LANL Gene ID: PG1215
GenBank Locus Tag: PG1384
DNA Molecule Name:
1
Genomic Island ID:
25
GenBank ID:
Gene Name:
ISPg1
Definition:
ISPg1 transposase fragment (IS1106-related transposase)
Cellular Location:
Cytoplasm [Evidence]
Gene Start:
1464183
Gene Stop:
1464959
Gene Length:
777
Molecular Weight*:
29537
pI*:
9.80
Net Charge*:
8.52
EC:
Functional Class:
Transposon functions
Gene Ontology:
Biological process
GO:0006313 transposition, DNA-mediated
Molecular function
GO:0003677 DNA binding
GO:0004803 transposase activity
Pathway: pathway table
Primary Evidence:
Hanley,S.A., Aduse-Opoku,J. and Curtis,M.A.
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis
W50 has arisen via horizontal gene transfer
Infect. Immun. 67 (3), 1157-1171 (1999)
Maley,J. and Roberts,I.S.
Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements
Microbiol. Lett. 123 (1-2), 219-224 (1994)
Shibata,Y., Hayakawa,M., Takiguchi,H., Shiroza,T. and Abiko,Y.
Determination and characterization of the hemagglutinin-associated
short motifs found in Porphyromonas gingivalis multiple gene
products. J. Biol. Chem. 274 (8), 5012-5020 (1999)
Secondary Evidence:
Knight,A.I., Ni,H., Cartwright,K.A. and McFadden,J.J.
Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidis.
Mol. Microbiol. 6 (11), 1565-1573 (1992)
Comment:
Member of the IS5 family of elements. Reproduces the middle and C-terminal domains of full-length ISPg1 elements (e.g. PG1662).
TIGR ID: PG1384
Human Oral Microbiome Database:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
PG1215 is equivalent to the middle to C-terminal region (residues 103-361) of the previously sequenced AB015879 in GenBANK. See also AJ130872. Residues 6-248 are 34% similar to the IS1106 from N. meningitidis (AE002488).
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
InterPro Summary: InterProScan
| InterPro IPR002559 Domain 
|
| |||||||
![[45-246]T 5.1e-36 PF01609 Transposase_11](http://www.ebi.ac.uk/InterProScan/images/H.gif){kind=small}
COGS Summary: COGS Search
No hit to the COGs database.
Blocks Summary: Blocks Search
No significant hit to the Blocks database.
ProDom Summary: Protein Domain Search
Residues 201-259 are 98% similar to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187845 which is seen in Q9ZAD0_PORGI.
Residues 55-200 are 85% similar to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in Q9ZA61_PORGI.
Residues 8-54 are identical to a (TRANSPOSASE ORF7 INSERTION SEQUENCE) protein domain (PD187846 which is seen in Q9ZA61_PORGI.
Paralogs: Local Blast Search
PG1215 is virtually identical to PG1087, PG0763, PG1755, PG0686, PG0756, PG1798, PG1230, PG1919, PG0044, PG0689, PG1184, PG1665, PG0839, PG1895, all ISPg1 fragments that reproduce the middle and C-terminal residues of PG1267, PG0169, PG1050, PG1062, PG0740, PG0918, PG1662, PG1422, PG0420, PG0499, PG1099.
Also, partial similarities to PG0732, PG0842, PG0883, PG1231, PG0730, PG1159, PG1608.
Pfam Summary: Pfam Search
Residues 45 to 246 (E-value = 4.1e-36) place PG1215 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)
Structural Feature(s):
| Feature Type | Start | Stop |
| non-globular |
Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).
Gene Protein Sequence:
MEEVSPDHSTISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLVDA
SLVETPHKPNGSITIEVADDREDNRSEEEKEAEEDYQKQVVRQRKGTDEE
ARWVYKQKRYHYGYKKHCLTNVQGIVQKVITTAANRSDTKEFIALLQGAN
IPQGTAVLADKGYACGENRFYLQTHHLQDGIMHKAQRNRALTEEEKQGNK
AIGPIRSTIERTFGSIRRWFHGGRCRYRGLAKTHTQNILESIAFNLYRTP
GIIMSSSLG
Gene Nucleotide Sequence: Sequence Viewer
ATGGAAGAGGTATCTCCCGACCACAGCACCATCAGTCGATTTCGTTCGGC
ACTGACAGAGTTGGGGCTCATGGACAAACTATTGGCGCAGTTTAACAAAC
AACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTCGATGCA
AGCCTTGTGGAGACGCCGCATAAACCCAACGGAAGCATTACGATTGAAGT
CGCAGACGACAGAGAAGACAATCGGAGCGAGGAGGAAAAAGAGGCAGAGG
AGGATTATCAAAAACAGGTTGTCCGTCAGCGTAAAGGGACGGATGAAGAA
GCCCGTTGGGTTTACAAACAAAAGCGTTATCACTACGGATACAAAAAGCA
TTGTCTGACCAATGTTCAAGGCATTGTTCAAAAGGTGATAACGACTGCAG
CGAACCGCAGTGACACGAAGGAGTTTATTGCGCTATTGCAGGGTGCAAAC
ATACCTCAAGGCACAGCCGTCTTGGCGGACAAAGGATATGCTTGCGGGGA
AAATCGTTTCTACCTGCAAACCCATCACCTTCAAGACGGCATCATGCACA
AGGCACAACGCAACAGGGCATTGACCGAGGAAGAGAAGCAAGGAAACAAA
GCAATCGGTCCGATACGGAGCACCATCGAACGCACCTTTGGCAGTATTCG
CCGGTGGTTTCATGGCGGACGATGTCGATACCGGGGACTTGCCAAGACCC
ATACTCAAAACATTCTTGAAAGCATCGCCTTTAATTTATACAGAACCCCG
GGGATAATTATGTCCTCATCTCTAGGA