LANL Gene ID: PG0718
GenBank Locus Tag: PG0798
DNA Molecule Name:
1
GenBank ID:
34540580
Gene Name:
ISPg3
Definition:
ISPg3 (IS195) transposase
Cellular Location:
Cytoplasm [Evidence]
Gene Start:
852986
Gene Stop:
853885
Gene Length:
900
Molecular Weight*:
35035
pI*:
10.30
Net Charge*:
20.26
EC:
Functional Class:
Transposon functions
Gene Ontology:
Biological process
GO:0006313 transposition, DNA-mediated
Molecular function
GO:0003677 DNA binding
GO:0004803 transposase activity
Pathway: pathway table
Primary Evidence:
Lewis JP, Macrina FL, IS195, an insertion sequence-like element associated with protease genes in Porphyromonas gingivalis, Infect Immun 1998 Jul;66(7):3035-42, PubMed: 9632563.
Secondary Evidence:
Kehrenberg C, Werckenthin C, Schwarz S, Tn5706, a transposon-like element from Pasteurella multocida mediating tetracycline resistance, Antimicrob Agents Chemother 1998 Aug;42(8):2116-8, PubMed: 9687418.
Comment:
TIGR ID: PG0798
Human Oral Microbiome Database:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
PG0718 is essentially identical to the P.gingivalis putative protease gb|AAC26596 previously sequenced in GenBank. Gapped BLAST shows numerous significant hits to transposases, including 52% similarity to a repeated transposase in Pasteurella multocida (emb|CAA75664, emb|CAA75661), 47% similarity to gb|AAG16999 in Piscirickettsia salmonis, and 27% simiarity to gb|AAF61311 in Clostridium cellulovorans.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
InterPro Summary: InterProScan
| InterPro IPR002559 Domain 
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| noIPR unintegrated |
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![[113-279]T 4.199997713396019E-32 PF01609 Transposase_11](http://www.ebi.ac.uk/InterProScan/images/H.gif){kind=small}
![[235-300]T 0.0052 SSF50249 Nucleic_acid_OB](http://www.ebi.ac.uk/InterProScan/images/B.gif){kind=small}
COGS Summary: COGS Search
No hit to the COGs database.
Blocks Summary: Blocks Search
No significant hit to the Blocks database.
ProDom Summary: Protein Domain Search
Residues 212-286 are 98% similar to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190030 which is seen in O87010_PORGI.
Residues 135-208 are identical to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in O87010_PORGI.
Residues 75-133 are identical to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190029 which is seen in O87010_PORGI.
Residues 1-74 are identical to a (PUTATIVE PROTEASE PROTEASE) protein domain (PD203593 which is seen in O87010_PORGI.
Residues 116-280 are 30% similar to a (HYPOTHETICAL 20.1 KD PROTEIN) protein domain (PD207068 which is seen in Q06444_BACST.
Paralogs: Local Blast Search
PG0718 is identical to PG0919, PG0236, PG0178 and PG1115, predicted ISPg3 transposases. PG0718 is similar to fragments of PG1663, PG1328, PG1660, PG0273.
Pfam Summary: Pfam Search
Residues 113 to 279 (E-value = 1.6e-35) place PG0718 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)
Structural Feature(s):
| Feature Type | Start | Stop |
| coil-coil |
Top PDB Hits:
-53% similar to PDB:1C04 IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI (E_value = );
-53% similar to PDB:1GIY CRYSTAL STRUCTURE OF THE RIBOSOME AT 5.5 A RESOLUTION. THIS FILE, 1GIY, CONTAINS THE 50S RIBOSOME SUBUNIT. THE 30S RIBOSOME SUBUNIT, THREE TRNA, AND MRNA MOLECULES ARE IN THE FILE 1GIX (E_value = );
-53% similar to PDB:1ML5 Structure of the E. coli ribosomal termination complex with release factor 2 (E_value = );
-53% similar to PDB:1RL2 RIBOSOMAL PROTEIN L2 RNA-BINDING DOMAIN FROM BACILLUS STEAROTHERMOPHILUS (E_value = );
-53% similar to PDB:1YL3 Crystal structure of 70S ribosome with thrS operator and tRNAs. Large subunit. The coordinates for the small subunit are in the pdb entry 1YL4. (E_value = );
Gene Protein Sequence:
MKTNIVDVFCIIDDFSKLFDETIKKKTLEEADKKRRNRKFKMSDSEVMTI
LILFHLSRYRDLKAFYLQYITHSCRSEFPHLVSYNRFVELQSRVGFKLIA
FLNMCCLGQCTGISFIDSTPLKACHIKRAHGHRTMRGWAQKGKSTMGWFY
GFKLHIVINDRGEIINYQITPGNCDDREPLKDGTFTKNLFGKLIADRGYI
SQNLFDRLFVDDIHMITKIKKNMKNSLMHLYDKVLLRKRALIETVNDMLK
NVCQIEHTRHRSANNFVTNLISGIIAYNILPKKPELNIEIIRNPNFPISA
Gene Nucleotide Sequence: Sequence Viewer
ATGAAGACAAATATAGTTGATGTTTTTTGCATCATAGATGATTTCTCCAA
GCTTTTTGATGAAACAATCAAGAAAAAGACCCTCGAAGAGGCAGACAAAA
AACGCAGGAATAGAAAGTTTAAGATGTCGGACAGTGAGGTCATGACCATC
CTGATCCTGTTTCATCTGTCAAGATACCGAGATTTGAAAGCTTTTTATCT
TCAATACATCACCCATTCTTGTCGATCCGAGTTTCCACATCTTGTCTCTT
ATAATCGCTTTGTGGAGCTGCAAAGCAGGGTAGGTTTCAAGCTGATAGCA
TTTCTCAATATGTGTTGTTTGGGTCAATGTACAGGCATCTCTTTCATCGA
TTCCACCCCACTGAAGGCTTGTCATATCAAACGAGCTCATGGGCATAGGA
CAATGAGGGGATGGGCTCAAAAAGGCAAAAGCACCATGGGTTGGTTTTAT
GGATTCAAGCTACATATTGTTATCAACGACAGGGGTGAAATCATCAACTA
TCAAATCACACCGGGCAATTGTGATGACAGAGAACCTCTGAAAGACGGAA
CATTCACCAAGAATCTTTTTGGCAAACTCATTGCCGATAGAGGCTACATT
TCCCAAAACCTTTTTGACCGGCTCTTTGTCGATGACATCCACATGATAAC
CAAAATCAAAAAGAACATGAAGAACTCCCTGATGCATCTATATGACAAAG
TTTTATTGAGAAAGAGAGCCCTGATCGAAACGGTCAATGATATGCTCAAA
AATGTCTGTCAGATAGAGCACACGAGACATCGCAGTGCCAACAATTTTGT
CACCAACCTGATCTCCGGTATCATCGCTTACAACATCCTGCCTAAAAAGC
CTGAACTCAATATTGAAATCATCAGAAACCCTAACTTTCCTATTTCCGCT