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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0456 IGR0457 IGR0451 IGR0449 IGR0455 IGR0454 IGR0453 IGR0458 IGR0450 IGR0452 guaA, - PG0531 rl31, - PG0534 rpsR, - PG0538 rps6,rpsF, - PG0537 ISPg5, - PG0532 rl9,rpL9, - PG0540 sigA,rspD,whiG,rpoD, - PG0536 ISPg5, - PG0533 htrA, - PG0535 PG0541 guaA, - PG0531 rl31, - PG0534 rpsR, - PG0538 rps6,rpsF, - PG0537 ISPg5, - PG0532 rl9,rpL9, - PG0540 sigA,rspD,whiG,rpoD, - PG0536 ISPg5, - PG0533 htrA, - PG0535 PG0541 Type: IS element, Name: ISPg5 - 8 guaA, - PG0531 rl31, - PG0534 rps6,rpsF, - PG0537 ISPg5, - PG0532 rl9,rpL9, - PG0540 sigA,rspD,whiG,rpoD, - PG0536 ISPg5, - PG0533 htrA, - PG0535 PG0541 rpsR, - PG0538


LANL Gene ID: PG0535

GenBank Locus Tag: PG0593

DNA Molecule Name:
1  

GenBank ID:
34540401

Gene Name:
htrA  

Definition:
periplasmic serine protease

Cellular Location:
Outer membrane, Periplasm, Cytoplasm [Evidence]

Gene Start:
650503

Gene Stop:
651996

Gene Length:
1494

Molecular Weight*:
52766

pI*:
8.80

Net Charge*:
1.99

EC:
 

Functional Class:
Protein fate; Degradation of proteins, peptides and glycopeptides  

Gene Ontology:
Biological process
  GO:0006508    proteolysis

Molecular function
  GO:0004252    serine-type endopeptidase activity
  GO:0005515    protein binding


Pathway: pathway table

Primary Evidence:
Roy F, Vanterpool E, Fletcher HM., HtrA in Porphyromonas gingivalis can regulate growth and gingipain activity under stressful environmental conditions., Microbiology. 2006 Nov;152(Pt 11):3391-8.
PMID: 17074908

Yuan L, Rodrigues PH, Bélanger M, Dunn WA Jr, Progulske-Fox A., Porphyromonas gingivalis htrA is involved in cellular invasion and in vivo survival., Microbiology. 2008 Apr;154(Pt 4):1161-9.
PMID: 18375808

Yuan L, Hillman JD, Progulske-Fox A., Microarray analysis of quorum-sensing-regulated genes in Porphyromonas gingivalis.
Infect Immun. 2005 Jul;73(7):4146-54. PMID: 15972504

Secondary Evidence:
Lipinska, B., O. Fayet, L. Baird, C. Georgopoulos. 1989. Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures. J. Bacteriol. 171:1574-1584. PubMed: 2537822.

Lipinska, B., M. Zylicz, C. Georgopoulos 1990. The HtrA (DegP)
protein, essential for Escherichia coli survival at high temperatures, is an endopeptidase. J. Bacteriol. 172:1791-1797. PubMed: 2180903.

Comment:
In E. coli, this enzyme is induced by heat shock, when it is required for degradation of damaged proteins.

In E. coli, htrA is transcribed from a sigma-E promoter. HtrA is a periplasmic endoprotease which is indispensable at high temperatures and which may function to degrade transiently degraded substrates in periplasm. See also degQ.

Serine proteases belong to peptidase family S2C; also known as the HtrA/HhoA/HhoB family.

TIGR ID: PG0593

Proteomic Data: Proteomic Data Search
TIGR AnnotationHtrA protein, GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG05930.400.0270.230.192.5200.230.19
LANLPG05351.321.175.733.31
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Several significant hits with gapped blast to serine proteases or HtrA-like proteins from, i.e., Pseudomonas aeruginosa (1184684), Haemophilus influenzae (1170411; 2935166; 2935168), Chlamydia trachomatis (3329293), Chlamydia muridarum (7190248), among others.

This protein is similar to CT823, a predicted periplasmic serine protease, and to BT1312.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540401|ref|NP_904880.1|  htrA protein [Porphyromonas gi...   873   0.0  
 gi|154495209|ref|ZP_02034214.1|  hypothetical protein PARMER...   468   e-130
 gi|150007021|ref|YP_001301764.1|  serine protease [Parabacte...   466   e-129
 gi|156858344|gb|EDO51775.1|  hypothetical protein BACUNI_043...   402   e-110
 gi|150005265|ref|YP_001300009.1|  serine protease precursor ...   390   e-107
 gi|153808301|ref|ZP_01960969.1|  hypothetical protein BACCAC...   389   e-106
 gi|29346722|ref|NP_810225.1|  serine protease precursor [Bac...   389   e-106
 gi|156111975|gb|EDO13720.1|  hypothetical protein BACOVA_006...   388   e-106
 gi|53714036|ref|YP_100028.1|  serine protease precursor [Bac...   379   e-103
 gi|110636479|ref|YP_676686.1|  serine protease [Cytophaga hu...   353   2e-95


InterPro Summary:  InterProScan

InterPro
IPR001254
Domain
Peptidase S1 and S6, chymotrypsin/Hap
PF00089 [105-286]T 6.30000946287267E-21 PF00089 Trypsin Trypsin
SM00020 [65-286]T 8.9 SM00020 Tryp_SPc Tryp_SPc
InterPro
IPR001478
Domain
PDZ/DHR/GLGF
SM00228 [298-380]T 2.29999999999998E-6 SM00228 PDZ[406-485]T 0.00540000000000002 SM00228 PDZ PDZ
SSF50156 [297-393]T 1.6E-21 SSF50156 PDZ[404-498]T 1.1E-8 SSF50156 PDZ PDZ
InterPro
IPR001940
Family
Peptidase S1C, HrtA/DegP2/Q/S
PR00834 [129-141]T 1.9E-52 PR00834 PROTEASES2C[150-170]T 1.9E-52 PR00834 PROTEASES2C[190-214]T 1.9E-52 PR00834 PROTEASES2C[230-247]T 1.9E-52 PR00834 PROTEASES2C[252-269]T 1.9E-52 PR00834 PROTEASES2C[338-350]T 1.9E-52 PR00834 PROTEASES2C PROTEASES2C
InterPro
IPR009003
Domain
Peptidase, trypsin-like serine and cysteine
SSF50494 [62-294]T 4.5E-73 SSF50494 Pept_Ser_Cys Pept_Ser_Cys
InterPro
IPR011782
Family
Peptidase S1C, Do
TIGR02037 [64-494]T 0.0 TIGR02037 degP_htrA_DO degP_htrA_DO
noIPR
unintegrated
unintegrated
G3DSA:2.30.42.10 [316-388]T 9.10000425645564E-6 G3DSA:2.30.42.10 G3DSA:2.30.42.10[404-498]T 3.69999844802225E-8 G3DSA:2.30.42.10 G3DSA:2.30.42.10 G3DSA:2.30.42.10
G3DSA:2.40.10.10 [87-173]T 1.1000015067164302E-21 G3DSA:2.40.10.10 G3DSA:2.40.10.10[181-302]T 1.60000240695986E-35 G3DSA:2.40.10.10 G3DSA:2.40.10.10 G3DSA:2.40.10.10
PTHR22939 [76-94]T 0.0 PTHR22939 PTHR22939[117-487]T 0.0 PTHR22939 PTHR22939 PTHR22939
PTHR22939:SF10 [76-94]T 0.0 PTHR22939:SF10 PTHR22939:SF10[117-487]T 0.0 PTHR22939:SF10 PTHR22939:SF10 PTHR22939:SF10


COGS Summary:  COGS Search
BeTs to 13 clades of COG0265
COG name: Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain
Functional Class: O
The phylogenetic pattern of COG0265 is --t-yqvCEBRHuj--oLinX
Number of proteins in this genome belonging to this COG is 3
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 185-308 are 57% similar to a (PROTEASE SERINE PROTEIN PERIPLASMIC) protein domain (PD001397 which is seen in HTOA_HAEIN.

Residues 189-456 are 24% similar to a (PROTEIN SPC98-TOM70 INTERGENIC REGION) protein domain (PD145944 which is seen in YNM3_YEAST.

Residues 120-184 are 60% similar to a (PROTEASE SERINE PROTEIN PERIPLASMIC) protein domain (PD001340 which is seen in Q57155_PSEAE.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
Residues 105 to 286 (E-value = 4e-21) place PG0535 in the Trypsin family which is described as Trypsin (PF00089)

 Structural Feature(s):
Feature Type  Start  Stop
cleavable signal  
1  
27
gram negative signal  
1  
27

Top PDB Hits:
-54% similar to PDB:1KY9 Crystal Structure of DegP (HtrA) (E_value = 1.2E_66);
-59% similar to PDB:1SOT Crystal Structure of the DegS stress sensor (E_value = 2.6E_48);
-59% similar to PDB:1SOZ Crystal Structure of DegS protease in complex with an activating peptide (E_value = 2.6E_48);
-59% similar to PDB:1VCW Crystal structure of DegS after backsoaking the activating peptide (E_value = 2.6E_48);
-59% similar to PDB:1TE0 Structural analysis of DegS, a stress sensor of the bacterial periplasm (E_value = 3.7E_47);

Gene Protein Sequence:
MNKVWKYVTGAFGIAAVSAVASVATVAALGHYRGESFITDGTENEFGFKQ
TSYTTTSGASAALPNLVGAAEASVHAVVHIKVESEQRMDSQQYFDPFEFF
FGGESRNFQRPQTRQVVGYGSGVIISTDGYIITNNHVVKGAKEMTVTLND
NRTFKAKLIGSDATTDIALLKVDAKGLPTIPFGDSDKLRVGEWVLAVGNP
FNLTSTVTAGIVSAKGRSTQQVARGGSLQIESFIQTDAAVNSGNSGGALV
NDRGELIGINTMIYSQTGNYAGYSFAVPISIAAKVVADIKQYGTVQRAVL
GIAGGDISDEAVKEYDLKVREGALVADFAEVSAAISAGMQKGDVITAVEG
KQIKSFPQLQEAISRYRPGDKVKLTINRKGATKELTVTLKNNEGSTSVIT
GESTGNVLGASFKDLSAEQMRSYGVSYGVEVTSVSSGKFKDAGIKKGFII
LSINRQPVSSGADVSDIVREAGQSRSGRLIIVRGFYPDGNIRTFEVEL

Gene Nucleotide Sequence:  Sequence Viewer
ATGAACAAAGTGTGGAAATACGTGACGGGAGCCTTTGGCATAGCAGCCGT
CAGCGCAGTAGCATCGGTGGCAACAGTGGCTGCTTTGGGACACTACCGAG
GTGAATCTTTCATAACCGATGGTACGGAAAACGAATTCGGATTCAAGCAG
ACATCTTACACAACGACATCCGGAGCCTCCGCCGCTCTGCCCAATTTGGT
CGGAGCTGCAGAAGCATCGGTACACGCCGTAGTACACATCAAAGTGGAGT
CCGAGCAGCGAATGGACTCTCAGCAGTACTTCGATCCGTTCGAATTCTTC
TTCGGAGGCGAATCCAGAAACTTCCAGCGTCCCCAAACACGGCAGGTAGT
AGGCTACGGTTCGGGGGTGATCATCAGCACAGACGGATATATCATCACGA
ATAATCATGTAGTAAAGGGAGCCAAAGAAATGACGGTAACGCTCAATGAC
AATCGTACCTTCAAGGCCAAACTGATCGGTAGCGATGCCACGACCGACAT
AGCCTTGCTGAAAGTAGATGCCAAGGGGCTGCCGACAATTCCTTTCGGCG
ACTCTGACAAGCTTCGTGTGGGAGAGTGGGTATTGGCCGTAGGCAATCCT
TTTAATCTTACTTCCACGGTAACGGCCGGCATCGTCAGCGCAAAAGGTCG
CTCCACTCAGCAGGTGGCCAGAGGCGGTTCGCTCCAGATAGAATCTTTTA
TTCAGACGGATGCTGCCGTCAATTCAGGCAACAGCGGCGGTGCGTTGGTG
AATGATCGTGGCGAACTGATCGGTATCAATACGATGATCTACAGCCAAAC
GGGCAACTATGCCGGCTACTCATTTGCCGTACCTATTTCCATAGCGGCGA
AAGTGGTAGCCGACATCAAGCAGTACGGTACCGTACAGCGCGCCGTGCTG
GGCATTGCCGGCGGAGATATATCGGACGAAGCAGTCAAAGAATACGATCT
GAAGGTGCGCGAGGGTGCTCTCGTAGCAGACTTTGCCGAAGTCAGTGCAG
CTATCTCTGCCGGTATGCAGAAGGGGGATGTGATCACCGCCGTAGAAGGC
AAACAGATAAAGAGTTTCCCACAACTGCAAGAAGCCATCAGCCGCTACAG
ACCGGGAGACAAAGTGAAGCTGACCATCAATCGTAAGGGAGCGACCAAGG
AACTGACCGTCACGCTCAAGAACAATGAAGGAAGTACTTCGGTAATCACC
GGCGAATCGACCGGAAATGTATTGGGAGCATCATTCAAAGACCTCAGTGC
CGAACAAATGCGATCCTATGGTGTTTCTTATGGTGTAGAGGTAACCTCTG
TAAGCTCGGGGAAATTCAAGGATGCAGGTATCAAGAAAGGATTCATCATC
CTTTCGATCAACCGCCAACCGGTAAGCTCGGGTGCCGATGTCAGCGACAT
CGTTCGTGAGGCCGGACAGAGCCGATCGGGTCGCTTGATCATCGTGCGTG
GGTTTTATCCGGACGGTAATATCCGGACCTTCGAAGTAGAACTA

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