Basic Search | Intermediate Search | Advanced Search | Gene Image Map |  Sequence Search |  Protein Motif Search |  Repeat Search |  DNA/RNA Motif Search |  Home

Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0083 IGR0084 IGR0085 IGR0086 IGR0082 IGR0081 IGR0080 PG0092 PG0098 PG0096 PG0093 wecC, - PG0094 PG0095 capK, - PG0097 top3,topB, - PG0091 PG0092 PG0098 PG0096 PG0093 wecC, - PG0094 PG0095 capK, - PG0097 top3,topB, - PG0091 Type: tandem, Name: tan29 - 237 PG0092 PG0098 PG0096 PG0093 wecC, - PG0094 PG0095 capK, - PG0097 top3,topB, - PG0091


LANL Gene ID: PG0094

GenBank Locus Tag: PG0108

DNA Molecule Name:
1  

Genomic Island ID:
2

GenBank ID:
34539974

 Gene Name:
wecC  

 Definition:
UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
130241

Gene Stop:
131446

Gene Length:
1206

Molecular Weight*:
44779

pI*:
6.00

Net Charge*:
-6.70

EC:
 

 Functional Class:
Cell envelope; Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides  

 Gene Ontology:
Biological process
  GO:0006118    electron transport

Molecular function
  GO:0016616    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
  GO:0051287    NAD binding


 Pathway: pathway table

 Comment:
TIGR ID: PG0108

 Proteomic Data: Proteomic Data Search
TIGR AnnotationUDP-N-acetyl-D-mannosaminuronic acid dehydrogenase, GroupC (cell envelope)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG01080.340.110.810.0320.240.180.810.032
LANLPG00941.271.751.180.98
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Numerous hits in gapped BLAST to UDP-ManNAc dehydrogenase sequences, e.g. residues 4-402 are 69% similar to the protein from Bacteroides fragili (gb|AAD56735.1). Residues 7-385 are 50% similar to the enzyme from E. coli (gb|AAG58982.1).

This sequence is similar to BT2945.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539974|ref|NP_904453.1|  UDP-N-acetyl-D-mannosaminuroni...   834   0.0  
 gi|63107057|emb|CAI94404.1|  hypothetical protein [Porphyrom...   812   0.0  
 gi|150006699|ref|YP_001301442.1|  putative UDP-ManNAc dehydr...   639   0.0  
 gi|154494117|ref|ZP_02033437.1|  hypothetical protein PARMER...   639   0.0  
 gi|60680299|ref|YP_210443.1|  putative UDP-ManNAc dehydrogen...   633   e-180
 gi|150003284|ref|YP_001298028.1|  putative UDP-ManNAc dehydr...   627   e-178
 gi|154494888|ref|ZP_02033893.1|  hypothetical protein PARMER...   612   e-173
 gi|150008212|ref|YP_001302955.1|  putative UDP-ManNAc dehydr...   610   e-173
 gi|5931974|gb|AAD56735.1|AF125164_8  UDP-ManNAc dehydrogenas...   609   e-173
 gi|53712391|ref|YP_098383.1|  UDP-ManNAc dehydrogenase [Bact...   607   e-172


InterPro Summary:  InterProScan

InterPro
IPR001732
Domain
UDP-glucose/GDP-mannose dehydrogenase, N-terminal
PF03721 [2-187]T 6.79998707392899E-57 PF03721 UDPG_MGDP_dh_N UDPG_MGDP_dh_N
InterPro
IPR008927
Domain
6-phosphogluconate dehydrogenase, C-terminal-like
SSF48179 [195-285]T 2.8000000000000002E-28 SSF48179 6DGDH_C_like 6DGDH_C_like
InterPro
IPR014026
Domain
UDP-glucose/GDP-mannose dehydrogenase, dimerisation
PF00984 [195-285]T 4.90001027782408E-33 PF00984 UDPG_MGDP_dh UDPG_MGDP_dh
InterPro
IPR014027
Domain
UDP-glucose/GDP-mannose dehydrogenase, C-terminal
PF03720 [311-402]T 1.89999859865843E-11 PF03720 UDPG_MGDP_dh_C UDPG_MGDP_dh_C
InterPro
IPR014028
Domain
UDP-glucose/GDP-mannose dehydrogenase, dimerisation and substrate-binding
PTHR11374 [155-402]T 3.99998544139379E-94 PTHR11374 UDPG_MGDP_DH_Creg UDPG_MGDP_DH_Creg
InterPro
IPR014360
Family
UDP-glucose/GDP-mannose dehydrogenase
PIRSF000124 [1-401]T 7.700000000000319E-34 PIRSF000124 UDPglc_GDPman_dh UDPglc_GDPman_dh
InterPro
IPR014687
Family
UDP-N-acetyl-D-mannosaminuronate dehydrogenase
PIRSF500136 [2-402]T 0.0 PIRSF500136 UDP_ManNAc_DH UDP_ManNAc_DH
noIPR
unintegrated
unintegrated
G3DSA:1.10.8.220 [195-278]T 1.6000024069598602E-17 G3DSA:1.10.8.220 G3DSA:1.10.8.220 G3DSA:1.10.8.220
G3DSA:3.40.50.1870 [290-400]T 5.299996575502399E-12 G3DSA:3.40.50.1870 G3DSA:3.40.50.1870 G3DSA:3.40.50.1870
G3DSA:3.40.50.720 [2-193]T 1.6000024069598601E-49 G3DSA:3.40.50.720 G3DSA:3.40.50.720 G3DSA:3.40.50.720
PTHR11374:SF4 [155-402]T 3.99998544139379E-94 PTHR11374:SF4 PTHR11374:SF4 PTHR11374:SF4
SSF51735 [2-201]T 1.2999999999999998E-45 SSF51735 SSF51735 SSF51735
SSF52413 [302-402]T 5.1E-17 SSF52413 SSF52413 SSF52413


COGS Summary:  COGS Search
BeTs to 4 clades of COG0677
COG name: UDP-N-acetyl-D-mannosaminuronate dehydrogenase
Functional Class: M
The phylogenetic pattern of COG0677 is -mtK----e------------
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 256-385 are 39% similar to a (DEHYDROGENASE OXIDOREDUCTASE NAD UDP-GLUCOSE) protein domain (PD001281 which is seen in WECC_ECOLI.

Residues 148-255 are 65% similar to a (DEHYDROGENASE OXIDOREDUCTASE NAD UDP-GLUCOSE) protein domain (PD001282 which is seen in EPSD_BURSO.

Residues 7-125 are 52% similar to a (DEHYDROGENASE OXIDOREDUCTASE NAD UDP-GLUCOSE) protein domain (PD001278 which is seen in WECC_ECOLI.


Paralogs:  Local Blast Search
Residues 3-327 are 32% similar to PG1125 and residues 7-387 are 24% similar to PG1026. Both PG1125 and PG1026 are associated with polysaccharide biosynthesis.

Pfam Summary:  Pfam Search
Residues 2 to 187 (E-value = 9.9e-60) place PG0094 in the UDPG_MGDP_dh_N family which is described as UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721)
Residues 195 to 285 (E-value = 4.7e-36) place PG0094 in the UDPG_MGDP_dh family which is described as UDP-glucose/GDP-mannose dehydrogenase family, central domain (PF00984)
Residues 311 to 402 (E-value = 3.8e-13) place PG0094 in the UDPG_MGDP_dh_C family which is described as UDP-glucose/GDP-mannose dehydrogenase family, UDP binding domain (PF03720)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  
uncleavable signal  
1  
21

Top PDB Hits:
-44% similar to PDB:2O3J Structure of Caenorhabditis Elegans UDP-Glucose Dehydrogenase (E_value = 1.5E_24);
-46% similar to PDB:1MFZ Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa (E_value = 2.5E_22);
-46% similar to PDB:1MUU 2.0 A crystal structure of GDP-mannose dehydrogenase (E_value = 2.5E_22);
-46% similar to PDB:1MV8 1.55 A crystal structure of a ternary complex of GDP-mannose dehydrogenase from Psuedomonas aeruginosa (E_value = 2.5E_22);
-55% similar to PDB:2P0V Crystal structure of BT3781 protein from Bacteroides thetaiotaomicron, Northeast Structural Genomics Target BtR58 (E_value = 2.5E_22);

Gene Protein Sequence:
MRKACFMGLGYIGLPTAIVTAKSGIEVLGVDINTNIVAKTNSGKLHFVEA
GMEDLLQEVIQKETFRASTTPEDSDVYLVVVPTPFKANHEPDITCVEAAT
RSILPLLKKGDLFIIESTSPIGTTEQMADLIYRERPELKDQLYMAYCPER
ILPGNVIYELEHNDRVIGGLSAEATERAKEFYAHFVKGTLHGTNARTAEL
CKLTENASRDVQIAFANELSFICDKAGINVWELIELANKHPRVNILQPGS
GVGGHCIAVDPYFISSAFPLESKLIAGAREINNYKAFWCAEKISKAIQDF
EIRHGHTPVVAMMGLAFKPDIDDLRESPAKYIATRVMQGLNKAEILIVEP
NISKHNTFKLTDYRDAYEQADIVAFLTAHTPFKFLEYRTDKVILDFCGVF
KK

Gene Nucleotide Sequence:  Sequence Viewer
ATGAGAAAAGCTTGTTTCATGGGCCTGGGGTATATAGGTCTGCCAACGGC
AATTGTAACTGCCAAAAGTGGAATAGAAGTATTAGGGGTAGATATAAACA
CCAATATCGTTGCAAAAACCAATTCAGGAAAATTGCACTTTGTGGAAGCA
GGTATGGAAGATCTTTTACAGGAAGTTATACAAAAAGAAACATTCCGTGC
TTCTACAACACCGGAGGATAGCGATGTTTATCTGGTTGTGGTGCCTACAC
CCTTCAAGGCCAATCATGAGCCGGACATTACATGTGTAGAGGCTGCAACA
AGATCAATACTTCCCTTGCTCAAGAAGGGCGATTTATTTATTATCGAATC
TACCTCTCCGATAGGAACAACAGAGCAGATGGCTGATCTAATCTATCGAG
AGCGTCCCGAACTTAAAGACCAATTATATATGGCTTACTGTCCGGAACGT
ATCCTTCCCGGTAATGTTATTTATGAACTGGAGCACAACGATCGGGTCAT
CGGGGGCTTAAGCGCAGAAGCGACCGAACGGGCAAAAGAGTTCTATGCTC
ATTTTGTTAAAGGAACCTTGCATGGAACTAATGCTCGTACAGCAGAACTC
TGCAAGCTTACAGAGAATGCTAGCCGAGATGTACAGATCGCGTTTGCCAA
CGAACTTTCTTTCATTTGTGACAAAGCCGGAATCAATGTGTGGGAACTTA
TCGAACTGGCCAATAAGCATCCAAGGGTGAATATTCTTCAGCCCGGTAGT
GGAGTAGGGGGGCATTGTATTGCGGTGGATCCGTATTTTATCTCTTCGGC
TTTCCCTCTGGAGAGTAAACTGATTGCCGGTGCTCGTGAAATCAATAACT
ACAAGGCTTTTTGGTGTGCCGAGAAAATAAGTAAGGCTATTCAAGACTTT
GAGATACGACATGGCCATACTCCAGTAGTAGCTATGATGGGACTGGCCTT
TAAACCGGACATCGATGATCTCAGGGAGTCGCCTGCAAAATACATTGCCA
CTCGAGTAATGCAGGGGTTGAACAAAGCCGAGATCTTGATTGTAGAACCC
AACATTTCGAAGCATAATACTTTCAAGCTAACGGACTACCGTGATGCTTA
TGAGCAGGCAGACATTGTAGCTTTCTTAACGGCTCATACTCCTTTCAAAT
TCCTCGAATACCGAACCGACAAAGTCATCCTCGACTTCTGTGGTGTCTTC
AAAAAA

Los Alamos National Laboratory • Est 1943

Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration
Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy