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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0354 IGR0350 IGR0351 IGR0353 IGR0348 IGR0352 IGR0349 ISPg5, - PG0419 ISPg5, - PG0418 folC, - PG0423 ISPgX, - PG0421 PG0422 ISPg1, - PG0420 PG0416 ISPg5, - PG0419 ISPg5, - PG0418 folC, - PG0423 ISPgX, - PG0421 PG0422 ISPg1, - PG0420 PG0416 Type: IS element, Name: ISPg1 - 2 Type: IS element, Name: ISPg5 - 8 Type: IS element, Name: ISPg5 - 8 ISPg5, - PG0419 ISPg5, - PG0418 folC, - PG0423 ISPgX, - PG0421 PG0422 ISPg1, - PG0420 PG0416 PG0417 PG0417


LANL Gene ID: PG0420

GenBank Locus Tag: PG0460

DNA Molecule Name:
1  

Genomic Island ID:
22

GenBank ID:
34540279

 Gene Name:
ISPg1  

 Definition:
ISPg1 transposase (IS1106-related transposase)

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
502078

Gene Stop:
503160

Gene Length:
1083

Molecular Weight*:
41575

pI*:
10.20

Net Charge*:
14.72

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


 Pathway: pathway table

 Primary Evidence:
Hanley,S.A., Aduse-Opoku,J. and Curtis,M.A.
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis
W50 has arisen via horizontal gene transfer
Infect. Immun. 67 (3), 1157-1171 (1999)

Maley,J. and Roberts,I.S.
Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements
Microbiol. Lett. 123 (1-2), 219-224 (1994)

Shibata,Y., Hayakawa,M., Takiguchi,H., Shiroza,T. and Abiko,Y.
Determination and characterization of the hemagglutinin-associated
short motifs found in Porphyromonas gingivalis multiple gene
products. J. Biol. Chem. 274 (8), 5012-5020 (1999)

 Secondary Evidence:
Knight,A.I., Ni,H., Cartwright,K.A. and McFadden,J.J.
Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidis.
Mol. Microbiol. 6 (11), 1565-1573 (1992)

 Comment:
Member of the IS5 family of elements. A full-length ISPg1 element.

TIGR ID: PG0460

 Proteomic Data: Proteomic Data Search
TIGR AnnotationISPg1, transposase, GroupL (other categories)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG0460-0.940.018-1.254.2e-05-1.160.00053-1.254.2e-05
LANLPG04200.520.420.450.36
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0420 is equivalent to the previously sequenced AB015879 in GenBANK. See also AJ130872. Residues 31-350 are 34% similar to the IS1106 transposase of N. meningitidis (AE002488).

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540279|ref|NP_904758.1|  ISPg1, transposase [Porphyromo...   715   0.0  
 gi|4001716|dbj|BAA35090.1|  ORF7 [Porphyromonas gingivalis]       704   0.0  
 gi|34540041|ref|NP_904520.1|  ISPg1, transposase [Porphyromo...   680   0.0  
 gi|3901097|emb|CAA10225.1|  transposase [Porphyromonas gingi...   675   0.0  
 gi|1361314|pir||S57895  hypothetical protein 1 - Porphyromon...   583   e-165
 gi|3869078|dbj|BAA34342.1|  Insertion sequence IS1126-like g...   307   6e-82
 gi|91218509|ref|ZP_01255448.1|  ISPg1, transposase [Psychrof...   306   2e-81
 gi|34541013|ref|NP_905492.1|  ISPg1, transposase, internal d...   302   2e-80
 gi|91216745|ref|ZP_01253710.1|  ISPg1, transposase [Psychrof...   246   2e-63
 gi|15677391|ref|NP_274546.1|  IS1106 transposase [Neisseria ...   187   9e-46


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [147-348]T 1.2000011745813999E-32 PF01609 Transposase_11 Transposase_11


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 157-302 are 97% similar to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in Q9ZAD0_PORGI.

Residues 12-109 are 98% similar to a (TRANSPOSASE PROTEIN INSERTION ELEMENT) protein domain (PD007264 which is seen in Q9ZAD0_PORGI.

Residues 303-361 are 96% similar to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187845 which is seen in Q9ZAD0_PORGI.

Residues 110-156 are identical to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187846 which is seen in Q9ZA61_PORGI.


Paralogs:  Local Blast Search
PG0420 is virtually identical to PG1267, PG0169, PG1050, PG1062, PG0740, PG0918, PG1422, PG1662, PG0499, and PG1099.

Partial similarities exist to PG0732, PG0842, PG1607, PG0731, PG0884, PG1184, PG1087, PG0763, PG1755, PG0686, PG0756, PG1798, PG1230, PG1919, PG0044, PG0689, PG1215, PG1665, PG0839, PG1895, PG0883, PG11231, PG0730, PG1159 and PG1608.

Pfam Summary:  Pfam Search
Residues 147 to 348 (E-value = 9.4e-36) place PG0420 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
58  
74
non-globular  
168  
218

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MAYQSKNTDEHVTFADALLSKRYRKAQNDFLNQVERLIDWRPIRTLINKK
YTKRQNAIGAPAYDVILLFKMLLLETWYNLSDCALEERINDSITFSRFLG
LKMEEVSPDHSTISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLV
DASLVETPHKPNGTITIEVADDREDNRSEAEKEAEEDYQKQVVRRRKGTD
EEARWVYKQKRYHYGYKKHCLTNVQGIVQKVITTAANRSDTKEFIPLLQG
ANIPQGTAVLADKGYACGENRSYLQTHHLQDGIMHKAQRNRALTEEEKQG
NKAISPIRSTIERTFGSIRRWFHGGRCRYRGLAKTHTQNILESIAFNLYR
TPGIIMSSSLG

Gene Nucleotide Sequence:  Sequence Viewer
ATGGCATACCAATCCAAGAATACCGATGAGCATGTAACATTTGCAGACGC
ACTCCTTTCAAAGCGTTATCGCAAAGCACAAAACGACTTCCTCAATCAGG
TTGAGAGGCTTATCGATTGGCGTCCGATCAGGACGCTGATCAACAAGAAA
TACACGAAGCGACAAAATGCCATCGGCGCCCCGGCTTATGACGTGATTCT
CTTATTCAAGATGTTGCTTTTGGAGACATGGTACAACCTCAGTGATTGTG
CTTTGGAGGAGCGCATCAATGATTCAATCACCTTTTCCCGATTCTTGGGA
CTGAAGATGGAAGAGGTATCTCCCGACCACAGCACCATCAGTCGATTTCG
TTCGGCACTGACAGAGTTGGGGCTCATGGACAAACTATTGGCGCAGTTTA
ACAAACAACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTC
GATGCAAGCCTTGTGGAGACGCCACATAAACCCAACGGAACCATTACGAT
TGAAGTCGCAGACGACAGAGAAGACAATCGGAGCGAGGCGGAAAAAGAGG
CAGAGGAGGATTATCAAAAACAGGTTGTCCGCCGGCGTAAAGGGACGGAT
GAAGAAGCCCGTTGGGTGTACAAACAAAAGCGTTATCACTACGGATACAA
AAAGCATTGTCTGACCAATGTTCAAGGCATTGTTCAAAAGGTGATAACGA
CTGCAGCGAACCGCAGTGACACGAAGGAGTTTATTCCGCTATTGCAGGGT
GCAAACATACCTCAAGGCACAGCCGTCTTGGCGGACAAAGGATATGCTTG
CGGGGAAAATCGTTCCTACCTGCAAACCCATCACCTTCAAGACGGCATCA
TGCACAAGGCACAACGCAACAGGGCATTGACCGAGGAAGAGAAGCAAGGA
AACAAAGCAATCAGTCCGATACGGAGCACCATCGAACGCACCTTTGGCAG
TATTCGCCGGTGGTTTCATGGCGGACGATGTCGATACCGGGGACTTGCCA
AGACCCATACTCAAAACATTCTTGAAAGCATCGCCTTTAATTTATACAGA
ACCCCGGGGATAATTATGTCCTCATCTCTAGGA

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