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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0383 IGR0384 IGR0385 IGR0389 IGR0386 IGR0382 IGR0388 IGR0387 IGR0381 PG0461.1 PG0455 smpB, - PG0458 luxS, - PG0454 lipA, - PG0460 PG0457 tgt, - PG0456 dppIV, - PG0459 rgpB,prtRII,rgp-2,prpR2, - PG0461 PG0461.1 PG0455 smpB, - PG0458 luxS, - PG0454 lipA, - PG0460 PG0457 tgt, - PG0456 dppIV, - PG0459 rgpB,prtRII,rgp-2,prpR2, - PG0461 PG0461.1 PG0455 smpB, - PG0458 luxS, - PG0454 lipA, - PG0460 PG0457 tgt, - PG0456 dppIV, - PG0459 rgpB,prtRII,rgp-2,prpR2, - PG0461


LANL Gene ID: PG0459

GenBank Locus Tag: PG0503

DNA Molecule Name:
1  

GenBank ID:
34540319

Gene Name:
dppIV  

Definition:
dipeptidyl peptidase IV (DPP IV) N-terminal region

Cellular Location:
Outer membrane, Cytoplasm, Extracellular [Evidence]

Gene Start:
542101

Gene Stop:
544269

Gene Length:
2169

Molecular Weight*:
81934

pI*:
7.40

Net Charge*:
2.19

EC:
3.4.14.11  3.4.14.5  

Functional Class:
Protein fate; Degradation of proteins, peptides and glycopeptides  

Gene Ontology:
Biological process
  GO:0006508    proteolysis

Cellular component
  GO:0016020    membrane

Molecular function
  GO:0004274    dipeptidyl-peptidase IV activity
  GO:0008236    serine-type peptidase activity


Pathway: pathway table

Primary Evidence:
Kumagai,Y., Konishi,K., Gomi,T., Yagishita,H., Yajima,A. and
Yoshikawa,M. 2000. Enzymatic properties of dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis and its participation in virulence. Infect. Immun. 68 (2): 716-724. PubMed: 10639438.

Kiyama,M., Hayakawa,M., Shiroza,T., Nakamura,S., Takeuchi,A., Masamoto,Y. and Abiko,Y. 1998. Sequence analysis of the Porphyromonas gingivalis dipeptidyl peptidase IV gene. Biochim. Biophys. Acta 1396 (1): 39-46. PubMed: 9524216.

Banbula A, Bugno M, Goldstein J, Yen J, Nelson D, Travis J, Potempa J. Emerging family of proline-specific peptidases of Porphyromonas gingivalis: purification and characterization
of serine dipeptidyl peptidase, a structural and functional homologue of mammalian prolyl dipeptidyl peptidase IV.

Nemoto E, Sugawara S, Takada H, Shoji S, Horiuch H.
Increase of CD26/dipeptidyl peptidase IV expression on human gingival
fibroblasts upon stimulation with cytokines and bacterial components.
Infect Immun. 1999 Dec;67(12):6225-33.
PMID: 10569731

Comment:
TIGR ID: PG0503

Proteomic Data: Proteomic Data Search
TIGR Annotationdipeptidyl aminopeptidase IV, GroupD (cellular processes) ,GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG0503-1.152.3e-08-0.930.00044-1.174.5e-13-0.930.00044
LANLPG04590.450.520.440.33
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Shares very strong similarity with a large number of bacterial dipeptidyl peptidase IV genes:
100% identical to dbj|BAA28265.1| (AB008194), a DPP IV previously reported in Porphyromonas gingivalis.
98% identical to gb|AAC46184.1| (AF026511, a) dipeptidyl peptidase IV previously reported in Porphyromonas gingivalis.

This sequence is similar to BT4193.


 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540319|ref|NP_904798.1|  dipeptidyl aminopeptidase IV [...  1475   0.0  
 gi|2570829|gb|AAC46184.1|  dipeptidyl peptidase IV [Porphyro...  1455   0.0  
 gi|150008803|ref|YP_001303546.1|  dipeptidyl aminopeptidase ...   942   0.0  
 gi|154494422|ref|ZP_02033742.1|  hypothetical protein PARMER...   929   0.0  
 gi|60680449|ref|YP_210593.1|  putative dipeptidyl peptidase ...   745   0.0  
 gi|53712269|ref|YP_098261.1|  dipeptidyl peptidase IV [Bacte...   744   0.0  
 gi|156859361|gb|EDO52792.1|  hypothetical protein BACUNI_035...   713   0.0  
 gi|29349601|ref|NP_813104.1|  dipeptidyl peptidase IV [Bacte...   709   0.0  
 gi|156107443|gb|EDO09188.1|  hypothetical protein BACOVA_050...   704   0.0  
 gi|153807515|ref|ZP_01960183.1|  hypothetical protein BACCAC...   693   0.0


InterPro Summary:  InterProScan

InterPro
IPR001375
Domain
Peptidase S9, prolyl oligopeptidase active site region
PF00326 [523-723]T 7.799983041256318E-59 PF00326 Peptidase_S9 Peptidase_S9
InterPro
IPR002469
Domain
Peptidase S9B, dipeptidylpeptidase IV N-terminal
PF00930 [98-441]T 0.0 PF00930 DPPIV_N DPPIV_N
noIPR
unintegrated
unintegrated
G3DSA:2.140.10.30 [22-471]T 2.3999979815735102E-108 G3DSA:2.140.10.30 G3DSA:2.140.10.30 G3DSA:2.140.10.30
G3DSA:3.40.50.1820 [473-723]T 2.0000139277203602E-81 G3DSA:3.40.50.1820 G3DSA:3.40.50.1820 G3DSA:3.40.50.1820
PTHR11731 [77-723]T 0.0 PTHR11731 PTHR11731 PTHR11731
PTHR11731:SF16 [77-723]T 0.0 PTHR11731:SF16 PTHR11731:SF16 PTHR11731:SF16
SSF53474 [473-723]T 1.6E-70 SSF53474 SSF53474 SSF53474
SSF82171 [22-471]T 1.4E-97 SSF82171 SSF82171 SSF82171


COGS Summary:  COGS Search
BeTs to 4 clades of COG1506
COG name: Dipeptidyl aminopeptidases/acylaminoacyl-peptidases
Functional Class: E
The phylogenetic pattern of COG1506 is ---KY--c-b-----------
Number of proteins in this genome belonging to this COG is 5
Blocks Summary:  Blocks Search
***** PF00930 (Dipeptidyl peptidase IV (DPP IV) N-terminal region) with a combined E-value of 2.6e-102.
    PF00930A    95-116
    PF00930B    174-224
    PF00930C    235-261
    PF00930D    276-308
    PF00930E    367-377
    PF00930F    414-459
    PF00930G    495-532
    PF00930H    532-574
    PF00930I    610-637
    PF00930J    659-679
***** IPB002471 (Prolyl endopeptidase family serine active site) with a combined E-value of 2.3e-12.
    IPB002471B    568-598


 ProDom Summary:  Protein Domain Search
Residues 591-714 are identical to a (DIPEPTIDYL HYDROLASE PROTEIN IV PEPTIDASE PROTEASE) protein domain (PD003048 which is seen in O66223_PORGI.

Residues 91-380 are identical to a (DIPEPTIDYL IV HYDROLASE PROTEASE) protein domain (PD003086 which is seen in O66223_PORGI.

Residues 1-90 are identical to a (IV DIPEPTIDYL PEPTIDASE DPP) protein domain (PD152979 which is seen in O66223_PORGI.

Residues 384-589 are identical to a (DIPEPTIDYL HYDROLASE IV PROTEIN PEPTIDASE PROTEASE) protein domain (PD001060 which is seen in O66223_PORGI.


Paralogs:  Local Blast Search
Residues 147 to 723 share 31% identity with PG1196.
Residues 394 to 700 share 23% identity with PG0652, a dipeptidyl peptidase IV.

Pfam Summary:  Pfam Search
Residues 26 to 518 (E-value = 6e-233) place PG0459 in the DPPIV_N family which is described as Dipeptidyl peptidase IV (DPP IV) N-terminal region (PF00930)
Residues 522 to 598 (E-value = 5.8e-19) place PG0459 in the Peptidase_S9 family which is described as Prolyl oligopeptidase family (PF00326)

 Structural Feature(s):
Feature Type  Start  Stop
cleavable signal  
1  
19
gram negative signal  
1  
19

Top PDB Hits:
-48% similar to PDB:1Z68 Crystal Structure Of Human Fibroblast Activation Protein alpha (E_value = 4.6E_89);
-48% similar to PDB:2GBC Native DPP-IV (CD26) from Rat (E_value = 4.5E_84);
-48% similar to PDB:2GBF rat dpp-IV with alkynyl cyanopyrrolidine #1 (E_value = 4.5E_84);
-48% similar to PDB:2GBG rat DPP-IV with alkynyl cyanopyrrolidine #2 (E_value = 4.5E_84);
-48% similar to PDB:2GBI rat DPP-IV with xanthine inhibitor 4 (E_value = 4.5E_84);

Gene Protein Sequence:
MKRPVIILLLGIVTMCAMAQTGNKPVDLKEITSGMFYARSAGSGIRSMPD
GEHYTEMNRERTAIIRYNYASGKAVDTLFSVERARECPFKQIQNYEVSST
GHHILLFTDMESIYRHSYRAAVYDYDVRRNLVKPLSEHVGKVMIPTFSPD
GRMVAFVRDNNIFIKKFDFDTEVQVTTDGQINSILNGATDWVYEEEFGVT
NLMSWSADNAFLAFVRSDESAVPEYRMPMYEDKLYPEDYTYKYPKAGEKN
STVSLHLYNVADRNTKSVSLPIDADGYIPRIAFTDNADELAVMTLNRLQN
DFKMYYVHPKSLVPKLILQDMNKRYVDSDWIQTLKFTTGGGFAYVSEKDG
FAHIYLYDNKGVMHRRITSGNWDVTKLYGVDASGTVFYQSAEESPIRRAV
YAIDAKGRKTKLSLNVGTNDALFSGNYAYYINTYSSAATPAVVSVFRSKG
AKELRTLEDNVALRERLKAYRYNPKEFTTIKTQSGLELNAWIVKPIDFDP
SRHYPVLMVQYSGPNSQQVLDRYSFDWEHYLASKGYVVACVDGRGTGARG
EEWRKCTYMQLGVFESDDQIAAATAIGQLPYVDAARIGIWGWSYGGYTTL
MSLCRGNGTFKAGIAVAPVADWRFYDSVYTERFMRTPKENASGYKMSSAL
DVASQLQGNLLIVSGSADDNVHLQNTMLFTEALVQANIPFDMAIYMDKNH
SIYGGNTRYHLYTRKAKFLFDNL

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAAAGACCGGTAATAATTCTGCTACTTGGCATCGTAACCATGTGTGC
CATGGCTCAGACGGGGAACAAACCCGTAGATTTGAAAGAAATCACGAGCG
GAATGTTTTATGCTCGCAGTGCAGGGAGTGGAATACGCTCCATGCCGGAT
GGAGAACACTATACGGAGATGAACCGTGAGCGAACGGCTATCATTCGCTA
CAATTATGCCTCCGGCAAGGCGGTAGATACGCTCTTCAGTGTCGAACGAG
CACGCGAATGCCCGTTTAAACAAATACAGAACTACGAGGTAAGCAGTACC
GGACATCATATTTTGCTCTTTACGGATATGGAGAGCATCTATCGGCATTC
GTATCGTGCTGCCGTCTACGACTATGATGTTCGCCGCAATTTGGTAAAAC
CACTGAGCGAGCATGTCGGCAAAGTGATGATCCCTACATTCAGCCCCGAT
GGCCGGATGGTAGCGTTCGTCAGAGACAATAACATCTTTATCAAGAAATT
CGATTTCGACACGGAAGTACAGGTTACTACCGATGGGCAGATCAACTCTA
TTCTTAACGGAGCGACGGATTGGGTGTACGAAGAAGAGTTCGGTGTGACC
AATCTGATGAGCTGGAGTGCGGACAATGCTTTTCTGGCTTTTGTGCGCAG
CGATGAATCTGCCGTCCCCGAATATCGAATGCCTATGTATGAGGACAAAC
TTTATCCCGAAGACTATACCTATAAGTATCCCAAGGCAGGGGAGAAGAAT
AGTACCGTCTCCCTGCATCTCTATAATGTGGCGGATCGGAATACCAAGTC
GGTAAGCCTGCCGATCGATGCGGATGGATATATTCCCCGAATTGCTTTCA
CGGACAACGCGGATGAGTTGGCTGTCATGACACTCAACCGTTTGCAGAAC
GACTTCAAAATGTACTATGTGCATCCGAAGAGTCTCGTCCCCAAGCTGAT
ACTACAGGATATGAACAAGCGATATGTGGATAGCGATTGGATTCAGACCT
TGAAGTTTACGACCGGAGGTGGATTCGCCTATGTGAGCGAAAAGGATGGG
TTTGCCCATATCTATCTCTACGATAATAAGGGGGTAATGCACCGTCGGAT
TACCTCAGGAAATTGGGATGTGACCAAACTGTACGGAGTGGATGCTTCGG
GAACGGTCTTCTACCAGTCGGCGGAAGAAAGCCCCATCCGTCGAGCTGTC
TATGCCATAGATGCCAAAGGCAGGAAAACAAAGCTCAGCCTGAATGTAGG
CACGAATGATGCTCTCTTTAGTGGCAATTATGCATACTATATTAACACGT
ATAGCAGTGCTGCTACCCCAGCGGTGGTTTCGGTATTCAGAAGCAAAGGT
GCCAAAGAGCTGCGTACACTGGAGGATAACGTTGCTCTCCGTGAACGGCT
GAAAGCCTATCGTTACAACCCGAAGGAGTTTACCACTATCAAAACTCAAT
CGGGTCTTGAACTGAATGCCTGGATCGTGAAGCCTATTGATTTCGATCCC
TCTCGCCACTATCCTGTCCTGATGGTACAGTATAGCGGTCCCAACTCCCA
GCAGGTATTGGATCGCTATTCATTCGATTGGGAACACTACCTTGCATCGA
AAGGTTACGTCGTGGCATGTGTGGATGGGCGTGGCACCGGTGCTCGCGGC
GAAGAATGGCGCAAGTGTACCTACATGCAACTCGGTGTATTCGAAAGCGA
TGATCAGATAGCAGCGGCCACTGCTATAGGACAGCTGCCCTATGTGGATG
CAGCTCGTATCGGCATATGGGGGTGGAGCTATGGCGGCTATACCACACTA
ATGAGTTTGTGTCGGGGAAATGGTACATTCAAAGCGGGGATAGCCGTTGC
TCCTGTGGCAGACTGGCGTTTCTACGATTCGGTTTACACCGAACGCTTCA
TGCGTACACCCAAGGAGAATGCTTCCGGATACAAGATGTCTTCTGCTCTT
GATGTGGCAAGCCAATTACAAGGAAACCTCTTGATCGTAAGCGGATCGGC
AGACGACAATGTTCATCTTCAGAACACGATGCTTTTTACAGAGGCACTGG
TTCAGGCCAATATCCCCTTCGACATGGCTATCTATATGGACAAGAACCAT
AGTATATACGGGGGGAATACCCGCTATCATCTCTATACTCGCAAAGCAAA
GTTTTTGTTCGACAATCTT

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