LANL Gene ID: PG1422
GenBank Locus Tag: PG1624
DNA Molecule Name:
1
GenBank ID:
34541274
Gene Name:
ISPg1
Definition:
ISPg1 transposase (IS1106-like transposase)
Cellular Location:
Cytoplasm [Evidence]
Gene Start:
1707366
Gene Stop:
1706284
Gene Length:
1083
Molecular Weight*:
41742
pI*:
10.20
Net Charge*:
14.72
EC:
Functional Class:
Transposon functions
Gene Ontology:
Biological process
GO:0006313 transposition, DNA-mediated
Molecular function
GO:0003677 DNA binding
GO:0004803 transposase activity
Pathway: pathway table
Primary Evidence:
Hanley,S.A., Aduse-Opoku,J. and Curtis,M.A.
A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis
W50 has arisen via horizontal gene transfer
Infect. Immun. 67 (3), 1157-1171 (1999)
Maley,J. and Roberts,I.S.
Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements
Microbiol. Lett. 123 (1-2), 219-224 (1994)
Shibata,Y., Hayakawa,M., Takiguchi,H., Shiroza,T. and Abiko,Y.
Determination and characterization of the hemagglutinin-associated
short motifs found in Porphyromonas gingivalis multiple gene
products. J. Biol. Chem. 274 (8), 5012-5020 (1999)
Secondary Evidence:
Knight,A.I., Ni,H., Cartwright,K.A. and McFadden,J.J.
Identification and characterization of a novel insertion sequence,
IS1106, downstream of the porA gene in B15 Neisseria meningitidis.
Mol. Microbiol. 6 (11), 1565-1573 (1992)
Comment:
Member of the IS5 family of elements. A full-length ISPg1 element.
TIGR ID: PG1624
Human Oral Microbiome Database:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
PG1422 is equivalent to the previously sequenced AJ130872 and AB015879 in GenBANK. Residues 31-350 are 34% similar to the IS1106 transposase of N. meningitidis (AE002488). Residues 1-156 and 282-361 conform to the previously sequenced IS1126 element of gingivalis (AB019363).
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
InterPro Summary: InterProScan
| InterPro IPR002559 Domain 
|
| |||||||
![[147-348]T 2.9000035467795803E-37 PF01609 Transposase_11](http://www.ebi.ac.uk/InterProScan/images/H.gif){kind=small}
COGS Summary: COGS Search
No hit to the COGs database.
Blocks Summary: Blocks Search
No significant hit to the Blocks database.
ProDom Summary: Protein Domain Search
Residues 157-302 are 87% similar to a (TRANSPOSASE PLASMID PROTEIN ELEMENT) protein domain (PD004652 which is seen in Q9ZA61_PORGI.
Residues 12-109 are identical to a (TRANSPOSASE PROTEIN INSERTION ELEMENT) protein domain (PD007264 which is seen in Q9ZAD0_PORGI.
Residues 303-361 are identical to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187845 which is seen in Q9ZA61_PORGI.
Residues 110-156 are identical to a (TRANSPOSASE ORF7 INSERTION SEQUENCE IS1126-LIKE GENE) protein domain (PD187846 which is seen in Q9ZA61_PORGI.
Paralogs: Local Blast Search
PG1422 is virtually identical to PG1267, PG0169, PG1050, PG1062, PG0740, PG0918, PG1662, PG0420, PG0499, and PG1099. Similarities exist to PG0732, PG0842, PG1607, PG0731, PG0884, PG1184, PG1087, PG0763, PG1755, PG0686, PG0756, PG1798, PG1230, PG1919, PG0044, PG0689, PG1215, PG1665, PG0839, PG1895, PG0883, PG1231, PG0730, PG1159 and PG1608.
Pfam Summary: Pfam Search
Residues 147 to 348 (E-value = 2.2e-40) place PG1422 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)
Structural Feature(s):
| Feature Type | Start | Stop |
| transmembrane | ||
| non-globular | ||
| coil-coil |
Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).
Gene Protein Sequence:
MAYQSKNTDEHVTFADALLSKRYRKAQNDFLNQVDRLIDWRPIRTLINKK
YTKRQNAIGAPAYDVILLFKMLLLETWYNLSDCALEERINDSITFSRFLG
LKMEEVSPDHSTISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLV
DASLVETPHKPNGSITIEVADDREDNRSEEEKEAEEDYQKQVVRQRKGTD
EEARWVYKQKRYHYGYKKHCLTNVQGIVQKVITTAANRSDTKEFIALLQG
ANIPQGSAVLADKGYACGENRSYLQTHHLQDGIMHKAQRNRALTEEEKQR
NKAISRIRSTIERTFGSIRRWFHGGRCRYRGLAKTHTQNILESIAFNLYR
TPGIIMSSFVG
Gene Nucleotide Sequence: Sequence Viewer
ATGGCATACCAATCCAAGAATACCGATGAGCATGTAACATTTGCAGACGC
ACTCCTTTCAAAGCGTTATCGCAAAGCACAAAACGACTTCCTCAATCAGG
TTGACAGGCTTATCGATTGGCGTCCGATCAGGACGCTGATCAACAAGAAA
TACACCAAGCGACAAAATGCCATCGGCGCCCCGGCTTATGACGTGATTCT
CTTATTCAAGATGTTGCTTTTGGAGACATGGTACAACCTCAGTGATTGTG
CTTTGGAGGAGCGCATCAATGATTCAATCACCTTTTCCCGATTCTTGGGG
CTGAAGATGGAAGAGGTTTCTCCCGACCACAGTACCATCAGTCGATTTCG
TTCGGCACTGACAGAGTTGGGTCTCATGGACAAACTATTGGCGCAGTTTA
ACAAACAACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTC
GATGCAAGCCTTGTGGAGACGCCGCATAAACCCAACGGAAGCATTACGAT
TGAAGTCGCAGACGACAGAGAAGACAATCGGAGCGAGGAGGAAAAAGAGG
CAGAGGAGGATTATCAAAAACAGGTTGTCCGTCAGCGTAAAGGGACGGAT
GAAGAAGCCCGTTGGGTTTACAAACAAAAGCGTTATCACTACGGATACAA
AAAGCATTGTCTGACCAATGTTCAAGGCATTGTTCAAAAGGTGATAACGA
CTGCAGCGAACCGCAGTGACACGAAGGAGTTTATTGCGCTATTGCAGGGT
GCAAACATACCTCAAGGCTCAGCCGTCTTGGCGGACAAAGGATATGCTTG
CGGGGAAAATCGTTCCTACCTGCAAACCCATCACCTTCAAGACGGCATCA
TGCACAAGGCACAACGCAACAGGGCATTGACCGAGGAAGAGAAGCAACGA
AACAAAGCAATCAGTCGGATACGGAGCACCATCGAACGCACCTTTGGCAG
TATTCGCCGGTGGTTTCATGGCGGACGATGTCGATACCGGGGACTTGCCA
AGACCCATACTCAAAACATTCTTGAAAGCATCGCCTTTAATTTATACAGA
ACCCCGGGGATAATTATGTCCTCATTTGTAGGA