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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1227 IGR1226 IGR1228 IGR1230 IGR1232 IGR1231 IGR1233 IGR1229 PG1435 ISPg5, - PG1437 ltp1,ptpA, - PG1433 ISPg5, - PG1436 dinF, - PG1432 zntA, - PG1434 cls, - PG1438 relA, - PG1440 PG1435 ISPg5, - PG1437 ltp1,ptpA, - PG1433 ISPg5, - PG1436 dinF, - PG1432 zntA, - PG1434 cls, - PG1438 relA, - PG1440 Type: IS element, Name: ISPg5 - 8 PG1435 ISPg5, - PG1437 ltp1,ptpA, - PG1433 ISPg5, - PG1436 dinF, - PG1432 zntA, - PG1434 cls, - PG1438 relA, - PG1440


LANL Gene ID: PG1436

GenBank Locus Tag: PG1644

DNA Molecule Name:
1  

GenBank ID:
34541287

Gene Name:
ISPg5  

Definition:
ISPg5 transposase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1725712

Gene Stop:
1724777

Gene Length:
936

Molecular Weight*:
36374

pI*:
10.20

Net Charge*:
15.52

EC:
 

Functional Class:
Transposon functions  

Gene Ontology:
Biological process
  GO:0015074    DNA integration

Molecular function
  GO:0003677    DNA binding


Pathway: pathway table

Primary Evidence:
Califano,J.V., Kitten,T., Lewis,J.P., Macrina,F.L., Fleischmann,R.D., Fraser,C.M.,Duncan,M.J. and Dewhirst,F.E. Characterization of Porphyromonas gingivalis insertion
sequence-like element ISPg5.
Infect. Immun. 68 (9), 5247-5253 (2000)

Comment:
One of eleven ISPg5 elements in the genome (see Paralogs). Califano et al. report considerable strain diversity of these (Infect. Immun. 68 (9), 5247-5253 (2000)).

Member of the IS3 family of insertion elements. See PG1437 for associated ORF.

TIGR ID: PG1644

Human Oral Microbiome Database:
View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
PG1436 is equivalent to the previously reported gb|AAF69128.1| in GenBANK.

Numerous significant hits in gapped BLAST to transposase proteins; e.g. residues 91-213 are 55% similar to transposase homolog (U67062) of Bacteriodes thetaiotaomicrom, residues 5-268 are 36% similar to (AE004463) hypothetical protein of Pseudomonas aeruginosa, residues 64-287 are 32% similar to InsB (AF153317) of Shigella dysenteriae, residues 64-268 are 33% similar to Hp1 (AF081284) of E.coli.

Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34541287|ref|NP_905766.1|  ISPg5, transposase Orf2 [Porph...   625   e-178
 gi|34539915|ref|NP_904394.1|  ISPg5 transposase Orf2 [Porphy...   619   e-176
 gi|34540277|ref|NP_904756.1|  ISPg5, transposase Orf2 [Porph...   616   e-175
 gi|34540399|ref|NP_904878.1|  ISPg5, transposase Orf2 [Porph...   616   e-175
 gi|34541341|ref|NP_905820.1|  ISPg5, transposase Orf2 [Porph...   615   e-175
 gi|34541641|ref|NP_906120.1|  ISPg5, transposase Orf2 [Porph...   613   e-174
 gi|34540248|ref|NP_904727.1|  ISPg5, transposase Orf2 [Porph...   611   e-173
 gi|34539888|ref|NP_904367.1|  ISPg5 transposase Orf2 [Porphy...   610   e-173
 gi|118200270|dbj|BAF37077.1|  transposase Orf2 [Porphyromona...   609   e-173
 gi|34540696|ref|NP_905175.1|  ISPg5, transposase Orf2 [Porph...   607   e-172


InterPro Summary:  InterProScan

InterPro
IPR001584
Domain
Integrase, catalytic core
PF00665 [110-273]T 1e-31 PF00665 rve rve
PS50994 [110-276]T 18.951 PS50994 INTEGRASE INTEGRASE
noIPR
unintegrated
unintegrated
G3DSA:3.30.420.10 [113-281]T 4.5e-07 G3DSA:3.30.420.10 no description no description


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01836 (Transposase) with a combined E-value of 2.2e-21.
    PF01836B    103-154
    PF01836C    181-190
    PF01836D    215-222
    PF01836E    253-272


 ProDom Summary:  Protein Domain Search
No significant hit to the ProDom database.

Paralogs:  Local Blast Search
PG1436 is paralogous to PG1861, PG1491, PG0391, PG0418, PG0007, PG1796, PG0034, PG0533, PG1247 and PG0841, all ISPg5 transposases.

Pfam Summary:  Pfam Search
Residues 110 to 273 (E-value = 8.9e-31) place PG1436 in the rve family which is described as Integrase core domain (PF00665)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
LLGFSRQAFYKRHLNDLAQHEEDVLCSSIIQYCWHLRQAEHLPQAGFREL
MVLCQQYFGPKFTLGRDRFCALLRRHGMMLRKRSVRPRTTNSRHRLYKYE
DLLNTEPKFVPQRPGELLVADITYVAYQDGFAYLSLLTDAYSRCIVGYCL
HPTLEVEGCLNALHQAFAFYDQHQIDTTNMIHHSDRGIQYAGKSYTDLLH
GRGWRISMTQTGDPQHNALAERMNNTLKNSWHISSSKQSFDQALLSVEQA
VRMYNEARPHQALRAKTPMQVIAPESKNPLLTRREHGPEIAPELYRRMNV
RQRANFARVNRN

Gene Nucleotide Sequence:  Sequence Viewer
CTGCTTGGCTTTAGCCGTCAGGCCTTTTATAAACGTCATCTCAACGATTT
AGCACAACATGAGGAGGATGTCCTATGCAGTAGTATCATCCAATATTGTT
GGCACTTAAGACAAGCAGAGCACCTACCTCAAGCCGGCTTCCGAGAGCTC
ATGGTGCTTTGTCAGCAATACTTCGGCCCCAAGTTCACGCTTGGGCGCGA
TCGCTTTTGCGCCCTACTCAGACGCCATGGTATGATGCTGCGCAAACGGT
CTGTGCGCCCTCGGACAACCAACTCTCGGCATAGGCTCTACAAGTATGAA
GACCTGCTCAATACAGAGCCTAAGTTTGTGCCTCAAAGACCTGGGGAACT
CCTTGTAGCAGACATCACCTACGTGGCTTACCAAGATGGGTTTGCCTATC
TCTCCCTACTGACCGATGCCTATAGTCGCTGTATCGTAGGGTATTGCTTA
CATCCGACCCTGGAAGTTGAAGGCTGTTTGAATGCTCTGCATCAAGCCTT
TGCTTTCTACGATCAACATCAAATTGATACGACCAATATGATTCATCATA
GCGACCGAGGCATTCAGTATGCCGGTAAGAGTTACACCGATCTGTTGCAT
GGGCGAGGCTGGCGCATCAGTATGACTCAGACGGGAGATCCTCAGCATAA
TGCATTGGCCGAGCGGATGAACAATACGCTCAAGAACTCGTGGCACATCT
CCTCTTCGAAGCAGTCTTTTGATCAAGCACTCCTTTCTGTAGAACAAGCC
GTGCGCATGTACAACGAGGCGCGTCCACATCAAGCTCTAAGGGCGAAAAC
GCCTATGCAAGTCATTGCGCCAGAGTCTAAAAATCCGTTACTAACGAGGA
GGGAGCATGGGCCAGAGATTGCCCCCGAGCTATACCGAAGGATGAATGTC
AGACAAAGAGCTAACTTTGCTCGTGTCAACCGAAAT

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