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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1269 IGR1270 IGR1268 IGR1272 IGR1274 IGR1273 IGR1271 PG1487 PG1489 PG1486 dsbD, - PG1485 PG1492 PG1493 ISPg5, - PG1490 ISPg5, - PG1491 PG1487 PG1489 PG1486 dsbD, - PG1485 PG1492 PG1493 ISPg5, - PG1490 ISPg5, - PG1491 Type: IS element, Name: ISPg5 - 8 PG1489 PG1486 dsbD, - PG1485 PG1492 PG1493 PG1487 ISPg5, - PG1490 ISPg5, - PG1491


LANL Gene ID: PG1491

GenBank Locus Tag: PG1710

DNA Molecule Name:
1  

Genomic Island ID:
15

GenBank ID:
34541341

 Gene Name:
ISPg5  

 Definition:
ISPg5 transposase

 Cellular Location:
Cytoplasm [Evidence]

 Gene Start:
1795500

Gene Stop:
1796435

Gene Length:
936

Molecular Weight*:
36302

pI*:
9.90

Net Charge*:
13.52

EC:
 

 Functional Class:
Transposon functions  

 Gene Ontology:
Biological process
  GO:0015074    DNA integration

Molecular function
  GO:0003677    DNA binding


 Pathway: pathway table

 Primary Evidence:
Califano,J.V., Kitten,T., Lewis,J.P., Macrina,F.L., Fleischmann,R.D., Fraser,C.M.,Duncan,M.J. and Dewhirst,F.E. Characterization of Porphyromonas gingivalis insertion
sequence-like element ISPg5.
Infect. Immun. 68 (9), 5247-5253 (2000)

 Comment:
One of eleven ISPg5 elements in the genome (see Paralogs). Califano et al. report considerable strain diversity of these (Infect. Immun. 68 (9), 5247-5253 (2000)).

Member of the IS3 family of insertion elements. See PG1490 for associated ORF.

TIGR ID: PG1710

 Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG1491 is equivalent to the previously reported gb|AAF69128.1| in GenBANK.

Numerous significant hits in gapped BLAST to transposase proteins; e.g. residues 91-213 are 56% similar to transposase homolog (U67062) of Bacteriodes thetaiotaomicrom, residues 64-268 are (33%) similar to InsB (AF153317) of Shigella dysenteriae, residues 64-268 are 33% similar to Hp1 (AF081284) of E.coli.

 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34541341|ref|NP_905820.1|  ISPg5, transposase Orf2 [Porph...   634   e-180
 gi|34540248|ref|NP_904727.1|  ISPg5, transposase Orf2 [Porph...   630   e-179
 gi|34539915|ref|NP_904394.1|  ISPg5 transposase Orf2 [Porphy...   622   e-177
 gi|34539888|ref|NP_904367.1|  ISPg5 transposase Orf2 [Porphy...   621   e-176
 gi|34540399|ref|NP_904878.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34540277|ref|NP_904756.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34541287|ref|NP_905766.1|  ISPg5, transposase Orf2 [Porph...   615   e-175
 gi|118200270|dbj|BAF37077.1|  transposase Orf2 [Porphyromona...   612   e-174
 gi|34540696|ref|NP_905175.1|  ISPg5, transposase Orf2 [Porph...   610   e-173
 gi|34541641|ref|NP_906120.1|  ISPg5, transposase Orf2 [Porph...   610   e-173


InterPro Summary:  InterProScan

InterPro
IPR001584
Domain
Integrase, catalytic core
PF00665 [110-273]T 8.1e-31 PF00665 rve rve
PS50994 [110-276]T 18.357 PS50994 INTEGRASE INTEGRASE
noIPR
unintegrated
unintegrated
G3DSA:3.30.420.10 [113-281]T 2.9e-06 G3DSA:3.30.420.10 no description no description


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01836 (Transposase) with a combined E-value of 4.3e-23.
    PF01836B    103-154
    PF01836C    181-190
    PF01836D    215-222
    PF01836E    253-272


 ProDom Summary:  Protein Domain Search
No significant hit to the ProDom database.

Paralogs:  Local Blast Search
PG1491 is identical to PG0391, essentially identical to PG0034, and is paralogously related to PG1861, PG0007, PG0418, PG1436, PG1796, PG0533, PG1247 and PG0841, all ISPg5 transposases.

Pfam Summary:  Pfam Search
Residues 110 to 273 (E-value = 8.3e-31) place PG1491 in the rve family which is described as Integrase core domain (PF00665)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
LLGFSRQAFYKRHLNDLAQHEEDVLCSSIIQYCWHLRQAEHLPQAGFREL
MVLCQQYFGPKFTLGRDRFCALLRRHGMMLRKRSVRPRTTNSRHRLYKYE
DLLNTEPKFVPQRPGELLVADITYVAYQDGFAYLSLLTDAYSRCIVGYCL
HPTLEVEGCLNALHQAFAFYDQHQIDTSNMIHHSDRGIQYAGKSYTDLLH
GRGWRISMTQTGDPLHNALAERMNNTLKNSWHISSSKQSFDQALLSVEQA
VRMYNEARPHQALGAKTPMQVIAPESKNPLLARIEHWPEIAPELYRRMNV
RQRANFARVNRN

Gene Nucleotide Sequence:  Sequence Viewer
CTGCTTGGCTTTAGCCGTCAGGCCTTTTATAAACGTCATCTCAACGATTT
AGCACAACATGAGGAGGATGTCCTATGCAGTAGTATCATCCAATATTGTT
GGCACTTAAGACAAGCAGAGCACCTACCTCAAGCCGGCTTCCGAGAGCTC
ATGGTGCTTTGTCAGCAATACTTCGGCCCCAAGTTCACGCTTGGGCGCGA
TCGCTTTTGCGCCCTACTCAGACGCCATGGTATGATGCTGCGCAAACGGT
CTGTGCGCCCTCGGACAACCAACTCTCGGCATAGGCTCTACAAGTATGAA
GACCTGCTCAATACAGAGCCTAAGTTTGTGCCTCAAAGACCTGGGGAACT
CCTTGTAGCAGACATCACCTACGTGGCTTACCAAGATGGGTTTGCCTATC
TCTCCCTACTGACCGATGCCTATAGTCGCTGTATCGTAGGGTATTGCTTA
CATCCGACCCTGGAAGTTGAAGGCTGTTTGAATGCTCTACATCAAGCCTT
TGCTTTCTACGATCAGCATCAAATTGATACGAGCAATATGATTCATCATA
GCGACCGAGGCATTCAGTATGCCGGTAAGAGCTACACCGATCTGTTGCAT
GGGCGAGGCTGGCGCATCAGCATGACTCAGACGGGAGATCCTCTGCATAA
TGCATTGGCTGAGCGGATGAACAATACGCTCAAGAACTCGTGGCACATCT
CCTCTTCGAAGCAGTCTTTTGATCAAGCACTCCTTTCTGTAGAACAAGCC
GTGCGCATGTACAACGAGGCGCGTCCACATCAAGCTCTAGGGGCGAAAAC
GCCTATGCAAGTCATTGCGCCAGAGTCTAAAAATCCGTTACTGGCAAGGA
TCGAACATTGGCCGGAGATTGCCCCCGAGCTATACCGAAGGATGAATGTC
AGACAAAGAGCTAACTTTGCTCGTGTCAACCGAAAT

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