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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0208 IGR0211 IGR0210 IGR0209.1 IGR0212 IGR0213 IGR0209 trxA, - PG0246 PG0245.1 PG0248 PG0252 PG0251 PG0250 PG0245 ISPg2, - PG0249 tlyC, - PG0244 trxA, - PG0246 PG0245.1 PG0248 PG0252 PG0251 PG0250 PG0245 ISPg2, - PG0249 tlyC, - PG0244 Type: IS element, Name: ISPg2 - 4 trxA, - PG0246 PG0245.1 PG0248 PG0252 PG0251 PG0250 PG0245 ISPg2, - PG0249 tlyC, - PG0244


LANL Gene ID: PG0249

GenBank Locus Tag: PG0277

DNA Molecule Name:
1  

Genomic Island ID:
21

GenBank ID:
34540119

 Gene Name:
ISPg2  

 Definition:
ISPg2 (PGIS2) transposase

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
308996

Gene Stop:
310123

Gene Length:
1128

Molecular Weight*:
43313

pI*:
7.40

Net Charge*:
2.35

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


 Pathway: pathway table

 Primary Evidence:
Wang,C.Y., Bond,V.C. and Genco,C.A.
Identification of a second endogenous Porphyromonas gingivalis
insertion element. J. Bacteriol. 179 (11), 3808-3812 (1997)

 Comment:
Note BLAST similarities to E.coli and V.cholerae proteins.

Member of IS5 family of elements.

TIGR ID: PG0277

 Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0249 is equivalent to the previously sequenced gb|AAC45368.1 in GenbBANK. Residues 4-364 are 32% similar to an uncharacterized sequence from E.coli 0157:H7 (AE005200). Residues 11-358 are 31% similar to the RfbQRSO22-3 sequence of V.cholerae (AF004383).

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540119|ref|NP_904598.1|  ISPg2, transposase [Porphyromo...   717   0.0  
 gi|91214690|ref|ZP_01251663.1|  putative H repeat-associated...   222   3e-56
 gi|91215887|ref|ZP_01252856.1|  putative H repeat-associated...   221   5e-56
 gi|56475478|ref|YP_157067.1|  transposase (IS4) [Azoarcus sp...   187   1e-45
 gi|56475480|ref|YP_157069.1|  transposase (IS4) [Azoarcus sp...   187   1e-45
 gi|87307470|ref|ZP_01089614.1|  hypothetical protein DSM3645...   180   1e-43
 gi|87310239|ref|ZP_01092370.1|  putative H repeat-associated...   180   1e-43
 gi|87308374|ref|ZP_01090515.1|  hypothetical protein DSM3645...   179   2e-43
 gi|34540798|ref|NP_905277.1|  ISPg6, transposase [Porphyromo...   178   5e-43
 gi|119898341|ref|YP_933554.1|  putative H repeat-associated ...   176   2e-42


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [110-344]T 5.69999708784002E-36 PF01609 Transposase_11 Transposase_11


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01811 (Transposase) with a combined E-value of 2e-24.
    PF01811A    0-35
    PF01811B    36-52
    PF01811E    134-181
    PF01811F    183-207


 ProDom Summary:  Protein Domain Search
Residues 132-264 are identical to a (PROTEIN TRANSPOSASE REGION PUTATIVE) protein domain (PD004339 which is seen in P95492_PORGI.

Residues 332-376 are identical to a (PUTATIVE TRANSPOSASE) protein domain (PD054580 which is seen in P95492_PORGI.

Residues 279-331 are identical to a (PROTEIN TRANSPOSASE PUTATIVE REGION) protein domain (PD004012 which is seen in P95492_PORGI.

Residues 1-131 are 83% similar to a (PUTATIVE TRANSPOSASE) protein domain (PD103322 which is seen in P95492_PORGI.


Paralogs:  Local Blast Search
PG0249 is identical to PG1522, PG1185, PG0775 and PG1905, all predicted ISPg2 elements. PG0249 is similar to PG0943 and weakly similar to PG1058, PG0889, PG1795 and PG1855.

Pfam Summary:  Pfam Search
Residues 110 to 344 (E-value = 5.8e-39) place PG0249 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
22  
38
coil-coil  
43  
70
non-globular  
331  
376

Top PDB Hits:
-47% similar to PDB:2IPX Human Fibrillarin (E_value = );

Gene Protein Sequence:
MYHSLFESLCMVPDPRIERKKIYPLDFLLLIVFLSTLSGNTSWYEIEDYA
EEYEEELKSLYEMLTGHQLMHTMPSHDTLNRSISLLDVEAFEGAYKRWIE
GFISATSGKHICIDGKTMRGVKKLSFDTQSHVVSAFSPQDMCSLAQLYID
RKTNEIPAIHQLLDLLDLNGAVVSIDAIGTQTAIAEQIIDKGGNYVLCVK
ANQSLSLQEIEAYFCPLFQKHILLDEQTELSHGRIETRRYESILNPLEIE
ANEVLTRWKGLRSIHKVVRKRRDKKSDKTSEEVAYYISSLTDVSSLKQAI
RGHWAIENKLHHCLDVYFGHDASHKRTRNVAQIMDIIQKINLLIIERLKT
NMKSSIPRIQKKLARMKPQQLITIQF

Gene Nucleotide Sequence:  Sequence Viewer
ATGTATCATTCTCTATTTGAAAGCCTCTGCATGGTTCCGGACCCACGGAT
AGAGCGTAAAAAAATTTATCCTCTTGATTTTTTACTGCTGATCGTTTTCC
TCTCTACACTCTCAGGCAATACCTCTTGGTATGAGATTGAAGATTATGCC
GAGGAATACGAAGAAGAATTGAAAAGTCTATACGAAATGCTTACCGGCCA
TCAGCTTATGCATACCATGCCTTCTCATGATACTCTCAACAGGAGCATCA
GTCTGTTGGATGTAGAAGCTTTTGAGGGGGCTTATAAGCGATGGATTGAA
GGCTTTATCTCGGCCACTTCGGGTAAACATATTTGCATTGATGGCAAGAC
GATGCGGGGAGTGAAGAAACTCTCTTTCGATACACAATCCCATGTCGTCT
CTGCCTTTTCACCACAAGATATGTGCAGTCTTGCCCAACTCTACATCGAC
CGAAAAACAAACGAAATACCGGCTATACATCAACTTCTTGATTTGCTTGA
CTTGAACGGGGCTGTTGTCTCCATTGATGCCATAGGTACACAAACTGCCA
TTGCCGAACAAATTATCGATAAGGGCGGAAACTATGTATTGTGTGTTAAA
GCGAATCAAAGTTTGAGTCTGCAAGAGATTGAAGCCTATTTCTGCCCTCT
TTTTCAGAAACATATCCTCCTTGACGAACAGACGGAACTATCTCACGGAC
GCATAGAAACACGTCGCTATGAAAGTATTCTCAATCCCTTGGAGATAGAA
GCCAACGAGGTATTAACTCGCTGGAAAGGCTTGAGGTCGATACACAAAGT
TGTACGCAAACGAAGGGATAAAAAGAGTGACAAAACGAGTGAAGAAGTGG
CCTACTACATTTCGTCATTAACAGATGTTTCTTCATTGAAACAAGCTATT
CGTGGGCATTGGGCGATAGAGAATAAGTTACACCACTGTTTGGATGTCTA
TTTCGGACACGATGCCTCGCACAAGAGAACGAGGAATGTGGCGCAGATTA
TGGATATCATTCAAAAGATTAATTTACTCATTATAGAGAGACTAAAAACG
AATATGAAGTCCTCAATCCCTCGCATCCAAAAGAAACTGGCTCGAATGAA
GCCACAACAACTAATCACAATACAATTT

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