LANL Gene ID: PG0839
GenBank Locus Tag: PG0939
DNA Molecule Name:
1
GenBank ID:
Gene Name:
ISPg1
Definition:
ISPg1 transposase fragment (IS1106-related transposase)
Cellular Location:
Cytoplasm [Evidence]
Gene Start:
1001281
Gene Stop:
1000505
Gene Length:
777
Molecular Weight*:
29537
pI*:
9.80
Net Charge*:
8.52
EC:
Functional Class:
Transposon functions
Gene Ontology:
Biological process
GO:0006313 transposition, DNA-mediated
Molecular function
GO:0003677 DNA binding
GO:0004803 transposase activity
Pathway: pathway table
Primary Evidence:
Hanley,S.A., Aduse-Opoku,J. and Curtis,M.A.
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis
W50 has arisen via horizontal gene transfer
Infect. Immun. 67 (3), 1157-1171 (1999)
Maley,J. and Roberts,I.S.
Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements
Microbiol. Lett. 123 (1-2), 219-224 (1994)
Shibata,Y., Hayakawa,M., Takiguchi,H., Shiroza,T. and Abiko,Y.
Determination and characterization of the hemagglutinin-associated
short motifs found in Porphyromonas gingivalis multiple gene
products. J. Biol. Chem. 274 (8), 5012-5020 (1999)
Secondary Evidence:
Knight,A.I., Ni,H., Cartwright,K.A. and McFadden,J.J.
Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidis.
Mol. Microbiol. 6 (11), 1565-1573 (1992)
Comment:
Member of the IS5 family of elements. Reproduces the middle and C-terminal domains of full-length ISPg1 elements (e.g. PG1662).
TIGR ID: PG0939
Human Oral Microbiome Database:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
PG0839 is equivalent to the previously sequenced AJ130872 and AB015879 in GenBANK. Residues 31-350 are 34% similar to the IS1106 sequence of N.meningitidis (AE002488).
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
InterPro Summary: InterProScan
| InterPro IPR002559 Domain 
|
| |||||||
![[45-246]T 4.7e-36 PF01609 Transposase_11](http://www.ebi.ac.uk/InterProScan/images/H.gif){kind=small}
COGS Summary: COGS Search
No hit to the COGs database.
Blocks Summary: Blocks Search
No significant hit to the Blocks database.
ProDom Summary: Protein Domain Search
Residues 201-259 are 98% similar to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187845 which is seen in Q9ZAD0_PORGI.
Residues 55-200 are 85% similar to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in Q9ZAD0_PORGI.
Residues 8-54 are identical to a (TRANSPOSASE ORF7 INSERTION SEQUENCE) protein domain (PD187846 which is seen in Q9ZA61_PORGI.
Paralogs: Local Blast Search
PG0839 is is virtually identical to PG1087, PG0763, PG1755, PG0686, PG0756, PG1798, PG1230, PG1919, PG0044, PG0689, PG1215, PG1665, PG184, PG1895, all ISPg1 fragments that reproduce the middle and C-terminal residues of PG1267, PG0169, PG1050, PG1062, PG0740, PG0918, PG1662, PG1422, PG0420, PG0499, PG1099.
Also, partial similarities to PG0732, PG0842, PG0883, PG1231, PG0730, PG1159, PG1608.
Pfam Summary: Pfam Search
Residues 45 to 246 (E-value = 3.7e-36) place PG0839 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular |
Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).
Gene Protein Sequence:
MEEVSPDHSTISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLVDA
SLVETPHKPNGSITIEVADDREDNRSEEEKEAEEDYQKQVVRQRKGTDEE
ARWVYKQKRYHYGYKKHCLTNVQGIVQKVITTAANRSDTKEFIPLLQGAN
IPQGTAVFADKGYACGENRSYLQTHHLQDGIMHKAQRNRALTEEEKQGNK
AIGPIRSTIERTFGSIRRWFHGGRCRYRGLAKTHTQNILESIAFNLYRTP
GIIMSSSLG
Gene Nucleotide Sequence: Sequence Viewer
ATGGAAGAGGTATCTCCCGACCACAGCACCATCAGTCGATTTCGTTCGGC
ACTGACAGAGTTGGGGCTCATGGACAAACTATTGGCGCAGTTTAACAAAC
AACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTCGATGCA
AGCCTTGTGGAGACGCCGCATAAACCCAACGGAAGCATTACGATTGAAGT
CGCAGACGACAGAGAAGACAATCGGAGCGAGGAGGAAAAAGAGGCAGAGG
AGGATTATCAAAAACAGGTTGTCCGTCAGCGTAAAGGGACGGATGAAGAA
GCCCGTTGGGTTTACAAACAAAAGCGTTATCACTACGGATACAAAAAGCA
TTGTCTGACCAATGTTCAAGGCATTGTTCAAAAGGTGATAACGACAGCAG
CGAATCGCAGTGACACGAAGGAGTTTATTCCGCTATTGCAGGGTGCAAAC
ATACCTCAAGGCACAGCCGTCTTCGCGGACAAAGGATATGCTTGCGGGGA
AAATCGTTCCTACCTGCAAACCCATCACCTTCAAGACGGCATCATGCACA
AGGCACAACGCAACAGGGCATTGACCGAGGAAGAGAAGCAAGGAAACAAA
GCAATCGGTCCGATACGGAGCACCATCGAACGCACCTTTGGCAGTATTCG
CCGGTGGTTTCATGGCGGACGATGTCGATACCGGGGACTTGCCAAGACCC
ATACTCAAAACATTCTTGAAAGCATCGCCTTTAATTTATACAGGACCCCG
GGGATAATTATGTCCTCATCTCTAGGA