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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0721 IGR0720 IGR0715 IGR0717 IGR0722 IGR0719 IGR0718 IGR0716 PG0845 ISPg1, - PG0842 ISPg1, - PG0839 natA, - PG0844 natB, - PG0843 PG0838 ISPg5, - PG0840 amn, - PG0846 ISPg5, - PG0841 PG0845 ISPg1, - PG0842 ISPg1, - PG0839 natA, - PG0844 natB, - PG0843 PG0838 ISPg5, - PG0840 amn, - PG0846 ISPg5, - PG0841 Type: tandem, Name: tan7 - 215 Type: IS element, Name: ISPg1 - 2 Type: IS element, Name: ISPg1 fragment - 3 Type: IS element, Name: ISPg5 - 8 Type: tandem, Name: tan8 - 216 PG0845 ISPg1, - PG0842 ISPg1, - PG0839 natA, - PG0844 natB, - PG0843 PG0838 ISPg5, - PG0840 amn, - PG0846 ISPg5, - PG0841


LANL Gene ID: PG0841

GenBank Locus Tag: PG0943

DNA Molecule Name:
1  

GenBank ID:
34540696

Gene Name:
ISPg5  

Definition:
ISPg5 transposase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1002654

Gene Stop:
1003589

Gene Length:
936

Molecular Weight*:
36207

pI*:
10.00

Net Charge*:
15.47

EC:
 

Functional Class:
Transposon functions  

Gene Ontology:
Biological process
  GO:0015074    DNA integration

Molecular function
  GO:0003677    DNA binding


Pathway: pathway table

Primary Evidence:
Califano,J.V., Kitten,T., Lewis,J.P., Macrina,F.L., Fleischmann,R.D., Fraser,C.M.,Duncan,M.J. and Dewhirst,F.E. Characterization of Porphyromonas gingivalis insertion
sequence-like element ISPg5.
Infect. Immun. 68 (9), 5247-5253 (2000)

Comment:
One of eleven ISPg5 elements in the genome (see Paralogs). Califano et al. report considerable strain diversity of these (Infect. Immun. 68 (9), 5247-5253 (2000)).

Member of the IS3 family of insertion elements. See PG0840 for associated ORF.

TIGR ID: PG0943

Human Oral Microbiome Database:
View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
PG0841 is equivalent to the previously reported gb|AAF69128.1| in GenBANK.

Numerous significant hits in gapped BLAST to transposase proteins; e.g. residues 91-213 are 56% similar to transposase homolog (U67062) of Bacteriodes thetaiotaomicrom, residues 64-287 are 32% similar to InsB (AF153317) of Shigella dysenteriae, residues 64-268 are 33% similar to Hp1 (AF081284) of E.coli.

Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540696|ref|NP_905175.1|  ISPg5, transposase Orf2 [Porph...   627   e-178
 gi|118200270|dbj|BAF37077.1|  transposase Orf2 [Porphyromona...   622   e-176
 gi|34540399|ref|NP_904878.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34541641|ref|NP_906120.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34539915|ref|NP_904394.1|  ISPg5 transposase Orf2 [Porphy...   617   e-175
 gi|34540277|ref|NP_904756.1|  ISPg5, transposase Orf2 [Porph...   615   e-174
 gi|34541341|ref|NP_905820.1|  ISPg5, transposase Orf2 [Porph...   610   e-173
 gi|34540248|ref|NP_904727.1|  ISPg5, transposase Orf2 [Porph...   609   e-173
 gi|34541287|ref|NP_905766.1|  ISPg5, transposase Orf2 [Porph...   607   e-172
 gi|34539888|ref|NP_904367.1|  ISPg5 transposase Orf2 [Porphy...   606   e-172


InterPro Summary:  InterProScan

InterPro
IPR001584
Domain
Integrase, catalytic core
PF00665 [110-273]T 6.5e-32 PF00665 rve rve
PS50994 [110-276]T 18.646 PS50994 INTEGRASE INTEGRASE
noIPR
unintegrated
unintegrated
G3DSA:3.30.420.10 [113-281]T 7.5e-07 G3DSA:3.30.420.10 no description no description


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01836 (Transposase) with a combined E-value of 4.3e-23.
    PF01836B    103-154
    PF01836C    181-190
    PF01836D    215-222
    PF01836E    253-272


 ProDom Summary:  Protein Domain Search
No significant hit to the ProDom database.

Paralogs:  Local Blast Search
PG0841 is paralogously related to PG1861, PG1491, PG0391, PG0418, PG1436, PG1796, PG0034, PG0533, PG1247 and PG0007.

Pfam Summary:  Pfam Search
Residues 110 to 273 (E-value = 5.7e-32) place PG0841 in the rve family which is described as Integrase core domain (PF00665)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
LLGFSRQAFYKRHLNDLARHEEDVLCSSIIQYCWHLRQAEHLPQAGFREL
MVLCQQYFGPKFTLGRDRFCALLRRHGMMLRKRSVRPQTTNSRHRLYKYE
DLLNTEPKFVPQRPGELLVADITYVAYQDGFAYLSLLTDAYSRCIVGYCL
HPTLEVEGCLNALHQAFAFYDQHQIDTSNMIHHSDRGIQYASKSYTDLLH
GRGCRISMTQTGDPLHNALAERMNNTLKNSWHISSSKQSFDQALLSVDRA
VRMYNEARPHQALGAKTPMQVIAPESKNPLLTRREHGPEIAPELYRRMNV
RQRANFARVNRN

Gene Nucleotide Sequence:  Sequence Viewer
CTGCTTGGCTTTAGCCGTCAGGCCTTTTATAAACGTCATCTCAACGATTT
AGCTAGGCACGAGGAGGATGTCCTATGCAGTAGTATCATCCAATATTGTT
GGCACTTAAGACAAGCAGAGCACCTACCTCAAGCTGGCTTCCGAGAGCTC
ATGGTGCTTTGTCAGCAATACTTCGGCCCCAAGTTCACGCTTGGGCGCGA
TCGCTTTTGCGCCCTACTCAGACGCCATGGTATGATGCTGCGCAAACGGT
CTGTGCGCCCTCAGACAACCAACTCTCGGCATAGGCTCTACAAGTATGAA
GACCTGCTCAATACAGAGCCTAAGTTTGTGCCTCAAAGACCTGGGGAACT
CCTTGTAGCAGACATCACCTACGTGGCTTACCAAGATGGGTTTGCCTATC
TCTCCCTACTGACCGATGCCTATAGTCGCTGTATCGTAGGGTATTGCTTA
CATCCTACCCTAGAGGTTGAAGGCTGTTTGAATGCTCTACATCAAGCCTT
TGCTTTCTACGATCAGCATCAAATTGATACGAGCAATATGATTCATCATA
GCGACCGAGGCATTCAGTATGCCAGTAAGAGCTACACCGATCTGTTGCAT
GGGCGAGGCTGTCGCATCAGCATGACTCAGACGGGAGATCCTCTGCATAA
TGCATTGGCTGAGCGGATGAACAATACGCTCAAGAACTCGTGGCACATCT
CCTCTTCGAAGCAGTCTTTTGATCAAGCACTCCTTTCTGTAGACCGAGCC
GTGCGCATGTACAACGAGGCGCGTCCACATCAAGCTCTAGGGGCGAAAAC
GCCTATGCAAGTCATTGCGCCAGAGTCTAAAAATCCGTTACTAACGAGGA
GGGAGCATGGGCCAGAGATTGCCCCCGAGCTATACCGAAGGATGAATGTC
AGACAAAGAGCTAACTTTGCTCGTGTCAACCGAAAT

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