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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap tRNA-Ile-2 tRNA-Ala-3 rRNA-5S-2 rRNA-23S-2 IGR0953 IGR0955 IGR0954 IGR0956 nrdG, - PG1112 ISPg3, - PG1115 nrdD, - PG1113 ISPg4,tnp, - PG1114 nrdG, - PG1112 ISPg3, - PG1115 nrdD, - PG1113 ISPg4,tnp, - PG1114 Type: IS element, Name: ISPg3 - 5 Type: IS element, Name: ISPg4 - 7 nrdG, - PG1112 ISPg3, - PG1115 nrdD, - PG1113 ISPg4,tnp, - PG1114


LANL Gene ID: PG1115

GenBank Locus Tag: PG1262

DNA Molecule Name:
1  

Genomic Island ID:
23

GenBank ID:
34540967

 Gene Name:
ISPg3  

 Definition:
ISPg3 (IS195) transposase

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
1340727

Gene Stop:
1339828

Gene Length:
900

Molecular Weight*:
34930

pI*:
10.30

Net Charge*:
20.22

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


 Pathway: pathway table

 Primary Evidence:
Lewis JP, Macrina FL, IS195, an insertion sequence-like element associated with protease genes in Porphyromonas gingivalis. Infect Immun 1998 Jul;66(7):3035-42, PubMed: 9632563.

 Secondary Evidence:
Kehrenberg C, Werckenthin C, Schwarz S, Tn5706, a transposon-like element from Pasteurella multocida mediating tetracycline resistance. Antimicrob Agents Chemother 1998 Aug;42(8):2116-8, PubMed: 9687418.

 Comment:
TIGR ID: PG1262

 Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG1115 is equivalent to the previously sequenced gb|AAC26596.1, a predicted IS195 transposase. Residues 75-286 are 52% similar to a transposase sequence from Pasteurella multocida (Y15510).

Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540967|ref|NP_905446.1|  ISPg3, transposase [Porphyromo...   610   e-173
 gi|61652425|gb|AAX47722.1|  transposase [Porphyromonas gingi...   593   e-168
 gi|3323385|gb|AAC26596.1|  putative protease [Porphyromonas ...   592   e-168
 gi|34540580|ref|NP_905059.1|  ISPg3, transposase [Porphyromo...   591   e-167
 gi|34540773|ref|NP_905252.1|  ISPg3, transposase [Porphyromo...   591   e-167
 gi|34540105|ref|NP_904584.1|  ISPg3, transposase [Porphyromo...   588   e-166
 gi|34540050|ref|NP_904529.1|  ISPg3, transposase [Porphyromo...   587   e-166
 gi|150009840|ref|YP_001304583.1|  putative transposase [Para...   303   7e-81
 gi|150002628|ref|YP_001297372.1|  putative transposase [Bact...   293   1e-77
 gi|89142213|ref|YP_512234.1|  transposase [Pasteurella treha...   282   2e-74


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [113-279]T 1.3999989204989E-32 PF01609 Transposase_11 Transposase_11
InterPro
IPR012337
Domain
Polynucleotidyl transferase, Ribonuclease H fold
SSF53098 [193-280]T 0.014 SSF53098 RNaseH_fold RNaseH_fold


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 212-286 are 96% similar to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190030 which is seen in O87010_PORGI.

Residues 135-208 are identical to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in O87010_PORGI.

Residues 75-133 are 94% similar to a (PROTEASE PUTATIVE TRANSPOSASE PLASMID) protein domain (PD190029 which is seen in O87010_PORGI.

Residues 1-74 are 98% similar to a (PUTATIVE PROTEASE PROTEASE) protein domain (PD203593 which is seen in O87010_PORGI.

Residues 116-280 are 30% similar to a (HYPOTHETICAL 20.1 KD PROTEIN) protein domain (PD207068 which is seen in Q06444_BACST.


Paralogs:  Local Blast Search
PG1115 is essentially identical to PG0718, PG0236, PG0178 and PG1115, ISPg3 transposases. PG1115 is similar to fragments of PG1663 and PG1328, ISPg3 transposases, and to PG1660 and PG0273, which are ISPg3 transposase fragments.

Pfam Summary:  Pfam Search
Residues 113 to 279 (E-value = 4.9e-36) place PG1115 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)

 Structural Feature(s):
Feature Type  Start  Stop
coil-coil  
220  
255

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MKTNIVDVFCIIDDFSKLFDETIKKKTLEEADKKCRNRKFKMSDSEVMTI
LILFHLSRYRDLKAFYLQYITHSCRSEFPHLVSYNRFVELQSRGGGKLIA
FLNMCCLGQCTGISFIDSTPLKVCHIKRAHGHRTMRGWAQKGKSTMGWFY
GFKLHIVINDRGEIINYQITPGNCDDREPLKDGTFTKNLFGKLIADRGYI
SQNLFDRLFVDDIHMITKIKKNMKNSLMHLYDKVLLRKRALIETVNDILK
NLCRIEHTRHRSVNNFVTNLISGIIAYNILPKKPELNIEIIRNPNFPISA


Gene Nucleotide Sequence:  Sequence Viewer
ATGAAGACAAATATAGTTGATGTTTTTTGCATCATAGATGATTTCTCCAA
GCTTTTTGATGAAACAATCAAGAAAAAGACCCTCGAAGAGGCAGACAAAA
AATGCAGGAATAGAAAGTTTAAGATGTCGGACAGTGAGGTCATGACCATC
CTGATCCTGTTTCATCTGTCAAGATACCGAGATTTGAAAGCTTTTTATCT
TCAATACATCACCCATTCTTGTCGATCCGAGTTTCCACATCTTGTCTCTT
ATAATCGCTTTGTGGAGCTGCAAAGCAGGGGGGGGGGCAAGCTGATAGCA
TTTCTCAATATGTGTTGTTTGGGTCAATGTACAGGCATCTCTTTCATCGA
TTCCACCCCACTGAAGGTTTGTCATATCAAACGAGCTCATGGGCATAGGA
CAATGAGGGGATGGGCTCAAAAAGGCAAAAGCACCATGGGTTGGTTTTAT
GGATTCAAACTACATATTGTTATCAACGACAGGGGTGAAATCATCAACTA
TCAAATCACACCGGGCAATTGTGATGACAGAGAACCTCTGAAAGACGGAA
CATTCACCAAGAATCTTTTTGGCAAACTCATTGCCGATAGAGGCTACATT
TCCCAAAACCTTTTTGACCGGCTCTTTGTCGATGACATCCACATGATAAC
CAAAATCAAAAAGAACATGAAGAACTCCCTGATGCATCTATATGACAAAG
TTTTATTGAGAAAGAGAGCCTTGATCGAAACGGTCAATGATATACTCAAA
AATCTCTGTCGGATAGAGCACACGAGACATCGCAGTGTCAACAATTTTGT
CACCAACCTGATCTCCGGTATCATCGCTTACAACATCCTGCCTAAAAAGC
CTGAACTCAATATTGAAATCATCAGAAACCCTAACTTTCCTATTTCCGCT

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