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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0368 IGR0373 IGR0366 IGR0370 IGR0374 IGR0367 IGR0369 IGR0371 IGR0372 PG0443 PG0440 dat1, - PG0444 PG0441 PG0442 ISPg4, - PG0445 kbl, - PG0439 ruvB, - PG0446 PG0448 PG0447 PG0443 PG0440 dat1, - PG0444 PG0441 PG0442 ISPg4, - PG0445 kbl, - PG0439 ruvB, - PG0446 PG0448 PG0447 Type: IS element, Name: ISPg4 - 7 PG0443 PG0440 dat1, - PG0444 PG0441 PG0442 ISPg4, - PG0445 kbl, - PG0439 ruvB, - PG0446 PG0448 PG0447


LANL Gene ID: PG0445

GenBank Locus Tag: PG0487

DNA Molecule Name:
1  

Genomic Island ID:
22

GenBank ID:
34540303

 Gene Name:
ISPg4  

 Definition:
ISPg4 transposase

 Cellular Location:
Inner membrane, Cytoplasm [Evidence]

 Gene Start:
527524

Gene Stop:
526370

Gene Length:
1155

Molecular Weight*:
44087

pI*:
10.20

Net Charge*:
16.13

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


 Pathway: pathway table

 Primary Evidence:
Sawada,K., Kokeguchi,S., Hongyo,H., Sawada,S., Miyamoto,M.,
Maeda,H., Nishimura,F., Takashiba,S. and Murayama,Y.
Identification by subtractive hybridization of a novel insertion
sequence specific for virulent strains of porphyromonas gingivalis. Infect. Immun. 67 (11), 5621-5625 (1999)

 Secondary Evidence:
Nagai,T., Phan Tran,L.S., Inatsu,Y. and Itoh,Y.
A new IS4 family insertion sequence, IS4Bsu1, responsible for
genetic instability of poly-gamma-glutamic acid production in
bacillus subtilis. J. Bacteriol. 182 (9), 2387-2392 (2000)

 Comment:
Member of the IS5 family of IS elements.

TIGR ID: PG0487

 Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0445 is equivalent to the previously sequenced AF148127 in GenBANK. See also AB009361, AB003149. Residues 20-337 are 27% similar to a transposase from B.subtilis (AB031551).

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540035|ref|NP_904514.1|  ISPg4, transposase [Porphyromo...   753   0.0  
 gi|34539897|ref|NP_904376.1|  ISPg4 transposase [Porphyromon...   753   0.0  
 gi|34540966|ref|NP_905445.1|  ISPg4, transposase [Porphyromo...   752   0.0  
 gi|34539923|ref|NP_904402.1|  ISPg4, transposase [Porphyromo...   751   0.0  
 gi|5763509|dbj|BAA83477.1|  transposase [Porphyromonas gingi...   748   0.0  
 gi|5763505|dbj|BAA83475.1|  transposase [Porphyromonas gingi...   497   e-139
 gi|150384831|ref|ZP_01923500.1|  transposase, IS4 family pro...   296   1e-78
 gi|150385323|ref|ZP_01923963.1|  transposase, IS4 family pro...   295   3e-78
 gi|5763507|dbj|BAA83476.1|  transposase [Porphyromonas gingi...   243   2e-62
 gi|67941161|ref|ZP_00533393.1|  Transposase, IS4 [Chlorobium...   226   2e-57


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [124-335]T 5.0000090991531485E-40 PF01609 Transposase_11 Transposase_11
InterPro
IPR012337
Domain
Polynucleotidyl transferase, Ribonuclease H fold
SSF53098 [185-350]T 2.3000000000000003E-22 SSF53098 RNaseH_fold RNaseH_fold


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01609 (Transposase (IS4 family)) with a combined E-value of 1.4e-08.
    PF01609B    288-309


 ProDom Summary:  Protein Domain Search
Residues 271-336 are 45% similar to a (TRANSPOSASE PUTATIVE PLASMID ELEMENT) protein domain (PD003301 which is seen in Y4ZB_RHISN.


Paralogs:  Local Blast Search
PG0445 is paralogously related to PG1461, PG1114, PG1920, PG0043, PG1447, PG0868, PG00162, PG0205, and PG0016, all predicted ISPg4-related transposases.

Pfam Summary:  Pfam Search
Residues 124 to 335 (E-value = 6.9e-43) place PG0445 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)

 Structural Feature(s):
Feature Type  Start  Stop
uncleavable signal  
1  
51
transmembrane  
321  
337

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MSTNISLFAQVIRLLPRPLIKKLSTEFQVDKHSKHFTSWQHLVSMIFSQF
SSCISLREISNGLRSATGNLNHLGICTAPSKSNLSYQNEHRTSDFFRACY
YALLDYFGQQGMGQGRKFRFKQPVKLLDSTTITLCLALYDWAKYTHTKGA
VKLHTLLDFKTLLPEYVHISDGKGHDGKMANSIPIPAGSIVVADRGYADT
ALLNSWDSTQVSFVVRHPRSLKYEVIQELELPEHGHQQILVDQRVRLTGV
QTQGKYTKPLRHIALYNEQHGDVVELLTNIDTLAASSIALLYRSRWLIEI
FFRNLKQRLSMKAFLGTTRNAVEVQIWTALITMLLMVYLKSIAKYRWCLS
NLVSSLRINTFTKMDLMQWLNEPFTPPPEPENQLF

Gene Nucleotide Sequence:  Sequence Viewer
ATGAGTACAAATATAAGCCTATTCGCCCAAGTTATCCGCCTGTTGCCTCG
TCCATTGATTAAGAAGCTCTCTACAGAGTTTCAGGTAGACAAGCACAGCA
AGCATTTCACCTCATGGCAGCACTTAGTGAGCATGATTTTCAGCCAATTC
TCAAGCTGTATCTCCTTACGCGAGATTAGTAATGGCTTACGCTCAGCCAC
AGGTAACCTCAACCACTTAGGCATATGCACAGCACCCTCTAAGTCCAATT
TATCTTATCAGAACGAGCACCGTACCTCAGACTTCTTTCGAGCGTGCTAT
TATGCCTTGCTAGATTATTTCGGACAGCAGGGTATGGGGCAAGGGCGTAA
GTTTCGTTTTAAGCAACCCGTCAAGCTTCTAGACTCTACGACCATTACCC
TTTGCCTTGCCCTATACGATTGGGCTAAATACACACATACGAAGGGAGCT
GTTAAGCTTCATACACTCTTAGACTTCAAGACTCTGTTGCCTGAATATGT
GCATATTAGTGATGGTAAAGGACATGATGGCAAGATGGCCAATTCTATTC
CTATCCCAGCAGGAAGTATCGTCGTGGCTGACCGTGGCTATGCTGATACC
GCCTTGCTCAATAGTTGGGACAGCACCCAAGTGAGTTTTGTAGTACGCCA
CCCAAGGTCCTTAAAATATGAGGTTATTCAAGAGTTGGAACTCCCAGAAC
ATGGGCATCAGCAAATACTCGTAGATCAGAGAGTACGGCTCACAGGCGTT
CAGACGCAAGGCAAGTACACTAAGCCATTGAGGCATATTGCCCTCTACAA
CGAGCAACATGGCGATGTCGTTGAGTTATTGACCAACATAGACACCCTTG
CAGCAAGTAGTATAGCCTTGCTGTATCGTTCAAGATGGCTTATCGAGATT
TTCTTTCGGAATTTGAAGCAACGCCTCAGCATGAAGGCCTTTCTTGGAAC
AACGCGCAATGCCGTAGAGGTGCAAATATGGACGGCTCTTATTACAATGC
TTTTGATGGTCTATCTCAAGAGTATCGCCAAATATCGGTGGTGCTTGTCT
AATCTTGTCTCTTCGCTAAGAATCAACACATTTACCAAGATGGATCTGAT
GCAATGGCTTAATGAGCCGTTTACGCCTCCGCCAGAGCCCGAAAATCAGC
TCTTT

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