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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0120 IGR0118 IGR0119 IGR0122 IGR0123 IGR0121 IGR0117 sigG,sigW, - PG0148 PG0147 ribD, - PG0142 pfk,pfpB, - PG0150 aspS, - PG0141 pepO, - PG0146 PG0145 hemK, - PG0143 sigG,sigW, - PG0148 PG0147 ribD, - PG0142 pfk,pfpB, - PG0150 aspS, - PG0141 pepO, - PG0146 PG0145 hemK, - PG0143 sigG,sigW, - PG0148 PG0147 ribD, - PG0142 pfk,pfpB, - PG0150 aspS, - PG0141 pepO, - PG0146 PG0145 hemK, - PG0143 PG0144 PG0144


LANL Gene ID: PG0146

GenBank Locus Tag: PG0159

DNA Molecule Name:
1  

GenBank ID:
34540021

Gene Name:
pepO  

Definition:
endothelin converting enzyme/neprilysin (PepO)

Cellular Location:
Cytoplasm, Outer membrane, Periplasm, Extracellular [Evidence]

Gene Start:
183254

Gene Stop:
181188

Gene Length:
2067

Molecular Weight*:
78828

pI*:
5.30

Net Charge*:
-12.75

EC:
3.4.24.11  

Functional Class:
Uncategorized  

Gene Ontology:
Biological process
  GO:0006508    proteolysis

Cellular component
  GO:0016020    membrane

Molecular function
  GO:0004245    neprilysin activity
  GO:0008237    metallopeptidase activity
  GO:0008270    zinc ion binding


Pathway: pathway table

Primary Evidence:
Awano,S., Ansai,T., Mochizuki,H., Yu,W., Tanzawa,K., Turner,A.J.
and Takehara,T.
Sequencing, expression and biochemical characterization of the
Porphyromonas gingivalis pepO gene encoding a protein homologous to human endothelin-converting enzyme
FEBS Lett. 460 (1), 139-144 (1999)
PubMed: 10571076


Comment:
TIGR ID: PG0159

Proteomic Data: Proteomic Data Search
TIGR Annotationendopeptidase PepO, GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG0159-0.365.4e-07-0.030.23-0.441.5e-15-0.030.23
LANLPG01460.780.980.741.14
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0146 is equivalent to the previously sequenced dbj|BAA24495, a predicted PepO protein. Numerous hits in gapped BLAST to metallopeptidases, specifically endothelin converting enzyme/neprilysin. Residues 6-689 are 36% similar to the matallopeptidase from Xylella fastidiosa (gb|AAF83386).

This sequence is similar to BT3811.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540021|ref|NP_904500.1|  endopeptidase PepO [Porphyromo...  1395   0.0  
 gi|2804580|dbj|BAA24495.1|  PepO [Porphyromonas gingivalis]      1389   0.0  
 gi|150008197|ref|YP_001302940.1|  putative endopeptidase [Pa...   716   0.0  
 gi|154493575|ref|ZP_02032895.1|  hypothetical protein PARMER...   698   0.0  
 gi|156109275|gb|EDO11020.1|  hypothetical protein BACOVA_036...   691   0.0  
 gi|60683355|ref|YP_213499.1|  putative endopeptidase [Bacter...   691   0.0  
 gi|53715386|ref|YP_101378.1|  putative endothelin-converting...   689   0.0  
 gi|153809039|ref|ZP_01961707.1|  hypothetical protein BACCAC...   679   0.0  
 gi|156860364|gb|EDO53795.1|  hypothetical protein BACUNI_024...   675   0.0  
 gi|150004576|ref|YP_001299320.1|  putative endothelin-conver...   644   0.0


InterPro Summary:  InterProScan

InterPro
IPR000718
Family
Peptidase M13, neprilysin
PR00786 [477-489]T 5.0E-24 PR00786 NEPRILYSIN[495-507]T 5.0E-24 PR00786 NEPRILYSIN[516-532]T 5.0E-24 PR00786 NEPRILYSIN[587-598]T 5.0E-24 PR00786 NEPRILYSIN NEPRILYSIN
PTHR11733 [40-689]T 0.0 PTHR11733 Peptidase_M13 Peptidase_M13
PF01431 [485-686]T 1.3000049540726403E-107 PF01431 Peptidase_M13 Peptidase_M13
InterPro
IPR006025
Binding_site
Peptidase M, neutral zinc metallopeptidases, zinc-binding site
PS00142 [523-532]? 0.0 PS00142 ZINC_PROTEASE ZINC_PROTEASE
InterPro
IPR008753
Domain
Peptidase M13
PF05649 [53-433]T 0.0 PF05649 Peptidase_M13_N Peptidase_M13_N
noIPR
unintegrated
unintegrated
G3DSA:3.40.390.10 [351-689]T 1.9000069431513903E-106 G3DSA:3.40.390.10 G3DSA:3.40.390.10 G3DSA:3.40.390.10
PTHR11733:SF18 [40-689]T 0.0 PTHR11733:SF18 PTHR11733:SF18 PTHR11733:SF18
PS51257 [1-23]T 0.0 PS51257 PROKAR_LIPOPROTEIN PROKAR_LIPOPROTEIN
SSF55486 [30-689]T 0.0 SSF55486 SSF55486 SSF55486


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PR00786 (Neprilysin metalloprotease (M13) family signature) with a combined E-value of 1.3e-37.
    PR00786A    477-489
    PR00786B    495-507
    PR00786C    516-532
    PR00786D    587-598


 ProDom Summary:  Protein Domain Search
Residues 205-318 are 99% similar to a (PEPO) protein domain (PD217128 which is seen in O50642_PORGI.

Residues 1-43 are identical to a (PEPO) protein domain (PD142967 which is seen in O50642_PORGI.

Residues 44-204 are identical to a (ZINC METALLOPROTEASE PROTEIN HYDROLASE) protein domain (PD002031 which is seen in O50642_PORGI.

Residues 633-689 are 98% similar to a (METALLOPROTEASE ZINC ENDOPEPTIDASE O) protein domain (PD011850 which is seen in O50642_PORGI.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
Residues 53 to 433 (E-value = 2.6e-190) place PG0146 in the Peptidase_M13_N family which is described as Peptidase family M13 (PF05649)
Residues 485 to 686 (E-value = 1.1e-107) place PG0146 in the Peptidase_M13 family which is described as Peptidase family M13 (PF01431)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  
may be a lipoprotein  
  

Top PDB Hits:
-49% similar to PDB:1DMT STRUCTURE OF HUMAN NEUTRAL ENDOPEPTIDASE COMPLEXED WITH PHOSPHORAMIDON (E_value = 3.4E_89);
-49% similar to PDB:1R1H STRUCTURAL ANALYSIS OF NEPRILYSIN WITH VARIOUS SPECIFIC AND POTENT INHIBITORS (E_value = 3.4E_89);
-49% similar to PDB:1R1I STRUCTURAL ANALYSIS OF NEPRILYSIN WITH VARIOUS SPECIFIC AND POTENT INHIBITORS (E_value = 3.4E_89);
-49% similar to PDB:1R1J STRUCTURAL ANALYSIS OF NEPRILYSIN WITH VARIOUS SPECIFIC AND POTENT INHIBITORS (E_value = 3.4E_89);
-49% similar to PDB:1Y8J Crystal Structure of human NEP complexed with an imidazo[4,5-c]pyridine inhibitor (E_value = 3.4E_89);

Gene Protein Sequence:
MNKTIKFLCLAPAVIGALMLTGCNGNKGQTNDTDRKREPVPAIDLSAMDT
SVRPQDDFYRYCNGNWMKNNPLKPAYSRYGSFDILHDSTLERVHLIVDNL
AAGQHEVGTNEYRIATLYRQAMDSIKRNKDGAAPLKEDLQKIEAIADRAA
MVKYAAAKDNMGGSTFFGSYVYADAKNSEMNIFHITQTGLALDNRDYYLK
QDAKSQQIREAYVAYLNKIAKLAGYDDEAATRIAKNAMKMETELAQICYS
KEELRDTHRNYNKMAVKEFTNKYQGFDWTTYLADRQLTTLEEWDVEQLDF
FKKFDSWFAKADLNEMRDYLLAGTISGAASYLSDDFEQARFDFFGKTLSG
TTEMHPRWKRSVGMVSSFLGEALGEVYVKQYFPPEAKERMLKLVKNLQTA
LGERINMLTWMGDSTKMKAQEKLNSFIIKIGYPDKWKDYSKMEIKGDSYY
ADIKRASKWMHDDNMADLGKTVDRERWLMNPQDVNAYYNPTTNEICFPAA
ILQPPFFNMDADDAVNYGGIGVVIGHEMTHGFDDQGRNFDKDGNMINWWT
AEDAQKFETTARKLADQFSEIYVADGVRANGNMTLGENIADQGGLLISYL
AFRNAAKGEVMEEIDGFTPDQRFFIGYARLWGQNIRPEEVLRLTQIDVHS
LGELRVNQALRNIEAFYEAFNIQPTDKMYLEPEKRVVVW

Gene Nucleotide Sequence:  Sequence Viewer
ATGAACAAGACAATCAAGTTTCTTTGCCTTGCGCCGGCAGTAATCGGCGC
ATTGATGCTGACCGGCTGCAATGGCAATAAGGGTCAGACTAACGATACTG
ACAGGAAGCGCGAACCGGTACCGGCCATCGACCTCAGTGCCATGGATACA
TCCGTACGCCCGCAGGACGACTTTTATCGCTACTGCAACGGCAATTGGAT
GAAAAACAATCCTCTCAAACCTGCTTATAGTCGCTACGGCTCATTCGACA
TCCTGCACGACAGCACCCTCGAGCGTGTACACCTGATTGTGGACAACCTT
GCAGCAGGACAGCATGAAGTCGGCACTAATGAGTATCGTATAGCTACGCT
CTATCGTCAAGCTATGGATAGCATCAAACGGAATAAAGACGGAGCAGCCC
CTCTGAAGGAAGATCTTCAGAAGATCGAAGCCATTGCGGACAGAGCTGCT
ATGGTGAAGTATGCCGCTGCTAAGGACAATATGGGTGGAAGCACATTCTT
CGGATCGTACGTTTATGCCGACGCCAAGAATAGCGAGATGAACATCTTCC
ATATCACCCAGACCGGCTTGGCTCTTGACAATCGAGACTACTATCTCAAG
CAGGATGCCAAGTCTCAGCAAATACGCGAGGCGTATGTAGCTTATCTGAA
CAAAATTGCTAAGCTGGCCGGCTACGACGATGAAGCAGCTACCCGTATTG
CCAAGAACGCCATGAAGATGGAAACCGAACTGGCACAAATCTGCTACAGC
AAAGAAGAACTACGCGATACCCATCGTAACTACAATAAGATGGCAGTGAA
GGAGTTTACGAATAAGTACCAAGGCTTTGATTGGACTACTTACCTCGCCG
ATCGTCAGCTCACCACCCTCGAAGAGTGGGATGTGGAGCAACTCGATTTC
TTCAAGAAGTTCGACTCTTGGTTTGCCAAAGCCGATCTGAACGAAATGAG
GGACTATCTCCTCGCCGGCACGATCAGCGGTGCAGCAAGCTATCTGAGCG
ATGACTTCGAGCAAGCTCGTTTCGACTTTTTCGGTAAGACGCTGAGTGGA
ACCACCGAAATGCATCCTCGCTGGAAACGTTCCGTAGGTATGGTAAGCAG
CTTTCTGGGCGAAGCACTGGGCGAAGTTTATGTAAAGCAATACTTCCCAC
CCGAAGCAAAAGAGCGCATGCTCAAACTCGTCAAGAACCTGCAAACGGCC
CTCGGAGAGCGTATCAACATGCTTACATGGATGGGCGACTCCACGAAGAT
GAAAGCTCAGGAAAAACTCAATTCCTTCATCATCAAGATCGGTTATCCCG
ACAAGTGGAAAGACTATTCCAAGATGGAAATCAAGGGAGACAGCTATTAT
GCCGATATCAAACGCGCCAGCAAATGGATGCATGACGACAATATGGCTGA
CCTCGGCAAGACTGTAGATCGCGAGCGTTGGCTGATGAATCCTCAGGATG
TCAATGCCTACTACAATCCGACCACAAACGAAATTTGTTTTCCTGCAGCC
ATCCTCCAACCGCCTTTCTTCAACATGGATGCAGACGATGCTGTCAATTA
TGGCGGTATCGGCGTAGTGATCGGACACGAGATGACGCATGGATTCGACG
ATCAAGGCCGCAACTTCGACAAAGACGGCAATATGATCAACTGGTGGACT
GCTGAAGATGCTCAGAAGTTCGAGACCACAGCCCGAAAACTGGCCGATCA
GTTCAGCGAGATCTACGTAGCCGATGGTGTTCGTGCCAATGGTAATATGA
CACTTGGCGAGAACATCGCAGATCAGGGCGGTCTCCTCATCTCTTATCTG
GCTTTCCGCAATGCTGCCAAGGGTGAGGTAATGGAAGAAATCGACGGATT
CACTCCCGACCAGCGTTTCTTTATCGGTTATGCCCGTCTATGGGGACAAA
ATATTCGTCCGGAAGAGGTTCTCCGTCTCACACAGATCGATGTGCACAGT
TTGGGTGAGCTGCGCGTCAATCAAGCACTGCGCAATATCGAAGCTTTCTA
CGAAGCTTTCAATATACAGCCTACTGATAAGATGTATTTGGAACCCGAAA
AGCGTGTAGTCGTTTGG

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