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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0073 IGR0074 IGR0072 IGR0071 IGR0069 IGR0070 IGR0075 PG0082 PG0081 PG0080 PG0083 mutS, - PG0084 PG0078 dps, - PG0079 PG0077 PG0082 PG0081 PG0080 PG0083 mutS, - PG0084 PG0078 dps, - PG0079 PG0077 Type: tandem, Name: tan33 - 241 Type: tandem, Name: tan34 - 242 Type: tandem, Name: tan84 - 292 PG0082 PG0081 PG0080 PG0083 mutS, - PG0084 dps, - PG0079 PG0077 PG0078


LANL Gene ID: PG0082

GenBank Locus Tag: PG0093

DNA Molecule Name:
1  

GenBank ID:
34539962

Gene Name:


Definition:
conserved hypothetical protein (36 kDa antigen)

Cellular Location:
Inner membrane, Periplasm, Cytoplasm [Evidence]

Gene Start:
109728

Gene Stop:
108736

Gene Length:
993

Molecular Weight*:
36000

pI*:
8.90

Net Charge*:
1.27

EC:
 

Functional Class:
Unknown; Conserved hypothetical  

Gene Ontology:

Pathway: pathway table

Primary Evidence:
Ross BC, Czajkowski L, Hocking D, Margetts M, Webb E, Rothel L, Patterson M, Agius C, Camuglia S, Reynolds E, Littlejohn T, Gaeta B, Ng A, Kuczek ES, Mattick JS, Gearing D, Barr IG, Identification of vaccine candidate antigens from a genomic analysis of Porphyromonas gingivalis, Vaccine 2001 Jul 20;19(30): 4135-42, PMID: 11457538.

Comment:
Ross, et al.(2001) select PG0082 as one of fifteen vaccine candidate antigens, based on in vitro and in vivo laboratory studies of 120 candidates screened by bioinformatics techniques from the whole W50 genome.

TIGR ID: PG0093

Proteomic Data: Proteomic Data Search
TIGR AnnotationHlyD family secretion protein, GroupS (transport and binding proteins)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG00931.590.0110.870.0574.623e-070.870.057
LANLPG00823.021.8324.57634.92
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0082 is identical to the previously sequenced gb|AAD39494.1 in GenBANK, a predicted 36 kDa antigen. Several hits in gapped BLAST to conserved hypothetical proteins, e.g. residues 15-330 are 38% similar to gb|AAF94761.1| from V.cholerae.

This sequence is similar to BT3904.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539962|ref|NP_904441.1|  HlyD family secretion protein ...   538   e-151
 gi|156111383|gb|EDO13128.1|  hypothetical protein BACOVA_011...   410   e-113
 gi|29349312|ref|NP_812815.1|  hypothetical protein BT_3904 [...   410   e-113
 gi|153809120|ref|ZP_01961788.1|  hypothetical protein BACCAC...   409   e-113
 gi|53715269|ref|YP_101261.1|  hypothetical protein BF3985 [B...   404   e-111
 gi|156859683|gb|EDO53114.1|  hypothetical protein BACUNI_031...   382   e-104
 gi|150002858|ref|YP_001297602.1|  hypothetical protein BVU_0...   346   1e-93
 gi|154493624|ref|ZP_02032944.1|  hypothetical protein PARMER...   322   2e-86
 gi|150006810|ref|YP_001301553.1|  hypothetical protein BDI_0...   322   3e-86
 gi|154493601|ref|ZP_02032921.1|  hypothetical protein PARMER...   259   2e-67


InterPro Summary:  InterProScan

InterPro
IPR000089
Domain
Biotin/lipoyl attachment
PF00364 [50-78]T 0.0039000007589703803 PF00364 Biotin_lipoyl Biotin_lipoyl
InterPro
IPR011053
Domain
Single hybrid motif
SSF51230 [33-120]T 4.0E-7 SSF51230 Hybrid_motif Hybrid_motif
noIPR
unintegrated
unintegrated
SSF111369 [163-330]T 4.6E-5 SSF111369 SSF111369 SSF111369


COGS Summary:  COGS Search
BeTs to 5 clades of COG0845
COG name: Membrane permeases, predicted cation efflux pumps
Functional Class: R
The phylogenetic pattern of COG0845 is -----QvCEB-hUJ--ol--x
Number of proteins in this genome belonging to this COG is 6
Blocks Summary:  Blocks Search
***** IPB002215 (HlyD family secretion protein) with a combined E-value of 2.6e-07.
    IPB002215A    44-80
    IPB002215B    209-243


 ProDom Summary:  Protein Domain Search
Residues 139-329 are 36% similar to a (ANTIGEN TRANSMEMBRANE PUTATIVE) protein domain (PD107678 which is seen in Q9ZJD1_BBBBB.

Residues 41-138 are 32% similar to a (PROTEIN MEMBRANE TRANSMEMBRANE INNER) protein domain (PD001100 which is seen in AN36_HELPY.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
No significant hits to the Pfam 11.0 database

 Structural Feature(s):
Feature Type  Start  Stop
uncleavable signal  
1  
26
transmembrane  
14  
30
transmembrane  
14  
31
coil-coil  
83  
114
coil-coil  
151  
182

Top PDB Hits:
-57% similar to PDB:1O78 BIOTIN CARBOXYL CARRIER DOMAIN OF TRANSCARBOXYLASE (1.3S) [10-48] DELETION MUTANT (E_value = );
-64% similar to PDB:2D5D Structure of Biotin Carboxyl Carrier Protein (74Val start) from Pyrococcus horikoshi OT3 Ligand Free Form II (E_value = );
-64% similar to PDB:2EVB Structure of Biotin Carboxyl Carrier Protein (74Val start) from Pyrococcus horikoshi OT3 Ligand Free Form I (E_value = );
-48% similar to PDB:1LAB THREE-DIMENSIONAL STRUCTURE OF THE LIPOYL DOMAIN FROM BACILLUS STEAROTHERMOPHILUS PYRUVATE DEHYDROGENASE MULTIENZYME COMPLEX (E_value = );
-48% similar to PDB:1LAC THREE-DIMENSIONAL STRUCTURE OF THE LIPOYL DOMAIN FROM BACILLUS STEAROTHERMOPHILUS PYRUVATE DEHYDROGENASE MULTIENZYME COMPLEX (E_value = );

Gene Protein Sequence:
MTDNKQRNIVFPAFLLLLGVIAVVTIVGFFMLRPAEEIIQGQIEVTEYRV
SSKVPGRIKELRVSEGQQVQAGDTLAVIEAPDVAAKMEQAKAAEAAAQAQ
NAKALKGARSEQIQAAYEMWQKAQAGVAIATKTHQRVQNLYDQGVVPAQK
LDEATAQRDAAIATQKAAEAQYNMARNGAEREDKLAASALVDRARGAVAE
VESYINETYLIAPRAGEVSEIFPKAGELVGTGAPIMNIAEMGDMWASFAV
REDFLSSMTMGAVLETVVPALNEEKVRFKITFIKNMGTYAAWKATKTTGQ
YDLKTFEVKATLADKDKAQKLRPGMSVIIRK

Gene Nucleotide Sequence:  Sequence Viewer
ATGACGGACAACAAACAACGTAATATCGTATTCCCGGCGTTTCTCCTCTT
GCTGGGAGTCATCGCAGTGGTGACGATCGTTGGTTTTTTCATGCTCAGAC
CGGCCGAGGAGATTATCCAAGGACAGATAGAAGTGACCGAATACCGAGTG
TCCAGCAAAGTGCCCGGGCGCATCAAGGAACTTAGGGTATCCGAGGGACA
GCAGGTGCAGGCCGGCGATACCCTCGCTGTCATCGAAGCCCCCGACGTAG
CGGCTAAGATGGAGCAGGCAAAGGCTGCCGAAGCAGCTGCACAGGCTCAG
AACGCCAAGGCTCTCAAAGGAGCACGCAGCGAACAGATACAGGCAGCCTA
TGAGATGTGGCAGAAAGCTCAGGCCGGCGTAGCCATAGCGACCAAGACAC
ACCAGCGCGTGCAGAACCTCTATGACCAGGGAGTGGTACCGGCTCAGAAG
TTGGACGAAGCCACTGCCCAGCGCGATGCGGCCATCGCTACGCAAAAAGC
GGCCGAAGCCCAGTACAATATGGCTCGCAACGGTGCCGAACGCGAAGACA
AGCTGGCAGCTTCTGCCCTCGTCGATAGAGCGAGAGGAGCCGTCGCCGAG
GTGGAGTCGTACATCAACGAAACCTACCTCATCGCCCCACGGGCAGGCGA
AGTGTCGGAGATATTCCCCAAAGCCGGCGAACTCGTAGGTACCGGCGCAC
CTATCATGAATATCGCCGAGATGGGCGATATGTGGGCCAGCTTTGCCGTT
CGTGAGGATTTCCTCAGCAGCATGACCATGGGAGCCGTTCTGGAGACTGT
GGTGCCGGCTCTGAATGAAGAAAAAGTACGCTTCAAGATCACATTCATCA
AGAACATGGGTACCTATGCTGCCTGGAAAGCGACCAAGACAACAGGGCAG
TACGACCTGAAGACCTTCGAGGTAAAGGCCACCCTTGCGGATAAAGACAA
GGCACAAAAGCTACGCCCGGGTATGTCCGTGATCATACGCAAG

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