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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR0785 IGR0786 IGR0784 IGR0783 IGR0782 IGR0781 ISPg1, - PG0918 PG0920 ISPg3, - PG0919 PG0917 PG0922 PG0916 uvrA, - PG0923 ISPg1, - PG0918 PG0920 ISPg3, - PG0919 PG0917 PG0922 PG0916 uvrA, - PG0923 Type: IS element, Name: ISPg1 - 2 Type: IS element, Name: ISPg3 fragment - 6 Type: IS element, Name: ISPg3 fragment - 6 ISPg1, - PG0918 PG0920 ISPg3, - PG0919 PG0917 PG0922 PG0916 uvrA, - PG0923 PG0921 PG0921


LANL Gene ID: PG0919

GenBank Locus Tag: PG1032

DNA Molecule Name:
1  

GenBank ID:
34540773

Gene Name:
ISPg3  

Definition:
ISPg3 (IS195) transposase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1094475

Gene Stop:
1095374

Gene Length:
900

Molecular Weight*:
35076

pI*:
10.40

Net Charge*:
22.02

EC:
 

Functional Class:
Transposon functions  

Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


Pathway: pathway table

Primary Evidence:
Lewis JP, Macrina FL, IS195, an insertion sequence-like element associated with protease genes in Porphyromonas gingivalis. Infect Immun 1998 Jul;66(7):3035-42, PubMed: 9632563.

Secondary Evidence:
Kehrenberg C, Werckenthin C, Schwarz S, Tn5706, a transposon-like element from Pasteurella multocida mediating tetracycline resistance. Antimicrob Agents Chemother 1998 Aug;42(8):2116-8, PubMed: 9687418.

Comment:
TIGR ID: PG1032

Human Oral Microbiome Database:
View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
PG0919 is equivalent to the previously sequenced gb|AAC26596.1, a predicted IS195 transposase. Residues 75-286 are 52% similar to a transposase sequence from Pasteurella multocida (Y15510).



Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540773|ref|NP_905252.1|  ISPg3, transposase [Porphyromo...   604   e-171
 gi|3323385|gb|AAC26596.1|  putative protease [Porphyromonas ...   597   e-169
 gi|34540580|ref|NP_905059.1|  ISPg3, transposase [Porphyromo...   596   e-169
 gi|34540105|ref|NP_904584.1|  ISPg3, transposase [Porphyromo...   596   e-169
 gi|34540050|ref|NP_904529.1|  ISPg3, transposase [Porphyromo...   595   e-168
 gi|61652425|gb|AAX47722.1|  transposase [Porphyromonas gingi...   595   e-168
 gi|34540967|ref|NP_905446.1|  ISPg3, transposase [Porphyromo...   591   e-167
 gi|150009840|ref|YP_001304583.1|  putative transposase [Para...   306   7e-82
 gi|150002628|ref|YP_001297372.1|  putative transposase [Bact...   295   3e-78
 gi|89142213|ref|YP_512234.1|  transposase [Pasteurella treha...   281   3e-74


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [113-279]T 1.3999989204989E-32 PF01609 Transposase_11 Transposase_11
InterPro
IPR012337
Domain
Polynucleotidyl transferase, Ribonuclease H fold
SSF53098 [193-280]T 0.014 SSF53098 RNaseH_fold RNaseH_fold


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 212-286 are 96% similar to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190030 which is seen in O87010_PORGI.

Residues 135-208 are identical to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in O87010_PORGI.

Residues 75-133 are 98% similar to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190029 which is seen in O87010_PORGI.

Residues 1-74 are 98% similar to a (PUTATIVE PROTEASE PROTEASE) protein domain (PD203593 which is seen in O87010_PORGI.

Residues 116-280 are 30% similar to a (HYPOTHETICAL 20.1 KD PROTEIN) protein domain (PD207068 which is seen in Q06444_BACST.


Paralogs:  Local Blast Search
PG0919 is identical to PG0718, PG0236, PG0178 and PG1115, predicted ISPg3 transposases. PG0919 is similar to fragments of PG1663, PG1328, PG1660, PG0273.

Pfam Summary:  Pfam Search
Residues 113 to 279 (E-value = 5.3e-36) place PG0919 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)

 Structural Feature(s):
Feature Type  Start  Stop
coil-coil  
220  
255

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MKTNIVDVFCIIDDFSKLFDEAIKKKTLEEADKKRRNRKFKMSDSEVMTI
LILFHLSRYRDLKAFYLQYITHSCRSEFPHLVSYNRFVELQSRVGFKLIA
FLNMCCLGQCTGISFIDSTPLKACHIKRARGHRTMRGWAQKGKSTMGWFY
GFKLHIVINDRGEIINYQITPGNCDDREPLKDGTFTKNLFGKLIADRGYI
SQNLFDRLFVDDIHMITKIKKNMKNSLMHLYDKVLLRKRALIETVNDILK
NLCRIEHTRHRSVNNFVTNLISGIIAYNILPKKPELNIEIIRNPNFPISA


Gene Nucleotide Sequence:  Sequence Viewer
ATGAAGACAAATATAGTTGATGTTTTTTGCATCATAGATGATTTCTCCAA
GCTTTTTGATGAAGCAATCAAGAAAAAGACCCTCGAAGAGGCAGACAAAA
AACGCAGGAATAGAAAGTTTAAGATGTCGGACAGTGAGGTCATGACCATC
CTGATCCTGTTTCATCTGTCAAGATACCGAGATTTGAAAGCTTTTTATCT
TCAATACATTACCCATTCTTGTCGATCCGAGTTTCCACATCTTGTCTCTT
ATAATCGCTTTGTGGAGCTGCAAAGCAGGGTAGGTTTCAAGCTGATAGCA
TTTCTCAATATGTGTTGTTTGGGTCAATGTACAGGCATCTCTTTCATCGA
TTCCACCCCACTGAAGGCTTGTCATATCAAACGAGCTCGTGGGCATAGGA
CAATGAGGGGATGGGCTCAAAAAGGCAAAAGCACCATGGGTTGGTTTTAT
GGATTCAAGCTACATATTGTTATCAACGACAGGGGTGAAATTATCAACTA
TCAAATCACACCGGGCAATTGTGATGACAGAGAACCTCTGAAAGACGGAA
CATTCACCAAGAATCTTTTTGGCAAACTCATTGCCGATAGAGGCTACATT
TCCCAAAACCTTTTTGACCGGCTCTTTGTCGATGACATCCACATGATAAC
CAAAATCAAAAAGAACATGAAGAACTCCCTGATGCATCTATATGACAAAG
TTTTATTGAGAAAGAGAGCCTTGATCGAAACGGTCAATGATATACTCAAA
AATCTCTGCCGGATAGAGCACACGAGACATCGCAGTGTCAACAATTTTGT
CACCAACCTGATCTCCGGTATCATCGCTTACAACATCCTGCCTAAAAAGC
CTGAACTCAATATTGAAATCATCAGAAACCCTAACTTTCCTATTTCCGCT

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