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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1072 IGR1070 IGR1071 IGR1075 IGR1074 IGR1069 IGR1073.1 IGR1073 PG1247.1 dacB,pbp, - PG1248 arcA,pad, - PG1249 ISPg5, - PG1246 ISPg5, - PG1247 PG1243 fumA,fumB, - PG1244 dnaX, - PG1245 PG1247.1 dacB,pbp, - PG1248 arcA,pad, - PG1249 ISPg5, - PG1246 ISPg5, - PG1247 PG1243 fumA,fumB, - PG1244 dnaX, - PG1245 Type: IS element, Name: ISPg5 - 8 PG1247.1 dacB,pbp, - PG1248 arcA,pad, - PG1249 ISPg5, - PG1246 ISPg5, - PG1247 PG1243 fumA,fumB, - PG1244 dnaX, - PG1245


LANL Gene ID: PG1247

GenBank Locus Tag: PG1420

DNA Molecule Name:
1  

GenBank ID:
34541096

Gene Name:
ISPg5  

Definition:
ISPg5 transposase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1505589

Gene Stop:
1506524

Gene Length:
936

Molecular Weight*:
36207

pI*:
10.00

Net Charge*:
15.47

EC:
 

Functional Class:
Transposon functions  

Gene Ontology:
Biological process
  GO:0015074    DNA integration

Molecular function
  GO:0003677    DNA binding


Pathway: pathway table

Primary Evidence:
Califano,J.V., Kitten,T., Lewis,J.P., Macrina,F.L., Fleischmann,R.D., Fraser,C.M.,Duncan,M.J. and Dewhirst,F.E. Characterization of Porphyromonas gingivalis insertion
sequence-like element ISPg5.
Infect. Immun. 68 (9), 5247-5253 (2000)

Comment:
One of eleven ISPg5 elements in the genome (see Paralogs). Califano et al. report considerable strain diversity of these (Infect. Immun. 68 (9), 5247-5253 (2000)).

Member of the IS3 family of insertion elements. See PG1246 for associated ORF.

TIGR ID: PG1420

Human Oral Microbiome Database:
View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
PG1247 is equivalent to the entire length of the protein previously reported gb|AAF69128.1| in GenBANK.

Numerous significant hits in gapped BLAST to transposase proteins; e.g. residues 91-213 are 56% similar to transposase homolog (U67062) of Bacteriodes thetaiotaomicrom, residues 64-287 are 32% similar to InsB (AF153317) of Shigella dysenteriae, residues 64-268 are 33% similar to Hp1 (AF081284) of E.coli.

Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34540696|ref|NP_905175.1|  ISPg5, transposase Orf2 [Porph...   627   e-178
 gi|118200270|dbj|BAF37077.1|  transposase Orf2 [Porphyromona...   622   e-176
 gi|34540399|ref|NP_904878.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34541641|ref|NP_906120.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34539915|ref|NP_904394.1|  ISPg5 transposase Orf2 [Porphy...   617   e-175
 gi|34540277|ref|NP_904756.1|  ISPg5, transposase Orf2 [Porph...   615   e-174
 gi|34541341|ref|NP_905820.1|  ISPg5, transposase Orf2 [Porph...   610   e-173
 gi|34540248|ref|NP_904727.1|  ISPg5, transposase Orf2 [Porph...   609   e-173
 gi|34541287|ref|NP_905766.1|  ISPg5, transposase Orf2 [Porph...   607   e-172
 gi|34539888|ref|NP_904367.1|  ISPg5 transposase Orf2 [Porphy...   606   e-172


InterPro Summary:  InterProScan

InterPro
IPR001584
Domain
Integrase, catalytic core
PF00665 [110-273]T 6.5e-32 PF00665 rve rve
PS50994 [110-276]T 18.646 PS50994 INTEGRASE INTEGRASE
noIPR
unintegrated
unintegrated
G3DSA:3.30.420.10 [113-281]T 7.5e-07 G3DSA:3.30.420.10 no description no description


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01836 (Transposase) with a combined E-value of 4.3e-23.
    PF01836B    103-154
    PF01836C    181-190
    PF01836D    215-222
    PF01836E    253-272


 ProDom Summary:  Protein Domain Search
No significant hit to the ProDom database.

Paralogs:  Local Blast Search
PG1247 is essentially identical to PG0841, PG1796, PG0533, and PG0034, all ISPg5 transposases. PG1247 is paralogous to PG1861, PG1491, PG0391, PG0418, PG1436, PG0007 and PG0841, all ISPg5 transposases.

Pfam Summary:  Pfam Search
Residues 110 to 273 (E-value = 5.7e-32) place PG1247 in the rve family which is described as Integrase core domain (PF00665)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
LLGFSRQAFYKRHLNDLARHEEDVLCSSIIQYCWHLRQAEHLPQAGFREL
MVLCQQYFGPKFTLGRDRFCALLRRHGMMLRKRSVRPQTTNSRHRLYKYE
DLLNTEPKFVPQRPGELLVADITYVAYQDGFAYLSLLTDAYSRCIVGYCL
HPTLEVEGCLNALHQAFAFYDQHQIDTSNMIHHSDRGIQYASKSYTDLLH
GRGCRISMTQTGDPLHNALAERMNNTLKNSWHISSSKQSFDQALLSVDRA
VRMYNEARPHQALGAKTPMQVIAPESKNPLLTRREHGPEIAPELYRRMNV
RQRANFARVNRN

Gene Nucleotide Sequence:  Sequence Viewer
CTGCTTGGCTTTAGCCGTCAGGCCTTTTATAAACGTCATCTCAACGATTT
AGCTAGGCACGAGGAGGATGTCCTATGCAGTAGTATCATCCAATATTGTT
GGCACTTAAGACAAGCAGAGCACCTACCTCAAGCTGGCTTCCGAGAGCTC
ATGGTGCTTTGTCAGCAATACTTCGGCCCCAAGTTCACGCTTGGGCGCGA
TCGCTTTTGCGCCCTACTCAGACGCCATGGTATGATGCTGCGCAAACGGT
CTGTGCGCCCTCAGACAACCAACTCTCGGCATAGGCTCTACAAGTATGAA
GACCTGCTCAATACAGAGCCTAAGTTTGTGCCTCAAAGACCTGGGGAACT
CCTTGTAGCAGACATCACCTACGTGGCTTACCAAGATGGGTTTGCCTATC
TCTCCCTACTGACCGATGCCTATAGTCGCTGTATCGTAGGGTATTGCTTA
CATCCTACCCTAGAGGTTGAAGGCTGTTTGAATGCTCTACATCAAGCCTT
TGCTTTCTACGATCAGCATCAAATTGATACGAGCAATATGATTCATCATA
GCGACCGAGGCATTCAGTATGCCAGTAAGAGCTACACCGATCTGTTGCAT
GGGCGAGGCTGTCGCATCAGCATGACTCAGACGGGAGATCCTCTGCATAA
TGCATTGGCTGAGCGGATGAACAATACGCTCAAGAACTCGTGGCACATCT
CCTCTTCGAAGCAGTCTTTTGATCAAGCACTCCTTTCTGTAGACCGAGCC
GTGCGCATGTACAACGAGGCGCGTCCACATCAAGCTCTAGGGGCGAAAAC
GCCTATGCAAGTCATTGCGCCAGAGTCTAAAAATCCGTTACTAACGAGGA
GGGAGCATGGGCCAGAGATTGCCCCCGAGCTATACCGAAGGATGAATGTC
AGACAAAGAGCTAACTTTGCTCGTGTCAACCGAAAT

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