Basic Search | Intermediate Search | Advanced Search | Gene Image Map |  Sequence Search |  Protein Motif Search |  Repeat Search |  DNA/RNA Motif Search |  Home

Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0624 IGR0623 IGR0615 IGR0621 IGR0618 IGR0620 IGR0622 IGR0617 IGR0619 IGR0616 ISPg1, - PG0730 PG0733 PG0734 ISPg1, - PG0731 ruvA, - PG0729 ISPg1, - PG0732 sov, - PG0727 PG0734.1 PG0737 PG0736 PG0735 ISPg1, - PG0730 PG0733 PG0734 ISPg1, - PG0731 ruvA, - PG0729 ISPg1, - PG0732 sov, - PG0727 PG0734.1 PG0737 PG0736 PG0735 Type: IS element, Name: ISPg1 fragment - 3 ISPg1, - PG0730 PG0734 ISPg1, - PG0731 ruvA, - PG0729 ISPg1, - PG0732 sov, - PG0727 sov, - PG0728 sov, - PG0728 PG0733 PG0734.1 PG0736 PG0735 PG0737


LANL Gene ID: PG0732

GenBank Locus Tag: PG0813

DNA Molecule Name:
1  

Genomic Island ID:
7

GenBank ID:


 Gene Name:
ISPg1  

 Definition:
ISPg1 fragment (IS1106-related transposase fragment)

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
875589

Gene Stop:
874843

Gene Length:
747

Molecular Weight*:
28945

pI*:
8.60

Net Charge*:
5.70

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


 Pathway: pathway table

 Primary Evidence:
Hanley,S.A., Aduse-Opoku,J. and Curtis,M.A.
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis
W50 has arisen via horizontal gene transfer
Infect. Immun. 67 (3), 1157-1171 (1999)

Maley,J. and Roberts,I.S.
Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements
Microbiol. Lett. 123 (1-2), 219-224 (1994)

Shibata,Y., Hayakawa,M., Takiguchi,H., Shiroza,T. and Abiko,Y.
Determination and characterization of the hemagglutinin-associated
short motifs found in Porphyromonas gingivalis multiple gene
products. J. Biol. Chem. 274 (8), 5012-5020 (1999)

 Comment:
Member of the IS5 family of elements. Corresponds to the N-terminal half of full-length elements such as PG1662.

TIGR ID: PG0813

 Proteomic Data: Proteomic Data Search
TIGR AnnotationISPg1, transposase, truncation, GroupG (disrupted reading frame) ,GroupL (other categories)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG08131.040.000591.851.4e-061.415.5e-081.851.4e-06
LANLPG07322.063.622.663.75
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Several hits in gapped BLAST to previously sequenced IS1126-related transposases from P.gingivalis. Residues 31-219 are 31% similar to an IS1168 sequence from Bacteroides vulgatus (X71444).

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|4001716|dbj|BAA35090.1|  ORF7 [Porphyromonas gingivalis]       446   e-124
 gi|34540279|ref|NP_904758.1|  ISPg1, transposase [Porphyromo...   442   e-123
 gi|34540041|ref|NP_904520.1|  ISPg1, transposase [Porphyromo...   430   e-119
 gi|3901097|emb|CAA10225.1|  transposase [Porphyromonas gingi...   424   e-117
 gi|3869078|dbj|BAA34342.1|  Insertion sequence IS1126-like g...   309   8e-83
 gi|1361314|pir||S57895  hypothetical protein 1 - Porphyromon...   308   2e-82
 gi|34541013|ref|NP_905492.1|  ISPg1, transposase, internal d...   307   3e-82
 gi|91218509|ref|ZP_01255448.1|  ISPg1, transposase [Psychrof...   188   2e-46
 gi|91216745|ref|ZP_01253710.1|  ISPg1, transposase [Psychrof...   186   1e-45
 gi|67939722|ref|ZP_00532213.1|  Transposase, IS4 [Chlorobium...   116   1e-24


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [147-220]T 4.3e-10 PF01609 Transposase_11 Transposase_11


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 157-230 are 95% similar to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in Q9ZAD0_PORGI.

Residues 12-109 are 97% similar to a (TRANSPOSASE PROTEIN INSERTION ELEMENT) protein domain (PD007264 which is seen in Q9ZNB4_PORGI.

Residues 110-156 are 97% similar to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187846 which is seen in Q9ZA61_PORGI.


Paralogs:  Local Blast Search
PG0732 is partly similar to PG1919, PG1230, PG0686, PG1798, PG0756, PG1755, PG1662, PG1422, PG1267, PG1184, PG1087, PG1062, PG1050, PG0918, PG0763, PG0740, PG0169, PG0839, PG1099, PG0499, PG0420, PG1665, PG0689, PG1215, PG1895, PG0044, PG0883, PG0842, PG1608, PG1159, PG1231, PG0884, PG0731 and PG0730, all predicted ISPg1 transposases. Specifically residues 1-230 are nearly identical to the N-termianl half of full-length ISPg1 elements such as PG1662.

Pfam Summary:  Pfam Search
No significant hits to the Pfam 11.0 database

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
57  
73
non-globular  
168  
214

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MAYQSKNTDEHVTFADALLSKRYRKAQNDFLNQVDTLIDWRPIRTLINKK
YTKRQNAIGAPAYDVILLFKMLLPKTWYNLSDCALEERINDSITFSRFLG
LKMEEVSPDHSAISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLV
DASLVETPHKPNGSITIEVADDRQDNRSEAEKEAEEDYQKQVVRQRKGTD
EEARWVYKQKRYHYGYKKHCPANVQGIVQKETIARHFCDIFACIACVYQ

Gene Nucleotide Sequence:  Sequence Viewer
ATGGCATACCAATCCAAGAATACCGATGAGCATGTAACATTTGCAGACGC
ACTCCTTTCAAAGCGTTATCGCAAAGCACAAAACGACTTCCTCAATCAGG
TTGACACGCTTATCGATTGGCGTCCGATCAGGACGCTGATCAACAAGAAA
TACACGAAGCGACAAAATGCCATCGGCGCCCCGGCTTATGACGTGATTCT
CTTATTCAAGATGTTGCTTCCGAAGACATGGTACAACCTCAGTGATTGTG
CTTTGGAGGAGCGCATCAATGATTCAATCACCTTTTCCCGATTCTTGGGG
CTGAAGATGGAAGAGGTATCTCCCGACCACAGCGCCATCAGTCGATTTCG
TTCGGCACTGACAGAGTTGGGTCTCATGGACAAACTATTGGCGCAGTTTA
ACAAACAACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTC
GATGCAAGCCTTGTGGAGACGCCACACAAACCCAACGGAAGCATTACGAT
TGAAGTCGCAGACGACAGGCAAGACAATCGGAGCGAGGCGGAAAAAGAGG
CAGAGGAGGATTATCAAAAACAGGTTGTCCGTCAGCGTAAAGGGACGGAT
GAAGAAGCCCGTTGGGTTTACAAACAAAAGCGTTATCACTACGGATACAA
AAAGCATTGTCCGGCCAATGTTCAAGGCATTGTTCAAAAGGAGACCATTG
CACGGCATTTTTGCGACATATTTGCCTGCATTGCTTGCGTATATCAA

Los Alamos National Laboratory • Est 1943

Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration
Inside | © Copyright 2006 LANS LLC All rights reserved | Disclaimer/Privacy