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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap tRNA-Arg-5 IGR1155 IGR1157 IGR1156 IGR1158 IGR1159 IGR1154 IGR1160 IGR1161 IGR1162 prtT, - PG1355 PG1350 folK,pppk, - PG1348 sod,sodB, - PG1354 PG1353 truB, - PG1346 bacA, - PG1345 ftsX, - PG1343 queA, - PG1347 prtC, - PG1349 prtT, - PG1356 prtT, - PG1355 PG1350 folK,pppk, - PG1348 sod,sodB, - PG1354 PG1353 truB, - PG1346 bacA, - PG1345 ftsX, - PG1343 queA, - PG1347 prtC, - PG1349 prtT, - PG1356 prtT, - PG1355 PG1350 folK,pppk, - PG1348 sod,sodB, - PG1354 PG1353 truB, - PG1346 bacA, - PG1345 ftsX, - PG1343 queA, - PG1347 prtC, - PG1349 prtT, - PG1356 PG1344 PG1344


LANL Gene ID: PG1349

GenBank Locus Tag: PG1542

DNA Molecule Name:
1  

GenBank ID:
34541202

Gene Name:
prtC  

Definition:
collagenase (hemagglutinin/protease?)

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1620931

Gene Stop:
1622172

Gene Length:
1242

Molecular Weight*:
46662

pI*:
6.40

Net Charge*:
-3.22

EC:
 

Functional Class:
Protein fate; Degradation of proteins, peptides and glycopeptides  

Gene Ontology:
Biological process
  GO:0006508    proteolysis

Molecular function
  GO:0008233    peptidase activity


Pathway: pathway table

Primary Evidence:
Kato,T., Takahashi,N. and Kuramitsu,H.K.
Sequence analysis and characterization of the Porphyromonas
gingivalis prtC gene, which expresses a novel collagenase activity.
J. Bacteriol. 174, 3889-3895 (1992)

Comment:
PG1349 is identical to the N-terminal half of AF026946. Could it extend into the putative 1350, 1351 orfs?

TIGR ID: PG1542

Proteomic Data: Proteomic Data Search
TIGR Annotationcollagenase, GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG1542-1.840.045-5.738e-07-5.738e-07
LANLPG13490.280.02
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG1349 corresponds to the previously sequenced AB006973 from GenBANK, a collagenase. GenBANK sequences Y15767, Y15766, Y15765, Y15674, Y15673, and Y15672 represent prtC sequences from other patients. PG1349 also corresponds to the first 417 amino acids of AF026946, a predicted hagE protein. See also AB006973 and M60404.

Numerous significant hits in gapped BLAST; e.g. residues 7-414 are 36% similar to gb|AAC07088.1| collagenase of Aquifex aeolicus, residues 7-414 are 36% similar to gb|AAB85150.1| collagenase of Methanothermobacter thermautotrophicus, residues 7-407 are 36% similar to gb|AAB98071.1| collagenase (prtC) of Methanococcus jannaschii.

This sequence is similar to BT3072.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34541202|ref|NP_905681.1|  collagenase [Porphyromonas gin...   840   0.0  
 gi|2505967|dbj|BAA22606.1|  collagenase [Porphyromonas gingi...   836   0.0  
 gi|38605651|sp|P59916|PRTC1_PORGI  Collagenase precursor >gi...   684   0.0  
 gi|4138325|emb|CAA75766.1|  collagenase [Porphyromonas gingi...   681   0.0  
 gi|4138327|emb|CAA75767.1|  collagenase [Porphyromonas gingi...   681   0.0  
 gi|4138329|emb|CAA75768.1|  collagenase [Porphyromonas gingi...   680   0.0  
 gi|4138323|emb|CAA75765.1|  collagenase [Porphyromonas gingi...   679   0.0  
 gi|4138321|emb|CAA75764.1|  collagenase [Porphyromonas gingi...   678   0.0  
 gi|4138331|emb|CAA75769.1|  collagenase [Porphyromonas gingi...   673   0.0  
 gi|154489879|ref|ZP_02030140.1|  hypothetical protein PARMER...   649   0.0


InterPro Summary:  InterProScan

InterPro
IPR001539
Family
Peptidase U32
PD004398 [120-286]T 7e-95 PD004398 Q9R756_PORGI_Q9R756; Q9R756_PORGI_Q9R756;
PF01136 [78-314]T 1.7e-72 PF01136 Peptidase_U32 Peptidase_U32
PS01276 [172-190]T 8e-5 PS01276 PEPTIDASE_U32 PEPTIDASE_U32


COGS Summary:  COGS Search
BeTs to 6 clades of COG0826
COG name: Protease related to collagenase
Functional Class: O
The phylogenetic pattern of COG0826 is -MT--q--EB-huj-------
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
***** IPB001539 (Peptidase family U32) with a combined E-value of 1.5e-59.
    IPB001539A    7-32
    IPB001539B    171-208
    IPB001539C    251-276


 ProDom Summary:  Protein Domain Search
Residues 321-414 are 87% similar to a (COLLAGENASE HYDROLASE PROTEASE PRECURSOR) protein domain (PD014101 which is seen in O24788_PORGI.

Residues 7-308 are 93% similar to a (PROTEASE PROTEIN HYDROLASE PUTATIVE) protein domain (PD004398 which is seen in O24788_PORGI.


Paralogs:  Local Blast Search
Residues 7-312 show 33% similarity to PG0679, a protease.

Pfam Summary:  Pfam Search
Residues 78 to 314 (E-value = 1.5e-72) place PG1349 in the Peptidase_U32 family which is described as Peptidase family U32 (PF01136)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MNVNDFEIMAPVGSYESLMAAIKAGADSVYFGIEGLNMRARSANNFTTED
LYKIAEICRDKGVKSYLTVNTVIYDEDIALMRSVIDAAQKAQISAIIASD
VAAMMYANEIGVEVHLSTQLNISNAEALRFYSRFADVVVLARELNMDQVR
TIHETIVRDNICGPKGHPVRIEMFAHGALCMAVSGKCYLSLHEHNSSANR
GACAQICRRGYTVKDKDSGLELDIENQYIMSPKDLKTIHFINKMMDAGVR
VFKIEGRARGPEYVYTVCRCYKEAIEAYCNGTYDEEAIGRWDEQLATVFN
RGFWDGYYLGQRLGEWTHRYGSGATRQKIYVGKGIKYFSRLGVAEFEIES
GELHIGDEIVITGPTTGVIIQKVEEIRYELQTVEKATKGQRISIPVKEKV
RPSDKLYRFDKREE

Gene Nucleotide Sequence:  Sequence Viewer
ATGAATGTAAACGACTTCGAGATAATGGCTCCAGTCGGTTCGTACGAATC
GCTTATGGCAGCCATCAAGGCAGGAGCAGATTCAGTTTACTTCGGGATTG
AAGGACTGAATATGCGTGCGCGATCTGCCAACAACTTCACCACAGAAGAT
CTGTACAAAATAGCCGAGATTTGCAGAGATAAAGGCGTAAAGAGCTATTT
AACGGTGAATACCGTCATATACGATGAGGACATAGCACTCATGCGCTCCG
TCATCGATGCGGCACAAAAGGCACAAATATCTGCCATTATAGCTTCCGAC
GTAGCTGCGATGATGTATGCCAACGAGATCGGAGTAGAAGTGCATCTGTC
CACTCAGCTCAATATCAGCAACGCGGAGGCCCTACGCTTTTATTCGCGCT
TTGCCGATGTGGTCGTATTGGCAAGAGAGCTGAATATGGATCAGGTGCGT
ACAATCCACGAGACCATCGTCAGGGATAATATCTGTGGGCCTAAAGGCCA
TCCCGTACGTATAGAGATGTTTGCTCACGGCGCTCTGTGTATGGCCGTTT
CGGGCAAGTGCTATCTAAGCCTGCACGAACACAACAGCTCCGCCAACAGA
GGAGCCTGTGCGCAGATCTGCAGGAGGGGCTACACCGTCAAGGACAAGGA
TAGCGGTCTGGAACTGGACATTGAGAACCAATACATCATGTCGCCGAAAG
ATCTGAAGACTATTCATTTCATCAATAAGATGATGGATGCCGGCGTACGA
GTATTCAAGATAGAAGGAAGGGCACGTGGCCCCGAATACGTCTATACGGT
CTGCCGCTGCTATAAAGAAGCGATCGAAGCCTACTGCAACGGCACCTATG
ATGAAGAGGCCATAGGCCGGTGGGACGAACAATTGGCTACGGTATTCAAC
CGAGGCTTTTGGGATGGCTACTACCTCGGACAACGGCTCGGCGAATGGAC
ACATCGTTACGGCTCAGGAGCTACGCGACAGAAAATATATGTAGGCAAGG
GGATCAAATACTTCAGCCGTCTCGGTGTGGCTGAATTCGAGATAGAGTCC
GGCGAACTGCATATAGGCGATGAGATTGTGATCACCGGCCCTACTACAGG
TGTGATCATCCAAAAGGTGGAAGAGATCCGATACGAACTGCAAACCGTGG
AAAAGGCGACAAAGGGACAACGCATTTCCATTCCGGTAAAGGAGAAAGTG
CGTCCGTCGGACAAGCTCTACCGGTTCGACAAAAGAGAAGAA

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