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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1024 IGR1025 IGR1026 IGR1027 IGR1023 IGR1022 PG1200 PG1193 PG1194 dxr, - PG1199 purD, - PG1195 PG1197 Ptp-A, - PG1196 PG1192 PG1198 PG1200 PG1193 PG1194 dxr, - PG1199 purD, - PG1195 PG1197 Ptp-A, - PG1196 PG1192 PG1198 PG1200 PG1193 PG1194 dxr, - PG1199 purD, - PG1195 PG1197 Ptp-A, - PG1196 PG1192 PG1198


LANL Gene ID: PG1196

GenBank Locus Tag: PG1361

DNA Molecule Name:
1  

GenBank ID:
34541047

Gene Name:
Ptp-A  

Definition:
prolyl tripeptidyl peptidase (Ptp-A)

Cellular Location:
Periplasm, Cytoplasm, Extracellular [Evidence]

Gene Start:
1442201

Gene Stop:
1440006

Gene Length:
2196

Molecular Weight*:
82260

pI*:
6.60

Net Charge*:
-4.79

EC:
 

Functional Class:
Protein fate; Degradation of proteins, peptides and glycopeptides  

Gene Ontology:
Biological process
  GO:0006508    proteolysis

Cellular component
  GO:0016020    membrane

Molecular function
  GO:0004274    dipeptidyl-peptidase IV activity
  GO:0008236    serine-type peptidase activity


Pathway: pathway table

Primary Evidence:
Banbula A, Mak P, Bugno M, Silberring J, Dubin A, Nelson D, Travis J, Potempa J. Prolyl tripeptidyl peptidase from Porphyromonas gingivalis. A novel enzyme with possible pathological implications for the development of periodontitis.
J Biol Chem. 1999 Apr 2;274(14):9246-52.
PMID: 10092598

Secondary Evidence:
Kabashima,T., Yoshida,T., Ito,K. and Yoshimoto,T. 1995. Cloning, sequencing, and expression of the dipeptidyl peptidase IV
gene from Flavobacterium meningosepticum in Escherichia coli. Arch. Biochem. Biophys. 320 (1): 123-128. PubMed: 7793970.

Kiyama,M., Hayakawa,M., Shiroza,T., Nakamura,S., Takeuchi,A.,
Masamoto,Y. and Abiko,Y. 1998. Sequence analysis of the Porphyromonas gingivalis dipeptidyl peptidase IV gene. Biochim. Biophys. Acta 1396 (1): 39-46. PubMed: 9524216.
Kumagai,Y., Konishi,K., Gomi,T., Yagishita,H., Yajima,A. and
Yoshikawa,M. 2000. Enzymatic properties of dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis and its participation in virulence. Infect. Immun. 68 (2): 716-724. PubMed: 10639438



Comment:
Banbula et al. (1999) demonstrate tripeptidyl peptidase activity for this enzyme, though it lacks structural similarity to other known tripeptidyl peptidases. True DPP-IV enzymes from their point of view are apparently represented by PG0459, PG1528, PG0652.

Unfortunately ptpA also denotes PG1433, a predicted protein-tyrosine-phosphatase.

TIGR ID: PG1361

Proteomic Data: Proteomic Data Search
TIGR Annotationdipeptidyl aminopeptidase IV, putative, GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG1361-0.360.0052-0.430.00630.170.083-0.430.0063
LANLPG11960.780.741.120.91
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Residues 154-732 of PG1196 are similar to previously sequenced P.gingivalis proteins in Genbank, AAC46184 and BAA28265.

Numerous significant hits to dipeptidyl peptidase proteins in gapped BLAST; e.g. residues 154-732 are 34% similar to prf||2115194A dipeptidyl peptidase IV of Flavobacterium meningosepticum, residues 155-721 are 34% similar to pir||JC5142 X-Pro dipeptidyl-peptidase of Xanthomonas maltophilia, residues 155-732 are 30% similar to dbj|BAB10391.1| dipeptidyl peptidase IV-like protein of Arabidopsis thaliana.

This sequence is similar to BT3254.


 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34541047|ref|NP_905526.1|  dipeptidyl aminopeptidase IV, ...  1544   0.0  
 gi|116667018|pdb|2DCM|A  Chain A, The Crystal Structure Of S...  1464   0.0  
 gi|116667002|pdb|2D5L|A  Chain A, Crystal Structure Of Proly...  1464   0.0  
 gi|154493409|ref|ZP_02032729.1|  hypothetical protein PARMER...   769   0.0  
 gi|150009679|ref|YP_001304422.1|  dipeptidyl peptidase IV [P...   749   0.0  
 gi|60679693|ref|YP_209837.1|  putative exported dipeptidyl p...   672   0.0  
 gi|53711376|ref|YP_097368.1|  dipeptidyl peptidase IV [Bacte...   669   0.0  
 gi|150003663|ref|YP_001298407.1|  dipeptidyl peptidase IV [B...   659   0.0  
 gi|156859118|gb|EDO52549.1|  hypothetical protein BACUNI_041...   652   0.0  
 gi|29348663|ref|NP_812166.1|  dipeptidyl peptidase IV [Bacte...   626   e-177


InterPro Summary:  InterProScan

InterPro
IPR001375
Domain
Peptidase S9, prolyl oligopeptidase active site region
PF00326 [532-732]T 1.2000011745813998E-43 PF00326 Peptidase_S9 Peptidase_S9
InterPro
IPR002469
Domain
Peptidase S9B, dipeptidylpeptidase IV N-terminal
PF00930 [115-452]T 2.0000139277226602E-128 PF00930 DPPIV_N DPPIV_N
noIPR
unintegrated
unintegrated
G3DSA:2.140.10.30 [30-468]T 4.600000443309081E-90 G3DSA:2.140.10.30 G3DSA:2.140.10.30 G3DSA:2.140.10.30
G3DSA:3.40.50.1820 [485-732]T 1.9000069431527E-73 G3DSA:3.40.50.1820 G3DSA:3.40.50.1820 G3DSA:3.40.50.1820
PTHR11731 [154-732]T 0.0 PTHR11731 PTHR11731 PTHR11731
PTHR11731:SF12 [154-732]T 0.0 PTHR11731:SF12 PTHR11731:SF12 PTHR11731:SF12
SSF53474 [479-732]T 9.1E-66 SSF53474 SSF53474 SSF53474
SSF82171 [30-481]T 8.0E-72 SSF82171 SSF82171 SSF82171


COGS Summary:  COGS Search
BeTs to 4 clades of COG1506
COG name: Dipeptidyl aminopeptidases/acylaminoacyl-peptidases
Functional Class: E
The phylogenetic pattern of COG1506 is ---KY--c-b-----------
Number of proteins in this genome belonging to this COG is 5
Blocks Summary:  Blocks Search
***** PF00930 (Dipeptidyl peptidase IV (DPP IV) N-terminal region) with a combined E-value of 3.9e-48.
    PF00930B    182-232
    PF00930C    243-269
    PF00930E    376-386
    PF00930F    425-470
    PF00930G    502-539
    PF00930H    542-584
    PF00930I    620-647
    PF00930J    669-689
***** IPB002471 (Prolyl endopeptidase family serine active site) with a combined E-value of 4.9e-08.
    IPB002471A    69-81
    IPB002471B    578-608


 ProDom Summary:  Protein Domain Search
Residues 601-727 are 41% similar to a (DIPEPTIDYL HYDROLASE PROTEIN IV PEPTIDASE PROTEASE) protein domain (PD003048 which is seen in Q47900_FLAME.

Residues 154-385 are 33% similar to a (DIPEPTIDYL IV HYDROLASE PROTEASE) protein domain (PD003086 which is seen in Q47900_FLAME.

Residues 477-599 are 49% similar to a (DIPEPTIDYL HYDROLASE IV PROTEIN PEPTIDASE PROTEASE) protein domain (PD001060 which is seen in P95782_BBBBB.


Paralogs:  Local Blast Search
Residues 154-732 are 31% similar to PG0459, a predicted dipeptidyl peptidase IV (DPP IV) N-terminal region. Similarites are also seen to PG1528, PG0652, PG0897.

Pfam Summary:  Pfam Search
Residues 34 to 525 (E-value = 1.1e-35) place PG1196 in the DPPIV_N family which is described as Dipeptidyl peptidase IV (DPP IV) N-terminal region (PF00930)
Residues 532 to 608 (E-value = 2.1e-06) place PG1196 in the Peptidase_S9 family which is described as Prolyl oligopeptidase family (PF00326)

 Structural Feature(s):
Feature Type  Start  Stop
gram negative signal  
1  
24
cleavable signal  
1  
31
transmembrane  
120  
136

Top PDB Hits:
-43% similar to PDB:1ORV Crystal Structure of Porcine Dipeptidyl Peptidase IV (CD26) (E_value = 1.3E_43);
-43% similar to PDB:1ORW Crystal Structure of Porcine Dipeptidyl Peptidase IV (CD26) in Complex with a Peptidomimetic Inhibitor (E_value = 1.3E_43);
-43% similar to PDB:2AJ8 Porcine dipeptidyl peptidase IV (CD26) in complex with 7-Benzyl-1,3-dimethyl-8-piperazin-1-yl-3,7-dihydro-purine-2,6-dione (BDPX) (E_value = 1.3E_43);
-43% similar to PDB:2AJB Porcine dipeptidyl peptidase IV (CD26) in complex with the tripeptide tert-butyl-Gly-L-Pro-L-Ile (tBu-GPI) (E_value = 1.3E_43);
-43% similar to PDB:2AJC Porcine dipeptidyl peptidase IV (CD26) in complex with 4-(2-Aminoethyl)-benzene sulphonyl fluoride (AEBSF) (E_value = 1.3E_43);

Gene Protein Sequence:
MKKTIFQQLFLSVCALTVALPCSAQSPETSGKEFTLEQLMPGGKEFYNFY
PEYVVGLQWMGDNYVFIEGDDLVFNKANGKSAQTTRFSAADLNALMPEGC
KFQTTDAFPSFRTLDAGRGLVVLFTQGGLVGFDMLARKVTYLFDTNEETA
SLDFSPVGDRVAYVRNHNLYIARGGKLGEGMSRAIAVTIDGTETLVYGQA
VHQREFGIEKGTFWSPKGSCLAFYRMDQSMVKPTPIVDYHPLEAESKPLY
YPMAGTPSHHVTVGIYHLATGKTVYLQTGEPKEKFLTNLSWSPDENILYV
AEVNRAQNECKVNAYDAETGRFVRTLFVETDKHYVEPLHPLTFLPGSNNQ
FIWQSRRDGWNHLYLYDTTGRLIRQVTKGEWEVTNFAGFDPKGTRLYFES
TEASPLERHFYCIDIKGGKTKDLTPESGMHRTQLSPDGSAIIDIFQSPTV
PRKVTVTNIGKGSHTLLEAKNPDTGYAMPEIRTGTIMAADGQTPLYYKLT
MPLHFDPAKKYPVIVYVYGGPHAQLVTKTWRSSVGGWDIYMAQKGYAVFT
VDSRGSANRGAAFEQVIHRRLGQTEMADQMCGVDFLKSQSWVDADRIGVH
GWSYGGFMTTNLMLTHGDVFKVGVAGGPVIDWNRYEIMYGERYFDAPQEN
PEGYDAANLLKRAGDLKGRLMLIHGAIDPVVVWQHSLLFLDACVKARTYP
DYYVYPSHEHNVMGPDRVHLYETITRYFTDHL

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAGAAGACAATCTTCCAACAACTATTTCTGTCTGTTTGTGCCCTTAC
AGTGGCCTTGCCTTGTTCGGCTCAGTCTCCTGAAACGAGTGGTAAGGAGT
TTACTCTTGAGCAACTGATGCCCGGAGGAAAAGAGTTTTATAACTTTTAC
CCCGAATACGTGGTCGGTTTGCAATGGATGGGAGACAATTATGTCTTTAT
CGAGGGTGATGATTTAGTTTTTAATAAGGCGAATGGCAAATCGGCTCAGA
CGACCAGATTTTCTGCTGCCGATCTCAATGCACTCATGCCGGAGGGATGC
AAATTTCAGACGACTGATGCTTTCCCTTCATTCCGCACACTCGATGCCGG
ACGGGGACTGGTCGTTCTATTTACCCAAGGAGGATTAGTCGGATTCGATA
TGCTTGCTCGAAAGGTGACTTATCTTTTCGATACCAATGAGGAGACGGCT
TCTTTGGATTTTTCTCCTGTGGGAGACCGTGTTGCCTATGTCAGAAACCA
TAACCTTTACATTGCTCGTGGAGGTAAATTGGGAGAAGGTATGTCACGAG
CTATCGCTGTGACTATCGATGGAACTGAGACTCTCGTATATGGCCAGGCC
GTACACCAGCGTGAATTCGGTATCGAAAAAGGTACATTCTGGTCTCCAAA
AGGGAGCTGCCTTGCTTTCTATCGAATGGATCAGAGTATGGTGAAGCCTA
CCCCGATAGTGGATTATCATCCGCTCGAAGCCGAGTCCAAACCGCTTTAT
TACCCCATGGCAGGTACTCCGTCACACCACGTTACGGTTGGGATCTATCA
TCTGGCCACAGGTAAGACCGTCTATCTACAAACGGGTGAACCCAAGGAAA
AATTTCTGACGAATTTGAGTTGGAGTCCGGACGAAAATATCTTGTATGTA
GCTGAGGTGAATCGTGCTCAAAACGAATGTAAGGTAAATGCCTATGACGC
TGAGACCGGTAGATTCGTCCGTACGCTTTTTGTTGAAACCGATAAACATT
ATGTAGAGCCGTTACATCCCCTGACATTCCTTCCGGGAAGTAACAATCAG
TTCATTTGGCAGAGCCGTCGCGACGGATGGAACCATCTCTATCTGTATGA
TACTACAGGTCGTCTGATCCGTCAGGTGACAAAAGGGGAGTGGGAGGTTA
CAAACTTTGCAGGCTTCGATCCCAAGGGAACACGGCTCTATTTCGAAAGT
ACCGAAGCCAGCCCTCTCGAACGCCATTTTTACTGTATTGATATCAAAGG
AGGAAAGACAAAAGATCTGACTCCGGAGTCGGGAATGCACCGCACTCAGC
TATCTCCTGATGGTTCTGCCATAATCGATATTTTTCAGTCACCTACTGTC
CCGCGTAAGGTTACAGTGACAAATATCGGCAAAGGGTCTCACACACTCTT
GGAGGCTAAGAACCCCGATACGGGCTATGCCATGCCGGAGATCAGAACGG
GTACCATCATGGCGGCCGATGGGCAGACACCTCTTTATTACAAGCTCACG
ATGCCGCTTCATTTCGATCCGGCAAAGAAATATCCTGTTATTGTCTATGT
TTACGGAGGACCTCATGCCCAACTCGTAACCAAGACATGGCGCAGCTCTG
TCGGTGGATGGGATATCTATATGGCACAGAAAGGCTATGCCGTCTTTACG
GTGGATAGTCGCGGATCTGCCAATAGAGGGGCTGCTTTCGAGCAGGTTAT
TCATCGTCGTTTGGGGCAGACCGAGATGGCCGATCAGATGTGCGGTGTGG
ATTTCCTCAAGAGCCAATCATGGGTGGATGCCGATAGAATAGGAGTACAT
GGCTGGAGCTATGGTGGCTTTATGACTACGAATCTGATGCTTACGCACGG
CGATGTCTTCAAAGTCGGAGTAGCCGGCGGGCCTGTCATAGACTGGAATC
GATATGAGATTATGTACGGTGAGCGTTATTTCGATGCGCCACAGGAAAAT
CCCGAAGGATACGATGCTGCCAACCTGCTCAAACGAGCCGGTGATCTGAA
AGGACGACTTATGCTGATTCATGGAGCGATCGATCCGGTCGTGGTATGGC
AGCATTCACTCCTTTTCCTTGATGCTTGCGTGAAGGCACGCACCTATCCT
GACTATTACGTCTATCCGAGCCACGAACATAATGTGATGGGGCCGGACAG
AGTACATTTGTATGAAACAATAACCCGTTATTTCACAGATCACTTA

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