LANL Gene ID: PG0883
GenBank Locus Tag: PG0988
DNA Molecule Name:
1
GenBank ID:
Gene Name:
ISPg1
Definition:
ISPg1 fragment (IS1106-related fragment)
Cellular Location:
Cytoplasm [Evidence]
Gene Start:
1049670
Gene Stop:
1049248
Gene Length:
423
Molecular Weight*:
15667
pI*:
10.70
Net Charge*:
13.23
EC:
Functional Class:
Transposon functions
Gene Ontology:
Biological process
GO:0006313 transposition, DNA-mediated
Molecular function
GO:0003677 DNA binding
GO:0004803 transposase activity
Pathway: pathway table
Primary Evidence:
Hanley,S.A., Aduse-Opoku,J. and Curtis,M.A.
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis
W50 has arisen via horizontal gene transfer
Infect. Immun. 67 (3), 1157-1171 (1999)
Maley,J. and Roberts,I.S.
Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements
Microbiol. Lett. 123 (1-2), 219-224 (1994)
Shibata,Y., Hayakawa,M., Takiguchi,H., Shiroza,T. and Abiko,Y.
Determination and characterization of the hemagglutinin-associated
short motifs found in Porphyromonas gingivalis multiple gene
products. J. Biol. Chem. 274 (8), 5012-5020 (1999)
Secondary Evidence:
Knight,A.I., Ni,H., Cartwright,K.A. and McFadden,J.J.
Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidis.
Mol. Microbiol. 6 (11), 1565-1573 (1992)
Comment:
Member of the IS5 family of elements. PG0883 and PG0884 could together constitute a full-length ISPg1 element such as PG1662.
TIGR ID: PG0988
Human Oral Microbiome Database:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
PG0883 is equivalent to the previously sequenced AJ130872 and AB015879 in GenBANK. Residues 2-128 are 42% similar to the IS1106 sequence of N.meningitidis (AJ001313).
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
InterPro Summary: InterProScan
| InterPro IPR002559 Domain 
|
| |||||||
![[1-128]T 7e-08 PF01609 Transposase_11](http://www.ebi.ac.uk/InterProScan/images/H.gif){kind=small}
COGS Summary: COGS Search
No hit to the COGs database.
Blocks Summary: Blocks Search
No significant hit to the Blocks database.
ProDom Summary: Protein Domain Search
Residues 84-136 are 73% similar to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187845 which is seen in Q9ZA61_PORGI.
Residues 1-82 are 46% similar to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in Q9ZAD0_PORGI.
Paralogs: Local Blast Search
PG0883 is similar to the C-terminal regions of PG1231, PG1798, PG1662, PG1422, PG1267, PG1184, PG1087, PG1062, PG1050, PG0918, PG0763, PG0756, PG0740, PG0169, PG1919, PG1230, PG0686, PG0044, PG1755, PG1099, PG0499, PG0420, PG0689, PG1215, PG0839, PG1665, PG1895, PG0730, PG1608, PG1159, all ISPg1-related elements.
Pfam Summary: Pfam Search
Residues 1 to 128 (E-value = 7.2e-08) place PG0883 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)
Structural Feature(s):
| Feature Type | Start | Stop |
| No predicted structural features. |
Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).
Gene Protein Sequence:
LTDEQGLVETVVTTSANCADTVVLPELIKKSKLPGGISVLADKGYCSKKN
SEYLARQGLIDGIMLKAHRGQVLSESQKQLNRMISKTRCLIERTFGSIRR
WFCGGRCRYRGLAKTHTRNILEAMAYNLKRMPGLLVLQGAK
Gene Nucleotide Sequence: Sequence Viewer
TTGACGGACGAGCAGGGTTTGGTAGAAACAGTGGTCACGACCTCGGCTAA
TTGTGCAGACACGGTTGTATTACCTGAGCTGATCAAGAAGTCCAAGTTGC
CGGGAGGAATTAGTGTTTTGGCCGACAAAGGCTATTGCAGCAAGAAGAAT
TCGGAGTATTTGGCCCGTCAAGGTTTGATAGATGGAATTATGCTCAAGGC
TCATCGAGGTCAAGTCCTCAGTGAAAGTCAGAAACAACTCAATCGGATGA
TCAGTAAGACACGCTGCTTGATAGAACGGACCTTCGGTAGTATTCGACGA
TGGTTCTGTGGCGGACGATGTCGCTACCGAGGGTTAGCCAAGACACATAC
CCGGAATATCCTTGAAGCTATGGCCTACAATCTCAAGCGTATGCCGGGGC
TTCTTGTGCTTCAAGGCGCAAAA