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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1015 IGR1019 IGR1020 IGR1014 IGR1018 IGR1016 IGR1017 IGR1013 PG1186 PG1190 pyrE, - PG1188 ISPg1, - PG1184 PG1189 PG1182 PG1183 PG1180 ISPg2, - PG1185 wcfI, - PG1181 PG1186 PG1190 pyrE, - PG1188 ISPg1, - PG1184 PG1189 PG1182 PG1183 PG1180 ISPg2, - PG1185 wcfI, - PG1181 Type: IS element, Name: ISPg1 fragment - 3 Type: IS element, Name: ISPg1 fragment - 3 Type: IS element, Name: ISPg2 - 4 pyrE, - PG1188 ISPg1, - PG1184 PG1189 PG1187 PG1186 PG1190 PG1187 PG1182 PG1183 PG1180 ISPg2, - PG1185 wcfI, - PG1181


LANL Gene ID: PG1184

GenBank Locus Tag: PG1349

DNA Molecule Name:
1  

Genomic Island ID:
24

GenBank ID:


 Gene Name:
ISPg1  

 Definition:
ISPG1 transposase fragment (IS1106-related transposase)

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
1430663

Gene Stop:
1429887

Gene Length:
777

Molecular Weight*:
29697

pI*:
10.10

Net Charge*:
10.52

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0006313    transposition, DNA-mediated

Molecular function
  GO:0003677    DNA binding
  GO:0004803    transposase activity


 Pathway: pathway table

 Primary Evidence:
Hanley,S.A., Aduse-Opoku,J. and Curtis,M.A.
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis
W50 has arisen via horizontal gene transfer
Infect. Immun. 67 (3), 1157-1171 (1999)

Maley,J. and Roberts,I.S.
Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements
Microbiol. Lett. 123 (1-2), 219-224 (1994)

Shibata,Y., Hayakawa,M., Takiguchi,H., Shiroza,T. and Abiko,Y.
Determination and characterization of the hemagglutinin-associated
short motifs found in Porphyromonas gingivalis multiple gene
products. J. Biol. Chem. 274 (8), 5012-5020 (1999)

 Secondary Evidence:
Knight,A.I., Ni,H., Cartwright,K.A. and McFadden,J.J.
Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidis.
Mol. Microbiol. 6 (11), 1565-1573 (1992)

 Comment:
Member of the IS5 family of elements. Reproduces the middle and C-terminal domains of full-length ISPg1 elements (e.g. PG1662).

TIGR ID: PG1349

 Proteomic Data: Proteomic Data Search
TIGR AnnotationISPg1, transposase, degenerate, GroupG (disrupted reading frame) ,GroupL (other categories)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG13492.330.0388.278.8e-083.030.00818.278.8e-08
LANLPG11845.04307.738.19520.50
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG1184 is equivalent to the middle and C-terminal regions of the previously sequenced AJ130872 in GenBANK. See also AB015879. Residues 6-248 are 35% similar to the IS1106 transposase from N.meningitidis (AE002488).

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|4001716|dbj|BAA35090.1|  ORF7 [Porphyromonas gingivalis]       491   e-137
 gi|3901097|emb|CAA10225.1|  transposase [Porphyromonas gingi...   491   e-137
 gi|34540041|ref|NP_904520.1|  ISPg1, transposase [Porphyromo...   490   e-137
 gi|34540279|ref|NP_904758.1|  ISPg1, transposase [Porphyromo...   488   e-136
 gi|1361314|pir||S57895  hypothetical protein 1 - Porphyromon...   365   1e-99
 gi|91218509|ref|ZP_01255448.1|  ISPg1, transposase [Psychrof...   221   3e-56
 gi|91216745|ref|ZP_01253710.1|  ISPg1, transposase [Psychrof...   162   2e-38
 gi|121635472|ref|YP_975717.1|  putative transposase for IS11...   148   3e-34
 gi|121634170|ref|YP_974415.1|  putative transposase for IS11...   147   7e-34
 gi|15677391|ref|NP_274546.1|  IS1106 transposase [Neisseria ...   147   9e-34


InterPro Summary:  InterProScan

InterPro
IPR002559
Domain
Transposase, IS4-like
PF01609 [45-246]T 3e-40 PF01609 Transposase_11 Transposase_11


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 201-259 are identical to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187845 which is seen in Q9ZA61_PORGI.

Residues 55-200 are 87% similar to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in Q9ZA61_PORGI.

Residues 8-54 are identical to a (TRANSPOSASE ORF7 INSERTION SEQUENCE) protein domain (PD187846 which is seen in Q9ZA61_PORGI.


Paralogs:  Local Blast Search
PG1184 is virtually identical to PG1087, PG0763, PG1755, PG0686, PG0756, PG1798, PG1230, PG1919, PG0044, PG0689, PG1215, PG1665, PG0839, PG1895, all ISPg1 fragments that reproduce the middle and C-terminal residues of PG1267, PG0169, PG1050, PG1062, PG0740, PG0918, PG1662, PG1422, PG0420, PG0499, PG1099.

Also, partial similarities to PG0732, PG0842, PG0883, PG1231, PG0730, PG1159, PG1608.

Pfam Summary:  Pfam Search
Residues 45 to 246 (E-value = 2.2e-40) place PG1184 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)

 Structural Feature(s):
Feature Type  Start  Stop
non-globular  
36  
133
coil-coil  
182  
211

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MEEVSPDHSTISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLVDA
SLVETPHKPNGSITIEVADDREDNRSEEEKEAEEDYQKQVVRQRKGTDEE
ARWVYKQKRYHYGYKKHCLTNVQGIVQKVITTAANRSDTKEFIALLQGAN
IPQGSAVLADKGYACGENRSYLQTHHLQDGIMHKAQRNRALTEEEKQRNK
AISRIRSTIERTFGSIRRWFHGGRCRYRGLAKTHTQNILESIAFNLYRTP
GIIMSSFVG

Gene Nucleotide Sequence:  Sequence Viewer
ATGGAAGAGGTATCTCCCGACCACAGCACCATCAGTCGATTTCGTTCGGC
ACTGACAGAGTTGGGGCTCATGGACAAACTATTGGCGCAGTTTAACAAAC
AACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTCGATGCA
AGCCTTGTGGAGACGCCGCATAAACCCAACGGAAGCATTACGATTGAAGT
CGCAGACGACAGAGAAGACAATCGGAGCGAGGAGGAAAAAGAGGCAGAGG
AGGATTATCAAAAACAGGTTGTCCGTCAGCGTAAAGGGACGGATGAAGAA
GCCCGTTGGGTTTACAAACAAAAGCGTTATCACTACGGATACAAAAAGCA
TTGTCTGACCAATGTTCAAGGCATTGTTCAAAAGGTGATAACGACTGCAG
CGAACCGCAGTGACACGAAGGAGTTTATTGCGCTATTGCAGGGTGCAAAC
ATACCTCAAGGCTCAGCCGTCTTGGCGGACAAAGGATATGCTTGCGGGGA
AAATCGTTCCTACCTGCAAACCCATCACCTTCAAGACGGCATCATGCACA
AGGCACAACGCAACAGGGCATTGACCGAGGAAGAGAAGCAACGAAACAAA
GCAATCAGTCGGATACGGAGCACCATCGAACGCACCTTTGGCAGTATTCG
CCGGTGGTTTCATGGCGGACGATGTCGATACCGGGGACTTGCCAAGACCC
ATACTCAAAACATTCTTGAAAGCATCGCCTTTAATTTATACAGAACCCCG
GGGATAATTATGTCCTCATTTGTAGGA

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