LANL Gene ID: PG1447
GenBank Locus Tag: PG1658
DNA Molecule Name:
1
Genomic Island ID:
26
GenBank ID:
34541299
Gene Name:
ISPg4
Definition:
ISPg4 transposase
Cellular Location:
Inner membrane, Cytoplasm [Evidence]
Gene Start:
1742997
Gene Stop:
1741843
Gene Length:
1155
Molecular Weight*:
44087
pI*:
10.20
Net Charge*:
16.13
EC:
Functional Class:
Transposon functions
Gene Ontology:
Biological process
GO:0006313 transposition, DNA-mediated
Molecular function
GO:0003677 DNA binding
GO:0004803 transposase activity
Pathway: pathway table
Primary Evidence:
Sawada,K., Kokeguchi,S., Hongyo,H., Sawada,S., Miyamoto,M.,
Maeda,H., Nishimura,F., Takashiba,S. and Murayama,Y.
Identification by subtractive hybridization of a novel insertion
sequence specific for virulent strains of porphyromonas gingivalis. Infect. Immun. 67 (11), 5621-5625 (1999)
Secondary Evidence:
Nagai,T., Phan Tran,L.S., Inatsu,Y. and Itoh,Y.
A new IS4 family insertion sequence, IS4Bsu1, responsible for
genetic instability of poly-gamma-glutamic acid production in
bacillus subtilis. J. Bacteriol. 182 (9), 2387-2392 (2000). Xu,K., He,Z.Q., Mao,Y.M., Sheng,R.Q. and Sheng,Z.J. On two transposable elements from Bacillus stearothermophilus. Plasmid 29 (1), 1-9 (1993). PubMed: 8382825.
Comment:
Member of the IS5 family of IS elements.
From PubMed: 8382825:
Transposable elements were identified in the thermophile Bacillus stearothermophilus CU21 based upon the following criteria: (1) both were found to appear on different plasmids introduced into the same host CU21; (2) signals of homology were found between the genomic DNA of CU21 and each of them; (3) different numbers of Southern hybridization bands were found for the genomic DNA of different strains of B. stearothermophilus; and (4) characteristic inverted repeats at both ends and direct repeats of the target DNA adjacent to them were found for both IS5376 and IS5377. Two open reading frames (ORFs) were detected for IS5376 and one for IS5377. The putative coding products of the ORFs are homologous to those of known ISs from mesophiles and are considered to be transposases.
TIGR ID: PG1658
Human Oral Microbiome Database:
View in HOMD Genome Viewer
Blast Summary: PSI-Blast Search
PG1447 is equivalent to the previously sequenced AF148127 in GenBANK. See also AB009361, AB003149. Several moderate hits in gapped BLAST to transposases. Residues 78-336 are 28% similar to (AE000108) of Rhizobium sp. Residues 20-337 are 27% similar to a transposase from B.subtilis (AB031551). Residues 3-340 are 26% similar to (AP001509) of Bacillus halodurans.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
InterPro Summary: InterProScan
| InterPro IPR002559 Domain 
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| InterPro IPR012337 Domain
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![[124-335]T 5.0000090991531485E-40 PF01609 Transposase_11](http://www.ebi.ac.uk/InterProScan/images/H.gif){kind=small}
![[185-350]T 2.3000000000000003E-22 SSF53098 RNaseH_fold](http://www.ebi.ac.uk/InterProScan/images/B.gif){kind=small}
COGS Summary: COGS Search
No hit to the COGs database.
Blocks Summary: Blocks Search
***** PF01609 (Transposase (IS4 family)) with a combined E-value of 1.4e-08.
PF01609B 288-309
ProDom Summary: Protein Domain Search
Residues 271-336 are 45% similar to a (TRANSPOSASE PUTATIVE PLASMID ELEMENT) protein domain (PD003301 which is seen in Y4ZB_RHISN.
Paralogs: Local Blast Search
PG1447 is identical to PG0868, PG0445, PG0205, and PG0162, and is essentially identical to PG1461, PG1114, PG1920, PG0043, and PG0016, all predicted ISPg4-related transposases.
Pfam Summary: Pfam Search
Residues 124 to 335 (E-value = 6.9e-43) place PG1447 in the Transposase_11 family which is described as Transposase DDE domain (PF01609)
Structural Feature(s):
| Feature Type | Start | Stop |
| uncleavable signal | ||
| transmembrane |
Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).
Gene Protein Sequence:
MSTNISLFAQVIRLLPRPLIKKLSTEFQVDKHSKHFTSWQHLVSMIFSQF
SSCISLREISNGLRSATGNLNHLGICTAPSKSNLSYQNEHRTSDFFRACY
YALLDYFGQQGMGQGRKFRFKQPVKLLDSTTITLCLALYDWAKYTHTKGA
VKLHTLLDFKTLLPEYVHISDGKGHDGKMANSIPIPAGSIVVADRGYADT
ALLNSWDSTQVSFVVRHPRSLKYEVIQELELPEHGHQQILVDQRVRLTGV
QTQGKYTKPLRHIALYNEQHGDVVELLTNIDTLAASSIALLYRSRWLIEI
FFRNLKQRLSMKAFLGTTRNAVEVQIWTALITMLLMVYLKSIAKYRWCLS
NLVSSLRINTFTKMDLMQWLNEPFTPPPEPENQLF
Gene Nucleotide Sequence: Sequence Viewer
ATGAGTACAAATATAAGCCTATTCGCCCAAGTTATCCGCCTGTTGCCTCG
TCCATTGATTAAGAAGCTCTCTACAGAGTTTCAGGTAGACAAGCACAGCA
AGCATTTCACCTCATGGCAGCACTTAGTGAGCATGATTTTCAGCCAATTC
TCAAGCTGTATCTCCTTACGCGAGATTAGTAATGGCTTACGCTCAGCCAC
AGGTAACCTCAACCACTTAGGCATATGCACAGCACCCTCTAAGTCCAATT
TATCTTATCAGAACGAGCACCGTACCTCAGACTTCTTTCGAGCGTGCTAT
TATGCCTTGCTAGATTATTTCGGACAGCAGGGTATGGGGCAAGGGCGTAA
GTTTCGTTTTAAGCAACCCGTCAAGCTTCTAGACTCTACGACCATTACCC
TTTGCCTTGCCCTATACGATTGGGCTAAATACACACATACGAAGGGAGCT
GTTAAGCTTCATACACTCTTAGACTTCAAGACTCTGTTGCCTGAATATGT
GCATATTAGTGATGGTAAAGGACATGATGGCAAGATGGCCAATTCTATTC
CTATCCCAGCAGGAAGTATCGTCGTGGCTGACCGTGGCTATGCTGATACC
GCCTTGCTCAATAGTTGGGACAGCACCCAAGTGAGTTTTGTAGTACGCCA
CCCAAGGTCCTTAAAATATGAGGTTATTCAAGAGTTGGAACTCCCAGAAC
ATGGGCATCAGCAAATACTCGTAGATCAGAGAGTACGGCTCACAGGCGTT
CAGACGCAAGGCAAGTACACTAAGCCATTGAGGCATATTGCCCTCTACAA
CGAGCAACATGGCGATGTCGTTGAGTTATTGACCAACATAGACACCCTTG
CAGCAAGTAGTATAGCCTTGCTGTATCGTTCAAGATGGCTTATCGAGATT
TTCTTTCGGAATTTGAAGCAACGCCTCAGCATGAAGGCCTTTCTTGGAAC
AACGCGCAATGCCGTAGAGGTGCAAATATGGACGGCTCTTATTACAATGC
TTTTGATGGTCTATCTCAAGAGTATCGCCAAATATCGGTGGTGCTTGTCT
AATCTTGTCTCTTCGCTAAGAATCAACACATTTACCAAGATGGATCTGAT
GCAATGGCTTAATGAGCCGTTTACGCCTCCGCCAGAGCCCGAAAATCAGC
TCTTT