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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0145 IGR0147 IGR0146 IGR0152 IGR0151 IGR0149 IGR0150 IGR0148 PG0181 PG0181.1 PG0177 PG0176 PG0179 uppS, - PG0174 ISPg3, - PG0178 PG0175 PG0180 PG0181 PG0181.1 PG0177 PG0176 PG0179 uppS, - PG0174 ISPg3, - PG0178 PG0175 PG0180 Type: IS element, Name: ISPg3 - 5 Type: tandem, Name: tan35 - 243 Type: tandem, Name: tan36 - 244 PG0181.1 PG0181 PG0176 PG0179 uppS, - PG0174 ISPg3, - PG0178 PG0175 PG0180 PG0177


LANL Gene ID: PG0178

GenBank Locus Tag: PG0194

DNA Molecule Name:
1  

Genomic Island ID:
4

GenBank ID:
34540050

 Gene Name:
ISPg3  

 Definition:
ISPg3 (IS195) transposase

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
231068

Gene Stop:
231967

Gene Length:
900

Molecular Weight*:
35065

pI*:
10.30

Net Charge*:
20.02

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


 Pathway: pathway table

 Primary Evidence:
Lewis JP, Macrina FL, IS195, an insertion sequence-like element associated with protease genes in Porphyromonas gingivalis. Infect Immun 1998 Jul;66(7):3035-42, PubMed: 9632563.

 Secondary Evidence:
Kehrenberg C, Werckenthin C, Schwarz S, Tn5706, a transposon-like element from Pasteurella multocida mediating tetracycline resistance. Antimicrob Agents Chemother 1998 Aug;42(8):2116-8, PubMed: 9687418.

 Comment:
TIGR ID: PG0194

 Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0178 is equivalent to the previously sequenced gb|AAC26596.1, a predicted IS195 transposase. Residues 75-286 are 52% similar to a transposase sequence from Pasteurella multocida (Y15510).

Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540050|ref|NP_904529.1|  ISPg3, transposase [Porphyromo...   609   e-173
 gi|34540105|ref|NP_904584.1|  ISPg3, transposase [Porphyromo...   607   e-172
 gi|34540580|ref|NP_905059.1|  ISPg3, transposase [Porphyromo...   603   e-171
 gi|3323385|gb|AAC26596.1|  putative protease [Porphyromonas ...   601   e-170
 gi|61652425|gb|AAX47722.1|  transposase [Porphyromonas gingi...   599   e-170
 gi|34540773|ref|NP_905252.1|  ISPg3, transposase [Porphyromo...   595   e-168
 gi|34540967|ref|NP_905446.1|  ISPg3, transposase [Porphyromo...   587   e-166
 gi|150009840|ref|YP_001304583.1|  putative transposase [Para...   306   8e-82
 gi|150002628|ref|YP_001297372.1|  putative transposase [Bact...   295   3e-78
 gi|89142213|ref|YP_512234.1|  transposase [Pasteurella treha...   278   3e-73


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [113-279]T 9.59999914716153E-30 PF01609 Transposase_11 Transposase_11
noIPR
unintegrated
unintegrated
SSF50249 [235-300]T 0.0047 SSF50249 Nucleic_acid_OB Nucleic_acid_OB


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 212-286 are 97% similar to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190030 which is seen in O87010_PORGI.

Residues 135-208 are identical to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in O87010_PORGI.

Residues 75-133 are identical to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190029 which is seen in O87010_PORGI.

Residues 1-74 are 97% similar to a (PUTATIVE PROTEASE PROTEASE) protein domain (PD203593 which is seen in O87010_PORGI.

Residues 116-280 are 29% similar to a (HYPOTHETICAL 20.1 KD PROTEIN) protein domain (PD207068 which is seen in Q06444_BACST.


Paralogs:  Local Blast Search
PG0178 is identical to PG0919, PG0236, PG0178 and PG1115, predicted ISPg3 transposases. PG0178 is similar to fragments of PG1663, PG1328, PG1660, PG0273.

Pfam Summary:  Pfam Search
Residues 113 to 279 (E-value = 3.3e-33) place PG0178 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)

 Structural Feature(s):
Feature Type  Start  Stop
coil-coil  
220  
251

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MKTNIVDVFCIIDDFSKLFDEAIKKKTLEEADKKRRNRKFKMSDSEVMTI
LILFHLSRYRDLKAFYLQYITYSCRSEFPHLVSYNRFVELQSRVGFKLIA
FLNMCCLGQCTGISFIDSTPLKACHIKRAHGHRTMRGWAQKGKSTMGWFY
GFKLHIVINDRGEIINYQITPGNCDDREPLKDGTFTKNLFGKLIADRGYI
SQNLFDRLFVDDIHMITKIKKNMKNSLMHLYDKVLLRKRALFETVNDMLK
NVCQIEHTRHRSANNFVTNLISGIIAYNILPKKPELNIEIIRNPNFPISA


Gene Nucleotide Sequence:  Sequence Viewer
ATGAAGACAAATATAGTTGATGTTTTTTGCATCATAGATGATTTCTCCAA
GCTTTTTGATGAAGCAATCAAGAAAAAGACCCTCGAAGAGGCAGACAAAA
AACGCAGGAATAGAAAGTTTAAGATGTCGGACAGTGAGGTCATGACCATC
CTGATCCTGTTTCATCTGTCAAGATACCGAGATTTGAAAGCTTTTTATCT
TCAATACATCACCTATTCTTGTCGATCCGAGTTCCCACATCTTGTCTCTT
ATAATCGCTTTGTGGAGCTGCAAAGCAGGGTGGGTTTCAAGCTGATAGCA
TTTCTCAATATGTGTTGTTTGGGTCAATGTACAGGCATCTCTTTCATCGA
TTCCACCCCACTGAAGGCTTGTCATATCAAACGAGCTCATGGGCATAGGA
CAATGAGGGGATGGGCTCAAAAAGGCAAAAGCACCATGGGTTGGTTTTAT
GGATTCAAACTGCATATTGTTATCAACGACAGGGGTGAAATCATCAACTA
TCAAATCACACCGGGCAATTGTGATGACAGAGAACCTCTGAAAGACGGAA
CATTCACCAAGAATCTTTTTGGCAAACTCATTGCCGATAGAGGCTACATT
TCCCAAAACCTTTTTGACCGGCTCTTTGTCGATGACATCCACATGATAAC
CAAAATCAAAAAGAACATGAAGAACTCCCTGATGCATCTATATGACAAAG
TTTTATTGAGAAAGAGAGCCCTGTTCGAAACGGTCAATGATATGCTCAAA
AATGTCTGTCAGATAGAGCACACGAGACATCGCAGTGCCAACAATTTTGT
CACCAACCTGATCTCCGGTATCATCGCTTACAACATCCTGCCTAAAAAGC
CTGAACTCAATATTGAAATCATCAGAAACCCTAACTTTCCTATTTCCGCT

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