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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1366 IGR1370 IGR1368 IGR1369 IGR1367 IGR1365 PG1609 spo, - PG1610 ISPg1, - PG1607 PG1603 PG1604 hagD,prtK,prtP,kgp, - PG1605 PG1609 spo, - PG1610 ISPg1, - PG1607 PG1603 PG1604 hagD,prtK,prtP,kgp, - PG1605 Type: IS element, Name: ISPg1 fragment - 3 Type: IS element, Name: ISPg1 fragment - 3 PG1609 spo, - PG1610 PG1603 PG1604 hagD,prtK,prtP,kgp, - PG1605 ISPg1, - PG1608 ISPg1, - PG1607 ISPg1, - PG1608


LANL Gene ID: PG1605

GenBank Locus Tag: PG1844

DNA Molecule Name:
1  

GenBank ID:
0

Gene Name:
hagD  prtK  prtP  kgp  

Definition:
porphypain polyprotein; lys-X proteinase/hemagglutinin

Cellular Location:
Extracellular [Evidence]

Gene Start:
1939824

Gene Stop:
1945016

Gene Length:
5193

Molecular Weight*:
187827

pI*:
5.10

Net Charge*:
-33.45

EC:
3.4.22.37  

Functional Class:
Cellular processes; Pathogenesis  

Gene Ontology:
Biological process
  GO:0006260    DNA replication
  GO:0006281    DNA repair
  GO:0006310    DNA recombination
  GO:0006508    proteolysis
  GO:0009405    pathogenesis

Molecular function
  GO:0003910    DNA ligase (ATP) activity
  GO:0005524    ATP binding
  GO:0008233    peptidase activity
  GO:0008234    cysteine-type peptidase activity


Pathway: pathway table

Primary Evidence:
See:

Proteases/Hemagglutinin Analysis.




Sato K, Sakai E, Veith PD, Shoji M, Kikuchi Y, Yukitake H, Ohara N, Naito M, Okamoto K, Reynolds EC, Nakayama K.
Identification of a New Membrane-associated Protein That Influences Transport/Maturation of Gingipains and Adhesins of Porphyromonas gingivalis.
J Biol Chem. 2005 Mar;280(10):8668-77.
PMID: 15634642

Veith PD, Chen YY, Reynolds EC.
Porphyromonas gingivalis RgpA and Kgp proteinases and adhesins are C terminally processed by the carboxypeptidase CPG70.
Infect Immun. 2004 Jun;72(6):3655-7.
PMID: 15155679

O'Brien-Simpson NM, Veith PD, Dashper SG, Reynolds EC.     
Antigens of bacteria associated with periodontitis.
Periodontol 2000. 2004;35:101-34.
PMID: 15107060

O-Brien-Simpson NM, Veith PD, Dashper SG, Reynolds EC.
Porphyromonas gingivalis gingipains: the molecular teeth of a microbial vampire.
Curr Protein Pept Sci. 2003 Dec;4(6):409-26.
PMID: 14683427

Veith PD, Talbo GH, Slakeski N, Dashper SG, Moore C, Paolini RA, Reynolds EC.
Major outer membrane proteins and proteolytic processing of RgpA and Kgp of Porphyromonas gingivalis W50.
Biochem J. 2002 Apr;363(Pt 1):105-15.
PMID: 11903053

Slakeski N, Cleal SM, Bhogal PS, Reynolds EC.
Characterization of a Porphyromonas gingivalis gene prtK that encodes a lysine-specific cysteine proteinase and three sequence-related adhesins.
Oral Microbiol Immunol. 1999 Apr;14(2):92-7.
PMID: 10219167

Comment:
Porphypain is thought to possess both Arg-X and Lys-X proteinase activity in strain W12.(Barkocy-Gallagher et al.). Slakeski et al. report that this protein is a polyprotein consisting (after postranslational modification) of prtK48, the Lys-specific cysteine proteinase, and three sequence related adhesins, PrtK39, PrtK15 and PrtK44 in strain W50. Both sequences are 1732 amino acids. The sequence herein, 1542 amino acids, is from strain W83.

Han et al. (U68468) characterize the first 363 amino acids to be protease whereas the next 1090 to be hemagglutinin (hagD).

Gene nomenclature and strains (from Lamont and Jenkinson): kgp (HG66), prtK(W50), prtP(W12).

Veith et al. 2002 identified this protein by two-dimensional gel electrophoresis and peptide mass fingerprinting of outer membranes prepared from strain W50. They present the following results: Based on signal peptide sequence comparisons to PG0626, PG0627, PG1592, PG1840, PG0836, PG0023, PG1893, the mature N-terminus of this protein is predicted to be pyroglutamate. RgpA, Kgp and HagA were identified as fully processed domains. The N- and C-termini of many of these domains were identified by mass spectrometry and or N-terminal sequence analysis. It was found that gaps existed between many of the domains. The proposed domain structure of Kgp is as follows: Kgp signal peptide (1-19), Kgp pro-domain (20-228), Kgp48 (229-710), Kgp39 (738-1099), Kgp15 (1157-1275), Kgp14 (1292-1424), Kgp13 (1427-1546), Kgp20 (1548-1732).

The T1a protein, PG0581, PG0582, is virtually identical to the C-terminal half of PrtK.

For the Arg-X proteinases, see PG1768 and PG0461.

TIGR ID: PG1844

Proteomic Data: Proteomic Data Search
TIGR Annotationlysine-specific cysteine proteinase, authentic frameshift, GroupD (cellular processes) ,GroupM (protein fate)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG1844-1.060-0.570.0044-1.510-0.570.0044
LANLPG16050.480.670.350.82
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
This sequence corresponds to 3869077 in Genbank.



 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|2182812|gb|AAB60809.1|  lysine-specific cysteine proteina...  3316   0.0  
 gi|1314326|gb|AAB06565.1|  porphypain                            3313   0.0  
 gi|2738803|gb|AAC26523.1|  lysine specific cysteine protease...  3304   0.0  
 gi|1536824|dbj|BAA11870.1|  Lys-gingipain [Porphyromonas gin...  2905   0.0  
 gi|1314751|gb|AAA99810.1|  Lys-gingipain                         2790   0.0  
 gi|45593253|gb|AAS68176.1|  Lys-gingipain [Porphyromonas gin...  2458   0.0  
 gi|3869077|dbj|BAA34341.1|  130k-HMGD [Porphyromonas gingiva...  2323   0.0  
 gi|1890077|gb|AAB49691.1|  hemagglutinin [Porphyromonas ging...  2211   0.0  
 gi|1514621|emb|CAA68897.1|  TonB-linked adhesin [Porphyromon...  1533   0.0  
 gi|34541613|ref|NP_906092.1|  hemagglutinin protein HagE [Po...  1489   0.0


InterPro Summary:  InterProScan

InterPro
IPR000977
Family
ATP-dependent DNA ligase
PS00697 [1149-1157]? NA PS00697 DNA_LIGASE_A1 DNA_LIGASE_A1
InterPro
IPR001769
Family
Peptidase C25, gingipain
PF01364 [231-598]T 1.3e-299 PF01364 Peptidase_C25 Peptidase_C25
InterPro
IPR003961
Domain
Fibronectin, type III
SM00060 [945-1073]T 23 SM00060 FN3[1398-1649]T 1.7e+02 SM00060 FN3 FN3
InterPro
IPR005536
Domain
Peptidase C25, C-terminal ig-like region
PF03785 [600-680]T 1.5e-54 PF03785 Peptidase_C25_C Peptidase_C25_C
InterPro
IPR011628
Domain
Cleaved adhesin
PF07675 [982-1150]T 2.3e-117 PF07675 Cleaved_Adhesin[1159-1330]T 1e-114 PF07675 Cleaved_Adhesin[1427-1598]T 8.5e-113 PF07675 Cleaved_Adhesin Cleaved_Adhesin
noIPR
unintegrated
unintegrated
G3DSA:3.40.50.1460 [354-597]T 1.5e-58 G3DSA:3.40.50.1460 no description no description
signalp [1-21]? NA signalp signal-peptide signal-peptide


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01364 (Peptidase family C25) with a combined E-value of 9.5e-125.
    PF01364B    709-716
    PF01364C    717-770
    PF01364D    793-830
    PF01364E    846-896
    PF01364F    916-969


 ProDom Summary:  Protein Domain Search
Residues 1100-1132 are 84% similar to a (PROTEASE PRTH EC 3.4.22.-) protein domain (PD125301 which is seen in PRTH_PORGI.

Residues 1338-1393 are identical to a (PROTEASE PRECURSOR SIGNAL THIOL HEMAGGLUTININ REPEAT) protein domain (PD002028 which is seen in P72196_PORGI.

Residues 514-673 are identical to a (CYSTEINE PROTEASE LYS-GINGIPAIN) protein domain (PD011535 which is seen in O07442_PORGI.

Residues 18-224 are identical to a (PROTEASE CYSTEINE PRECURSOR THIOL) protein domain (PD005390 which is seen in Q51817_PORGI.

Residues 1394-1476 are identical to a (PROTEASE PRECURSOR SIGNAL HEMAGGLUTININ THIOL REPEAT) protein domain (PD001858 which is seen in P72196_PORGI.

Residues 444-513 are identical to a (PROTEASE PROTEINASE CYSTEINE PRECURSOR) protein domain (PD007636 which is seen in O07442_PORGI.

Residues 1327-1386 are 90% similar to a (PROTEASE THIOL REPEAT PRECURSOR) protein domain (PD013956 which is seen in Q51839_PORGI.

Residues 243-443 are identical to a (PROTEASE THIOL CYSTEINE PRECURSOR) protein domain (PD005006 which is seen in O52050_PORGI.


Paralogs:  Local Blast Search
PG1605 is similar to PG1768, PG1602, PG1164, PG0461, PG1606, PG0376, PG1922, and PG0375, in order of decreasing similarity. Residues 6-142 are 54% similar to residues 10-1408 of PG1768, a predicted gingipain R1 sequence (an arginine-specific cysteine proteinase); near-identity is observed for the C-terminal halves of these proteins. Residues 883-1476 are 87% similar to residues 1317-1908 of PG1602, a predicted HAGA protein. Thus the C-terminal portions of PG1605 and PG1768 are similar to this region of HAGA. The C-terminal portion of PG1605 is virtually identical to the T1a protein, PG0581 and PG0582.

Pfam Summary:  Pfam Search
Residues 231 to 598 (E-value = 1.2e-299) place PG1605 in the Peptidase_C25 family which is described as Peptidase family C25 (PF01364)
Residues 600 to 680 (E-value = 2e-54) place PG1605 in the Peptidase_C25_C family which is described as Peptidase family C25, C terminal ig-like domain (PF03785)

 Structural Feature(s):
Feature Type  Start  Stop
cleavable signal  
1  
21
non-globular  
43  
107
transmembrane  
1474  
1491

Top PDB Hits:
-42% similar to PDB:1CVR CRYSTAL STRUCTURE OF THE ARG SPECIFIC CYSTEINE PROTEINASE GINGIPAIN R (RGPB) (E_value = 9.9E_32);
-41% similar to PDB:1GW0 CRYSTAL STRUCTURE OF LACCASE FROM MELANOCARPUS ALBOMYCES IN FOUR COPPER FORM (E_value = 9.9E_32);
-41% similar to PDB:2IH8 A low-dose crystal structure of a recombinant Melanocarpus albomyces laccase (E_value = 9.9E_32);
-41% similar to PDB:2IH9 A high-dose crystal structure of a recombinant Melanocarbus albomyces laccase (E_value = 9.9E_32);
-67% similar to PDB:1KFI Crystal Structure of the Exocytosis-Sensitive Phosphoprotein, pp63/Parafusin (phosphoglucomutase) from Paramecium (E_value = 9.9E_32);

Gene Protein Sequence:
MRKLLLLIAASLLGVGLYAQSAKIKLDAPTTRTTCTNNSFKQFDASFSFN
EVELTKVETKGGTFASVSIPGAFPTGEVGSPEVPAVRKLIAVPVGATPVV
RVKSFTEQVYSLNQYGSEKLMPHQPSMSKSDDPEKVPFVYNAAAYARKGF
VGQELTQVEMLGTMRGVRIAALTINPVQYDVVANQLKVRNNIEIEVSFQG
ADEVATQRLYDASFSPYFETAYKQLFNRDVYTDHGDLYNTPVRMLVVAGA
KFKEALKPWLTWKAQKGFYLDVHYTDEAEVGTTNASIKAFIHKKYNDGLA
ASAAPVFLALVGDTDVISGEKGKKTKKVTDLYYSAVDGDYFPEMYTFRMS
ASSPEELTNIIDKVLMYEKATMPDKSYLEKVLLIAGADYSWNSQVGQPTI
KYGMQYYYNQEHGYTDVYNYLKAPYTGCYSHLNTGVSFANYTAHGSETAW
ADPLLTTSQLKALTNKDKYFLAIGNCCITAQFDYVQPCFGEVITRVKEKG
AYAYIGSSPNSYWGEDYYWSVGANAVFGVQPTFEGTSMGSYDATFLEDSY
NTVNSIMWAGNLAATHAGNIGNITHIGAHYYWEAYHVLGDGSVMPYRAMP
KTNTYTLPASLPQNQASYSIQASAGSYVAISKDGVLYGTGVANASGVATV
SMTKQITENGNYDVVITRSNYLPVIKQIQVGEPSPYQPVSNLTATTQGQK
VTLKWEAPSAKKAEGSREVKRIGDGLFVTIEPANDVRANEAKVVLAADNV
WGDNTGYQFLLDADHNTFGSVIPATGPLFTGTASSNLYSANFEYLIPANA
DPVVTTQNIIVTGQGEVVIPGGVYDYCITNPEPASGKMWIAGDGGNQPAR
YDDFTFEAGKKYTFTMRRAGMGDGTDMEVEDDSPASYTYTVYRDGTKIKE
GLTATTFEEDGVAAGNHEYCVEVKYTAGVSPKVCKDVTVEGSNEFAPVQN
LTGSSVGQKVTLKWDAPNGTPNPNPNPNPNPGTTLSESFENGIPASWKTI
DADGDGHGWKPGNAPGIAGYNSNGCVYSESFGLGGIGVLTPDNYLITPAL
DLPNGGKLTFWVCAQDANYASEHYAVYASSTGNDASNFTNALLEETITAK
GVRSPKAIRGRIQGTWRQKTVDLPAGTKYVAFRHFQSTDMFYIDLDEVEI
KANGKRADFTETFESSTHGEAPAEWTTIDADGDGQGWLCLSSGQLDWLTA
HGGSNVVSSFSWNGMALNPDNYLISKDVTGATKVKYYYAVNDGFPGDHYA
VMISKTGTNAGDFTVVFEETPNGINKGGARFGLSTEANGAKPQSVWIERT
VDLPAGTKYVAFRHYNCSDLNYILLDDIQFTMGGSPTPTDYTYTVYRDGT
KIKEGLTETTFEEDGVATGNHEYCVEVKYTAGVSPKKCVNVTVNSTQFNP
VQNLTAEQAPNSMDAILKWNAPASKRAEVLNEDFENGIPASWKTIDADGD
GNNWTTTPPPGGSSFAGHNSAICVSSASYINFEGPQNDNYLVTPELSLPG
GGTLTFWVCAQDANYASEHYAVYASSTGNDASNFANALLEEVLTAKTVVT
APEAIRGTRAQGTWYQKTVQLPAGTKYVAFRHFGCTDFFWINLDDVVITS
GNAPSYTYTIYRNNTQIASGVTETTYRDPDLATGFYTYGVKVVYPNGESA
IETATLNITSLADVTAQKPYTLTVVGKTITVTCQGEAMIYDMNGRRLAAG
RNTVVYTAQGGHYAVMVVVDGKSYVEKLAVK

Gene Nucleotide Sequence:  Sequence Viewer
ATGAGGAAATTATTATTGCTGATCGCGGCGTCCCTTTTGGGAGTTGGTCT
TTACGCCCAAAGCGCCAAGATTAAGCTTGATGCTCCGACTACTCGAACGA
CATGTACGAACAATAGCTTCAAGCAGTTCGATGCAAGCTTTTCGTTCAAT
GAAGTCGAGCTGACAAAGGTGGAGACCAAAGGTGGTACTTTCGCCTCAGT
GTCAATTCCGGGTGCATTCCCGACCGGTGAGGTTGGTTCTCCCGAAGTGC
CAGCAGTTAGGAAGTTGATTGCTGTGCCTGTCGGAGCCACACCTGTTGTT
CGCGTGAAAAGTTTTACCGAGCAAGTTTACTCTCTGAACCAATACGGTTC
CGAAAAACTCATGCCACATCAACCCTCTATGAGCAAGAGTGATGATCCCG
AAAAGGTTCCCTTCGTTTACAATGCTGCTGCTTATGCACGCAAAGGTTTT
GTCGGACAAGAACTGACCCAAGTAGAAATGTTGGGGACAATGCGTGGTGT
TCGCATTGCAGCTCTTACCATTAATCCTGTTCAGTATGATGTGGTTGCAA
ACCAATTGAAGGTTAGAAACAACATCGAAATTGAAGTAAGCTTTCAAGGA
GCTGATGAAGTAGCTACACAACGTTTGTATGATGCTTCTTTTAGCCCTTA
TTTCGAAACAGCTTATAAACAGCTCTTCAATAGAGATGTTTATACAGATC
ATGGCGACTTGTATAATACGCCGGTTCGTATGCTTGTTGTTGCAGGTGCA
AAATTCAAAGAAGCTCTCAAGCCTTGGCTCACTTGGAAGGCTCAAAAGGG
CTTCTATCTGGATGTGCATTACACAGACGAAGCTGAAGTAGGAACGACAA
ACGCCTCTATCAAGGCATTTATTCACAAGAAATACAATGATGGATTGGCA
GCTAGTGCTGCTCCGGTCTTCTTGGCTTTGGTTGGTGACACTGACGTTAT
TAGCGGAGAAAAAGGAAAGAAAACAAAAAAAGTTACCGACTTGTATTACA
GTGCAGTCGATGGCGACTATTTCCCTGAAATGTATACTTTCCGTATGTCT
GCTTCTTCCCCAGAAGAACTGACGAACATCATTGATAAGGTATTGATGTA
TGAAAAGGCTACTATGCCAGATAAGAGTTATTTGGAGAAAGTTCTCTTGA
TTGCAGGTGCAGATTATAGCTGGAATTCCCAGGTAGGTCAGCCAACCATT
AAATACGGTATGCAGTACTACTACAACCAAGAGCATGGTTATACCGACGT
GTACAACTATCTCAAAGCCCCTTATACAGGTTGCTACAGTCATTTGAATA
CCGGAGTCAGCTTTGCAAACTATACAGCGCATGGATCTGAGACCGCATGG
GCTGATCCACTTCTGACTACTTCTCAACTGAAAGCACTCACTAATAAGGA
CAAATACTTCTTAGCTATTGGCAACTGCTGTATTACAGCTCAATTCGATT
ATGTACAGCCTTGCTTCGGAGAGGTAATAACTCGCGTTAAGGAGAAAGGG
GCTTATGCCTATATCGGTTCATCTCCAAATTCTTATTGGGGCGAGGACTA
CTATTGGAGTGTGGGTGCTAATGCCGTATTTGGTGTTCAGCCTACTTTTG
AAGGTACGTCTATGGGTTCTTATGATGCTACATTCTTGGAGGATTCGTAC
AACACAGTGAATTCTATTATGTGGGCAGGTAATCTTGCCGCTACTCATGC
TGGAAATATCGGCAATATTACCCATATTGGTGCTCATTACTATTGGGAAG
CTTATCATGTCCTTGGCGATGGTTCGGTTATGCCTTATCGTGCAATGCCT
AAGACCAATACTTATACGCTTCCTGCCTCTTTGCCTCAGAATCAGGCTTC
TTATAGCATTCAGGCTTCTGCCGGTTCTTACGTAGCTATTTCTAAAGATG
GAGTTTTGTATGGAACAGGTGTTGCTAATGCCAGCGGTGTTGCGACTGTG
AGTATGACTAAGCAGATTACGGAAAATGGTAATTATGATGTAGTTATCAC
TCGCTCTAATTATCTTCCTGTGATCAAGCAAATTCAGGTAGGTGAGCCTA
GCCCCTACCAGCCCGTTTCCAACTTGACAGCTACAACGCAGGGTCAGAAA
GTAACGCTCAAGTGGGAAGCACCGAGCGCAAAGAAGGCAGAAGGTTCCCG
TGAAGTAAAACGGATCGGAGACGGTCTTTTCGTTACGATCGAACCTGCAA
ACGATGTACGTGCCAACGAAGCCAAGGTTGTGCTTGCGGCAGACAACGTA
TGGGGAGACAATACGGGTTACCAGTTCTTGTTGGATGCCGATCACAATAC
ATTCGGAAGTGTCATTCCGGCAACCGGTCCTCTCTTTACCGGAACAGCTT
CTTCCAATCTTTACAGTGCGAACTTCGAGTATTTGATCCCGGCCAATGCC
GATCCTGTTGTTACTACACAGAATATTATCGTTACAGGACAGGGTGAAGT
TGTAATCCCCGGTGGTGTTTACGACTATTGCATTACGAACCCGGAACCTG
CATCCGGAAAGATGTGGATCGCAGGAGATGGAGGCAACCAGCCTGCACGT
TATGACGATTTCACATTCGAAGCAGGCAAGAAGTACACCTTCACGATGCG
TCGCGCCGGAATGGGAGATGGAACTGATATGGAAGTCGAAGACGATTCAC
CTGCAAGCTATACCTACACGGTGTATCGTGACGGCACGAAGATCAAGGAA
GGTCTGACAGCTACGACATTCGAAGAAGACGGTGTAGCTGCAGGCAATCA
TGAGTATTGCGTGGAAGTTAAGTACACAGCCGGCGTATCTCCGAAGGTAT
GTAAAGACGTTACGGTAGAAGGATCCAATGAATTTGCTCCTGTACAGAAC
CTGACCGGTAGTTCAGTAGGTCAGAAAGTAACGCTTAAGTGGGATGCACC
TAATGGTACCCCGAATCCGAATCCAAATCCGAATCCGAATCCGGGAACAA
CACTTTCCGAATCATTCGAAAATGGTATTCCGGCATCTTGGAAGACGATC
GATGCAGACGGTGACGGGCATGGCTGGAAACCTGGAAATGCTCCCGGAAT
CGCTGGCTACAATAGCAATGGTTGTGTATATTCAGAGTCATTCGGTCTTG
GTGGTATAGGAGTTCTTACCCCTGACAACTATCTGATAACACCGGCATTG
GATTTGCCTAACGGAGGTAAGTTGACTTTCTGGGTATGCGCACAGGATGC
TAATTATGCATCCGAGCACTATGCGGTGTATGCATCTTCGACCGGTAACG
ATGCATCCAACTTCACGAATGCTTTGTTGGAAGAGACGATTACGGCAAAA
GGTGTTCGCTCGCCGAAAGCTATTCGTGGTCGTATACAGGGTACTTGGCG
CCAGAAGACGGTAGACCTTCCCGCAGGTACGAAATATGTTGCTTTCCGTC
ACTTCCAAAGCACGGATATGTTCTACATCGACCTTGATGAGGTTGAGATC
AAGGCCAATGGCAAGCGCGCAGACTTCACGGAAACGTTCGAGTCTTCTAC
TCATGGAGAGGCACCAGCGGAATGGACTACTATCGATGCCGATGGCGATG
GTCAGGGTTGGCTCTGTCTGTCTTCCGGACAATTGGACTGGCTGACAGCT
CATGGCGGCAGCAACGTAGTAAGCTCTTTCTCATGGAATGGAATGGCTTT
GAATCCTGATAACTATCTCATCTCAAAGGATGTTACAGGCGCAACGAAGG
TAAAGTACTACTATGCAGTCAACGACGGTTTTCCCGGGGATCACTATGCG
GTGATGATCTCCAAGACGGGCACGAACGCCGGAGACTTCACGGTTGTTTT
CGAAGAAACGCCTAACGGAATAAATAAGGGCGGAGCAAGATTCGGTCTTT
CCACGGAAGCCAATGGCGCCAAACCTCAAAGTGTATGGATCGAGCGTACG
GTAGATTTGCCTGCAGGCACGAAGTATGTTGCTTTCCGTCACTACAATTG
CTCGGATTTGAACTACATTCTTTTGGATGATATTCAGTTCACCATGGGTG
GCAGCCCCACCCCGACCGATTATACCTACACGGTGTATCGTGATGGTACG
AAGATCAAGGAAGGTTTGACCGAAACGACCTTCGAAGAAGACGGCGTAGC
TACGGGCAATCATGAGTATTGCGTGGAAGTGAAGTACACAGCCGGCGTAT
CTCCGAAGAAATGTGTAAACGTAACTGTTAATTCGACACAGTTCAATCCT
GTACAGAACCTGACGGCAGAACAAGCTCCTAACAGCATGGATGCAATCCT
TAAATGGAATGCACCGGCATCTAAGCGTGCGGAAGTTCTGAACGAAGACT
TCGAAAATGGTATTCCTGCCTCATGGAAGACGATCGATGCAGACGGTGAC
GGCAACAATTGGACGACGACCCCTCCTCCCGGAGGCTCCTCTTTTGCAGG
TCACAACAGTGCGATCTGTGTCTCTTCAGCTTCTTATATCAACTTTGAAG
GTCCTCAGAATGATAACTATCTGGTTACACCGGAGCTTTCTCTTCCTGGC
GGAGGAACGCTTACTTTCTGGGTATGTGCACAAGATGCCAATTATGCATC
AGAGCACTATGCCGTGTACGCATCTTCTACGGGTAACGACGCTTCCAACT
TCGCCAACGCTTTGTTGGAAGAAGTGCTGACGGCCAAGACAGTTGTTACG
GCACCTGAAGCCATTCGTGGTACTCGTGCTCAGGGCACCTGGTATCAAAA
GACGGTACAGTTGCCTGCGGGTACTAAGTATGTTGCCTTCCGTCACTTCG
GCTGTACGGACTTCTTCTGGATCAACCTTGATGATGTTGTAATCACTTCA
GGGAACGCTCCGTCTTACACCTATACGATCTATCGTAATAATACACAGAT
AGCATCAGGCGTAACGGAGACTACTTACCGAGATCCGGACTTGGCTACCG
GTTTTTACACGTACGGTGTAAAGGTTGTTTACCCGAACGGAGAATCAGCT
ATCGAAACTGCTACGTTGAATATCACTTCGTTGGCAGACGTAACGGCTCA
GAAGCCTTACACGCTGACAGTTGTAGGAAAGACGATCACGGTAACTTGCC
AAGGCGAAGCTATGATCTACGACATGAACGGTCGTCGTCTGGCAGCGGGT
CGCAACACGGTTGTTTACACGGCTCAGGGCGGCCACTATGCAGTCATGGT
TGTCGTTGACGGCAAGTCTTACGTAGAGAAACTCGCTGTAAAG

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