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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap tRNA-Arg-5 IGR1164 IGR1165 IGR1158 IGR1159 IGR1160 IGR1161 IGR1162 IGR1163 prtT, - PG1355 PG1350 hmuY, - PG1357 sod,sodB, - PG1354 PG1353 prtC, - PG1349 hmuR, - PG1358 prtT, - PG1356 cobN, - PG1359 prtT, - PG1355 PG1350 hmuY, - PG1357 sod,sodB, - PG1354 PG1353 prtC, - PG1349 hmuR, - PG1358 prtT, - PG1356 cobN, - PG1359 prtT, - PG1355 PG1350 hmuY, - PG1357 sod,sodB, - PG1354 PG1353 prtC, - PG1349 hmuR, - PG1358 prtT, - PG1356 cobN, - PG1359


LANL Gene ID: PG1356

GenBank Locus Tag: PG1548

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
prtT  

Definition:
protease/hemagglutinin PrtT

Cellular Location:
Cytoplasm, Extracellular [Evidence]

Gene Start:
1624984

Gene Stop:
1627503

Gene Length:
2520

Molecular Weight*:
93416

pI*:
5.30

Net Charge*:
-21.18

EC:
3.4.22.-  

Functional Class:
Protein fate; Degradation of proteins, peptides and glycopeptides  

Gene Ontology:
Biological process
  GO:0006508    proteolysis

Molecular function
  GO:0008234    cysteine-type peptidase activity


Pathway: pathway table

Primary Evidence:
Madden,T.E., Clark,V.L. and Kuramitsu,H.K.
Revised sequence of the Porphyromonas gingivalis prtT cysteine
protease/hemagglutinin gene: homology with streptococcal pyrogenic exotoxin B/streptococcal proteinase.
Infect. Immun. 63 (1), 238-247 (1995)

Kuramitsu,H.K.
Isolation and characterization of the Porphyromonas gingivalis prtT gene, coding for protease activity
Infect. Immun. 61, 117-123 (1993)

Otogoto,J. and Kuramitsu,H.K.
Isolation and characterization of the Porphyromonas gingivalis prtT gene, coding for protease activity
Infect. Immun. 61 (1), 117-123 (1993)



Comment:
TIGR ID PG0990

Proteomic Data: Proteomic Data Search
TIGR Annotationthiol protease/hemagglutinin PrtT precursor, authentic frameshift, GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG1548-1.000.0018-1.581.9e-05-1.111.7e-05-1.581.9e-05
LANLPG13560.500.330.460.22
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
This sequence corresponds to the previously sequenced S75942 in GenBANK, a predicted 99 kDa cysteine protease/hemagglutinin in
P.gingivalis, strain ATCC 53977.

Numerous significant hits to pyrogenic exotoxin B of Streptococcus pyogenes in gapped BLAST; e.g. residues 8-385 are 31% similar to gb|AAA26980.1| pyrogenic exotoxin B of Streptococcus pyogenes.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|913137|gb|AAB32891.1|  PrtT [Porphyromonas gingivalis]        1645   0.0  
 gi|1172653|sp|P43158|PRTT_PORGI  Thiol protease/hemagglutini...  1642   0.0  
 gi|1314753|gb|AAC44980.1|  hemin-regulated protein                508   e-142
 gi|34541102|ref|NP_905581.1|  thiol protease/hemagglutinin P...   467   e-129
 gi|431616|gb|AAA26980.1|  pyrogenic exotoxin B [Streptococcu...   190   5e-46
 gi|55669548|pdb|1PVJ|A  Chain A, Crystal Structure Of The St...   188   2e-45
 gi|431656|gb|AAA27000.1|  pyrogenic exotoxin B [Streptococcu...   182   7e-44
 gi|94995224|ref|YP_603322.1|  Streptopain precursor [Strepto...   182   7e-44
 gi|431666|gb|AAA27005.1|  pyrogenic exotoxin B [Streptococcu...   182   1e-43
 gi|431646|gb|AAA26995.1|  pyrogenic exotoxin B [Streptococcu...   181   1e-43


InterPro Summary:  InterProScan

InterPro
IPR000200
Family
Peptidase C10, streptopain
PD004169 [156-385]T 3e-138 PD004169 Q53481_PORGI_Q53481; Q53481_PORGI_Q53481;
PR00797 [177-201]T 2.4e-44 PR00797 STREPTOPAIN[247-263]T 2.4e-44 PR00797 STREPTOPAIN[275-294]T 2.4e-44 PR00797 STREPTOPAIN[308-332]T 2.4e-44 PR00797 STREPTOPAIN[340-361]T 2.4e-44 PR00797 STREPTOPAIN[362-383]T 2.4e-44 PR00797 STREPTOPAIN STREPTOPAIN
PF01640 [4-385]T 4.1e-320 PF01640 Peptidase_C10 Peptidase_C10
noIPR
unintegrated
unintegrated
signalp [1-20]? NA signalp signal-peptide signal-peptide


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
No significant hit to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 602-851 are 98% similar to a (HEMIN-REGULATED PROTEIN THIOL PROTEASE/HEMAGGLUTININ) protein domain (PD039116 which is seen in P72198_PORGI.

Residues 151-430 are 99% similar to a (EXOTOXIN B PYROGENIC PYROGEN) protein domain (PD004169 which is seen in PRTT_PORGI.

Residues 46-150 are 88% similar to a (THIOL PROTEASE/HEMAGGLUTININ PRTT) protein domain (PD133794 which is seen in PRTT_PORGI.

Residues 431-601 are 98% similar to a (THIOL PROTEASE/HEMAGGLUTININ PRTT) protein domain (PD133795 which is seen in PRTT_PORGI.


Paralogs:  Local Blast Search
PG1356 is similar to PG1251, a predicted thiol protease (PrtT related). Residues 46-884 are 34% similar to residues 1-841 of PG1251. Also, weak similarity to PG1208, immunoreactive 47 kDa antigen PG97, and PG1838, an immunoreactive antigen.

Pfam Summary:  Pfam Search
No significant hits to the Pfam 11.0 database

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  
uncleavable signal  
1  
44
transmembrane  
5  
21
transmembrane  
49  
65

Top PDB Hits:
-50% similar to PDB:1PVJ Crystal structure of the Streptococcal pyrogenic exotoxin B (SpeB)- inhibitor complex (E_value = 3.6E_48);
-49% similar to PDB:1DKI CRYSTAL STRUCTURE OF THE ZYMOGEN FORM OF STREPTOCOCCAL PYROGENIC EXOTOXIN B ACTIVE SITE (C47S) MUTANT (E_value = 1.4E_47);
-40% similar to PDB:2I0Z Crystal structure of a FAD binding protein from Bacillus cereus, a putative NAD(FAD)-utilizing dehydrogenases (E_value = 1.4E_47);
-38% similar to PDB:1W5D CRYSTAL STRUCTURE OF PBP4A FROM BACILLUS SUBTILIS (E_value = 1.4E_47);
-40% similar to PDB:1D3A CRYSTAL STRUCTURE OF THE WILD TYPE HALOPHILIC MALATE DEHYDROGENASE IN THE APO FORM (E_value = 1.4E_47);

Gene Protein Sequence:
MKRIFYTLGLLLLCLPMLQAGPVTRSKAEQTAKNFFAKRQPTLSSSTASL
RMDFVYKAAEREEALFFVFNRGEKDGFLLVAADDRFPEVIGYAFKGHFDA
ARIPDNLRGWLKGYEREMLAVMDGKAEPIDPIREAKPTRDLPSSIAPILE
TGEHASDPILWDQGYPFNTLHPLLPSGQQAYTGCVATAMGQIMRHYKWPE
KASGEYDYYDDMTGTHTHYSGTFGETYNWSKMPGNISVGISPEEVKALST
FMRDVSFSVNMQFADFGSGTFSIFVERALRETFHYKKSLRYIHRSLLPGK
EWKDMIRKELAENRPVYYAGADGSMGHAFVCDGYEPDGTFHFNWGWGGMS
NGNFYLNLLNPGSLGTRAGDGGYSTDQEVVIGIEPASNEVPGIVPDPTIT
LYGLQHNMSDEALDLSVKIKNYSTYAGDVKLAYRLTLPNGTETTNPAVTV
PIVWEDIIGESTGNITIPCSQFAEGKNTISILYRTDGMADWKELKHILMG
LVNKIEVTMPAGDVAYSVADARIVLKDGSLSHDLKAYSDCKLSATVYNPG
TEEFRSRVTFALRNTEGRLYFLGRHLVELHPGDEDGEKVSLTITGLKARA
GQYMLVCTGDMESLMEDASWIELASIEVAEHTSTHSSLLVASNPQIDLLT
VHRANPETLPTFSITNEGGATFSGKIEIVAIKAFSETFFQAKEEHMSLAQ
GETKVLSPELTANSSLYTNAELFPDGTYYIVIREQGFWDPIDLFGDYYYR
IRLITDLSSSDIAGKDVSTIVLYPNPAHDYVHVAIPPTYAGSTLRLFDIQ
GRMQLSTKIESADMRLDVERLPKGTYIVVVEDMVGKLFIR

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAACGTATCTTCTACACCTTAGGGCTATTATTACTGTGTCTCCCTAT
GCTCCAGGCAGGACCGGTGACACGATCCAAAGCCGAACAGACGGCTAAGA
ACTTTTTTGCCAAACGACAACCCACGCTGTCTTCATCGACAGCGAGTCTC
CGGATGGATTTCGTTTACAAAGCTGCAGAAAGAGAGGAGGCACTATTCTT
CGTTTTCAATCGAGGAGAGAAAGACGGATTTCTCCTCGTCGCAGCGGATG
ATCGGTTCCCGGAGGTGATCGGATATGCTTTCAAGGGGCACTTCGATGCG
GCCCGTATACCGGACAATCTCAGAGGGTGGCTCAAAGGTTATGAACGTGA
AATGCTTGCTGTAATGGACGGCAAGGCAGAGCCGATAGATCCTATCCGTG
AAGCCAAGCCTACACGGGACCTGCCATCATCCATTGCCCCTATTTTGGAA
ACGGGCGAACATGCATCGGATCCGATCTTGTGGGATCAGGGCTATCCATT
TAACACCTTGCATCCCCTGCTTCCTTCCGGGCAGCAGGCTTATACCGGTT
GTGTTGCCACTGCCATGGGACAAATCATGCGTCACTACAAGTGGCCGGAG
AAGGCTTCCGGCGAATATGACTACTACGATGATATGACGGGGACGCATAC
CCACTATTCAGGCACATTCGGTGAGACCTATAACTGGAGCAAGATGCCCG
GTAACATCAGTGTTGGTATCAGCCCTGAAGAAGTAAAAGCCTTAAGTACT
TTCATGAGAGATGTCAGCTTTTCTGTCAATATGCAGTTTGCCGATTTCGG
CAGTGGCACTTTCTCCATATTTGTCGAACGCGCCCTGCGGGAGACCTTTC
ATTACAAGAAATCGCTTCGTTACATCCATCGCTCTCTCCTTCCCGGTAAG
GAATGGAAAGACATGATTCGTAAGGAACTGGCAGAAAACAGACCTGTGTA
TTATGCCGGTGCAGACGGATCGATGGGACATGCTTTCGTTTGCGATGGAT
ACGAACCAGACGGAACGTTCCACTTCAACTGGGGATGGGGTGGCATGAGT
AACGGTAATTTCTATCTCAATCTACTCAATCCGGGTTCGCTTGGCACACG
CGCAGGCGATGGAGGCTACTCTACTGACCAAGAGGTTGTCATAGGCATAG
AGCCGGCCAGCAATGAAGTCCCCGGTATCGTACCGGATCCGACCATCACG
CTCTATGGATTGCAACACAATATGTCTGATGAAGCACTTGACCTGAGTGT
AAAAATCAAGAACTACTCCACATATGCAGGGGATGTGAAATTGGCTTATC
GCCTGACGCTTCCCAATGGAACGGAAACGACTAACCCAGCCGTCACCGTC
CCTATCGTTTGGGAAGACATTATCGGAGAAAGCACGGGCAACATAACCAT
ACCATGCAGTCAATTTGCCGAGGGGAAAAATACCATCTCCATACTCTATC
GAACAGATGGCATGGCAGATTGGAAAGAGCTTAAGCACATACTTATGGGG
CTTGTCAATAAGATAGAAGTGACTATGCCTGCCGGAGATGTAGCTTACTC
CGTTGCCGATGCCAGGATCGTTCTCAAAGACGGTTCTCTTTCGCACGACC
TGAAAGCATATTCCGACTGCAAACTCAGTGCCACAGTCTACAACCCGGGT
ACGGAAGAATTTCGTAGTCGCGTCACCTTTGCCCTCCGCAATACAGAGGG
ACGGCTCTATTTCCTTGGCAGACATTTAGTAGAATTACACCCGGGAGATG
AAGATGGCGAAAAAGTTTCGCTGACAATAACAGGGCTCAAGGCTCGTGCA
GGACAATACATGCTTGTCTGTACGGGCGATATGGAATCGCTTATGGAAGA
TGCCTCATGGATAGAGTTGGCTTCCATAGAAGTAGCAGAGCATACGAGCA
CCCATTCATCGTTACTGGTGGCCTCCAACCCACAGATCGATCTTCTCACA
GTTCATCGGGCCAATCCCGAGACATTGCCGACTTTCAGCATTACAAATGA
AGGTGGTGCTACTTTCTCCGGGAAAATCGAAATAGTGGCTATAAAGGCTT
TCTCGGAAACTTTCTTCCAAGCGAAAGAAGAACACATGAGTCTCGCCCAA
GGGGAAACCAAAGTATTGTCTCCGGAGCTGACTGCGAACTCTTCTCTCTA
TACAAATGCCGAACTCTTTCCCGATGGCACCTATTACATTGTCATCAGAG
AGCAGGGATTTTGGGATCCGATCGATTTGTTTGGGGACTATTACTATCGT
ATCCGTCTCATTACGGATCTATCCTCTTCGGACATCGCTGGTAAGGATGT
TTCTACTATAGTACTTTATCCCAATCCTGCTCACGACTATGTCCATGTAG
CCATTCCTCCCACATATGCGGGCAGCACACTTCGTTTGTTCGATATTCAA
GGGCGAATGCAGCTCTCCACGAAGATCGAATCTGCCGATATGCGTCTCGA
CGTCGAACGTCTTCCGAAGGGCACCTATATCGTTGTGGTAGAAGACATGG
TAGGGAAGCTCTTTATTCGT

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