Basic Search | Intermediate Search | Advanced Search | Gene Image Map |  Sequence Search |  Protein Motif Search |  Repeat Search |  DNA/RNA Motif Search |  Home

Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap tRNA-Ile-1 tRNA-Ala-1 rRNA-16S-1 rRNA-23S-1 IGR0077 IGR0076 IGR0078 IGR0075 IGR0079 PG0088 PG0085 pheT, - PG0086 mutS, - PG0084 PG0088 PG0085 pheT, - PG0086 mutS, - PG0084 PG0088 PG0085 pheT, - PG0086 mutS, - PG0084


LANL Gene ID: PG0086

GenBank Locus Tag: PG0099

DNA Molecule Name:
1  

GenBank ID:
34539967

Gene Name:
pheT  

Definition:
phenylalanyl-tRNA synthetase beta subunit

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
118376

Gene Stop:
115920

Gene Length:
2457

Molecular Weight*:
91266

pI*:
5.30

Net Charge*:
-22.68

EC:
 6.1.1.20  

Functional Class:
Translation; Aminoacyl-tRNA synthetases  

Gene Ontology:
Biological process
  GO:0006432    phenylalanyl-tRNA aminoacylation

Cellular component
  GO:0005737    cytoplasm

Molecular function
  GO:0000049    tRNA binding
  GO:0004826    phenylalanine-tRNA ligase activity
  GO:0005524    ATP binding


Pathway: pathway table
Amino Acid Metabolism; Phenylalanine, tyrosine andtryptophan biosynthesis
Metabolism of Macromolecules; Aminoacyl-tRNAbiosynthesis

Comment:
The phenylalanyl-tRNA synthetase is typically a heteroterameric
molecule consisting of two alpha and two beta chains.
TIGR ID: PG0099 The alpha subunit is PG1545.

Proteomic Data: Proteomic Data Search
TIGR Annotationphenylalanyl-tRNA synthetase, beta subunit, GroupN (protein synthesis)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG00991.638.4e-111.511.8e-090.450.00351.511.8e-09
LANLPG00863.102.851.371.18
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search

Numerous significant hits in gapped BLAST to phenylalanyl-tRNA synthetase beta subunit; e.g. residues 1-819 are 36% similar to Z75208 of B. subtilis, residues 1-819 are 36% similar to AE004702 of Pseudomonas aeruginosa, residues 1-819 are 34% similar to AP001517 of Bacillus halodurans.

This sequence is also orthologous to CT475 and to BT0626.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539967|ref|NP_904446.1|  phenylalanyl-tRNA synthetase b...  1684   0.0  
 gi|150007874|ref|YP_001302617.1|  phenylalanyl-tRNA syntheta...  1032   0.0  
 gi|154493685|ref|ZP_02033005.1|  hypothetical protein PARMER...  1023   0.0  
 gi|150005970|ref|YP_001300714.1|  phenylalanyl-tRNA syntheta...   995   0.0  
 gi|53713856|ref|YP_099848.1|  phenylalanyl-tRNA synthetase b...   986   0.0  
 gi|60682069|ref|YP_212213.1|  phenylalanyl-tRNA synthetase b...   986   0.0  
 gi|153806615|ref|ZP_01959283.1|  hypothetical protein BACCAC...   976   0.0  
 gi|156110009|gb|EDO11754.1|  hypothetical protein BACOVA_022...   974   0.0  
 gi|156860836|gb|EDO54267.1|  hypothetical protein BACUNI_022...   973   0.0  
 gi|29346036|ref|NP_809539.1|  phenylalanyl-tRNA synthetase b...   969   0.0


InterPro Summary:  InterProScan

InterPro
IPR002547
Domain
t-RNA-binding region
PF01588 [48-152]T 9.10000425645606E-25 PF01588 tRNA_bind tRNA_bind
PS50886 [42-154]T 0.0 PS50886 TRBD TRBD
InterPro
IPR004532
Family
Bacterial phenylalanyl-tRNA synthetase, beta subunit
TIGR00472 [1-819]T 0.0 TIGR00472 pheT_bact pheT_bact
InterPro
IPR005121
Domain
Ferredoxin-fold anticodon-binding
G3DSA:3.30.70.380 [728-813]T 5.2000008900327595E-25 G3DSA:3.30.70.380 Fdx_AntiC_bd Fdx_AntiC_bd
PF03147 [726-819]T 1.89999859865843E-26 PF03147 FDX-ACB FDX-ACB
SSF54991 [720-819]T 2.1E-32 SSF54991 Fdx_AntiC_bd Fdx_AntiC_bd
InterPro
IPR005146
Domain
B3/4
PF03483 [224-398]T 4.80002938964668E-98 PF03483 B3_4 B3_4
InterPro
IPR005147
Domain
tRNA synthetase, B5
G3DSA:3.30.56.20 [414-488]T 1.29999924468183E-12 G3DSA:3.30.56.20 B5 B5
PF03484 [414-483]T 3.50000235434301E-15 PF03484 B5 B5
InterPro
IPR009061
Domain
Putative DNA binding
SSF46955 [413-487]T 9.5E-18 SSF46955 Putativ_DNA_bind Putativ_DNA_bind
InterPro
IPR012340
Domain
Nucleic acid-binding, OB-fold
G3DSA:2.40.50.140 [41-160]T 5.59999989416987E-33 G3DSA:2.40.50.140 OB_NA_bd_sub OB_NA_bd_sub
noIPR
unintegrated
unintegrated
G3DSA:3.30.930.10 [503-709]T 9.00000407957216E-28 G3DSA:3.30.930.10 G3DSA:3.30.930.10 G3DSA:3.30.930.10
G3DSA:3.50.40.10 [202-413]T 8.500030248090479E-73 G3DSA:3.50.40.10 G3DSA:3.50.40.10 G3DSA:3.50.40.10
PTHR10947 [1-819]T 0.0 PTHR10947 PTHR10947 PTHR10947
SSF50249 [38-187]T 1.8E-47 SSF50249 Nucleic_acid_OB Nucleic_acid_OB
SSF55681 [479-712]T 3.3999999999999996E-36 SSF55681 SSF55681 SSF55681
SSF56037 [206-412]T 5.2E-72 SSF56037 SSF56037 SSF56037


COGS Summary:  COGS Search
BeTs to 17 clades of COG0072
COG name: Phenylalanyl-tRNA synthetase beta subunit
Functional Class: J
The phylogenetic pattern of COG0072 is amtkYqvcebrhujgpolinx
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
***** PF01588 (Putative tRNA binding domain) with a combined E-value of 3.4e-08.
    PF01588A    62-73
    PF01588B    110-127
***** DM01195 (METHIONINE--TRNA LIGASE) with a combined E-value of 3.6e-06.
    DM01195A    57-65
    DM01195B    110-127


 ProDom Summary:  Protein Domain Search
Residues 155-260 are 34% similar to a (PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN) protein domain (PD217334 which is seen in SYFB_RICPR.

Residues 165-706 are 35% similar to a (SYNTHETASE PHENYLALANYL-TRNA AMINOACYL-TRNA BETA CHAIN) protein domain (PD003388 which is seen in O67620_AQUAE.

Residues 1-147 are 35% similar to a (SYNTHETASE PHENYLALANYL-TRNA BETA) protein domain (PD009777 which is seen in SYFB_MYCTU.

Residues 725-819 are 40% similar to a (SYNTHETASE AMINOACYL-TRNA) protein domain (PD006935 which is seen in SYFB_BACSU.

Residues 49-150 are 49% similar to a (SYNTHETASE AMINOACYL-TRNA PROTEIN LIGASE BIOSYNTHESIS) protein domain (PD002123 which is seen in SYFB_ECOLI.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
Residues 48 to 152 (E-value = 1.3e-26) place PG0086 in the tRNA_bind family which is described as Putative tRNA binding domain (PF01588)
Residues 162 to 307 (E-value = 1.7e-50) place PG0086 in the B3_4 family which is described as B3/4 domain (PF03483)
Residues 414 to 483 (E-value = 1.7e-18) place PG0086 in the B5 family which is described as tRNA synthetase B5 domain (PF03484)
Residues 726 to 819 (E-value = 3.9e-28) place PG0086 in the FDX-ACB family which is described as Ferredoxin-fold anticodon binding domain (PF03147)

 Structural Feature(s):
Feature Type  Start  Stop
non-globular  
1  
90
non-globular  
690  
780
coil-coil  
787  
819

Top PDB Hits:
-48% similar to PDB:1B70 PHENYLALANYL TRNA SYNTHETASE COMPLEXED WITH PHENYLALANINE (E_value = 1.5E_91);
-48% similar to PDB:1B7Y PHENYLALANYL TRNA SYNTHETASE COMPLEXED WITH PHENYLALANINYL-ADENYLATE (E_value = 1.5E_91);
-48% similar to PDB:1EIY THE CRYSTAL STRUCTURE OF PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH COGNATE TRNAPHE (E_value = 1.5E_91);
-48% similar to PDB:1JJC Crystal structure at 2.6A resolution of phenylalanyl-tRNA synthetase complexed with phenylalanyl-adenylate in the presence of manganese (E_value = 1.5E_91);
-48% similar to PDB:1PYS PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS (E_value = 1.5E_91);

Gene Protein Sequence:
MNISYKWLLEYLPCTLSPQEIADTLTSIGLETGGVEEIETIRGGLRGLVI
GHVLTCEEHPNSDHLHITTVDVGADAPLQIVCGAPNVAAGQKVVVATVGT
TLYHGEEEFAIKKSKIRGVESFGMICSEVEIGVGSSNDGTIVLPSDAPVG
MPAAEYYHVESDYCIEVDITPNRVDATSHYGVARDLAASLKRNGVPAELK
LPEVNLPTDIIDSRIEVKVADATACPRYQGLVIRDITVGESPEWLRNRLQ
AIGLRPINNIVDITNYVLHEFGQPLHAFDLAFIKGDRVHVQTVAEGTPFV
TLDGVERKLTAEDLMICDSNGDPMCVAGVFGGLHSGVTEKTTDIFLESAN
FNPTMVRRTARRLGLNTDSSFRFERGLDPERTDWALRRAASLILEIAGGH
LGGMTDVYSNPLKPHLISLSFEKVNSVIGRTIEPEMVRSILNSLEIRISK
EEDGVMTLEVPRYRTDVTRDVDVIEEIMRIYGYNQVELTGYIRASLGHET
ETDRRYKWQTVVSEQLVGAGFNEILNNSLTAGSYYEGLKSHPREMAVELM
NPLSQELNCMRQTLLFGGLETLSHNLRRKHLSLYLFEWGKCYRFHAAKRT
DETPLAAYAEDDRLGIWICGQRVHNSWAHPEEPTSVFELKAVVEQVLCRV
GIETGAYTLKTADNDLYASAMEVKTRSGKLLGTFGTVSTELIKRFEIEQP
VYFAELLWDALMSESARYKLEARDLPRFPEVKRDLALLLDKAVSFAEIES
LARGCEKKLLRRVELFDVYEGKNLPAGKKSYAVSFFLRNDEKTLNDKQIE
AIMAKIRTTLEQKLGAQLR

Gene Nucleotide Sequence:  Sequence Viewer
ATGAATATCTCATACAAATGGCTCTTGGAGTACCTGCCCTGCACCCTCTC
TCCGCAAGAGATAGCCGACACGCTCACTTCCATCGGTCTGGAGACCGGCG
GTGTAGAAGAGATCGAAACGATTCGGGGAGGCTTGCGAGGTTTGGTCATC
GGCCATGTTCTGACTTGTGAGGAACACCCGAATTCGGATCATTTGCATAT
CACCACGGTAGATGTGGGGGCGGATGCGCCTTTGCAAATCGTCTGCGGTG
CGCCCAATGTGGCTGCAGGACAAAAGGTGGTAGTGGCAACGGTGGGCACC
ACGCTCTATCACGGAGAAGAAGAGTTTGCCATCAAGAAATCCAAGATTCG
CGGAGTGGAGAGTTTCGGAATGATTTGCTCCGAAGTGGAAATCGGTGTAG
GCTCGTCGAATGACGGGACTATCGTCCTGCCGAGCGATGCGCCCGTGGGG
ATGCCTGCCGCCGAGTACTACCATGTAGAAAGCGATTATTGCATAGAAGT
GGACATTACGCCCAACCGCGTGGATGCCACTTCGCACTATGGTGTGGCAC
GCGACTTGGCGGCCAGCCTGAAACGTAATGGTGTACCTGCGGAACTCAAA
CTGCCGGAGGTAAACCTGCCGACGGACATCATCGACAGTCGAATAGAAGT
GAAAGTGGCAGATGCTACGGCCTGCCCACGCTATCAGGGATTGGTGATCA
GAGATATTACGGTAGGAGAAAGCCCCGAATGGCTGCGCAACAGGTTGCAG
GCGATCGGCCTGCGTCCGATCAATAACATCGTGGATATTACGAATTACGT
CCTTCATGAATTTGGCCAACCCCTTCATGCTTTTGATTTGGCTTTTATCA
AGGGAGACCGAGTTCATGTGCAGACAGTGGCCGAGGGTACTCCGTTCGTC
ACACTGGATGGAGTGGAGCGTAAGCTGACGGCCGAGGATCTGATGATATG
CGATTCGAACGGCGATCCCATGTGTGTGGCGGGCGTTTTCGGTGGTCTTC
ACTCCGGTGTAACGGAAAAGACAACAGATATATTCCTCGAGAGTGCCAAT
TTCAACCCGACGATGGTACGCCGTACGGCTCGCCGATTGGGTCTCAATAC
GGACAGTTCTTTCCGCTTTGAGCGCGGATTGGATCCGGAGCGCACGGATT
GGGCATTGCGTCGTGCTGCTTCGCTTATATTAGAGATTGCAGGAGGCCAT
CTCGGTGGGATGACAGATGTTTATTCCAATCCTCTGAAACCGCACCTAAT
CAGTTTGTCTTTCGAGAAAGTGAACAGCGTGATAGGCAGAACGATAGAGC
CTGAGATGGTGAGGAGCATCCTCAACAGCCTTGAGATTCGTATCAGCAAA
GAAGAAGACGGGGTCATGACGTTGGAAGTGCCACGCTATCGTACGGATGT
GACCCGCGATGTGGATGTGATCGAAGAGATCATGCGCATCTATGGCTACA
ACCAAGTGGAGCTGACGGGCTATATCCGTGCCAGCCTGGGACACGAGACA
GAAACAGATCGTCGCTACAAATGGCAGACAGTCGTATCCGAACAATTGGT
CGGAGCCGGTTTCAATGAGATACTCAACAATTCGCTTACGGCCGGTTCTT
ATTACGAAGGTCTTAAGAGCCATCCGAGAGAGATGGCAGTGGAGCTGATG
AATCCGCTGAGTCAGGAGCTTAACTGCATGAGGCAGACTCTGCTATTCGG
AGGATTGGAGACGCTCAGCCACAACCTCCGTCGTAAGCATCTCTCCCTCT
ATCTCTTTGAATGGGGTAAATGCTATCGCTTCCATGCCGCAAAGCGCACG
GACGAGACTCCCCTTGCGGCCTATGCAGAGGACGATCGCCTCGGCATTTG
GATATGCGGACAACGGGTGCACAATAGCTGGGCACACCCCGAAGAACCGA
CTTCGGTATTTGAACTGAAAGCTGTGGTAGAACAGGTACTTTGCCGCGTA
GGGATCGAAACCGGAGCTTATACGCTGAAGACAGCTGACAATGATCTGTA
CGCATCTGCCATGGAGGTCAAGACTCGTTCGGGCAAACTGTTGGGTACAT
TCGGAACGGTATCAACGGAACTGATCAAACGCTTCGAAATCGAGCAACCC
GTTTACTTTGCCGAATTGCTTTGGGATGCCCTCATGAGCGAATCGGCCCG
ATACAAATTGGAGGCACGCGATCTGCCTCGCTTCCCCGAAGTAAAGAGGG
ATCTGGCATTGCTTTTGGACAAGGCTGTTTCCTTTGCCGAAATAGAATCA
CTGGCTCGTGGCTGTGAGAAGAAACTGCTGCGCAGGGTGGAACTATTCGA
TGTGTACGAGGGAAAGAACCTGCCTGCCGGAAAGAAGAGTTATGCAGTGA
GCTTCTTCCTGCGCAATGATGAGAAGACGCTTAACGACAAACAGATAGAG
GCTATCATGGCTAAGATACGGACGACTCTGGAGCAAAAGCTCGGTGCGCA
ACTCCGC

Los Alamos National Laboratory • Est 1943

Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration
Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy