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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0526 IGR0531 IGR0529 IGR0525 IGR0527 IGR0530 IGR0528 PG0624 PG0628 4hbD, - PG0622 omp40, - PG0626 omp41,oprF, - PG0627 cat2, - PG0623 abfD, - PG0625 glgP, - PG0629 PG0624 PG0628 4hbD, - PG0622 omp40, - PG0626 omp41,oprF, - PG0627 cat2, - PG0623 abfD, - PG0625 glgP, - PG0629 Type: tandem, Name: tan5 - 213 PG0624 PG0628 4hbD, - PG0622 omp40, - PG0626 omp41,oprF, - PG0627 cat2, - PG0623 abfD, - PG0625 glgP, - PG0629


LANL Gene ID: PG0626

GenBank Locus Tag: PG0694

DNA Molecule Name:
1  

GenBank ID:
34540489

Gene Name:
omp40  

Definition:
outer membrane protein

Cellular Location:
Outer membrane, Cytoplasm [Evidence]

Gene Start:
750936

Gene Stop:
749797

Gene Length:
1140

Molecular Weight*:
42440

pI*:
8.00

Net Charge*:
2.56

EC:
 

Functional Class:
Cell envelope  

Gene Ontology:
Cellular component
  GO:0009279    cell outer membrane
  GO:0016021    integral to membrane


Pathway: pathway table

Primary Evidence:
Veith,P.D.,Talbo,G.H.,Slakeski,N.,Reynolds,E.C., Identification of a novel heterodimeric outer membrane protein of Porphyromonas gingivalis by two-dimensional gel electrophoresis and peptide mass fingerprinting, Eur. J. Biochem. (2001) 268, 4748-4757, PMID: 11532011.

Ross BC, Czajkowski L, Hocking D, Margetts M, Webb E, Rothel L, Patterson M, Agius C, Camuglia S, Reynolds E, Littlejohn T, Gaeta B, Ng A, Kuczek ES, Mattick JS, Gearing D, Barr IG, Identification of vaccine candidate antigens from a genomic analysis of Porphyromonas gingivalis, Vaccine 2001 Jul 20;19(30): 4135-42, PMID: 11457538.

Ross,B.C., Barr,I., Patterson,M., Agius,C., Rothel,L., Margetts,M., Hocking,D. and Webb,E., Porphyromonas gingivalis polypeptides and nucleic acids, Patent: Australia (AU 98/01023)-PCT 10-DEC-1998; CSL Ltd; 45 Poplar Road; R&D; Parkville, Victoria; Australia.

Comment:
Veith, et al.(2001) have shown that in strain W50 omp40 (PG0626) and omp41 (PG0627) are co-transcribed, and they form heterodimers through disulfide bonds to each other. They find that with the possible exception of the proteinases, Omp40/41 are the most abundant proteins associated with the outer membrane of W50. Like OmpA, they are heat-modifiable and can be proteolytically cleaved to yield two domains, shown below. The N-terminus of each mature protein is pyroGlu. These authors suspect that omp40/41 are porins.

Veith et al. divide the chain thus:
Signal peptide: residues 1-21,
N-terminal domain: residues 22-257,
C-terminal domain: residues 258-380.

Ross, et al.(2001) select PG0626 as one of fifteen vaccine candidate antigens, based on in vitro and in vivo laboratory studies of 120 candidates screened by bioinformatics techniques from the whole W50 genome.

TIGR ID: PG0694.

Proteomic Data: Proteomic Data Search
TIGR Annotationimmunoreactive 42 kDa antigen PG33, GroupT (unknown function)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG0694-1.220-0.290.04-0.940-0.290.04
LANLPG06260.430.820.521.10
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Previously sequenced from strain W50 and located in GenBank as AAD51068. Using gapped BLAST PG0626 is 29% similar to outer membrane protein OmpA in Vibrio cholerae (AAF95357,AAB86826).

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540489|ref|NP_904968.1|  immunoreactive 42 kDa antigen ...   740   0.0  
 gi|53828383|dbj|BAD54750.1|  hypothetical protein [Porphyrom...   731   0.0  
 gi|150007308|ref|YP_001302051.1|  outer membrane protein Omp...   353   8e-96
 gi|154490277|ref|ZP_02030538.1|  hypothetical protein PARMER...   345   2e-93
 gi|34540490|ref|NP_904969.1|  immunoreactive 43 kDa antigen ...   327   7e-88
 gi|53828382|dbj|BAD54749.1|  hypothetical protein [Porphyrom...   326   1e-87
 gi|5759277|gb|AAD51067.1|AF175714_1  immunoreactive 43kD ant...   326   2e-87
 gi|57918612|gb|AAW59418.1|  outer membrane protein HMP [Porp...   303   1e-80
 gi|53712590|ref|YP_098582.1|  putative outer membrane protei...   181   9e-44
 gi|60680803|ref|YP_210947.1|  putative outer membrane protei...   180   1e-43


InterPro Summary:  InterProScan

InterPro
IPR006664
Domain
Bacterial outer membrane protein
PR01021 [313-328]T 9.7E-5 PR01021 OMPADOMAIN[328-344]T 9.7E-5 PR01021 OMPADOMAIN OMPADOMAIN
InterPro
IPR006665
Domain
OmpA/MotB
PD000930 [282-339]T 0.0 PD000930 OmpA/MotB OmpA/MotB
PF00691 [282-369]T 1.8000014928719202E-11 PF00691 OmpA OmpA
PS51123 [270-380]T 0.0 PS51123 OMPA_2 OMPA_2
InterPro
IPR006690
Domain
OmpA-like
PS01068 [314-359]T 8.0E-5 PS01068 OMPA_1 OMPA_1
noIPR
unintegrated
unintegrated
G3DSA:3.30.1330.60 [265-378]T 1.50000306971087E-18 G3DSA:3.30.1330.60 G3DSA:3.30.1330.60 G3DSA:3.30.1330.60
PS51257 [1-15]F 0.0 PS51257 PROKAR_LIPOPROTEIN PROKAR_LIPOPROTEIN
SSF103088 [267-378]T 3.4E-20 SSF103088 SSF103088 SSF103088


COGS Summary:  COGS Search
BeTs to 6 clades of COG1360
COG name: Flagellar motor protein MotB
Functional Class: N
The phylogenetic pattern of COG1360 is -----Qv-EBrHUJ--OLinx
Number of proteins in this genome belonging to this COG is 5
Blocks Summary:  Blocks Search
***** IPB001145 (Bacterial outer membrane protein) with a combined E-value of 1.5e-09.
    IPB001145B    280-306
    IPB001145C    313-348


 ProDom Summary:  Protein Domain Search
Residues 310-364 are 54% similar to a (PROTEIN MEMBRANE OUTER PRECURSOR SIGNAL TRANSMEMBRANE) protein domain (PD000930 which is seen in O31154_VIBCH.


Paralogs:  Local Blast Search
Residues 1-376 share 46% identity with PG0627, an outer membrane protein, immediately upstream of PG0626.

Pfam Summary:  Pfam Search
Residues 282 to 369 (E-value = 1.6e-14) place PG0626 in the OmpA family which is described as OmpA family (PF00691)

 Structural Feature(s):
Feature Type  Start  Stop
cleavable signal  
1  
21
gram negative signal  
1  
21

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MKAKSLLLALAGLACTFSATAQEATTQNKAGMHTAFQRDKASDHWFIDIA
GGAGMALSGWNNDVDFVDRLSIVPTFGIGKWHEPYFGTRLQFTGFDIYGF
PQGSKERNHNYFGNAHLDFMFDLTNYFGVYRPNRVFHIIPWAGIGFGYKF
HSENANGEKVGSKDDMTGTVNVGLMLKFRLSRVVDFNIEGQAFAGKMNFI
GTKRGKADFPVMATAGLTFNLGKTEWTEIVPMDYALVNDLNNQINSLRGQ
VEELSRRPVSCPECPEPTQPTVTRVVVDNVVYFRINSAKIDRNQEINVYN
TAEYAKTNNAPIKVVGYADEKTGTAAYNMKLSERRAKAVAKMLEKYGVSA
DRITIEWKGSSEQIYEENAWNRIVVMTAAE

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAAGCTAAATCTTTATTATTAGCACTTGCGGGTCTCGCATGCACATT
CAGTGCAACAGCCCAAGAAGCTACTACACAGAACAAAGCAGGGATGCACA
CCGCATTCCAACGTGATAAGGCCTCCGATCATTGGTTCATTGACATTGCA
GGTGGAGCAGGTATGGCTCTCTCGGGATGGAATAATGATGTAGACTTTGT
AGATCGTCTAAGTATCGTTCCTACTTTCGGTATCGGTAAATGGCATGAGC
CTTATTTCGGTACTCGTCTCCAATTCACAGGATTCGACATCTATGGATTC
CCGCAAGGGAGCAAGGAGCGTAACCACAATTACTTTGGAAACGCCCACCT
TGACTTCATGTTCGATCTGACGAACTATTTCGGTGTATACCGTCCCAATC
GTGTCTTCCATATCATCCCATGGGCAGGTATAGGATTTGGTTATAAATTC
CATAGCGAAAACGCCAATGGTGAAAAAGTAGGAAGTAAAGATGATATGAC
CGGAACAGTTAATGTCGGTTTGATGCTGAAATTCCGCCTATCAAGAGTCG
TAGACTTCAATATTGAAGGACAAGCTTTTGCCGGAAAGATGAACTTTATC
GGGACAAAGAGAGGAAAAGCAGACTTCCCTGTAATGGCTACAGCAGGTCT
AACGTTCAACCTTGGCAAGACAGAGTGGACAGAAATTGTTCCTATGGACT
ATGCTTTGGTCAATGACCTGAACAACCAAATCAACTCACTTCGCGGTCAA
GTGGAAGAGTTGAGCCGTCGTCCTGTTTCATGCCCTGAATGCCCTGAGCC
TACACAGCCTACAGTTACTCGTGTAGTCGTTGACAATGTGGTTTACTTCC
GTATCAATAGTGCAAAGATTGATCGTAATCAAGAAATCAATGTTTACAAT
ACAGCTGAATATGCGAAGACCAACAACGCACCGATCAAGGTAGTAGGTTA
CGCTGACGAAAAAACCGGTACTGCGGCCTATAACATGAAGCTTTCAGAGC
GTCGTGCAAAAGCGGTAGCCAAGATGCTTGAAAAGTATGGTGTTTCTGCG
GATCGCATTACAATTGAATGGAAGGGCTCATCAGAGCAAATCTATGAAGA
GAACGCTTGGAATCGTATTGTAGTAATGACTGCAGCGGAA

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