524 ATP binding GO:0008026 ATP-dependent helicase activityPathway: pathway tableComment: TIGR ID: PG0086Proteomic Data: Proteomic Data Search

TIGR Annotation		ATP-dependent RNA helicase, DEAD/DEAH box family, GroupR (transcription)
ORF #		PG-PP/PG-PPC1				PG-PP/PG-PPC2
		Spectral Count		Peptide Intensity		Spectral Count		Peptide Intensity
		Pro	qVal	Pro	qVal	Pro	qVal	Pro	qVal
TIGR	PG0086	0.85	0.0067	0.92	0.028	0.96	0.0003	0.92	0.028
LANL	PG0074	1.80		1.89		1.95		0.91
Consensus		SP1		IP1		SP2		IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer Blast Summary: PSI-Blast Search Numerous hits in gapped BLAST to DEAD Box family sequences, e.g. residues 1-542 are 39% similar to the helicase from B halodurans (AP001515). This sequence is similar to BT3303. Top Blast Hits: Updated monthly Click here to view the entire PsiBlast results. gi|34539956|ref|NP_904435.1| ATP-dependent RNA helicase, DE... 1184 0.0 gi|154490038|ref|ZP_02030299.1| hypothetical protein PARMER... 754 0.0 gi|150009493|ref|YP_001304236.1| ATP-dependent RNA helicase... 737 0.0 gi|156861952|gb|EDO55383.1| hypothetical protein BACUNI_010... 694 0.0 gi|29348712|ref|NP_812215.1| ATP-dependent RNA helicase [Ba... 680 0.0 gi|53711460|ref|YP_097452.1| ATP-dependent RNA helicase [Ba... 677 0.0 gi|60679730|ref|YP_209874.1| putative RNA helicase dead-box... 677 0.0 gi|156111625|gb|EDO13370.1| hypothetical protein BACOVA_009... 669 0.0 gi|150003861|ref|YP_001298605.1| ATP-dependent RNA helicase... 664 0.0 gi|153807208|ref|ZP_01959876.1| hypothetical protein BACCAC... 660 0.0InterPro Summary: InterProScan InterPro IPR000629 Domain ATP-dependent helicase, DEAD-box PS00039 DEAD_ATP_HELICASE InterPro IPR001650 Domain Helicase, C-terminal PF00271 Helicase_C SM00490 HELICc PS51194 HELICASE_CTER InterPro IPR005580 Domain DbpA, RNA-binding PF03880 DbpA InterPro IPR011545 Domain DEAD/DEAH box helicase, N-terminal PF00270 DEAD InterPro IPR014001 Domain DEAD-like helicases, N-terminal SM00487 DEXDc InterPro IPR014014 Domain DEAD-box RNA helicase Q motif PS51195 Q_MOTIF InterPro IPR014021 Domain Helicase superfamily 1 and 2 ATP-binding PS51192 HELICASE_ATP_BIND_1 noIPR unintegrated unintegrated G3DSA:3.40.50.300 G3DSA:3.40.50.300 PTHR10967 PTHR10967 PTHR10967:SF51 PTHR10967:SF51 SSF54928 SSF54928 COGS Summary: COGS Search BeTs to 13 clades of COG0513 COG name: Superfamily II DNA and RNA helicases Functional Class: L,K,J The phylogenetic pattern of COG0513 is amTkYq-CEBRHujGPol--x Number of proteins in this genome belonging to this COG is 2 Blocks Summary: Blocks Search ***** IPB000629 (ATP-dependent helicase, DEAD-box subfamily) with a combined E-value of 2.2e-70. IPB000629A 22-60 IPB000629B 75-87 IPB000629C 147-170 IPB000629D 179-207 IPB000629E 295-345 ProDom Summary: Protein Domain Search Residues 12-104 are 49% similar to a (HELICASE ATP-BINDING RNA-BINDING PROTEIN RNA) protein domain (PD000085 which is seen in DEAD_KLEPN. Residues 217-355 are 43% similar to a (HELICASE PROTEIN POLYPROTEIN ATP-BINDING RNA RNA-BINDING) protein domain (PD000033 which is seen in Y669_METJA. Residues 106-207 are 52% similar to a (HELICASE ATP-BINDING RNA-BINDING RNA PROTEIN) protein domain (PD000276 which is seen in P96614_BACSU. Residues 245-328 are 30% similar to a (PROTEIN ORF PUTATIVE KINASE) protein domain (PD000146 which is seen in O97290_PLAFA. Paralogs: Local Blast Search PG0074 is similar to PG1835, PG0381 and PG1597, all sequences with predicted helicase activity. Residues 4-390 are 35% similar to PG1835, residues 21-362 are 23% similar to PG0381 and residues 21-345 are 22% similar to PG1597. Pfam Summary: Pfam Search Residues 15 to 220 (E-value = 1.6e-75) place PG0074 in the DEAD family which is described as DEAD/DEAH box helicase (PF00270) Residues 261 to 337 (E-value = 1.3e-32) place PG0074 in the Helicase_C family which is described as Helicase conserved C-terminal domain (PF00271) Residues 457 to 543 (E-value = 2.4e-09) place PG0074 in the DbpA family which is described as DbpA RNA binding domain (PF03880) Structural Feature(s): Feature Type Start Stop coil-coil 252 282 coil-coil 415 457 non-globular 509 604 Top PDB Hits: -68% similar to PDB:1HV8 CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII (E_value = 2.9E_81); -58% similar to PDB:1FUU YEAST INITIATION FACTOR 4A (E_value = 3.3E_61); -57% similar to PDB:2HXY Crystal structure of human apo-eIF4AIII (E_value = 8.2E_60); -57% similar to PDB:2HYI Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA (E_value = 8.2E_60); -57% similar to PDB:2J0Q THE CRYSTAL STRUCTURE OF THE EXON JUNCTION COMPLEX AT 3.2 A RESOLUTION (E_value = 8.2E_60); Gene Protein Sequence: MKTFEELGIAKPFLKAIAELGFEQPMPVQEEVIPYLLGEDIDLIALAQTG TGKTAAFGLPLLQKIDLSGGRPQALVLCPTRELCLQIADDLNSYSKYLSD VHILPVYGGTPIDAQIRALRRGVQIVVATPGRLLDLMRREAVSLSDIHTV VLDEADEMLNMGFAEDLEQILADIPSERHLLLFSATMPKEISKIATSYMK DPKEIVIGNKNEGNANIKHLYYMVSARHKYLALKRIADYYPNIYGIVFCR TRKETQEIADQLIQDGYNADSLHGDLSQAQRDYVMQKFRVRNLQLLVATD VAARGLDVNDLTHVIHFGLPDDVESYTHRSGRTARAGKTGLSIAICHVKE KGKIKNIERTMQKQIERARIPSGAEICEKQLFNLADRIERVDIENTTAID SVLNEVNKKLEWIEKDELIRRVMALEFNRMLDYYQRAEDVEEVVERKKDE VSGDRKNRKRGAGEAEEGMTRLFINFGKMDQMFPNRLIELINRCIPGRVN IGRIDLMPRFAFFDVDEFEAKNVVETLNRYEVDGRRIHVEYADTKKDYAG GKKGGDKNFSARRKDFDGRRSENPTSRKNKGRKEKPTEGRFYDKFDSKQR KRRK Gene Nucleotide Sequence: Sequence Viewer ATGAAAACATTTGAAGAATTAGGCATTGCCAAACCGTTTTTGAAAGCAAT TGCCGAATTGGGCTTCGAACAGCCCATGCCTGTGCAGGAAGAAGTAATAC CCTATCTCCTCGGAGAGGACATCGACCTGATCGCTTTGGCACAGACCGGC ACAGGCAAGACTGCCGCTTTCGGCCTTCCGCTGCTCCAGAAAATTGATTT GTCGGGAGGACGACCGCAGGCTTTGGTACTCTGCCCGACTCGCGAACTGT GTCTACAGATAGCAGACGACTTGAACAGCTATTCCAAGTATTTGTCCGAT GTGCACATCCTACCCGTTTACGGAGGTACGCCTATAGATGCGCAGATCAG AGCCTTGCGCCGTGGTGTGCAAATCGTCGTGGCAACGCCCGGCCGTCTCC TCGACCTGATGCGCCGTGAGGCCGTCAGCCTGTCGGACATTCATACGGTA GTATTGGATGAAGCGGATGAAATGCTCAATATGGGCTTTGCAGAAGATCT GGAGCAAATCCTCGCAGATATTCCATCCGAACGCCATCTGCTATTGTTTT CGGCTACGATGCCCAAAGAGATCTCCAAGATCGCCACTTCGTACATGAAG GACCCCAAGGAAATTGTTATCGGCAATAAGAATGAGGGCAATGCCAATAT CAAGCATCTATACTACATGGTCTCGGCGCGCCATAAATATCTTGCGCTAA AGCGAATTGCCGACTACTATCCCAATATCTATGGTATTGTTTTCTGTCGC ACCCGTAAGGAAACGCAGGAGATTGCCGATCAACTGATTCAAGACGGCTA TAACGCGGATTCTCTTCATGGCGATCTCAGTCAGGCTCAGCGCGACTATG TGATGCAGAAGTTTCGCGTCCGCAACCTTCAACTGCTCGTTGCCACCGAT GTGGCTGCCCGCGGATTGGATGTGAACGATCTGACCCATGTGATTCACTT CGGTCTTCCCGATGATGTAGAGAGCTACACCCACCGCAGCGGTCGTACAG CTCGTGCAGGGAAAACAGGTCTTTCGATCGCCATCTGCCATGTCAAGGAG AAAGGCAAAATCAAGAATATAGAGCGCACGATGCAGAAGCAGATCGAACG TGCACGCATCCCTTCCGGAGCAGAGATATGCGAAAAGCAACTGTTCAATC TGGCCGATCGTATAGAACGTGTGGATATAGAGAACACAACGGCCATCGAC TCTGTCTTGAACGAAGTGAACAAGAAGCTCGAATGGATTGAAAAAGACGA ATTGATTCGTCGGGTAATGGCATTGGAGTTCAATCGCATGCTCGACTATT ACCAACGTGCCGAAGACGTGGAAGAAGTGGTCGAGAGAAAAAAAGACGAG GTTTCGGGAGATCGTAAAAACCGAAAACGAGGAGCCGGCGAAGCAGAAGA AGGGATGACCCGTCTGTTCATCAATTTTGGCAAGATGGATCAAATGTTTC CCAACCGACTGATCGAGCTGATCAACCGTTGTATCCCGGGGCGTGTCAAT ATCGGACGGATCGACCTGATGCCCCGATTCGCTTTCTTCGATGTAGACGA GTTCGAAGCCAAGAACGTAGTAGAAACCCTCAATCGGTACGAAGTGGATG GTCGCCGCATCCATGTAGAGTATGCGGATACCAAGAAAGACTATGCCGGG GGGAAAAAAGGCGGAGATAAGAACTTTTCCGCTCGTCGCAAAGATTTCGA CGGGCGACGTAGTGAAAATCCCACTTCACGCAAAAACAAAGGTCGCAAAG AGAAACCGACGGAAGGACGTTTCTACGACAAGTTCGACTCCAAACAGCGC AAGCGTCGGAAG Los Alamos National Laboratory • Est 1943 Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy

`Feature Type`	`Start`	`Stop`
`coil-coil`	`252`	`282`
`coil-coil`	`415`	`457`
`non-globular`	`509`	`604`