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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0475 IGR0470 IGR0473 IGR0474 IGR0469 IGR0471 IGR0476 IGR0472 PG0555 PG0562 PG0559 ahpC,ahpF, - PG0556 PG0563 tpiA,tpiS, - PG0561 PG0554 PG0560 ahpF, - PG0557 lon, - PG0558 PG0555 PG0562 PG0559 ahpC,ahpF, - PG0556 PG0563 tpiA,tpiS, - PG0561 PG0554 PG0560 ahpF, - PG0557 lon, - PG0558 PG0559 ahpC,ahpF, - PG0556 PG0563 tpiA,tpiS, - PG0561 PG0554 PG0560 ahpF, - PG0557 lon, - PG0558 PG0555 PG0562


LANL Gene ID: PG0558

GenBank Locus Tag: PG0620

DNA Molecule Name:
1  

GenBank ID:
34540426

Gene Name:
lon  

Definition:
ATP-dependent protease LA

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
673104

Gene Stop:
675533

Gene Length:
2430

Molecular Weight*:
91780

pI*:
7.30

Net Charge*:
2.31

EC:
3.4.21.53  

Functional Class:
Protein fate; Degradation of proteins, peptides and glycopeptides  

Gene Ontology:
Biological process
  GO:0006508    proteolysis
  GO:0006510    ATP-dependent proteolysis

Molecular function
  GO:0000166    nucleotide binding
  GO:0004176    ATP-dependent peptidase activity
  GO:0004252    serine-type endopeptidase activity
  GO:0005524    ATP binding
  GO:0017111    nucleoside-triphosphatase activity


Pathway: pathway table

Secondary Evidence:
Fischer H, Glockshuber R. A point mutation within the ATP-binding site inactivates both catalytic functions of the ATP-dependent protease La (Lon) from Escherichia coli. FEBS Lett. Dec 12;356(1):101-3. PubMed: 7988699.

2) Chin DT, Goff SA, Webster T, Smith T, Goldberg AL. Sequence of the lon gene in Escherichia coli. A heat-shock gene which encodes the ATP-dependent protease La. J Biol Chem 1988 Aug 25;263(24):11718-28. PubMed: 3042779.

Comment:
TIGR ID: PG0620

Proteomic Data: Proteomic Data Search
TIGR AnnotationATP-dependent protease La, GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG06200.710.00032.0500.691e-052.050
LANLPG05581.644.151.611.65
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Numerous significant hits with gapped BLAST to ATP-dependent
proteases; e.g. residues 8-777 are 75% similar to ATP-dependent
protease (AE000680) of Aquifex aeolicus, residues 4-778 are 44%
similar to ATP-dependent protease (Z99118) of Bacillus subtilis,
residues 6-773 are 41% similar to ATP-dependent protease (AE001588) of Chlamydophila pneumoniae, residues 3-773 are 40%
similar to ATP-dependent protease (AE001307) of Chlamydia trachomatis.

This sequence is similar to BT0837.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540426|ref|NP_904905.1|  ATP-dependent protease La [Por...  1525   0.0  
 gi|154494855|ref|ZP_02033860.1|  hypothetical protein PARMER...  1085   0.0  
 gi|150008488|ref|YP_001303231.1|  ATP-dependent protease [Pa...  1069   0.0  
 gi|156862180|gb|EDO55611.1|  hypothetical protein BACUNI_006...   977   0.0  
 gi|153806354|ref|ZP_01959022.1|  hypothetical protein BACCAC...   974   0.0  
 gi|156110323|gb|EDO12068.1|  hypothetical protein BACOVA_025...   972   0.0  
 gi|53713593|ref|YP_099585.1|  ATP-dependent protease [Bacter...   970   0.0  
 gi|150005673|ref|YP_001300417.1|  ATP-dependent protease [Ba...   923   0.0  
 gi|149278727|ref|ZP_01884862.1|  ATP-dependent protease La [...   885   0.0  
 gi|89890783|ref|ZP_01202292.1|  class III heat shock DNA-bin...   867   0.0


InterPro Summary:  InterProScan

InterPro
IPR001984
Family
Peptidase S16, Lon protease
PR00830 [358-377]T 3.6e-52 PR00830 ENDOLAPTASE[597-613]T 3.6e-52 PR00830 ENDOLAPTASE[676-695]T 3.6e-52 PR00830 ENDOLAPTASE[706-725]T 3.6e-52 PR00830 ENDOLAPTASE[729-747]T 3.6e-52 PR00830 ENDOLAPTASE ENDOLAPTASE
InterPro
IPR003111
Domain
Peptidase S16, lon N-terminal
PF02190 [9-97]T 8.6e-17 PF02190 LON LON
SM00464 [9-205]T 5.4e-38 SM00464 LON LON
InterPro
IPR003593
Domain
AAA+ ATPase, core
SM00382 [350-503]T 3.8e-13 SM00382 AAA AAA
InterPro
IPR003959
Domain
AAA ATPase, core
PF00004 [353-548]T 1.4e-47 PF00004 AAA AAA
InterPro
IPR004815
Family
Peptidase S16, ATP-dependent protease La
TIGR00763 [11-774]T 0 TIGR00763 lon: ATP-dependent protease La lon: ATP-dependent protease La
InterPro
IPR008268
Active_site
Peptidase S16, active site
PS01046 [679-687]T 8e-5 PS01046 LON_SER LON_SER
InterPro
IPR008269
Domain
Peptidase S16, lon C-terminal
PF05362 [571-776]T 1.2e-115 PF05362 Lon_C Lon_C
noIPR
unintegrated
unintegrated
G3DSA:1.10.8.60 [494-586]T 2.2e-26 G3DSA:1.10.8.60 no description no description
G3DSA:3.40.50.300 [310-490]T 3.7e-31 G3DSA:3.40.50.300 no description no description
PTHR10046 [206-786]T 2e-244 PTHR10046 ATP DEPENDENT LON PROTEASE FAMILY MEMBER ATP DEPENDENT LON PROTEASE FAMILY MEMBER


COGS Summary:  COGS Search
BeTs to 11 clades of COG0466
COG name: ATP-dependent Lon protease, bacterial type
Functional Class: O
The phylogenetic pattern of COG0466 is ----yqv-eb-hujgpOlinx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** IPB001984 (ATP-dependent serine proteases, Lon family) with a combined E-value of 1.2e-211.
    IPB001984A    220-245
    IPB001984B    276-303
    IPB001984C    304-358
    IPB001984D    377-426
    IPB001984E    436-475
    IPB001984F    480-507
    IPB001984G    534-554
    IPB001984H    598-624
    IPB001984I    684-738
***** IPB001939 (AAA-protein (ATPases associated with various cellular activities)) with a combined E-value of 3.6e-06.
    IPB001939B    351-372


 ProDom Summary:  Protein Domain Search
Residues 128-241 are 32% similar to a (PROTEASE HYDROLASE SERINE ATP-BINDING ATP-DEPENDENT LA) protein domain (PD002364 which is seen in LON2_MYXXA.

Residues 627-759 are 54% similar to a (PROTEASE ATP-BINDING HYDROLASE SERINE PROTEIN) protein domain (PD001169 which is seen in LON_VIBPA.

Residues 125-280 are 29% similar to a (PROTEIN COILED COIL REPEAT FILAMENT HEPTAD PATTERN CHAIN) protein domain (PD000002 which is seen in LON1_MYXXA.

Residues 4-113 are 38% similar to a (PROTEASE ATP-DEPENDENT LA HYDROLASE SERINE ATP-BINDING) protein domain (PD003709 which is seen in LON2_MYXXA.

Residues 9-224 are 21% similar to a (HYPOTHETICAL 24.5 KD PROTEIN) protein domain (PD111918 which is seen in Q55701_SYNY3.

Residues 296-560 are 60% similar to a (PROTEIN ATP-BINDING PROTEASE SUBUNIT REGULATORY HOMOLOG) protein domain (PD000092 which is seen in LON1_BACSU.


Paralogs:  Local Blast Search
No paralogs found in P. gingivalis.

Pfam Summary:  Pfam Search
Residues 9 to 205 (E-value = 6.7e-12) place PG0558 in the LON family which is described as ATP-dependent protease La (LON) domain (PF02190)
Residues 353 to 548 (E-value = 1.7e-44) place PG0558 in the AAA family which is described as ATPase family associated with various cellular activities (AAA) (PF00004)
Residues 571 to 776 (E-value = 1e-115) place PG0558 in the Lon_C family which is described as Lon protease (S16) C-terminal proteolytic domain (PF05362)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
12  
28
coil-coil  
521  
564
transmembrane  
599  
615
non-globular  
669  
810

Top PDB Hits:
-70% similar to PDB:1RR9 Catalytic domain of E.coli Lon protease (E_value = 1.4E_46);
-70% similar to PDB:1RRE Crystal structure of E.coli Lon proteolytic domain (E_value = 1.4E_46);
-59% similar to PDB:1X37 Structure of Bacillus subtilis Lon protease SSD domain (E_value = 3.4E_16);
-64% similar to PDB:2ANE Crystal structure of N-terminal domain of E.Coli Lon Protease (E_value = 9.4E_14);
-48% similar to PDB:1XHK Crystal structure of M. jannaschii Lon proteolytic domain (E_value = 1.8E_12);

Gene Protein Sequence:
VKEDHMQEEMPILALRNMILFPGVAMPIMVGREKSLKLIRYVEKKGVYFG
AVSQRDMDVEEPDRADLYDVGVVAEIIRVLEMPDGTTTAIVQGRQRFALQ
EITATEPFMKGRVKLLPDILPGKNKDHEFEALVSTIQDMSLKMMELMVER
PPRELILSMRRNKNPMYQINFASANISTSIAVKQELLEISKMKDRGYRLL
YLLHKELQVMELKASIQMKTREEMDKQQKEYFLQQQIKTIQEELGGNIND
IEVQELRTKAATMKWSSEVAETFEKELRKLERLHPQSPDYSVQMQYVQTI
ISLPWGVFSKDNFNLKRAQSVLDRDHFGLEKVKERIIEHLAVLKMKGDMK
SPIICLYGPPGVGKTSLGKSIAESLGRKYVRISLGGLHDEAEIRGHRRTY
IGAMCGRIIQSLQRAGTSNPVFVLDEIDKIDSDYKGDPSSALLEVLDPEQ
NNAFHDNYLDIDFDLSHVLFIATANSLSSISRPLLDRMELIDVSGYIIEE
KVEIAARHLIPKQLVEHGFRKNDIKFSKKTIEKLIDDYTRESGVRTLEKQ
IAAVIRKITKEAATNVVHTTKVEPSDLVTFLGAPRYTRDRYQGNGDAGVV
IGLAWTSVGGEILFIETSLHRGREPKLTLTGNLGDVMKESAVIALDYIRA
HSDELGISQEIFNNWQVHVHVPEGAIPKDGPSAGITMVTSLVSALTRRKV
RAGIAMTGEITLRGKVLPVGGIKEKILAAKRSGITEIILCEENRKDIEEI
NDIYLKGLKFHYVSNINEVLKEALLEEKVIDTTDIYSFGKKTEEEKTEKV
EKTEKKQRKK

Gene Nucleotide Sequence:  Sequence Viewer
GTGAAAGAAGATCATATGCAGGAGGAGATGCCGATTTTGGCTCTCCGCAA
TATGATTCTCTTCCCCGGTGTGGCCATGCCTATCATGGTCGGACGAGAAA
AATCACTCAAGCTGATCCGCTACGTGGAGAAGAAGGGTGTCTATTTCGGT
GCCGTTTCACAGCGCGATATGGATGTGGAGGAACCGGATCGTGCCGATTT
GTATGATGTCGGGGTCGTGGCTGAAATCATCAGGGTGCTGGAGATGCCCG
ATGGTACTACCACGGCTATCGTACAAGGCCGCCAGCGATTTGCACTGCAG
GAGATAACGGCTACGGAACCTTTTATGAAGGGTCGCGTGAAGCTGCTCCC
AGACATTCTCCCCGGGAAGAACAAAGATCACGAGTTCGAAGCACTCGTCT
CCACCATACAGGATATGAGTCTGAAGATGATGGAGCTGATGGTCGAGAGA
CCCCCTCGGGAGCTTATCCTCTCGATGAGACGGAACAAGAATCCGATGTA
TCAGATCAACTTTGCATCGGCCAATATATCCACGAGCATAGCCGTCAAGC
AAGAATTGCTGGAAATCAGCAAGATGAAGGATCGTGGCTATCGTCTTCTC
TACCTGTTGCACAAGGAGCTTCAGGTAATGGAACTGAAAGCTTCTATTCA
GATGAAGACGCGCGAGGAGATGGACAAGCAGCAGAAAGAGTACTTCCTGC
AGCAGCAAATCAAGACGATTCAGGAGGAACTGGGTGGCAATATAAACGAC
ATCGAGGTGCAGGAGCTGCGTACCAAAGCTGCTACGATGAAGTGGAGCAG
CGAGGTAGCAGAAACTTTCGAAAAAGAACTCCGCAAGCTGGAACGGCTAC
ATCCTCAATCGCCCGACTACTCCGTACAGATGCAGTATGTACAGACCATC
ATCAGTCTGCCGTGGGGCGTATTCAGTAAAGATAATTTCAACCTGAAGCG
TGCCCAATCCGTTTTGGATAGAGATCATTTCGGTCTTGAGAAAGTAAAAG
AGAGAATCATAGAGCATCTGGCTGTCCTCAAGATGAAAGGCGATATGAAG
TCGCCCATCATCTGTCTGTACGGCCCTCCGGGAGTGGGTAAGACCTCTCT
TGGCAAGAGCATTGCCGAGAGCTTGGGTCGTAAATACGTACGCATTTCTT
TAGGGGGCTTGCATGACGAAGCTGAGATTCGTGGACACCGACGTACCTAC
ATCGGTGCTATGTGCGGACGGATCATTCAGAGTTTGCAGCGAGCAGGTAC
ATCTAATCCCGTCTTTGTCCTCGACGAAATAGACAAGATCGACAGCGACT
ACAAGGGCGATCCTTCTTCTGCTTTGCTCGAAGTCCTTGATCCGGAGCAG
AACAATGCTTTTCATGACAATTATCTGGATATAGACTTCGACCTCAGTCA
TGTTCTTTTTATCGCTACGGCCAACAGTTTGAGCAGTATCTCTCGTCCTC
TTTTGGACAGAATGGAGCTGATCGATGTGAGCGGTTATATCATAGAAGAA
AAAGTGGAGATCGCTGCCCGCCATCTGATTCCAAAGCAACTTGTGGAGCA
TGGATTCAGAAAGAACGACATCAAGTTCTCCAAAAAAACCATAGAAAAGC
TGATCGACGACTACACCAGAGAGAGTGGAGTGCGTACGTTGGAGAAGCAG
ATCGCAGCTGTGATCCGTAAGATAACAAAGGAGGCCGCCACGAATGTGGT
ACATACTACCAAGGTGGAACCGTCCGACTTGGTTACTTTCTTGGGAGCAC
CCCGCTATACGCGCGATCGCTATCAAGGCAATGGCGATGCAGGCGTTGTG
ATCGGATTGGCTTGGACTTCGGTGGGAGGGGAGATCCTGTTCATCGAGAC
GAGTCTGCACCGCGGACGCGAACCGAAGCTCACGCTTACGGGTAATCTGG
GCGATGTGATGAAAGAGTCGGCCGTAATAGCCTTGGATTATATTCGTGCC
CATAGTGATGAGTTGGGCATCTCTCAGGAAATTTTCAATAATTGGCAGGT
ACACGTACATGTTCCCGAAGGTGCTATCCCGAAAGACGGTCCGAGTGCAG
GTATTACGATGGTGACTTCGCTGGTGTCGGCTCTTACCCGTCGAAAAGTG
CGGGCCGGTATTGCCATGACAGGAGAAATCACTCTAAGAGGAAAGGTGCT
TCCTGTAGGTGGAATCAAGGAGAAGATATTGGCTGCCAAACGTTCCGGTA
TCACGGAAATAATCCTGTGCGAAGAGAATCGCAAGGACATAGAGGAGATA
AACGATATCTATCTGAAGGGCTTGAAGTTCCACTATGTGTCCAACATTAA
CGAGGTGTTGAAAGAAGCTTTGCTCGAAGAGAAAGTCATCGATACCACCG
ATATATATTCCTTTGGCAAGAAGACCGAGGAGGAGAAAACCGAAAAAGTG
GAAAAAACGGAGAAGAAGCAAAGAAAGAAA

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