Basic Search | Intermediate Search | Advanced Search | Gene Image Map |  Sequence Search |  Protein Motif Search |  Repeat Search |  DNA/RNA Motif Search |  Home

Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0095 IGR0097 IGR0098 IGR0094 IGR0099 IGR0093 IGR0100 IGR0092 IGR0096 PG0109 PG0108 gpmA,pgmA, - PG0115 PG0110 PG0117 PG0116 PG0106 PG0107 PG0113 hemH,hemZ, - PG0112 PG0114 weeA, - PG0105 PG0109 PG0108 gpmA,pgmA, - PG0115 PG0110 PG0117 PG0116 PG0106 PG0107 PG0113 hemH,hemZ, - PG0112 PG0114 weeA, - PG0105 PG0108 gpmA,pgmA, - PG0115 PG0110 PG0117 PG0111 PG0111 PG0109 PG0116 PG0107 PG0113 hemH,hemZ, - PG0112 PG0114 weeA, - PG0105 PG0106


LANL Gene ID: PG0112

GenBank Locus Tag: PG0127

DNA Molecule Name:
1  

Genomic Island ID:
3

GenBank ID:
34539991

 Gene Name:
hemH  hemZ  

 Definition:
ferrochelatase

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
148690

Gene Stop:
149724

Gene Length:
1035

Molecular Weight*:
39548

pI*:
8.20

Net Charge*:
6.48

EC:
4.99.1.1  

 Functional Class:
Biosynthesis of cofactors, prosthetic groups and carriers; Heme, porphyrin and cobalamin  

 Gene Ontology:
Biological process
  GO:0006783    heme biosynthetic process

Molecular function
  GO:0004325    ferrochelatase activity


 Pathway: pathway table
Porphyrin and chlorophyll metabolism

 Primary Evidence:
Roper JM, Raux E, Brindley AA, Schubert HL, Gharbia SE, Shah HN, Warren MJ. The enigma of
cobalamin (Vitamin B12) biosynthesis in Porphyromonas gingivalis. Identification and characterization of a functional corrin pathway. J Biol Chem. 2000 Dec 22;275(51):40316-23. PMID: 11007789

 Comment:
MD pathway protoporphyrin + Fe(2+) = protoheme + 2 H(+). Pathway: last step in protoheme biosynthesis.

Roper et al. (2000) assign a role in cobalamin biosynthesis to this protein. See in this regard PG193 (cbiC), PG0192 (cbiET), PG0191 (cbiGF), PG0190 (cbiJD), PG1040 (cbiA), PG1038 (cbiP), PG1037 (cobD), PG1036 (cobD/cbiB), PG0631 (cobU), PG0632 (cobT), PG0633 (cobS), PG0434 (hemN/F), PG0438 (cbiL), PG0602 (cbiK), PG0193 (cbiH).

TIGR ID: PG0127

 Proteomic Data: Proteomic Data Search
TIGR Annotationferrochelatase, GroupB (biosynthesis of cofactors, prosthetic groups, and carriers)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG0127-1.250.11-3.160.0014-3.160.0014
LANLPG01120.420.11
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
This sequence is orthologous to CT485.

Numerous significant hits in gapped BLAST to ferrochelatases; e.g. residues 17-344 are 36% similar to AF314196 of Pseudomonas fluorescens, residues 17-339 are 37% similar to AE004879 of Pseudomonas aeruginosa, residues 14-345 are 36% similar to AL162754 of N. meningitidis.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34539991|ref|NP_904470.1|  ferrochelatase [Porphyromonas ...   706   0.0  
 gi|86131000|ref|ZP_01049599.1|  Ferrochelatase [Cellulophaga...   247   7e-64
 gi|120437544|ref|YP_863230.1|  ferrochelatase (protoheme fer...   242   3e-62
 gi|89889958|ref|ZP_01201469.1|  ferrochelatase [Flavobacteri...   238   4e-61
 gi|86133884|ref|ZP_01052466.1|  Ferrochelatase [Tenacibaculu...   236   1e-60
 gi|70732458|ref|YP_262220.1|  ferrochelatase [Pseudomonas fl...   236   2e-60
 gi|91215562|ref|ZP_01252533.1|  ferrochelatase [Psychroflexu...   234   5e-60
 gi|88802323|ref|ZP_01117850.1|  ferrochelatase [Polaribacter...   234   9e-60
 gi|150024160|ref|YP_001294986.1|  Ferrochelatase [Flavobacte...   231   4e-59
 gi|88711472|ref|ZP_01105560.1|  ferrochelatase [Flavobacteri...   230   1e-58


InterPro Summary:  InterProScan

InterPro
IPR001015
Family
Ferrochelatase
PD002792 [19-345]T 0.0 PD002792 Ferrochelatase Ferrochelatase
PTHR11108 [17-207]T 9.800002721303041E-42 PTHR11108 Ferrochelatase[234-345]T 9.800002721303041E-42 PTHR11108 Ferrochelatase Ferrochelatase
PF00762 [14-345]T 1.60000240695986E-51 PF00762 Ferrochelatase Ferrochelatase
TIGR00109 [10-344]T 1.4320649456858004E-53 TIGR00109 hemH hemH
noIPR
unintegrated
unintegrated
G3DSA:3.40.50.1400 [11-218]T 2.500009099162E-34 G3DSA:3.40.50.1400 G3DSA:3.40.50.1400 G3DSA:3.40.50.1400
SSF53800 [11-345]T 2.0000000000000003E-73 SSF53800 SSF53800 SSF53800


COGS Summary:  COGS Search
BeTs to 8 clades of COG0276
COG name: Protoheme ferro-lyase (ferrochelatase)
Functional Class: H
The phylogenetic pattern of COG0276 is ----yq-cebrhuj----inx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** IPB001015 (Ferrochelatase) with a combined E-value of 5.1e-19.
    IPB001015A    16-35
    IPB001015B    194-207
    IPB001015C    260-276
    IPB001015D    289-310


 ProDom Summary:  Protein Domain Search
Residues 17-330 are 35% similar to a (FERROCHELATASE HEME BIOSYNTHESIS LYASE) protein domain (PD002792 which is seen in HEMZ_ECOLI.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
Residues 14 to 345 (E-value = 1.4e-54) place PG0112 in the Ferrochelatase family which is described as Ferrochelatase (PF00762)

 Structural Feature(s):
Feature Type  Start  Stop
uncleavable signal  
1  
60
transmembrane  
46  
62

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MADRRRTDDNKGAREVVLLLNIGSPDSPEEKDVARYLNSFLTDRRIITLP
FLLRQLLVRGIITPFRKKSSAQKYRTVWDESTRSFPLISHTKAIARALAH
TGREVHVAMRYGKPAVADVLKELPHGRSLVVLPLFPHYAMSSYETAVEHC
KAEIRRLCPNLSFRVVQPFYAHEAYIRVLADNIRPYLTKPFDKLILSYHG
IPRDHLDKTTRQALNLRHPEGCCTEEDPTANVCYRYQTYRTTALIREALG
LAEEQVEQVFQSRVGHTEWLRPYLIERLSAWPQEETKRILIACPSFVCDC
LESLEEVADHGQSIFKKAGGADFTYIPCLNSGANWIDALRNILEE

Gene Nucleotide Sequence:  Sequence Viewer
ATGGCAGATCGTAGAAGAACCGATGACAATAAGGGTGCGAGAGAGGTGGT
ATTGCTGCTGAATATCGGCTCGCCGGACAGCCCTGAGGAGAAGGACGTGG
CGCGCTACCTCAATTCTTTTCTGACCGACAGGAGGATCATCACCTTGCCT
TTTCTCCTGAGACAGTTACTCGTGAGAGGTATCATCACTCCTTTCAGAAA
GAAAAGTTCGGCACAAAAATATCGGACCGTTTGGGACGAAAGCACCCGGT
CTTTCCCCCTCATATCGCATACGAAAGCCATAGCCCGGGCACTGGCACAT
ACGGGCAGAGAAGTCCATGTAGCCATGCGCTACGGCAAGCCGGCTGTGGC
CGATGTGCTGAAGGAGCTGCCACACGGCCGAAGCCTCGTCGTCCTCCCGC
TCTTCCCACACTACGCCATGAGCAGCTACGAGACGGCCGTGGAGCATTGC
AAGGCCGAAATCCGCCGCCTTTGTCCGAATCTTTCCTTCCGCGTCGTGCA
GCCTTTCTACGCACACGAAGCCTATATTCGTGTCCTTGCCGATAACATCA
GACCCTATCTGACGAAGCCTTTCGACAAGCTCATTCTTTCCTATCACGGC
ATTCCCCGCGATCATTTGGACAAGACCACCCGACAGGCTTTGAATCTCCG
GCATCCCGAAGGATGCTGCACGGAGGAGGATCCTACGGCAAACGTCTGCT
ACCGCTATCAGACTTACAGGACGACGGCTCTCATCCGCGAGGCACTTGGC
TTGGCCGAGGAGCAGGTGGAGCAGGTGTTCCAGTCACGTGTAGGCCATAC
CGAATGGCTGCGCCCCTATTTGATCGAACGACTGTCCGCTTGGCCGCAAG
AGGAAACAAAACGTATCCTCATAGCCTGCCCCTCCTTCGTGTGCGACTGT
CTGGAGAGCCTCGAAGAAGTAGCCGATCACGGACAAAGCATTTTCAAAAA
AGCAGGAGGTGCGGATTTCACTTACATCCCCTGTCTCAATAGCGGGGCGA
ATTGGATCGATGCTCTCCGAAACATTTTAGAGGAA

Los Alamos National Laboratory • Est 1943

Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration
Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy