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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1080 IGR1078 IGR1077 IGR1075 IGR1079 IGR1074 IGR1076 ribE, - PG1252 vsrD, - PG1254 PG1253 dacB,pbp, - PG1248 arcA,pad, - PG1249 PG1255 PG1251 PG1250 ribE, - PG1252 vsrD, - PG1254 PG1253 dacB,pbp, - PG1248 arcA,pad, - PG1249 PG1255 PG1251 PG1250 Type: tandem, Name: tan50 - 258 Type: tandem, Name: tan88 - 296 Type: tandem, Name: tan49 - 257 ribE, - PG1252 vsrD, - PG1254 PG1253 dacB,pbp, - PG1248 arcA,pad, - PG1249 PG1255 PG1251 PG1250


LANL Gene ID: PG1251

GenBank Locus Tag: PG1427

DNA Molecule Name:
1  

GenBank ID:
34541102

Gene Name:


Definition:
thiol protease (PrtT related)

Cellular Location:
Outer membrane, Extracellular [Evidence]

Gene Start:
1514489

Gene Stop:
1511961

Gene Length:
2529

Molecular Weight*:
93123

pI*:
5.20

Net Charge*:
-21.25

EC:
3.4.22.-  

Functional Class:
Protein fate; Degradation of proteins, peptides and glycopeptides  

Gene Ontology:
Biological process
  GO:0006508    proteolysis

Molecular function
  GO:0008234    cysteine-type peptidase activity


Pathway: pathway table

Primary Evidence:
Otogoto,J. and Kuramitsu,H.K. 1993. Isolation and characterization of the Porphyromonas gingivalis prtT gene, coding for protease activity. Infect. Immun. 61 (1): 117-123. PubMed: 8093357.
Madden,T.E., Clark,V.L. and Kuramitsu,H.K. 1995. Revised sequence of the Porphyromonas gingivalis prtT cysteine protease/hemagglutinin gene: homology with streptococcal pyrogenic exotoxin B/streptococcal proteinase. Infect. Immun. 63 (1): 238-247. PubMed: 7806362.

Comment:
TIGR ID: PG1427

Proteomic Data: Proteomic Data Search
TIGR Annotationthiol protease/hemagglutinin PrtT precursor, putative, GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG1427-2.403.6e-06-2.469.4e-05-3.040-2.469.4e-05
LANLPG12510.190.180.120.08
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
Several hits in gapped BLAST to previously sequenced P. gingivalis sequences (PRTT_PORGI or PG1356) and to other sequences, e.g. residues 74-394 are 33% similar to exotoxin B of Strep pyogenes (L26141).

Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34541102|ref|NP_905581.1|  thiol protease/hemagglutinin P...  1694   0.0  
 gi|1172653|sp|P43158|PRTT_PORGI  Thiol protease/hemagglutini...   448   e-124
 gi|913137|gb|AAB32891.1|  PrtT [Porphyromonas gingivalis]         447   e-123
 gi|431616|gb|AAA26980.1|  pyrogenic exotoxin B [Streptococcu...   172   8e-41
 gi|55669548|pdb|1PVJ|A  Chain A, Crystal Structure Of The St...   171   1e-40
 gi|431656|gb|AAA27000.1|  pyrogenic exotoxin B [Streptococcu...   162   7e-38
 gi|431670|gb|AAA27007.1|  pyrogenic exotoxin B [Streptococcu...   162   9e-38
 gi|56807683|ref|ZP_00365564.1|  hypothetical protein SpyoM01...   162   1e-37
 gi|13591591|dbj|BAB40954.1|  pyrogenic exotoxin B [Streptoco...   162   1e-37
 gi|431622|gb|AAA26983.1|  pyrogenic exotoxin B [Streptococcu...   161   2e-37


InterPro Summary:  InterProScan

InterPro
IPR000200
Family
Peptidase C10, streptopain
PD004169 [142-394]T 0.0 PD004169 Peptidase_C10 Peptidase_C10
PR00797 [187-211]T 4.1E-39 PR00797 STREPTOPAIN[256-272]T 4.1E-39 PR00797 STREPTOPAIN[284-303]T 4.1E-39 PR00797 STREPTOPAIN[317-341]T 4.1E-39 PR00797 STREPTOPAIN[349-370]T 4.1E-39 PR00797 STREPTOPAIN[371-392]T 4.1E-39 PR00797 STREPTOPAIN STREPTOPAIN
PF01640 [4-394]T 1.69998802370796E-107 PF01640 Peptidase_C10 Peptidase_C10
noIPR
unintegrated
unintegrated
SSF54001 [55-394]T 3.9999999999999994E-116 SSF54001 SSF54001 SSF54001


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 571-807 are 24% similar to a (HEMIN-REGULATED PROTEIN THIOL PROTEASE/HEMAGGLUTININ) protein domain (PD039116 which is seen in P72198_PORGI.

Residues 119-394 are 50% similar to a (EXOTOXIN B PYROGENIC PYROGEN) protein domain (PD004169 which is seen in PRTT_PORGI.

Residues 1-118 are 29% similar to a (THIOL PROTEASE/HEMAGGLUTININ PRTT) protein domain (PD133794 which is seen in PRTT_PORGI.


Paralogs:  Local Blast Search
Residues 1-841 of PG1251 are 34% similar to PG1356, a protease/hemagglutinin PrtT protein. Weak similarities are seen to PG1838, immunoreactive antigen, and PG0592, a hypothetical protein.

Pfam Summary:  Pfam Search
Residues 1 to 394 (E-value = 1.7e-111) place PG1251 in the Peptidase_C10 family which is described as Peptidase C10 family (PF01640)

 Structural Feature(s):
Feature Type  Start  Stop
gram negative signal  
1  
21
cleavable signal  
1  
21
non-globular  
354  
470

Top PDB Hits:
-50% similar to PDB:1PVJ Crystal structure of the Streptococcal pyrogenic exotoxin B (SpeB)- inhibitor complex (E_value = 6.3E_45);
-50% similar to PDB:1DKI CRYSTAL STRUCTURE OF THE ZYMOGEN FORM OF STREPTOCOCCAL PYROGENIC EXOTOXIN B ACTIVE SITE (C47S) MUTANT (E_value = 7.0E_44);
-34% similar to PDB:1DT0 CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS (E_value = 7.0E_44);
-39% similar to PDB:1GBG BACILLUS LICHENIFORMIS BETA-GLUCANASE (E_value = 7.0E_44);
-42% similar to PDB:1L1Y The Crystal Structure and Catalytic Mechanism of Cellobiohydrolase CelS, the Major Enzymatic Component of the Clostridium thermocellum cellulosome (E_value = 7.0E_44);

Gene Protein Sequence:
MKKSFLLAIVMLFGIAMQGHSAPVTKERALSLARLALRQVSLRMGQTAVS
DKISIDYVYRQGDAERGITSQEEGSPAYFYVANRGNNEGYALVAADDRIP
TILAYSPIGRFDMDSMPDNLRMWLQIYDQEIGLILSGKAQLNEEILRTEG
VPAEVHALMDNGHFANDPMRWNQGYPWNNKEPLLPNGNHAYTGCVATAAA
QIMRYHSWPLQGEGSFDYHAGSLVGNWSGTFGEMYDWINMPGNPDLDNLT
QSQVDAYATLMRDVSASVSMSFYENGSGTYSVYVVGALRNNFRYKRSLQL
HVRALYTSQEWHDMIRGELASGRPVYYAGNNQSIGHAFVCDGYASDGTFH
FNWGWGGVSNGFYKLTLLSPTSLGIGGEGIGFTIYQEIITGIEPAKTPAE
AGTDALPILALKDIEAEYKSESGLNVGYSIYNTGEEQSNLDLGYRLNKAD
GEVIEVKTSSINISWYGYGEHPESFSLAPNQLSQGINTITLLYRRTGTEQ
WEPVRHAQGGYVNSIKVNTTDPNNVVVTVDNNEGKLSIVPNSFVADLNSY
EHSTITVQFNSDSPDEIRTPVAFALSTGATADDVISLGWVMAEVPGGSSN
YPVVWSKDVLTLSEGDYTLWYRFSINNQKDEWKKIGSVSVKTPTEYTHPL
FEVGHNQTSTYTLDMAHNRVLPDFTLKNLGLPFNGELVVVFRQTQSSSGS
LWAAQETVHIKQGETFVYKPVVEGPIPDGSYRATLHAFVNGQQQLYLKGK
RNYTVKIVNGTAVEAIESSEEIRVFPNPARDYVEISAPCIPQETSIILFD
LSGKIVMKNSLSAGHGRMDVSRLPNGAYILKVDGYTTKINIVH

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAAAAAAGTTTTCTTTTAGCCATAGTAATGCTCTTTGGCATTGCCAT
GCAGGGACATTCTGCTCCGGTTACGAAAGAGCGAGCTTTGAGTCTGGCTC
GGCTGGCTTTGCGACAGGTATCCTTGCGAATGGGACAAACAGCAGTATCT
GACAAGATTTCCATCGATTACGTTTATCGGCAAGGAGATGCTGAGAGGGG
TATCACATCACAAGAGGAAGGCTCTCCTGCATATTTTTATGTAGCTAATC
GTGGAAATAATGAGGGCTATGCTCTTGTAGCAGCAGATGACAGAATACCG
ACAATTTTAGCCTATTCACCCATTGGCCGTTTCGACATGGACAGTATGCC
GGACAATCTTCGCATGTGGCTACAAATTTACGATCAGGAAATAGGCCTGA
TACTTTCCGGAAAAGCTCAGCTCAATGAAGAGATATTACGTACCGAGGGC
GTACCGGCTGAAGTACATGCTCTGATGGATAACGGTCATTTTGCCAACGA
TCCCATGCGATGGAATCAAGGTTACCCATGGAACAATAAGGAACCACTGC
TTCCTAATGGCAATCATGCCTATACCGGCTGTGTTGCTACTGCTGCAGCA
CAAATCATGCGCTACCATAGCTGGCCGCTTCAAGGTGAAGGCTCTTTCGA
TTATCATGCAGGTTCATTAGTTGGCAACTGGTCCGGCACATTTGGTGAAA
TGTACGACTGGATCAATATGCCCGGAAATCCCGACCTTGATAATCTGACT
CAATCTCAAGTGGATGCCTACGCCACACTGATGCGTGATGTGAGTGCATC
TGTTTCGATGAGTTTTTATGAAAATGGAAGTGGTACGTACAGCGTTTATG
TAGTAGGAGCCTTGCGAAACAACTTTCGCTACAAGCGTTCACTGCAGCTA
CATGTACGCGCCTTATATACCTCACAGGAGTGGCACGATATGATCCGCGG
GGAACTTGCCTCCGGAAGGCCGGTCTATTATGCAGGGAATAACCAGAGCA
TAGGACATGCTTTCGTTTGCGATGGTTATGCTTCGGATGGTACTTTCCAT
TTCAACTGGGGTTGGGGAGGTGTTTCCAACGGCTTCTACAAACTAACACT
CCTCTCGCCGACTTCGTTGGGTATCGGAGGTGAGGGAATAGGTTTTACCA
TTTATCAAGAGATCATCACCGGTATCGAACCGGCTAAGACTCCCGCTGAA
GCCGGTACAGATGCCTTGCCGATCTTGGCACTGAAAGACATAGAAGCCGA
GTATAAAAGTGAATCCGGATTGAACGTAGGGTATTCGATATATAATACAG
GTGAAGAGCAATCAAATCTTGACCTCGGATACAGATTGAACAAGGCTGAC
GGAGAAGTCATAGAGGTGAAAACTTCATCTATCAATATCTCTTGGTACGG
ATACGGAGAGCATCCCGAGAGTTTCTCATTGGCACCTAATCAGTTGTCAC
AAGGAATCAACACCATCACCCTACTTTATCGTCGCACAGGCACCGAACAG
TGGGAGCCGGTACGGCATGCACAGGGAGGATATGTCAATAGCATTAAAGT
AAATACGACAGACCCGAACAATGTCGTAGTCACGGTAGATAATAACGAAG
GCAAGCTCAGTATCGTCCCCAACAGCTTTGTCGCAGATCTGAATTCTTAT
GAACATAGTACGATTACAGTACAGTTCAATAGCGACAGCCCTGATGAGAT
CCGTACACCCGTAGCCTTTGCTCTATCTACAGGAGCTACTGCGGACGATG
TAATATCTTTGGGCTGGGTAATGGCTGAAGTTCCGGGCGGTAGCAGCAAC
TATCCGGTGGTTTGGTCTAAAGACGTTCTCACTCTCTCGGAAGGCGACTA
TACATTGTGGTATAGATTTTCCATCAACAACCAAAAGGATGAATGGAAAA
AGATCGGAAGCGTGTCAGTAAAAACACCGACAGAGTATACGCACCCCTTA
TTCGAAGTGGGCCATAATCAAACTTCTACCTATACGCTGGATATGGCACA
CAACAGAGTATTGCCCGACTTTACACTCAAAAATCTCGGATTGCCTTTCA
ATGGTGAGTTGGTTGTTGTTTTCCGCCAAACACAATCCTCATCGGGGTCT
TTATGGGCAGCTCAAGAAACAGTACATATCAAGCAAGGAGAAACTTTCGT
ATATAAACCTGTTGTCGAAGGCCCTATACCTGATGGATCCTATCGTGCGA
CCCTCCATGCATTCGTAAACGGACAACAACAGTTGTACCTCAAGGGGAAA
AGGAACTACACGGTGAAGATCGTCAATGGTACAGCGGTAGAAGCAATAGA
ATCGTCAGAAGAGATCAGAGTATTCCCTAATCCGGCACGCGATTATGTGG
AAATATCGGCACCTTGCATTCCCCAAGAAACATCTATCATTCTTTTCGAT
CTGTCAGGCAAGATTGTCATGAAGAATAGTTTATCAGCGGGGCATGGCAG
AATGGATGTCAGCCGACTTCCTAATGGGGCCTACATCCTTAAGGTGGATG
GATATACGACGAAAATAAATATAGTGCAC

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