LANL Gene ID: PG0533
GenBank Locus Tag: PG0591
DNA Molecule Name:
1
GenBank ID:
34540399
Gene Name:
ISPg5
Definition:
ISPg5 transposase
Cellular Location:
Cytoplasm [Evidence]
Gene Start:
648625
Gene Stop:
649560
Gene Length:
936
Molecular Weight*:
36163
pI*:
9.90
Net Charge*:
14.47
EC:
Functional Class:
Transposon functions
Gene Ontology:
Biological process
GO:0015074 DNA integration
Molecular function
GO:0003677 DNA binding
Pathway: pathway table
Primary Evidence:
Califano,J.V., Kitten,T., Lewis,J.P., Macrina,F.L., Fleischmann,R.D., Fraser,C.M.,Duncan,M.J. and Dewhirst,F.E. Characterization of Porphyromonas gingivalis insertion
sequence-like element ISPg5.
Infect. Immun. 68 (9), 5247-5253 (2000)
Comment:
One of eleven ISPg5 elements in the genome (see Paralogs). Califano et al. report considerable strain diversity of these (Infect. Immun. 68 (9), 5247-5253 (2000)).
Member of the IS3 family of insertion elements. See PG0532 for associated ORF.
TIGR ID: PG0591
Human Oral Microbiome Database:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
PG0533 is equivalent to the previously reported gb|AAF69128.1| in GenBANK.
Numerous significant hits in gapped BLAST to transposase proteins; e.g. residues 91-213 are 56% similar to transposase homolog (U67062) of Bacteriodes thetaiotaomicrom, residues 64-287 are 32% similar to InsB (AF153317) of Shigella dysenteriae, residues 64-268 are 33% similar to Hp1 (AF081284) of E.coli.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
InterPro Summary: InterProScan
| InterPro IPR001584 Domain 
|
| ||||||||||
| noIPR unintegrated |
| ||||||||||
![[110-273]T 5.4e-31 PF00665 rve](http://www.ebi.ac.uk/InterProScan/images/H.gif){kind=small}
![[110-276]T 18.438 PS50994 INTEGRASE](http://www.ebi.ac.uk/InterProScan/images/M.gif){kind=small}
![[113-281]T 1.2e-06 G3DSA:3.30.420.10 no description](http://www.ebi.ac.uk/InterProScan/images/G.gif){kind=small}
COGS Summary: COGS Search
No hit to the COGs database.
Blocks Summary: Blocks Search
***** PF01836 (Transposase) with a combined E-value of 4.3e-23.
PF01836B 103-154
PF01836C 181-190
PF01836D 215-222
PF01836E 253-272
ProDom Summary: Protein Domain Search
No significant hit to the ProDom database.
Paralogs: Local Blast Search
PG0533 is paralogously related to PG1861, PG1491, PG0391, PG0418, PG1436, PG1796, PG0034, PG0007, PG1247 and PG0841.
Pfam Summary: Pfam Search
Residues 110 to 273 (E-value = 4.4e-31) place PG0533 in the rve family which is described as Integrase core domain (PF00665)
Structural Feature(s):
| Feature Type | Start | Stop |
| No predicted structural features. |
Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).
Gene Protein Sequence:
LLGFSRQAFYKRHLNDLAQHEEDVLCSSIIQYCWHLRQAEHLPQAGFREL
MVLCQQYFGPKFTLGRDRFCALLRRHGMMLRKRSVRPRTTNSRHRLYKYE
DLLNTEPKFVPQRPGELLVADITYVAYQDGFAYLSLLTDAYSRCIVGYCL
HPTLEVEGCLNALHQAFAFYDQHQIDTSNMIHHSDRGIQYAGKSYTDLLH
GRGCRISMTQTGDPLHNALAERMNNTLKNSWHISSSKQSFDQALLSVEQA
VRMYNEARPHQALGAKTPMQVIAPESKNPLLTRREHGPEIAPELYRRMNV
RQRANFARVNRN
Gene Nucleotide Sequence: Sequence Viewer
CTGCTTGGCTTTAGCCGTCAGGCCTTTTATAAACGTCATCTCAACGATTT
AGCACAACATGAGGAGGATGTCCTATGCAGTAGTATCATCCAATATTGTT
GGCACTTAAGACAAGCAGAGCACCTACCTCAAGCCGGCTTCCGAGAGCTC
ATGGTGCTTTGTCAGCAATACTTCGGCCCCAAGTTCACGCTTGGGCGCGA
TCGCTTTTGCGCCCTACTCAGACGCCATGGTATGATGCTGCGCAAACGGT
CTGTGCGCCCTCGGACAACCAACTCTCGGCATAGGCTCTACAAGTATGAA
GACCTGCTCAATACAGAGCCTAAGTTTGTGCCTCAAAGACCTGGGGAACT
CCTTGTAGCAGACATCACCTACGTGGCTTACCAAGATGGGTTTGCCTATC
TCTCCCTACTGACCGATGCCTATAGTCGCTGTATCGTAGGGTATTGCTTA
CATCCTACCCTAGAGGTTGAAGGCTGTTTGAATGCTCTACATCAAGCCTT
TGCTTTCTACGATCAGCATCAAATTGATACGAGCAATATGATTCATCATA
GCGACCGAGGCATTCAGTATGCCGGTAAGAGCTACACCGATCTGTTGCAT
GGGCGAGGCTGTCGCATCAGCATGACTCAGACGGGAGATCCTCTGCATAA
TGCATTGGCTGAGCGGATGAACAATACGCTCAAGAACTCGTGGCACATCT
CCTCTTCGAAGCAGTCTTTTGATCAAGCACTCCTTTCTGTAGAACAAGCC
GTGCGCATGTACAACGAGGCGCGTCCACATCAAGCTCTAGGGGCGAAAAC
GCCTATGCAAGTCATTGCGCCAGAGTCTAAAAATCCGTTACTAACGAGGA
GGGAGCATGGGCCAGAGATTGCCCCCGAGCTATACCGAAGGATGAATGTC
AGACAAAGAGCTAACTTTGCTCGTGTCAACCGAAAT