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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0526 IGR0531 IGR0529 IGR0527 IGR0532 IGR0530 IGR0528 PG0624 PG0630 PG0628 omp40, - PG0626 omp41,oprF, - PG0627 cat2, - PG0623 abfD, - PG0625 glgP, - PG0629 PG0624 PG0630 PG0628 omp40, - PG0626 omp41,oprF, - PG0627 cat2, - PG0623 abfD, - PG0625 glgP, - PG0629 Type: tandem, Name: tan5 - 213 PG0624 PG0630 PG0628 omp40, - PG0626 omp41,oprF, - PG0627 cat2, - PG0623 abfD, - PG0625 glgP, - PG0629


LANL Gene ID: PG0627

GenBank Locus Tag: PG0695

DNA Molecule Name:
1  

GenBank ID:
34540490

Gene Name:
omp41  oprF  

Definition:
outer membrane protein

Cellular Location:
Outer membrane, Periplasm, Extracellular [Evidence]

Gene Start:
752158

Gene Stop:
750986

Gene Length:
1173

Molecular Weight*:
43412

pI*:
9.00

Net Charge*:
4.61

EC:
 

Functional Class:
Cell envelope  

Gene Ontology:
Cellular component
  GO:0009279    cell outer membrane
  GO:0016021    integral to membrane


Pathway: pathway table

Primary Evidence:
Veith,P.D.,Talbo,G.H.,Slakeski,N.,Reynolds,E.C., Identification of a novel heterodimeric outer membrane protein of Porphyromonas gingivalis by two-dimensional gel electrophoresis and peptide mass fingerprinting, Eur. J. Biochem. (2001) 268, 4748-4757, PMID: 11532011.

Ross BC, Czajkowski L, Hocking D, Margetts M, Webb E, Rothel L, Patterson M, Agius C, Camuglia S, Reynolds E, Littlejohn T, Gaeta B, Ng A, Kuczek ES, Mattick JS, Gearing D, Barr IG, Identification of vaccine candidate antigens from a genomic analysis of Porphyromonas gingivalis, Vaccine 2001 Jul 20;19(30):4135-42, PMID: 11457538.

Ross,B.C., Barr,I., Patterson,M., Agius,C., Rothel,L., Margetts,M., Hocking,D. and Webb,E, Porphyromonas gingivalis polypeptides and nucleic acids, Patent: Australia (AU 98/01023)-PCT 10-DEC-1998; CSL Ltd; 45 Poplar Road; R&D Parkville, Victoria; Australia.

Comment:
Veith, et al.(2001) have shown that in strain W50 omp40 (PG0626) and omp41 (PG0627) are co-transcribed, and they form heterodimers through disulfide bonds to each other. They find that with the possible exception of the proteinases, Omp40/41 are the most abundant proteins associated with the outer membrane of W50. Like OmpA, they are heat-modifiable and can be proteolytically cleaved to yield two domains, shown below. The N-terminus of each mature protein is pyroGlu. These authors suspect that omp40/41 are porins.

Veith et al. divide the chain thus:
Signal peptide: residues 1-20,
N-terminal domain: residues 21-268,
C-terminal domain: residues 269-391.

Ross, et al.(2001) select PG0627 as one of fifteen vaccine candidate antigens, based on in vitro and in vivo laboratory studies of 120 candidates screened by bioinformatics techniques from the whole W50 genome.

TIGR ID: PG0695

Proteomic Data: Proteomic Data Search
TIGR Annotationimmunoreactive 43 kDa antigen PG32, GroupT (unknown function)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG0695-1.400-0.910.00018-0.540-0.910.00018
LANLPG06270.380.530.690.67
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG0627 is essentially identical to strain W50 previously sequenced in GenBank as AAD51067. Using gapped BLAST residues 264-366 are 37% similar to outer membrane protein in Pseudomonas agarici (AAD24552) and residues 9-373 are 25% similar to an outer membrane protein in Pseudomonas aeruginosa (AAG05166).

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540490|ref|NP_904969.1|  immunoreactive 43 kDa antigen ...   793   0.0  
 gi|53828382|dbj|BAD54749.1|  hypothetical protein [Porphyrom...   793   0.0  
 gi|5759277|gb|AAD51067.1|AF175714_1  immunoreactive 43kD ant...   791   0.0  
 gi|57918612|gb|AAW59418.1|  outer membrane protein HMP [Porp...   362   3e-98
 gi|53828383|dbj|BAD54750.1|  hypothetical protein [Porphyrom...   312   3e-83
 gi|34540489|ref|NP_904968.1|  immunoreactive 42 kDa antigen ...   310   2e-82
 gi|154490277|ref|ZP_02030538.1|  hypothetical protein PARMER...   306   2e-81
 gi|150007308|ref|YP_001302051.1|  outer membrane protein Omp...   303   1e-80
 gi|60680803|ref|YP_210947.1|  putative outer membrane protei...   175   4e-42
 gi|53712590|ref|YP_098582.1|  putative outer membrane protei...   175   5e-42


InterPro Summary:  InterProScan

InterPro
IPR006664
Domain
Bacterial outer membrane protein
PR01021 [295-317]T 2.3E-10 PR01021 OMPADOMAIN[324-339]T 2.3E-10 PR01021 OMPADOMAIN[339-355]T 2.3E-10 PR01021 OMPADOMAIN OMPADOMAIN
InterPro
IPR006665
Domain
OmpA/MotB
PD000930 [293-350]T 0.0 PD000930 OmpA/MotB OmpA/MotB
PF00691 [294-384]T 3.09999945899369E-9 PF00691 OmpA OmpA
PS51123 [282-391]T 0.0 PS51123 OMPA_2 OMPA_2
InterPro
IPR006690
Domain
OmpA-like
PS01068 [326-371]T 8.0E-5 PS01068 OMPA_1 OMPA_1
noIPR
unintegrated
unintegrated
G3DSA:3.30.1330.60 [277-389]T 4.19999771339602E-17 G3DSA:3.30.1330.60 G3DSA:3.30.1330.60 G3DSA:3.30.1330.60
SSF103088 [280-389]T 2.4999999999999996E-20 SSF103088 SSF103088 SSF103088


COGS Summary:  COGS Search
BeTs to 5 clades of COG1360
COG name: Flagellar motor protein MotB
Functional Class: N
The phylogenetic pattern of COG1360 is -----Qv-EBrHUJ--OLinx
Number of proteins in this genome belonging to this COG is 5
Blocks Summary:  Blocks Search
***** IPB001145 (Bacterial outer membrane protein) with a combined E-value of 4.3e-13.
    IPB001145B    292-318
    IPB001145C    324-359


 ProDom Summary:  Protein Domain Search
Residues 285-373 are 36% similar to a (PROTEIN MEMBRANE OUTER PRECURSOR) protein domain (PD000930 which is seen in PORF_PSEAE.


Paralogs:  Local Blast Search
Residues 1-388 share 46% identity with PG0626, an outer membrane protein, immediately downstream of PG0627.

Pfam Summary:  Pfam Search
Residues 294 to 384 (E-value = 2.7e-12) place PG0627 in the OmpA family which is described as OmpA family (PF00691)

 Structural Feature(s):
Feature Type  Start  Stop
cleavable signal  
1  
20
gram negative signal  
1  
20

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MKVKYLMLTLVGAIALNASAQENTVPATGQLPAKNVAFARNKAGSNWFVT
LQGGVAAQFLNDNNNKDLMDRLGAIGSLSVGKYHSPFFATRLQINGGQAH
TFLGKNGEQEINTNFGAAHFDFMFDVVNYFAPYRENRFFHLIPWVGVGYQ
HKFIGSEWSKDNVESLTANVGVMMAFRLGKRVDFVIEAQAAHSNLNLSRA
YNAKKTPVFEDPAGRYYNGFQGMATAGLNFRLGAVGFNAIEPMDYALIND
LNGQINRLRSEVEELSKRPVSCPECPEVTPVTKTENILTEKAVLFRFDSH
VVDKDQLINLYDVAQFVKETNEPITVVGYADPTGNTQYNEKLSERRAKAV
VDVLTGKYGVPSELISVEWKGDSTQPFSKKAWNRVVIVRSK

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAGGTAAAGTACTTAATGCTCACATTGGTTGGAGCAATTGCACTGAA
CGCAAGTGCACAGGAGAATACTGTACCGGCAACGGGTCAGTTACCCGCTA
AGAATGTTGCTTTTGCTCGCAATAAAGCAGGCAGCAATTGGTTTGTAACA
CTGCAAGGCGGTGTTGCAGCGCAGTTCCTCAATGACAACAACAACAAAGA
CCTCATGGACCGCTTAGGAGCCATAGGTTCTCTTTCTGTCGGAAAGTATC
ACAGCCCTTTCTTTGCAACTCGTTTGCAAATTAACGGAGGTCAAGCCCAC
ACTTTCCTCGGAAAAAATGGCGAACAAGAAATCAACACCAATTTTGGTGC
AGCTCACTTCGACTTTATGTTTGATGTGGTTAACTACTTTGCACCATATC
GCGAAAATCGTTTCTTCCATTTAATTCCATGGGTAGGTGTTGGCTACCAA
CACAAATTCATCGGTAGCGAATGGAGCAAAGACAATGTGGAATCACTGAC
GGCGAATGTAGGAGTTATGATGGCTTTCAGATTAGGAAAGCGAGTAGACT
TTGTGATCGAAGCACAAGCAGCTCACTCCAATCTCAATCTAAGTCGCGCA
TACAATGCCAAGAAAACTCCCGTATTCGAAGATCCCGCAGGACGTTATTA
CAATGGATTCCAGGGGATGGCTACAGCAGGTCTTAATTTCCGCCTGGGAG
CCGTAGGCTTCAATGCCATTGAACCAATGGACTACGCACTTATCAATGAT
CTGAATGGTCAGATTAACCGTTTGCGCAGCGAGGTCGAAGAACTCTCAAA
ACGTCCTGTATCATGCCCCGAATGTCCTGAAGTAACTCCTGTTACTAAGA
CAGAAAATATACTGACGGAAAAAGCTGTACTGTTCCGTTTCGACAGCCAC
GTTGTGGACAAAGATCAATTGATCAACCTGTATGACGTAGCTCAGTTTGT
AAAAGAAACTAACGAGCCGATTACCGTTGTTGGTTATGCTGATCCTACGG
GTAATACTCAATACAACGAGAAATTGTCTGAGCGTCGGGCTAAAGCCGTT
GTTGATGTTCTGACAGGTAAATATGGTGTGCCTTCCGAATTAATCTCTGT
AGAATGGAAGGGCGACTCTACGCAACCGTTCAGCAAGAAAGCTTGGAATC
GTGTTGTAATCGTTCGCTCCAAG

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